INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2017
·
IF 3.671
Huiling Liu,Yueming Jiang,Hongshun Yang,Bao Yang
Banana is one of the most important fruits over the world. The chemical composition is critical for the organoleptic properties and health benefits. As one of the leading bioactive components in banana pulp, the polysaccharides may contribute to the beneficial health effects. However, their precise structure information remains unknown. A leading acidic polysaccharide (ABPP) of banana pulp was purified and identified by nuclear magnetic resonnance spectroscopy (NMR) and gas chromatography-mass spectrometry (GC–MS). →4-α- d -Gal p A-1→ and →4-α- d -Gal p AMe-1→ constituted the backbone. No branch chains were detected. The molecular weight was determined to be 8.9 kDa by gel permeation chromatography, which was smaller than previously reported fruit-derived polygalacturonic acids. The precise structure was identified as below. Digestion by enzyme would lead to production of oligogalacturonic acids and quick accumulation of 5000–7000 Da fraction. Download : Download high-res image (52KB) Download : Download full-size image
研究领域:
食品科学
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植物化学
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DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
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2025
·
IF 6.2
Baojie Zhu,Wenxia Zhang,Shaoping Li,Jing Zhao
The polysaccharides from L. barbarum L. fruits (LBPs) are a kind of complex polysaccharides, which mainly contribute to the bioactivity of L. barbarum L. fruits and riches in acidic polysaccharides fractions. And the pharmacological research showed that the immunomodulatory effect of LBPs was closely related to α -1,4-D-galactosiduronic linkages. Therefore, as one of the main active polysaccharide fractions in LBPs, acidic polysaccharides fraction should also be used for the quality control of L. barbarum L. fruits. In this study, a novel quantitative evaluation strategy of polysaccharides from L. barbarum L. fruits based on simultaneous quantification of total polysaccharides and polysaccharides with active structure characteristics was proposed to improve their performance in the medicinal and functional food area more comprehensively and effectively, and beneficial to improve the quality control of L. barbarum L. fruits. The content of total polysaccharides in L. barbarum L. fruits was determined by HPSEC-RID method combined with universal dn / dc (0.15 mL·g −1 ). Additionally, HPAEC-PAD method combined with pectinase hydrolysis and m-hydroxybiphenyl colorimetric method were developed for the determination of acidic polysaccharide components related to active structural characteristics in L. barbarum fruits. The results revealed that the trends of total polysaccharides and acidic polysaccharides in L. barbarum L. fruits did not entirely coincide, further emphasizing the necessity of simultaneously quantifying both fractions for quality control. Furthermore, the m-hydroxybiphenyl method can be used as an alternative method to determine the content of acidic polysaccharides by HPAEC-PAD combined with pectinase hydrolysis.
研究领域:
药学
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食品科学
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植物化学
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高影响因子
GLYCOCONJUGATE JOURNAL
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2017
·Vol. 34
·期 5
·625-632
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IF 2.186
Song Shuang,Yu Qi,Zhang Bao,Ai Chunqing,Sun Yujiao,Fu Yinghuan,Zhao Meiyu,Wen Chengrong
Fish intestines and livers are usually considered as delicious and nutritious food in China. Acidic polysaccharides are important nutrients in these food of animal origin, but there is currently little information regarding their quantitative distributions. The present study demonstrated a method to quantify acidic polysaccharides simultaneously by analyzing their disaccharides produced from the acid hydrolysis using high-performance liquid chromatography (HPLC) coupled with triple quadrupole mass spectrometry. The recoveries for these acidic polysaccharides were all 97%–115% with relative standard deviation of 3.0%–9.0%. All of the acidic polysaccharides had good linearities. Then this method was applied to determine the composition of acidic polysaccharides in 5 edible fish livers and intestines. Besides well-known glycosaminoglycans (GAGs) including hyaluronic acid (HA), Chondroitin sulfate (CS), dermatan sulfate (DS) and heparin (HP), 4 novel acidic polysaccharides including 2 GAGs and 2 non-GAGs comprised of hexose-hexuronic acid repeating units were also found. CS and HP were the major acidic polysaccharides components in fish intestines and livers, respectively. The absolute amounts of acidic polysaccharides differed greatly in these fish tissues, but their proportions showed similarity in the same type of tissues. The present study demonstrated an effective method for acidic polysaccharides quantification, and revealed acidic polysaccharides compositions of edible fish livers and intestines.
研究领域:
食品科学
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分析化学
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营养学
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DOI
PubMed
高影响因子
Journal of Functional Foods
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2021
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IF 4.451
Xizhu Wang,Liyan Yuan,Zhijie Bao,Baoshang Fu,Pengfei Jiang,Tiecheng Ma,Songyi Lin
To screen the in vitro uric acid-lowering activity of the neutral polysaccharides (NCSP) and acid polysaccharides (ACSP) isolated from the corn silk were evaluated. Furthermore, preliminary structure-activity relationships for them were investigated. The molecular weight (Mw) of NCSP and ACSP were 74.1 and 109.4 kDa, respectively. NCSP consisted of mannose (Man), glucuronic acid (GlcA), glucose (Glc), galactose (Gal), and arabinose (Ara), in addition to these monosaccharides, the ACSP contains galactose acid (GalA). NMR spectroscopy showed that the NCSP main chain consisted of 1,4,6)- α -D-Glc p , 1,4)- α -D-Glc p , 1,3)- β -D-Gal p , and 1,3,5)- β -D-Man p . NCSP exhibited higher activity in anti-hyperuricemia (HUA). The results indicated that serum uric acid (UA) and liver xanthine oxidase (XO) activities levels decreased by 45.71% and 22.4% in the NCSP groups, respectively. In addition, NCSP could significantly improve the kidney damage and promote uric acid excretion. These findings provide evidence in support of NCSP as a potential dietary supplement for improving HUA.
研究领域:
药理学
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生物化学
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营养学
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DOI
高影响因子
JOURNAL OF FOOD SCIENCE
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2025
·Vol. 90
·期 1
·e70015
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IF 3.4
Jingxuan Yang,Yi Wu,Ziye Jiang,Lili Jiao,Yang Wang
This study aimed to investigate the potential hypoglycemic mechanism of red ginseng acidic polysaccharides (RGAP) from the perspective of fatty acid (FA) regulation. A high-glucose/high-fat diet in conjunction with streptozotocin administration was employed to establish type 2 diabetes mellitus (T2DM) rat models, and their fecal FAs were detected using the liquid chromatography-mass spectrometry (LC-MS) method. RGAP treatment alleviated the polyphagia, polydipsia, weight loss, and hyperglycemia observed in T2DM rats. FA profile was disturbed by T2DM modeling, and 11 marker FAs were selected from statistical analysis, whose intensities were reversely changed by RGAP administration. Among these marker FAs, short-chain FAs were negatively correlated with the fasting blood glucose (FBG) level, while positive correlations were observed between long-chain FA and the FBG level. Combined with the metabolite-enzyme-gene network analysis, we inferred that the mechanistic mechanism RGAP on T2DM may be associated with the regulation of FA metabolism and inflammation-related signaling pathways. This study confirmed the regulatory effect of RGAP on fecal FA, which can provide a scientific basis and new ideas for developing red ginseng as a functional food for supplementary treatment of T2DM.
研究领域:
营养学
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代谢组学
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药理学
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DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
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2019
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IF 5.839
Huifang Shi,Yujun Wan,Ouye Li,Xiang Zhang,Mingyong Xie,Shaoping Nie,Junyi Yin
Monosaccharide composition analysis is important to characterize the structure of complex carbohydrates . However, techniques for analyzing this in acidic polysaccharides are immature, due to incomplete hydrolysis . The aim of this paper is to provide an improved method for this using two-step acid hydrolysis combined with high-performance anion exchange chromatography . A weak acid was used to hydrolyze polysaccharides before hydrolyzing them with a strong acid to dissociate more monosaccharides. In the first step, samples were hydrolyzed using 0.09 M trifluoroacetic acid at 79 °C for 1.5 h to cleave some glycosidic bonds. After TFA removal, hydrolysis was continued at 100 °C for 2 h with 2 M sulfuric acid. The validity of this method was verified for acidic polysaccharides (including standard and three acidic polysaccharides from natural plants), and the total monosaccharides of all samples detected by the two-step acid hydrolysis method was significantly increased comparing to sulfuric acid hydrolysis alone. It suggests that this hydrolysis method could be applied to the monosaccharide composition analysis for acidic polysaccharides.
研究领域:
分析化学
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碳水化合物化学
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生物化学
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DOI
高影响因子
Food & Function
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2018
·Vol. 9
·期 7
·3974-3985
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IF 3.289
Lei Wang,Chun Chen,Bin Zhang,Qiang Huang,Xiong Fu,Chao Li
A novel acidic polysaccharide (RTFP-3) was isolated and purified from Rosa roxburghii Tratt fruits. Structural analyses revealed that RTFP-3 is a homogeneous polysaccharide with a molecular weight of 67.2 kDa and mainly consists of arabinose (37.20%), galactose (34.40%), glucose (10.02%) and fucose (18.30%). The structural chain of RTFP-3 was proven to be composed of →6)-α-D-Galp-(1→, →4)-α-D-GalpA-(1→, →5)-α-L-Araf-(1→, →4)-β-D-Glcp-(1→, β-D-Glcp-(1→ and →3,4)-β-L-Fucp-(1→ residues by periodate oxidation–Smith degradation, methylation, and 1D- and 2D-NMR analyses. Bioassay results showed that RTFP-3 exerted favorable mixed type inhibitory activities against α-glucosidase, and its half inhibitory concentration (IC50) value was determined as 4.15 mg mL−1. RTFP-3 showed static type quenching of the intrinsic fluorescence of α-glucosidase, during which the binding process induced the conformational change of α-glucosidase. These results suggest that RTFP-3 has the potential to be a novel α-glucosidase inhibitory agent for use in functional foods.
研究领域:
食品科学
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植物化学
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生物化学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
·
IF 7.7
Kui Zhao,Cheng Qian,Luming Qi,Qing Li,Can Zhao,Jing Zhang,Guiqi Han,Lina Xia,Zeinhom M. El-Bahy,Junwei Gu,Mohamed H. Helal,Zhuyun Yan,Zhanhu Guo,Zhengjun Shi
Acid polysaccharide was extracted from Salvia przewalskii root powders (PSP), purified by diethylaminoethyl cellulose column (DEAE-52) and molecular sieve (PSP2). PSPm1 was obtained by modifying PSP2 with nitrite and phosphoric acid. The chemical structure of PSP2 and PSPm1 exhibited notable distinctions, primarily due to the absence of arabinose and promotion of glucuronic acid (GlcA). The structure of PSPm1 was deduced through the utilization of 1 H, 13 C, and 2-D NMR. The main chain was linked by α-D-Gal p (1 → 3)-α-Glc p -(1 → fragments and →6)-β-D-Gal p fragments, with the presence of →4)-α-D-Glc p A-(1 → 6)-β-D-Gal p -(1 → ， → 4)-α-D-GalA p -(1 → 2,4)-α-D-Rha p -(1 → fragments and →6)-α-Glc p -(1 → 2,4)-β-D-Man p -(1 → fragments. PSPm1 exhibited different immunoregulatory bioactivity in vitro, including haemostatic effects indicated by activated clotting time of 55.5 % reduction by the activated clotting time (ACT) test and wound healing function in vivo. PSPm1 also displayed better anti-tumor biological effects than unmodified. The structure-activity dissimilarity between PSP2 and PSPm1 primarily stems from variations in molecular weight ( Mw ), monosaccharide composition, and branching patterns. The modification of polysaccharides from the extract residues of Chinese medicinal materials may be a new form of drug supplements.
研究领域:
药理学
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生物化学
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分子生物学
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药物化学
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PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2019
·
IF 4.784
Jing Qin,Hui-yang Wang,Dan Zhuang,Fan-cheng Meng,Xing Zhang,He Huang,Guang-ping Lv
A neutral polysaccharide (ALP-1) and an acidic polysaccharide (ALP-3) were purified from Atractylodes lancea (Thunb.) DC. ALP-1 exhibited a linear backbone composed of (2 → 1)-linked β - d -fructofuranose. The backbone of ALP-3 was elucidated as →4)-GalA p -(1 → 3,4)-Rha p -(1→, and with branch chain substituted at O-3 position of →3,4)-Rha p -(1→. The branch chain consists of →3,5)-Ara f -(1→, →5)-Ara f -(1→, and Ara f -(1→. Particularly, ALP-3 could significantly stimulate macrophage proliferation in a dose-dependent manner, and stimulate phagocytic, NO and cytokines production than ALP-1 on RAW264.7 cells. Besides, both ALP-1 and ALP-3 could activate T-cells in Peyer's patch cells and enhance the production of colony stimulation factors. While the ALP-3 also showed better intestinal immune system modulating activity than ALP-1. The result of this study indicated that the structure diversity of polysaccharide is crucial for its bioactivity. And also provided evidence for that carbohydrate polymers in A. lancea definitely contributed to its pharmaceutical effects.
研究领域:
药理学
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免疫学
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植物化学
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DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
·
2024
·
IF 11
Xiaofei Liang,Mengqiu Liu,Anni Yao,Wanchen Cui,Yan Wei,Sheng Guo,Jialun Duan,Hongjie Kang,Xueyi Zhou,Shulan Su,Heiying Jin,Fang Zhang,Jin-ao Duan
In this study, the microbial fermentation characteristics and effects of neutral and acidic Lycii fructus polysaccharides (LFPs) on gut microbial composition were investigated using pure cultures of selected probiotic strains and mixed cultures of human fecal microflora in vitro . The results revealed that LFPs could significantly promote the growth of probiotics, with a faster proliferation rate in the early stages of fermentation. When LFPs were degraded and utilized by the gut microbiota, neutral arabinogalactans were more easily fermented, while acidic pectin polysaccharides exhibited a higher degree of anti-fermentation. LFPs were selectively modulated by microbiota; acidic polysaccharides promoted the growth of potentially beneficial bacteria, such as Lachnospira and Bacteroides vulgatus , while neutral polysaccharides significantly improved the relative abundances of Bifidobacterium longum and Bifidobacterium pseudocatenulatum . In summary, the results revealed that different types of LFPs regulate the gut microbiota in a structure-dependent manner, with uronic acid play a key role in selective regulation. This study aimed to better understand the fermentation characteristics and saccharide preferences of gut microbes for LFP structures and provide a theoretical basis for the relationship between the chemical structural and probiotic effects of LFPs.
研究领域:
微生物学
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营养学
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食品科学
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胃肠病学
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DOI
高影响因子
PLANT FOODS FOR HUMAN NUTRITION
·
2023
·Vol. 78
·期 2
·390-398
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IF 4
Jing Yongshuai,Su Ziteng,Zhang Shilin,Han Qiyuan,Wang Ziying,Hu Beibei,Zhang Danshen,Sun Shiguo,Wu Lanfang
An acidic polysaccharide (SMP) with a molecular weight (Mw) of 1.28 × 10 6 Da was isolated from Salvia miltiorrhiza. The monosaccharide composition in molar percentages was rhamnose (Rha): galacturonic acid (GalA): galactose (Gal): arabinose (Ara) = 6.15: 55.98: 21.27: 16.69. The results of simulated digestion in vitro showed that SMP was not degraded in saliva, gastric juice or intestinal juice. The Y maze test and new object recognition test showed that SMP could improve the working memory impairment of aging mice. SMP could also increase the activity of superoxide dismutase (SOD) and catalase (CAT) in serum and brain tissue, decrease the content of malondialdehyde (MDA), decrease the levels of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in brain tissue, and increase the content of short-chain fatty acids (SCFA) in the intestine. In addition, SMP could also regulate the intestinal flora structure, including increasing the relative abundance of Firmicutes and Bacteroidetes and decreasing the relative abundance of Proteobacteria . This work lays a foundation for the development of functional foods related to Salvia miltiorrhiza .
研究领域:
药理学
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营养学
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老年学
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免疫学
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微生物学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2021
·
IF 6.953
Fengwei Ma,Hongbing Liu,Su Xu,Yongyou Cheng,Qiang Fei,Haijiang Chen
A water-soluble polysaccharide (LCP-05) was isolated from the flowers of Leucosceptrum canum Smith. LCP-05 was an acidic polysaccharide with a molecular weight of approximately 8.9 kDa. Monosaccharide composition analysis indicated that LCP-05 was composed of Man, Rha, GlcA, GalA, Glc, Gal and Ara in a molar ratio of 0.83:1.68:0.33:2.15:1.00:1.45:1.22. The framework of LCP-05 was speculated to be a branched rhamnogalacturonan with the backbone consisting of α-1,2,4-linked Rha p and α-1,4-linked GalA p , and bearing branches at the O -4 position of the Rha residues. The side chains are terminated primarily with the Ara f and Glc p residues. LCP-05 was found to be able to significantly induce the production of NO, IL-6, and TNF-α in RAW 264.7 cells, and to induce RAW 264.7 cell's suppressive effect on both cell growth and cell migration of 4 T1 mammary breast cancer cells.
研究领域:
植物学
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免疫学
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药理学
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癌症研究
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DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）