INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Xiao-Jun Li,Shi-Jun Xiao,Yi Heng Xie,Jiang Chen,Hai-Rong Xu,Yuan Yin,Rui Zhang,Tong Yang,Tong-Yu Zhou,Si-Yan Zhang,Pei Hu,Li-Ming Gao,Hui-Ping Peng
An innovative acidic hydrolysate fingerprinting workflow was proposed for the characterization of Lyophyllum Decastes polysaccharide (LDP) by ultra performance liquid chromatography-mass spectrometry (UPLC-MS). The crude polysaccharides were firstly separated and purified by using DE-52 column and the BRT GPC purification system, respectively. The molecular weight and monosaccharide content of homogeneous polysaccharides were ascertained by utilizing HPGPC and ion chromatography separately. Secondly, the linkage of LDP was identified by methylation analysis and 1D/2D NMR spectra. The UPLC-MS/MS was used to scan and identify the acidic hydrolysate products of LDP using the PGC column. The oligosaccharides were collected by chromatography and identified by mass spectrometry. Thirdly, the expression of IL-1β, IL-6, iNOS, TNF-α and IFNAR-I was measured in order to assess the immunological activity of LDP. Besides, the targeted receptors identification of polysaccharides was performed by screening the expression of TLRs family protein. The results showed that oligosaccharide fragments with different molecular weights can be obtained by partial hydrolysis, which further verified that the structures of LDP polysaccharides was a 1–6-linked β-glucan. Moreover, the LDP polysaccharide can up-regulate the content of IL-1β, IL-6, iNOS, TNF-α and IFNAR-I and plays an important immunoregulation role through TLRs family.
研究领域:
生物化学
·
免疫学
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DOI
PubMed
高影响因子
Inorganics
·
2025
·Vol. 13
·期 3
·89
·
IF 3
Bosen Zhou,Zhengbo Lai,Yuanyuan Li,Hualan Zhou,Ye Tian,Yibo Zhao,Ming Xia
The siliceous precursor was hydrolyzed from tetraethylorthosilicate (TEOS) under acidic conditions, followed by the addition of sodium aluminate and sodium hydroxide. Y zeolite was subsequently obtained through hydrothermal crystallization under alkaline conditions. Key synthesis parameters, including reactant molar ratios, crystallization temperature, and time, were systematically varied to optimize the synthesis conditions. The synthesized products were characterized using X-ray diffraction (XRD), scanning electron microscopy (SEM), nitrogen adsorption analysis, and inductively coupled plasma (ICP) elemental analysis. Well-crystallized Y zeolite with a silica-alumina ratio (SAR) of 5.55 was successfully synthesized via TEOS hydrolysis catalyzed by sulfuric acid at a low crystallization temperature of 85 °C. The catalytic performance of benzyl phenyl ether, a lignin model compound, over NiY catalyst was evaluated in a high-pressure reactor. The results indicate that the catalytic efficiency of Y zeolite synthesized using TEOS as the silicon source under acidic hydrolysis conditions is significantly superior to Y zeolite prepared using alkaline silica sol as the silicon source.
研究领域:
化学工程
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催化材料科学
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DOI
高影响因子
CARBOHYDRATE POLYMERS
·
2014
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IF 3.916
Jiachang Huang,Li Zhou
The hydrolyzed peach gum polysaccharide (HPGP) prepared at acidic condition is investigated as an anionic polyelectrolyte for the first time. A hydrolysis mechanism is proposed by monitoring the hydrolysis efficiency and morphological change of crude peach gum, and the intrinsic viscosity of resulted HPGP as a function of hydrolysis time. Fourier transform infrared (FTIR) spectroscopy and ζ-potential measurements reveal that HPGP with multiple carboxylic groups is negatively charged in water in the pH range 3–11. The HPGP exhibits remarkable pH and ionic strength responsiveness, as proven by dynamic light scattering (DLS) and atomic force microscopy (AFM) measurements. Moreover, the layer-by-layer (LbL) self-assembly experiments further confirmed that the HPGP can be utilized as an anionic polyelectrolyte . Considering the facile availability, favorable compatibility and intriguing functionality of HPGP, this study opens up enormous opportunities for the large-scale utilization of peach gum resource.
研究领域:
高分子科学
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生物材料
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纳米技术
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药物递送
·
表面化学
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DOI
高影响因子
Food Science & Nutrition
·
2021
·Vol. 9
·期 6
·3059-3070
·
IF 2.863
Hongliang Yao,Jiani Yang,Jiajia Zhan,Qu Lu,Min Su,Yaojiong Jiang
To improve the utilization of okra seed, acidic and enzymatic hydrolyses of producing protein hydrolysates were respectively optimized by orthogonal experiment and response surface methodology using the degree of hydrolysis (DH) as evaluating index. Amino acid composition and antioxidant capacity in vitro of two kinds of hydrolysates were both analyzed. The degree of acidic hydrolysis was 58.53 ± 1.92% under the following optimized condition: hydrolyzing time 40 hr, temperature 95°C, ratio of acid solution to okra seed meal (OSM) powder was 5:1 (V:W/ml:g), and hydrochloric acid concentration was 18% (W/W). The degree of enzymatic hydrolysis was 16.26 ± 0.56% under the optimized condition: hydrolyzing time 8.20 hr, ratio of buffer to OSM powder was 10:1, and enzyme dosage was 3,100 International Units (IU) g −1 . Enzymatic hydrolysates had a fuller range of amino acids and antioxidant capacity than acidic hydrolysates. The results provide technical support for the expansion of okra seed utilization.
研究领域:
食品科学
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营养学
·
生物化学
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DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2020
·
IF 4.006
Xingjie Zhang,Kexue Zhu,Jianhua Xie,Yi Chen,Lehe Tan,Shuai Liu,Ruihong Dong,Yuting Zheng,Qiang Yu
The study presented the first comparison of content and composition along with the antioxidant activities of non-extractable polyphenols (NEPP) in the dietary fiber of jackfruit pulp (JDF) released by alkaline, acid and enzymatic hydrolysis . Firstly, the NEPP extraction was optimized by response surface methodology (RSM), and the alkaline hydrolysis extract contained the most abundant content. Besides, the composition of extracts were characterized by UPLC-ESI-QTOF-MS/MS and the alkaline hydrolysis extract possessed most diverse NEPP (4 phenolic acids and 13 flavonoids). Moreover, the antioxidant activities of NEPP were tested by ABTS ·+ , ORAC and PSC scavenging ability assays, the antioxidant activities of alkaline hydrolysis extract were significantly highest among three extracts. This study provided available evidence for the NEPP released from dietary fiber (DF) by different methods, and revealed alkaline hydrolysis extract promising potential as a natural source of health care products.
研究领域:
食品科学
·
植物化学
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Yue Qiu,Jiayan Xie,Jing Liang,Jianhua Xie,Yi Chen,Xiaobo Hu,Nan Sun,Xiling Wang,Qiang Yu
In this paper, the optimal conditions for extracting dietary fiber-bound polyphenols in Canavalia gladiata seed coat using three hydrolysis methods, namely alkali, acid and enzyme, were obtained using response surface methodology. The total phenolic content of alkali hydrolysis products was 2635.97 μg GAE/g DW, which was much higher than that of the acid (1418.87 μg GAE/g DW) and the enzyme method (1316.39 μg GAE/g DW). Qualitative analysis identified a total of 38 compounds, 33 of which were quantified. The results showed that CGDF-BPs were dominated by phenolic acids, especially gallic acid. Overall the alkaline method hydrolyzed dietary fibers more thoroughly, releasing a higher diversity of compounds. However, their in vitro antioxidant and α-glucosidase inhibitory activities were inconsistent at equivalent concentration due to differences in bound polyphenol composition caused by the alkali, acid, and enzyme methods, which served as a reference for studying of the composition and activity of natural plant polyphenols.
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）