JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE
·
2019
·Vol. 144
·期 6
·420-428
·
IF 0.829
Yu Wang,Haobo Yang,Shuai Zhong,Xin Liu,Tong Li,Chengwen Zong
Sugar and organic acid contents as well as sugar-to-acid ratio of fruit are important factors affecting fruit quality and processing. In this study, we compared sugar and organic acid contents of Vaccinium uliginosum (bog bilberry) fruit harvested from 100 plants across 10 wild populations growing in the Changbai Mountains of China. Correlation of sugar and acid contents with the spatial distribution of these populations was accessed. Germplasm with high sugar and low acid with potential for use in future breeding was also identified. Results showed that sugar and organic acid contents varied among different V. uliginosum populations in the Changbai Mountains. Fructose (18.60–38.54 mg·g−1) and glucose (15.77–35.08 mg·g−1) were the main sugars, whereas quinic (16.06–20.97 mg·g−1), citric (10.39–14.96 mg·g−1), and malic (4.28–7.47 mg·g−1) acids were the main organic acids in V. uliginosum. The first population collected in the Dongfanghong forest farm (lat. 42°00′N, long. 128°03′E) showed the highest average sugar content and sugar-to-acid ratio among the sampled populations. Moreover, total sugar content and sugar-to-acid ratio of Sample 7—Population 1, which is located at Manjiang forest farm (lat. 41°09′N, long. 127°05′E) was much higher than the other 99 samples, making this sample representative of important germplasm for breeding. Altitude and latitude were important environmental factors affecting sugar and organic acid contents. Fructose, glucose, and total sugar contents were positively correlated with altitude and negatively correlated with latitude. Oxalic acid content and sugar-to-acid ratio were positively correlated with altitude, and there was no significant correlation between organic acid contents and latitude.
研究领域:
植物学
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园艺学
✓ 已验证
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Peilin Chen,YuShan Huang,Hongliang Zeng,Mingjing Zheng,Juanjuan Guo
This study systematically investigated the modulatory effects of Porphyra haitanensis polysaccharides (PHP) and its two P. haitanensis polysaccharide components (PHP40 and PHP80) on gut microbiota in ovalbumin (OVA)-sensitized mice via in vitro fermentation. PHP40 and PHP80 exhibited sulfate contents of 5.94 ± 0.05 % and 11.72 ± 0.03 %, respectively, with galactose and glucose as dominant monosaccharides. The different polysaccharide components had different effects on the aforementioned changes in the species composition and structure of the gut microbiota in allergic mice, with distinct dominant microbial profiles across groups. PHP could promote Bacillus and Enterococcus proliferation while inhibiting Staphylococcus . PHP40 and PHP80 could promote Enterococcus and Enterobacter growth but suppressed Staphylococcus and Bacillus . Functional prediction indicated PHP significantly improved galactose metabolism and primary/secondary bile acid biosynthesis, potentially alleviating allergic responses.
研究领域:
微生物学
·
免疫学
·
营养学
✓ 已验证
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Na Yang,Yamei Jin,Yuyi Zhou,Xiaoqun Zhou
A novel electromagnetic heat extraction method was presented, whereby mandarin peels residue solution was located in a winding coil subjected to an oscillating magnetic field, and the pectin was extracted under appropriate conditions. Numerical relationships between applied magnetic field and induced electric field (IEF) in the extraction process were elaborated. The results showed that the induced current density, IEF and terminal temperature increased with increasing magnetic field. The maximum current density of 0.35 A/cm corresponds to the highest terminal temperature of 84.6 °C and IEF intensity of 26.6 V/cm. When magnetic field intensity was 1.39 T and the extraction time was 15 min, the maximum yield of pectin reached 9.16 %. In addition, all treatments impacted the ash content, protein content, water-holding capacity (WHC), and oil-holding capacity (OHC) of the obtained pectin. The pectin extracted by electromagnetic heat had the lowest DE value of 71.3 % with 126.55 kDa molecular weight, while the GalA content was at the highest level of 76.18 %. After different treatments, the composition of pectin monosaccharides changed, but there were slight differences in the composition of pectin polysaccharides. Moreover, the electromagnetic heat extracted pectin had light color and an obvious surface fragmentation of the peel residue.
研究领域:
食品科学
·
化学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CERAMICS INTERNATIONAL
·
2022
·
IF 5.532
Fei He,Wenjie Li,Tianliang Pang,Liang Zhou,Chen Wang,Hang Liu,Mingwei Li,Xiaodong He
Boehmite nanorods (BNRs) were synthesized via the sol-gel process and hydrothermal method from alumina sols. Four influence factors, i.e. hydrothermal temperature and time, acetic acid contents, alcohol contents and stirring patterns, were explored to understand the reasons of BNRs growth. Scanning electron microscopy (SEM), transmission electron microscope (TEM), Fourier transform infrared (FT-IR) spectra and X-ray diffraction (XRD) were used to investigate the micromorphology, functional groups and phase composites of BNRs. The results show that the hydrothermal temperature and the contents of acetic acid play important parts in the one-dimension (1D) growth of BNRs. However, the hydrothermal time and the contents of alcohol have little effects. The growth mechanism of BNRs is related to the formation, growth and directional assembling of boehmite crystallites . After the simulation of dissipative particle dynamics (DPD), the increase of shear rate is beneficial to the 1D growth of BNRs.
研究领域:
材料科学
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Dingding Liu,Kang Wei,Chenyu Zhang,Haoran Liu,Yang Gong,Yuanyuan Ye,Jiedan Chen,Mingzhe Yao,Liang Chen,Chunlei Ma
Albino tea has been receiving growing attention on the tea market due to its attractive appearance and fresh taste, mainly caused by high amino acid contents. Here, variations in the contents of five free amino acids in relation to pigment contents and tree age in two hybrid populations‘Longjin 43′(♀) × ‘Baijiguan’(♂) and ‘Longjin 43′(♀) בHuangjinya’(♂) with 334 first filial generation individuals including chlorophyll-deficient and normal tea plants were investigated. The data showed that the contents of main amino acids in all filial generation gradually decreased as plant age increased. Principal component analysis indicated that the amino acid content of individual plant tended to be stable with the growth of plants. Correlation analysis clarified that several main amino acids were significantly negatively correlated with chlorophyll a , chlorophyll b and carotenoid contents. Our results showed that the accumulation of amino acids in tea plant was closely related to leaf color variation and the tree age during growing period.
研究领域:
植物学
·
遗传学
·
园艺学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TOPICS IN CATALYSIS
·
2025
·
IF 3
Miao Wenlong,Wang Tian,Wang Dongbin,Le Thiquynhxuan,Zhang Libo
The development of high-efficiency and eco-friendly catalysts is critical for achieving sustainable and green transformation in the chemical industry. Carbon-based solid acids, prepared with activated carbon as a carrier and sulfuric acid as the active component, exhibit advantages such as good catalytic performance, low cost, easy separation, and environmental friendliness. However, existing methods for preparing carbon-based sulfonic acid solid acid catalysts often suffer from stringent synthesis conditions and complex procedures. In this work, a novel, highly efficient, and recyclable micron-sized carbon-based solid acid was synthesized by using ultrasonic pretreatment method. The results demonstrated that the carbon-based solid acid prepared by ultrasonic pretreatment had an acid content as high as 1.19 mmol/g, a specific surface area of 178.56 m 2 /g, and an average particle size of 32.548 μm. The introduction of ultrasound improved the surface structure of the activated carbon, enlarged the pore volume and increased the specific surface area. The carbon-based solid acid prepared under ultrasonic treatment showed a 57.43% increase in acid content and a 44.51% increase in specific surface area compared to samples prepared under conventional conditions. This work provides a new method for synthesizing high-performance, recyclable non-homogeneous catalysts with large particle sizes.
研究领域:
化学工程
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材料科学
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环境科学
✓ 已验证
产品关联
DOI
高影响因子
GLYCOCONJUGATE JOURNAL
·
2016
·Vol. 33
·期 2
·219-226
·
IF 1.828
Yao Hong L.,Conway Louis P.,Wang Mao M.,Huang Kun,Liu Li,Voglmeir Josef
Herein we describe a UPLC-FLD-based method for the quantification of the sialic acid content of red meat, using a synthetic neuraminic acid derivative as an internal standard. X-Gal-α-2,6- N -propionylneuraminic acid was synthesized via a chemoenzymatic pathway and its hydrolytic stability was characterized. Known quantities of this compound were incubated with samples of red meat under sialic acid-releasing conditions. The released sialic acids were derivatized, analyzed by UPLC-FLD, and the Neu5Ac/Neu5Gc content of the meat sample was determined by comparison with the internal standard. A number of red meats were analyzed by this method with the following results (Neu5Ac μg/g tissue, Neu5Gc μg/g tissue ± s.d.): pork (68 ± 3, 15.2 ± 0.7), beef (69 ± 8, 36 ± 5), lamb (46 ± 2, 33 ± 1), rabbit (59 ± 2, 0.4 ± 0.4), and hare (50 ± 4, 1 ± 1). We envisage that this methodology will find application in investigating the health effects of dietary Neu5Gc.
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研究领域:
生物化学
·
食品科学
·
医学营养学
✓ 已验证
DOI
PubMed
高影响因子
Journal of Energy Storage
·
2022
·
IF 8.907
Shiju Cai,Chenyi Wang,Jiafeng Qian,Zhengwang Tao,Jian Li
A series of aromatic proton exchange membranes (PAES-4S-xx) with tetrasulfonated side alkyl chains in repeated structural units are conveniently prepared by two-step reaction using 3,3′-(3″-aminophenyl)-4,4′-difluorodiphenylsulfone (2NH 2 -DFDPS), 4,4′-difluoro-diphenylsulfone, 4,4′-dihydroxybiphenyl and sodium 2-bromoethyl sulfonate as starting materials. Their chemical structure and physical properties are systematically studied. The incorporation of locally dense flexible side alkyl chains effectively endows the obtained ionic membranes based on PAES-4S-xx with high proton conductivity and moderate water uptake and swelling ratio. The corresponding values are between 38 and 98 mS/cm, 10 %–26 % and 6 %–18 % (30 °C), respectively, which increases with the increase of sulfonic acid content. In VRFB tests, the coulomb efficiency (CE) and energy efficiency (EE) of a single battery based on PAES-4S-24 membrane reach 95.8 % and 86.6 % at current density of 40 mA/cm 2 , respectively, higher than that of the single battery assembled by Nafion 115 (CE:90.7 %, EE:81.5 %). In addition, the single battery based on PAES-4S-24 also shows good stable performance during cyclic charging and discharging.
研究领域:
材料科学
·
电化学
✓ 已验证
产品关联
DOI
高影响因子
MATERIALS CHEMISTRY AND PHYSICS
·
2019
·
IF 2.781
Lei Wang,Chuyang Liu,Guoyue Xu,Shanshan Xiang,Mengyu Shi,Yujing Zhang
CeO 2 powders with flaky morphology and high floating rate are fabricated successfully by ball-milling and stearic acid modification. The influences of ball-milling time and surfactant stearic acid content on the morphology, floating rate, infrared emissivity of CeO 2 powders as well as the CeO 2 /epoxy-silicone coatings have been investigated systematically. The results show that the CeO 2 powders transform from irregular grains with larger size to flake grains with smaller size by ball-milling for 4 h. The floating rate of CeO 2 enhances from 0 cm 2 /g to 89.06 cm 2 /g by increasing stearic acid content from 0 wt% to 1 wt% while diminishes to 60.477 cm 2 /g with stearic acid content of 3 wt%. Ultimately, the coating with CeO 2 fillers ball-milled by 4 h and modified by 1 wt% of stearic acid exhibits the lowest emissivity value of 0.831 in the 8–14 μm waveband. The mechanisms for small and flaky fillers as well as high floating fillers to achieve low emissivity coatings are also fully revealed.
研究领域:
材料科学
·
表面工程
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2015
·
IF 4.169
Liu-Lian Huang,Hui-Chao Hu,Li-Hui Chen
This work reports on a novel method for the determination of total acid (TA) in biomass hydrolysates by a solvent-assisted and reaction-based headspace gas chromatography (HS-GC). The neutralization reaction between the acids in hydrolysates and bicarbonate in an ethanol (50%) aqueous solution was performed in a closed headspace sample vial, from which the carbon dioxide generated from the reaction was detected by HS-GC. It was found that the addition of ethanol can effectively eliminate the precipitation of some organic acids in the biomass hydrolysates. The results showed that the reaction and headspace equilibration can be achieved within 45 min at 70 °C; the method has a good precision (RSD < 3.27%) and accuracy (recovery of 97.4–105%); the limit of quantification is 1.36 μmol. The present method is quite suitable to batch analysis of TA content in hydrolysate for the biorefinery related research.
研究领域:
分析化学
·
生物工程
✓ 已验证
DOI
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2022
·Vol. 23
·期 20
·12086
·
IF 6.208
Jiaojiao Zhang,Shaozhen Wu,Qingqing Wang,Qinjie Yuan,Yane Li,Patricia Reboredo-Rodríguez,Alfonso Varela-López,Zhiping He,Fenghua Wu,Hao Hu,Xingquan Liu
Pecan (Carya cathayensis) is an important economic crop, and its hydrolyzed peptides have been evidenced to reduce the effect of oxidative stress due to their antioxidant capacity. Hence, the protocols of ultrafiltration and gel filtration chromatography were established to obtain bioactive peptides from by-products ofC. cathayensis(pecan cake). As measured by DPPH/ABTS radical scavenging, the peptides with less molecular weight (MW) possess higher antioxidant capacity. PCPH-III (MW < 3 kDa) presented higher radical scavenging capacity than PCPH-II (3 kDa < MW < 10 kDa) and PCPH-I (MW > 10 kDa) measured by DPPH (IC50: 111.0 μg/ mL) and measured by ABTs (IC50: 402.9 μg/mL). The secondary structure and amino acid composition varied by their MW, in which PCPH-II contained more α-helices (26.71%) and β-sheets (36.96%), PCPH-III contained higher ratios of β-turns (36.87%), while the composition of different secondary of PCPH-I was even 25 ± 5.76%. The variation trend of α-helix and random experienced slightly varied from PCPH-I to PCPH-II, while significantly decreased from PCPH-II to PCPH-III. The increasing antioxidant capacity is followed by the content of proline, and PCPH-III had the highest composition (8.03%). With regard to the six peptides identified by LC-MS/MS, two of them (VYGYADK and VLFSNY) showed stronger antioxidant capacity than others. In silico molecular docking demonstrated their combining abilities with a transcription factor Kelch-like ECH-associated protein 1 (Keap1) and speculated that they inhibit oxidative stress through activating the Keap1-Nrf2-ARE pathway. Meanwhile, increased activity of SOD and CAT—antioxidant markers—were found in H2O2-induced cells. The residue of tyrosine was demonstrated to contribute the most antioxidant capacity of VYGYADK and its position affected less. This study provided a novel peptide screening and by-product utilization process that can be applied in natural product developments.Keywords:Chinese pecan by-products;antioxidant peptides;proline content;molecular docking;Keap1-Nrf2-ARE pathway
研究领域:
食品科学
·
生物化学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF GEOPHYSICAL RESEARCH-OCEANS
·
2024
·Vol. 129
·期 11
·e2024JC021414
·
IF 3.3
Junyi Ni,Mingming Chen,Jiaming Shen,Li Liu,Zong-Pei Jiang,Kuanbo Zhou,Xiaolin Li,Minhan Dai,Yao Zhang
The cyclonic eddy uplifts nutrient-rich seawater into the euphotic zone, typically directly enhancing phytoplankton abundance and primary production. However, its impact on heterotrophic prokaryotic production (HPP) remains unclear due to the complex interplay of multiple indirect factors governing this process. Here, we conducted a comprehensive investigation of the distribution of picophytoplankton and heterotrophic prokaryotes, prokaryotic community structure, and HPP within a cyclonic eddy in the western North Pacific subtropical gyre. The results indicated that despite the higher abundance of picophytoplankton accompanied by nutrient upwelling at the eddy core compared to the edge, higher levels of HPP were observed at the eddy edge between 100 and 200 m, consistent with the distribution of the low nucleic acid content (LNA) prokaryotes. The significant positive correlation between HPP and the proportion of LNA group in total heterotrophic prokaryotes suggested a primary contribution from the LNA group over the high nucleic acid content (HNA) group. SAR11, a typical member of the LNA group, may primarily contribute to the elevated HPP observed at the eddy edge. The changes in temperature, nutrients, and light intensity induced by the cyclonic eddy may significantly influence the distribution and activity of HNA and LNA groups, potentially exerting a greater impact on HPP compared to phytoplankton-related factors. These findings contribute to understanding the underlying mechanisms of HPP responses to cyclonic eddies in the oligotrophic open ocean. Key Points Higher heterotrophic prokaryotic production (HPP) occurred at the eddy edge, while picophytoplankton predominantly occurred in the core The elevated HPP at the eddy edge was attributed to low nucleic acid prokaryotes, such as SAR11 The altered temperature, nutrients, and light intensity may impact the distribution and HPP in the eddy Plain Language Summary Mesoscale eddies are common features of global ocean circulation. Cyclonic eddies uplift isopycnals at their centers, bringing cold, nutrient-rich waters from the lower layers to the surface within the eddy, thereby promoting higher phytoplanktonic abundance and primary production typically observed at the eddy core. However, the response of heterotrophic prokaryotic distribution and heterotrophic prokaryotic production (HPP) to the upwelling in the cyclonic eddy remains unclear, as limited studies have not yet reached a consensus. We tracked a cyclonic eddy in the western North Pacific subtropical gyre to compare prokaryotic abundance, community composition, and HPP between eddy core and edge. The results indicate that, while picophytoplankton abundance was higher at the eddy core compared to the edge, HPP was predominantly elevated at the edge in response to the cyclonic eddy. The low nucleic acid content (LNA) prokaryotes dominated by SAR11 may significantly contribute to the increased HPP, as opposed to the traditionally assumed high nucleic acid content (HNA) group. The main factors influencing the distribution of LNA and HNA groups, as well as HPP, include temperature, nutrients, and light intensity altered by the eddy. This study advances the understanding of heterotrophic prokaryotic responses to cyclonic eddy dynamics.
研究领域:
海洋微生物学
·
海洋学
·
生物地球化学
✓ 已验证
DOI
高影响因子