SEPARATION AND PURIFICATION TECHNOLOGY
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2022
·
IF 9.136
Chengzhen Zhu,Junhua Li,Junbin Liao,Quan Chen,Yanqing Xu,Huimin Ruan,Jiangnan Shen
Electrodialysis (ED) technology equipped with proton blocking anion exchange membranes (AEMs) has great potential applications in recovering acidic wastewater. To develop high performance proton-blocking AEMs, in this work, a series of imidazolium functionalized poly(vinyl chloride) (PVC) AEMs are designed. In particular, the length of alkyl side chains tethered on the imidazole compound is optimized to adjust the micro-structure of AEM. Our research demonstrates that the density of 1-butylimidazole functionalized PVC AEM matrix is enhanced, which has been proved by water uptake and thermogravimetric analysis . Hence, the acid blocking performance of optimized AEM (PVC-Im-4C) is strengthened. The maximum concentration of H + in concentrate chamber of electrodialyser enriched by the PVC-Im-4C AEM is 1.81 M through the 24 h ED process at a current density of 20 mA·cm −2 (the initial concentration of hydrogen ions: 1.0 M). The result suggests that the optimized PVC-Im-4C AEM with long alkyl side chain on imidazole shows the excellent capacity in acid enrichment. This work should provide guidance for the follow-up design of the advanced proton blocking AEMs.
研究领域:
材料科学
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环境工程
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DOI
高影响因子
JOURNAL OF MEMBRANE SCIENCE
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2020
·
IF 7.183
Junbin Liao,Huimin Ruan,Xing Gao,Quan Chen,Jiangnan Shen
The use of electrodialysis (ED) fabricated with proton blocking anion exchange membranes (AEMs) is a promising method to realize waste acid reclamation. To develop AEMs with low proton leakage, in this work, we designed a series of N -methylpiperidine (MPRD)-functionalized poly(2,6-dimethyl-1,4-phenylene oxide) (PPO) cross-linked by weak base 1,3-di-4-piperidylpropane (DiPRD). Our investigations demonstrate the weak base cross-linker could enhance the density of membrane matrix and reduce the hydrophilicity of AEM matrix, which have been verified by thermogravimetric analysis and water absorption with low swelling ratio of 2.43%. Benefit from the unique structure with denser membrane matrix and additional protonated weak base piperidine structure, the acid blocking performance of the optimized crosslinked AEM was enhanced with the H + concentration of 1.98 M in concentrate cell during 24 h-ED process at current density of 20 mA cm −2 (initial H + concentration: 1.0 M), outperforming the AEM without cross-linking structure (1.73 M). Nevertheless, it was found that the small-sized proton diffusion in ion-nanochannels of AEM and the aged membrane matrix in ED process with high sulfuric acid concentration resulted in proton leakage to some degree. As a result, a trade-off between the proton blocking and sulfate ion transport of the cross-linked AEMs is achieved. It is believed that this study can provide a guideline for designing advanced proton blocking AEMs.
研究领域:
化学工程
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材料科学
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DOI
高影响因子
MOLECULAR PHARMACEUTICS
·
2016
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IF 4.342
Qian Jiang,Dong Yue,Yu Nie,Xianghui Xu,Yiyan He,Shiyong Zhang,Ernst Wagner,Zhongwei Gu
Cationic lipid based assemblies provide a promising platform for effective gene condensation into nanosized particles, and the peripheral properties of the assemblies are vital for complexation and interaction with physical barriers. Here, we report three cationic twin head lipids, and each of them contains a dioleoyl-glutamate hydrophobic tail and a twin polar head of lysine, arginine, or histidine. Such lipids were proven to self-assemble in aqueous solution with well-defined nanostructures and residual amino-, guanidine-, or imidazole-rich periphery, showing strong buffering capacity and good liquidity. The assemblies with arginine (RL) or lysine (KL) periphery exhibited positive charges (∼+35 mV) and complete condensation of pDNA into nanosized complexes (∼120 nm). In contrast, assemblies composed of histidine-rich lipids (HL) showed relatively low cationic electric potential (∼+10 mV) and poor DNA binding ability. As expected, the designed RL assemblies with guanidine-rich periphery enhanced the in vitro gene transfection up to 190-fold as compared with the golden standard PEI25k and Lipofectamine 2000, especially in the presence of serum. Meanwhile, interaction with cell and endo/lysosome membrane also revealed the superiority of RL complexes, that the guanidine-rich surface efficiently promoted transmembrane process in cellular internalization and endosomal disruption. More importantly, RL complexes also succeeded beyond others in vivo with significantly (∼7-fold) enhanced expression in HepG2 tumor xenografts in mice, as well as stronger green fluorescence protein imaging in isolated tumors and tumor frozen sections.
研究领域:
分子生物学
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基因治疗
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纳米医学
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DOI
PubMed
高影响因子
PEST MANAGEMENT SCIENCE
·
2020
·Vol. 77
·期 4
·2129-2141
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IF 3.75
Li-Sha Bai,Jing-Jing Xu,Cai-Xia Zhao,Ya-Li Chang,Yan-Ling Dong,Kai-Ge Zhang,Yong-Qiang Li,Yi-Ping Li,Zhi-Qing Ma,Xi-Li Liu
BACKGROUND Carboxylesterase (CarE) is a major class of enzyme involved in the detoxification of toxic xenobiotics in various insect species. Previous work has shown that the carboxylesterase gene CarE001G found in Helicoverpa armigera is more active and can metabolize synthesized pyrethroids, such as β -cypermethrin, one of the commonly used commercial insecticides for lepidopteran pest control. In addition, CarE001G is very special as it has a very specific glycine-rich region located adjacent to its C-terminal. But whether mutations in this unique sequence can change the biochemistry and function of CarE001G are unknown. RESULTS In this study, four variants of CarE001G with different deletions in the glycine-rich region were obtained and functionally expressed in Escherichia coli . The recombinant proteins were purified and confirmed by Western blot and mass spectrometry analyses. These mutant enzymes showed high catalytic efficiency toward the model substrate α -naphthyl acetate. Inhibition study showed that β -cypermethrin had relatively strong inhibition on CarE activities. In vitro metabolism assay showed that the mutant enzymes significantly enhanced their metabolic activities toward β -cypermethrin with specific activities between 4.0 and 5.6 nmol L -1 min −1 mg −1 protein. Molecular docking analyses consistently demonstrated that deletion mutations in the glycine-rich region may facilitate the anchoring of the β -cypermethrin molecule in the active binding pocket of the mutant enzymes. CONCLUSION The data show that deletion mutations can cause qualitative change in the capacity of CarEs in the detoxification of β -cypermethrin. This indicates that deletion mutations in the glycine-rich region may have the potential to cause synthesized pyrethroid (SP) resistance in H. armigera in the future. © 2020 Society of Chemical Industry
研究领域:
昆虫学
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分子生物学
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农药毒理学
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DOI
PubMed
高影响因子
Gels
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2025
·Vol. 11
·期 12
·1007
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IF 5.3
Mengting Fan,Beizhe Huang,Yuhan Li,Ting Zhang,Ranjith Kumar Kankala,Jianting Zhang
Protein-based hydrogels have emerged as an important class of materials for biomedical applications. However, these hydrogels often exhibit inferior mechanical properties, significantly limiting their potential applicability. Herein, a simplified yet efficient soaking method is developed to broaden the scope of constructing stiff and tough pure protein hydrogels, using natural cysteine-rich proteins, such as lactoferrin (LF). The preformed, soft and brittle, unfolded protein hydrogels transformed into highly stretchable and compressible elastomers after soaking in H2O2due to chain entanglement and self-crosslinking via interchain disulfide bonds. As a result, the H2O2-treated LF hydrogels exhibited an extraordinary ultimate strength (compressive and tensile strains of over 90% and 400%, respectively, and stresses of 20 and 1.5 MPa). In addition, these rubber-like hydrogels exhibited exceptional self-recovery and fatigue resistance capabilities. Furthermore, the relationship between protein structure and the mechanical properties of the hydrogel was investigated. Together, these revelations could serve as a guiding principle for advancing the design of biocompatible, tough protein hydrogels without chemical modification or mechanical reinforcing fillers.
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DOI
PubMed
高影响因子
Science Advances
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2019
·Vol. 5
·期 6
·
IF 12.804
Chongyi Liu,Tao Wan,Hui Wang,Song Zhang,Yuan Ping,Yiyun Cheng
Cytosolic protein delivery is of central importance for the development of protein-based biotechnologies and therapeutics; however, efficient intracellular delivery of native proteins remains a challenge. Here, we reported a boronic acid–rich dendrimer with unprecedented efficiency for cytosolic delivery of native proteins. The dendrimer could bind with both negatively and positively charged proteins and efficiently delivered 13 cargo proteins into the cytosol of living cells. All the delivered proteins kept their bioactivities after cytosolic delivery. The dendrimer ensures efficient intracellular delivery of Cas9 protein into various cell lines and showed high efficiency in CRISPR-Cas9 genome editing. The rationally designed boronic acid–rich dendrimer permits the development of an efficient platform with high generality for the delivery of native proteins.
研究领域:
生物技术
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药学
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分子生物学
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基因组编辑
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DOI
PubMed
高影响因子
ONCOLOGY REPORTS
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2025
·Vol. 53
·期 3
·1-14
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IF 3.9
Dongsheng Lv,Xu Han,Liang Hao,Zhimin Sun,Aobo Zhang,Jing Liu,Liang Liu,Liqiang Liu
Cysteine‑ and glycine‑rich protein 2 (CSRP2) are closely associated with tumor invasion and metastasis. CSRP2 is significantly upregulated in glioma tissues and is associated with the clinical stage of the tumor. Overexpression of CSRP2 in glioma cells promotes the proliferation and metastasis of cancer cells, whereas CSRP2 knockdown inhibits the biological functions of tumor cells. Transcriptome sequencing of CSRP2‑knockdown U251M cells revealed that silencing of CSRP2 inhibited the JAK‑STAT1 signaling pathway, and differentially expressed genes were significantly enriched in cell processes related to necroptosis. Experiments on necroptosis in glioma cells using flow cytometry, Hoechst 33342/PI dual staining and transmission electron microscopy indicated that CSRP2 overexpression inhibited necroptosis in glioma cells. Western blotting results showed that overexpression of CSRP2 activated the JAK‑STAT1 signaling pathway, while the addition of the pathway inhibitor ruxolitinib promoted the phosphorylation of necroptosis proteins RIPK1, RIPK3 and MLKL. Therefore, it was hypothesized that CSRP2 maintains JAK‑STAT1 activation by inhibiting the protein inhibitor of activated STAT1, which then inhibits the necrotizing apoptosis of glioma cells.
研究领域:
肿瘤学
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细胞生物学
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分子生物学
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神经肿瘤学
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DOI
PubMed
高影响因子
ACS Sustainable Chemistry & Engineering
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2020
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IF 7.632
Shuo Shi,Wentao Zhang,Haofen Wu,Yuchen Li,Xinyi Ren,Min Li,Jie Liu,Jing Sun,Tianli Yue,Jianlong Wang
Selective enrichment and purification of histidine (His)-rich protein from complex mixtures is vital to satisfy the requirements of proteomic and diagnostic applications, but it still remain a challenge. Herein, a high-performance layered double hydroxide (LDH)-based adsorbent is designed and prepared, via a metal–organic framework (MOF)-mediated in situ cascade derivation strategy, for enhanced His-rich protein separation. Importantly, the pseudomorphic conversion of Fe3O4 nanoparticle-derived magnetic MIL-100(Fe) MOF, with unique structural features, enables the finally derived LDH with plenty of Ni2+ ions exposed for His-rich protein binding. As such, the as-prepared Fe3O4@NiFe LDH exhibits favorable selectivity for His-rich protein adsorption with bovine hemoglobin (BHb) as a model and with a relatively high binding capacity of 3287.67 mg g–1 in a rapid equilibrium time of 20 min, which is superior to those reported magnetic adsorbents. Also, the high performance of Fe3O4@NiFe LDH can be facilely regenerated and applied to separate BHb from a bovine blood sample, highlighting its good practicality. Therefore, this MOF-mediated cascade derivation strategy is a promising way to design advanced layered double hydroxide magnetic adsorbents for high-performance bioseparation.
研究领域:
材料科学
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生物化学
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分析化学
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纳米技术
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DOI
高影响因子
International Journal of Nanomedicine
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2016
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IF 4.32
ChunYan Li,ZhiGang Huang,ZheShuo Liu,LiQian Ci,ZhePeng Liu,Yu Liu,XueYing Yan,WeiYue Lu
Effective interaction between mucoadhesive drug delivery systems and mucin is the basis of effective local placement of drugs to play its therapeutic role after mucosal administration including vaginal use, which especially requires prolonged drug presence for the treatment of gynecological infectious diseases. Our previous report on phenylboronic acid-rich nanoparticles (PBNPs) demonstrated their strong interaction with mucin and mucin-sensitive release profiles of the model protein therapeutics interferon (IFN) in vitro, but their poor stability and obvious tendency to aggregate over time severely limited future application. In this study, sulfonate-modified PBNPs (PBNP-S) were designed as a stable mucoadhesive drug delivery system where the negative charges conferred by sulfonate groups prevented aggregation of nanoparticles and the phenylboronic acid groups ensured effective interaction with mucin over a wide pH range. Results suggested that PBNP-S were of spherical morphology with narrow size distribution (123.5 nm, polydispersity index 0.050), good stability over a wide pH range and 3-month storage and considerable in vitro mucoadhesion capability at vaginal pH as shown by mucin adsorption determination. IFN could be loaded to PBNP-S by physical adsorption with high encapsulation efficiency and released in a mucin-dependent manner in vitro. In vivo near-infrared fluorescent whole animal imaging and quantitative vaginal lavage followed by enzyme-linked immunosorbent assay (ELISA) assay of IFN demonstrated that PBNP-S could stay in the vagina and maintain intravaginal IFN level for much longer time than IFN solution (24 hours vs several hours) without obvious histological irritation to vaginal mucosa after vaginal administration to mice. In summary, good stability, easy loading and controllable release of protein therapeutics, in vitro and in vivo mucoadhesive properties and local safety of PBNP-S suggested it as a promising nanoscale mucoadhesive drug delivery system for vaginal administration of protein therapeutics.
研究领域:
药剂学
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药物递送
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生物医学工程
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妇科学
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产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2023
·
IF 6.2
Ke Yang,Jingyu Pan,Guoqing Deng,Changyi Hua,Cancan Zhu,Yong Liu,Ling Zhu
Mobile sensing enabled by MS 2 technology, which integrates microfluidic and smartphone components, has seen many applications in recent years. In this direction, we developed an MS 2 platform (an integrated kit) for nucleic acid assay, which included a chitosan-modified minimalistic microfluidic chip (CM 3 -chip), a smartphone-based fluorescence detector (SF-detector), an APP for imaging and analysis, reagents, and accessories. Once the lysed sample was loaded into the CM 3 -chip modified by 1% concentration and 200–260 kDa molecular weight of chitosan, the following assay can be completed in approximately 1 h. The Mkit can detect 3 × 10° copies μL −1 of plasmid DNA and its polymerase chain reaction (PCR) efficiency was 96.8%. The CM 3 -chip equipped for the Mkit can enrich nucleic acid from the pH = 5 of lysis buffer, instead of using conventional adsorption mediums such as the magnetic beads and silica gel membranes, which could result in unexpected impurity residuals and tedious cleaning operations. In addition, the performance of the Mkit equipped with the pristine chip was demonstrated to perform poorer than that coupled with the CM 3 -chip in which the enriched nucleic acid can be all used for “ in-situ PCR”. The universality, selectivity, and user-friendliness of the Mkit were also validated. We finally demonstrated the feasibility of the Mkit for testing artificially prepared infected samples. H5N6 and IAV-infected saliva samples provided the limits of detection of 5 × 10 2 copies mL −1 and 3.24 × 10 2 copies mL −1 per chamber, respectively. The streamlined assay and compact device should enable the great potential of the Mkit in research and potential diagnostic uses.
研究领域:
生物技术
·
微流控
·
分子生物学
·
即时诊断
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2023
·
IF 4.5
Yong Chang,Meiling Liu,Tong Wu,Ruting Lin,Lin Liu,Qijun Song
Most of proteins in the commercial ELISA kits are recombinant with hexahistidine (His 6 ) tags. Based on this fact, we proposed a simple procedure for the preparation of signal labels and the design of competitive immunosensors. The signal labels were fabricated through the high-affinity interaction between the His 6 tails on the surface of recombinant proteins and the coordinatively unsaturated copper ions on the surface of pristine Cu-based metal organic framework (Cu-MOF). The recombinant protein-modified MOF could be captured by the sensor electrode, thus producing a strong differential pulse voltammetry signal through the electrochemical reduction of Cu 2+ ions in MOF. However, the target protein in sample solution could compete with the His 6 -tagged protein on the surface of Cu-MOF to bind the antibody attached on the electrode, thus leading to the decrease in the electrochemical signal. The peak current showed an inversely relationship with the concentration of target protein. The competitive immunosensor exhibited a linear range of 1 pg/mL to 1 ng/mL with SARS-CoV-2 nucleocapsid protein ( N -protein) as the target analyte. We believe that the method can be used to detect other proteins by utilizing the characteristic of recombinant proteins in commercial kits.
研究领域:
分析化学
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生物化学
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免疫学
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电化学
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DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Fei Qu,Zhuo Wang,Cong Li,Dafeng Jiang,Xian-en Zhao
Herein, a novel peptidyl probe is first synthesized by the amide reaction between glutathione-capped gold nanoclusters (GSH-AuNCs) and positive charged arginine-rich peptide (Arg 9 ), and it is called Arg 9 -GSH-AuNCs. Polyuridylic acid (polyU), as a polyanion , is firstly used to induce the aggregation-induced emission enhancement (AEE) of positive charged Arg 9 -GSH-AuNCs through electrostatic attraction . The peptide bond on the carboxyl terminal of the arginine residues can be recognized and cleaved by trypsin. Hence, polyU is hard to induce the aggregation of peptidyl probe again, and the AEE from GSH-AuNCs becomes weak. It is an ideal platform to detect trypsin and screen trypsin inhibitors . The fluorescent peptidyl probe exhibits excellent sensitivity for trypsin. A good linear range of trypsin is obtained from 1 to 50 ng·mL −1 with the limit of detection (LOD) 0.31 ng·mL −1 , which is lower than those of most reported works. Furthermore, the inhibitory effects of the extracts from foods (i.e., soybean seed flour, black soybean seed flour, mung bean seed flour, and azuki bean seed flour) on the trypsin activity have been studied. The half maximal inhibitory concentration (IC 50 ) values of extracts from soybean seed flour, black soybean seed flour, mung bean seed flour, and azuki bean seed flour are 4.5, 9.3, 11.6, 20.9 μg·mL −1 , respectively. The screening results show that these extracts from foods all have inhibitory effects on trypsin. Among them, the extract from soybean seed flour exhibits significant inhibitory effect.
研究领域:
分析化学
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生物化学
·
食品科学
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DOI
高影响因子
危险品化学品经营许可证（带存储）