Biotechnology Journal
·
2025
·Vol. 20
·期 3
·e70006
·
IF 3.1
Jingyi Xiao,Zan Ge,Xiaowei Tan,Ziyi Liu,Yafang Zhang,Shufen Xiao,Rongyuan Yi,Ye Hu,Wenyan Hu,Hui Chu,Jian Chen
Traditional beta-cyclodextrin (beta-CD) in biomedical applications faces challenges due to its inherent physical and biochemical limitations. One of the most effective strategies to enhance the properties of beta-CD for drug delivery is the synthesis of supramolecular polycyclodextrins. In this study, we designed a novel beta-CD nanocage-like structure for drug delivery, incorporating imine and disulfide bonds through Schiff base reactions. Aldehyde group-functionalized beta-CD units were used to construct the main backbone of the nanocage, forming dual-dynamic covalent bonds. The chemical structure of the beta-CD nanocage was confirmed using ¹H nuclear magnetic resonance (¹H NMR) and Fourier transform infrared spectroscopy (FTIR). Additionally, atomic force microscopy (AFM) and dynamic light scattering (DLS) revealed that varying amounts of beta-CD crosslinked with cystamine resulted in nanocages approximately 200 nm in size. In vitro drug release experiments demonstrated that doxorubicin (DOX)-loaded beta-CD nanocages exhibited accelerated DOX release in acidic and reductive environments compared to normal physiological conditions, owing to the pH-sensitive imine bond and the glutathione (GSH)-cleavable disulfide bond. The DOX-loaded beta-CD nanocages showed exceptional tumor-killing effects, particularly in acid/reduction-enhanced tumor cells. Both cellular fluorescence imaging and flow cytometry confirmed the potential of the beta-CD nanocages for acid/reduction-specific drug release. Consequently, this precision medicine model using imine/disulfide-linked beta-CD nanocage structures as acidity/reduction-sensitive drug carriers promises to improve oncotherapy through more targeted drug delivery and release, supporting individualized treatment approaches.
研究领域:
药学
·
生物医学工程
·
肿瘤学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2023
·Vol. 11
·期 39
·9525-9531
·
IF 7
Jie Li,Zhuang Ma,Anran Li,Siyuan Huang,Yufei Zhang,Yun Xue,Xianhui Song,Ye Zhang,Shihao Hong,Mo Wang,Zhongming Wu,Xinge Zhang
Microorganism adhesion and the resulting contamination of the biomaterial is one of the major causes of biomedical device failure. Stimuli-responsive materials based on dynamically regulating interactions with reversible characteristics of on–off states have attracted increasing attention. Here, a facile self-assembled biomaterial nanocoating constructed using acidity- and photoregulated spiropyran-modified nanoparticles was developed for reversibly regulating bacteria or mammalian cell adhesion-and-detachment. The coating was formed by coating a solution of spiropyran-conjugated nanoparticles around the surface of a silica gel followed by curing and drying at 60 °C for 30 min. Importantly, efficient adhesion-and-detachment of bacteria or cells could be controlled even after 8 cycles owing to the excellent acidity- and light-switched ability. Collectively, this well-defined self-assembled nanocoating as a dynamical and reversible agent provides promising insight for the development of biomedical devices, especially for biomaterial medical coatings.
研究领域:
生物材料学
·
表面工程
·
微生物学
·
再生医学
·
抗菌技术
✓ 已验证
DOI
高影响因子
CARBOHYDRATE POLYMERS
·
2024
·
IF 10.7
Peiyu Xu,Jing Liu,Ying Yi,Zhi Cai,Yihua Yin,Weiquan Cai,Jingli Zhang,Zhixia Gong,Yaqi Xiao
5-aminolevulinic acid (5-ALA) has been fully demonstrated as a biodegradable, without resistance, and pollution-free pesticide. However, the lack of targeting and the poor adhesion result in a low utilization rate, limiting its practical application. Herein, a dew-responsive polymer pro-pesticide Pec-hyd-ALA was successfully synthesized by grafting 5-ALA onto the pectin (PEC) backbone via acid-sensitive acylhydrazone bonds. When the pro-pesticide is exposed to acid dew on plant surfaces at night, 5-ALA is released and subsequently converted to photosensitize (Protoporphyrin IX, PpIX)in plant cells, leading to its accumulation and promoting photodynamic inactivation (PDI). An inverted fluorescence microscope has verified the accumulation of tetrapyrrole in plant cells. In addition, the highly bio-adhesive PEC backbone effectively improved the wetting and retention of 5-ALA on leaves. The pot experiment also demonstrated the system's control effect on barnyard grass. This work provides a promising approach to improving the herbicidal efficacy of 5-ALA.
研究领域:
农业科学
·
植物生理学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2014
·Vol. 4
·期 93
·51696-51702
·
IF 3.708
Jingwen Zhao,Shichao Jiang,Reila Zheng,Xin Zhao,Xinliang Chen,Cunyi Fan,Wenguo Cui
A smart and stable electrospun fibrous drug carrier was fabricated to release an incorporated drug in response to the local presence of acid and to provide a favourable micro-environment for tissue regeneration. To fabricate the electrospun fibrous scaffolds responsive to the local pH variation, sodium bicarbonate, a component of acid response, was facilely incorporated into drug-loaded electrospun fibers by co-electrospinning. The analysis of X-ray photoelectron spectroscopy, Fourier-transform infrared spectrometry, X-ray diffraction, Differential scanning calorimetry and water contact angles demonstrated that the sodium bicarbonate was successfully incorporated into the electrospun fibers. The in vitro drug release results showed that the release rate of an anti-cancer drug 5-fluorouracil in an acidic environment from the electrospun fibrous scaffolds was proportional to the concentration of sodium bicarbonate, and the structure of the electrospun fibrous scaffolds was well maintained before and after the drug release. The acid-responsive release of 5-fluorouracil for a short time was found to effectively inhibit osteosarcoma cell growth, and the electrospun fibrous scaffolds with long-term stability supported fibroblast adhesion and proliferation, indicating the potential for subsequent tissue regeneration.
研究领域:
生物材料学
·
肿瘤学
·
药剂学
✓ 已验证
DOI
高影响因子
Advanced Optical Materials
·
2024
·Vol. 13
·期 2
·2402038
·
IF 8
Li Yuan,Haiqing Zhu,Wei Zhang,Linxin Yao,Yumiao Su,Wenmu Li
Controllable coordination and efficient sensitization of rare earth ions in polymer materials are key to achieving high-performance optoelectronic polymers. By designing aromatic diamines, polyimides are synthesized with 1,10-phenanthroline benzimidazole groups (PBG) in the polymer backbone. Utilizing the coordination between PBG and rare earth ions (Eu 3 ⁺, Tb 3 ⁺), polyimide-rare earth complexes are formed and precisely characterized their structures. The unique coordination allows PBG to effectively sensitize Eu 3 ⁺, resulting in efficient, long-lived red emission. Furthermore, PBG maintains excellent coordination with Eu 3 ⁺ in acidic environments, granting acid-responsive color changes for data encryption. Importantly, PBG sensitization of Tb 3 ⁺ enabled full-spectrum emission (CIE coordinates: (0.31, 0.35)) within a single rare earth-polyimide complex, due to electron transfer among the ions, ligands, and polymer. This leads to the design of a multi-layer PLED device with an optimal external quantum efficiency of 0.43% for white light emission (CIE coordinates: (0.28, 0.34)). Through comparative theoretical and experimental analysis, the photophysical behavior of these coordinated polyimides is explained and explored their photoluminescent and electroluminescent properties. This research integrates the advantages of rare earth elements and polyimides, creating novel luminescent polymers with diverse optical applications, providing a new strategy for designing luminescent coordination polymers.
研究领域:
材料科学
·
高分子化学
·
光电子学
·
稀土化学
✓ 已验证
DOI
高影响因子
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
·
2014
·
IF 4.245
Zheng Wang,Chao Chen,Qi Zhang,Min Gao,Ju Zhang,Deling Kong,Yanjun Zhao
To precisely manipulate the intracellular delivery of pleiotropic curcumin , this work reports two types of acid-responsive polymer–curcumin conjugates with different structures. One or two amphiphilic poly(ethylene glycol)-co-poly(lactic acid) (PEG-PLA) copolymer(s) were linked to curcumin via pH-liable hydrazone, producing a linear or phospholipid-like conjugate, respectively. Both conjugates efficiently self-assembled into micellar nanocarriers with enhanced stability in contrast to the paralleling PEG-PLA micelles. In comparison to the micelles assembled by phospholipid-like conjugates, the linear conjugate micelles exhibited similar size, doubled loading dose, higher release rate, and hence enhanced cellular uptake and cytotoxicity at the cost of increased critical micelle concentration. This work highlighted the importance of precise tuning of polymer–drug conjugate architecture in determining the pharmaceutical properties and thus delivery efficiency of the corresponding conjugate micelles.
研究领域:
药剂学
·
生物医学工程
·
高分子化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2020
·
IF 6.785
Yanjing Yang,Yao He,Zhiwei Deng,Jiacheng Li,Jin Huang,Shian Zhong
The design of an intelligent nanoprobe capable of intracellular controlled release of apoptosis inducers and subsequent high-fidelity imaging of the drug-induced apoptosis is highly desirable for precise cancer treatment. Herein, we report an intelligent nanoprobe that combined therapeutic and imaging functions in one agent. Briefly, a gold nanoparticle is designed to be conjugated with acid-responsive DNA duplexes (Dox intercalates in this region) and caspase-3-specific cleavable peptides (labeled with fluorophore). We demonstrated that the nanoprobe could efficiently deliver an anticancer drug (Dox) into cancer cells and achieve acid-responsive drug release. Furthermore, the apoptotic process was in situ-monitored by detection of fluorescence through the cleavage of the peptide linker by caspase-3, which is one of the executioner caspases involved in apoptosis. This newly developed nanoprobe could serve as a theranostic agent for targeted responsive chemotherapy and also provide feedback apoptosis imaging of the self-therapeutic effect.
研究领域:
生物医学工程
·
分子生物学
·
纳米医学
✓ 已验证
DOI
PubMed
高影响因子
MACROMOLECULAR BIOSCIENCE
·
2016
·Vol. 17
·期 3
·1600227
·
IF 3.68
Chunran Wang,Jinze Wang,Xiaofei Chen,Xu Zheng,Zhigang Xie,Li Chen,Xuesi Chen
Herein, a kind of dual acid-sensitive nanoparticles based on monomethoxy poly(ethylene glycol)-imine-β-cyclodextrin is constructed by a facile phenylboronic acid-cross-linked way. The data of dynamic light scattering and transmission electron microscope reveal the cross-linked nanoparticles have improved stability. The cross-linked nanoparticles could easily self-assemble and load the anticancer drug at neutral pH condition. However, when the drug-loaded nanoparticles are delivered to extracellular tumor sites (pH ≈6.8), the surface of the nanoparticles would be amino positively charged and easily internalized by tumor cell due to the cleavage of the acid-labile benzoic–imine. Subsequently, with the acidity in subcellular compartments significantly increasing (such as the endosome pH ≈5.3), the loaded drug would fast release from the endocytosis carriers due to the hydrolysis of boronate ester. These features suggest that these dual acid-sensitive cross-linked nanoparticles not only possess excellent biocompatibility but also can efficiently load and deliver anticancer drug into tumor cells to enhance the inhibition of cellular proliferation, outlining a favorable platform as drug carriers.
研究领域:
药剂学
·
肿瘤学
·
生物材料学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2023
·
IF 5.2
Hailiang Chen,Chenyu Liu,Simiao Yu,Hengjun Zhou,Farishta Shafiq,Weihong Qiao
The emergence of liposomes has provided a breakthrough for the clinical use of highly toxic chemotherapeutic drugs . However, the clinical effects of conventional drug-loaded liposomes are unsatisfactory due to the lack of specific targeting of cancer cells and the inability to achieve endosome escape and controllable release in vivo. In this paper, a cRGD-PEOz-Hz-DOB (cPzD) molecule with specific targeting, endosomal escape, and endogenous acid-sensitive controlled release ability was creatively designed and synthesized to prepare drug-loaded liposomes cPzDGX encapsulating the anticancer drug Doxorubicin (DOX) in order to enhance the anticancer performance of DOX. The study found that the hydrazone bond in cPzD reached a breakage rate of 42% within 48 h at pH 5.0, so the cumulative release rate of cPzDGX at pH 5.0 for 48 h was as high as 82.9%, which was 22.5% higher than that at pH 7.4. Moreover, the uptake of cPzDGX in A549/ADR cells was increased by 62% at 2 h, and the expression of the green fluorescent protein gene EGFP was also increased by 36% relative to the targeting control group, indicating that cRGD modification could effectively improve the targeting ability of liposomes to cancer cells. In addition, it was demonstrated that PEOz modification could help drug carriers achieve endosome escape because the green fluorescent protein expression in the PEOz-modified liposome group was 60.5% higher than that in the PEG-modified group. Finally, the cell viability of the cPzDGX group decreased by 17.2% relative to the free DOX group when the DOX concentration was 1 μg/mL in the cytotoxicity assay, and the diameter reduction of 3D tumor MCs increased by 34.4%. And cPzDGX could effectively enhance the permeability of free DOX to solid tumors. The above results indicate that the DOX-loaded liposomes cPzDGX we designed have great prospects for the accurate and efficient treatment of cancer.
研究领域:
肿瘤学
·
药剂学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2025
·
IF 2.5
Zhiting Yang,Jiahua Tang,Yan Zhang,Xianzhi Hu,Futing Zi,Yue Lin,Jin Wu
Acid mine drainage (AMD) is a persistent and challenging environmental pollution problem. The consequences of AMD include soil pollution, biodiversity loss, and ecosystem collapse. Our study found that rhodamine B acylhydrazone (RBA) exhibits high sensitivity to Fe3+ and can be used as a corrosion inhibitor. This finding led us to hypothesize that the application of RBA is an effective strategy for constructing self-warning and self-healing integrated pH-responsive mercaptopropyl trimethoxysilane (PeopS-SH) coatings. We herein successfully prepared RBA-loaded mesoporous silica nanoparticles (MSN@RBA) designed to exhibit localized, acid-responsive behavior to avoid oxidation site diffusion and enhance wound healing. These MSN@RBA were sensitive to acidic environments, triggering the rapid release of RBA. The experiments showed that MSN@RBA effectively inhibited the oxidation rate of pyrite and enhanced the long-term passivation effect of the coating. Our findings further demonstrated that MSN@RBA reduced the corrosion current density (0.17 μA cm−2), increased the corrosion potential (335 mV), and improved the thickness and density of the coating. The results of release experiments, electrochemical tests and chemical leaching indicate that MSN@RBA releases RBA in acidic environments, and its spironolactam structure opens and forms a complex with Fe3+. The damaged site is fluorescently labeled and repaired. In conclusion, this acid-responsive nanoparticle system presents a new strategy for the treatment of tailings in harsh environments for a long time.
研究领域:
材料科学
·
环境科学
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
ORGANIC & BIOMOLECULAR CHEMISTRY
·
2013
·Vol. 11
·期 45
·7931-7937
·
IF 3.568
Xinhua Cao,Aiping Gao,Haiting Lv,Yan Wu,Xiuxiu Wang,Yang Fan
A new gelator 1 that included m-methyl red was synthesized and fully characterized. It was found that the organogel of 1 was stable in DMSO even if the organogel was stimulated by acid or light. The organogel was thoroughly characterized using various microscopic techniques including field-emission scanning electron microscopy (FESEM), X-ray diffraction (XRD), UV-vis and Fourier transform infrared (FTIR) spectra. The organogel exhibited tunable structures and optical properties under different stimulation. The regular nanoring structure was turned into microspheres after the organogel in DMSO was stimulated at 254 nm light or acid. At the same time, the color of molecule 1 in gel state and solution state was all changed after stimulation by light or acid. The hydrogen bonding and π–π stacking were found to be the main driving forces for gel formation. This dual-responsive gel held promise for soft materials application in upscale smart responsive materials.
研究领域:
材料化学
·
软物质科学
✓ 已验证
DOI
PubMed
高影响因子
MACROMOLECULAR BIOSCIENCE
·
2016
·Vol. 17
·期 3
·1600258
·
IF 3.68
Shan Zhang,Jianbin Xu,Heng Chen,Zhangfa Song,Yalan Wu,Xingyi Dai,Jie Kong
In this contribution, amphiphilic star copolymers (H40- star -PCL- a -PEG) with an H40 hyperbranched polyester core and poly(ε-caprolactone)- a -poly(ethylene glycol) copolymer arms linked with acetal groups are synthesized using ring-opening polymerization and a copper (I)-catalyzed alkyne-azide cycloaddition click reaction. The acid-cleavable acetal groups between the hydrophilic and hydrophobic segments of the arms endow the amphiphilic star copolymers with pH responsiveness. In aqueous solution, unimolecular micelles can be formed with good stability and a unique acid degradability, as is desirable for anticancer drug carriers. For the model drug of doxorubicin, the in vitro release behavior, intracellular release, and inhibition of proliferation of HeLa cells show that the acid-cleavable unimolecular micelles with anticancer activity can be dissociated in an acidic environment and efficiently internalized by HeLa cells. Due to the acid-cleavable and biodegradable nature, unimolecular micelles from amphiphilic star copolymers are promising for applications in intracellular drug delivery for cancer chemotherapy.
研究领域:
材料科学
·
药学
·
肿瘤学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）