Food and Bioprocess Technology
·
2019
·Vol. 12
·期 11
·1907-1916
·
IF 3.032
Zhou Lei,Liao Tao,Liu Junping,Zou Liqiang,Liu Chengmei,Liu Wei
The activity of polyphenoloxidase (PPO) treated by acidic pH and mild thermal processing was found to be closely related to the conformational changes. Weakly acidic environment (pH 4.0–6.0) resulted in reversible inhibition of activity and slight changes in the conformation, and refolding of PPO was observed after readjusting pH to 6.8. At pH lower than 4.0, PPO activity was strongly inhibited with great unfolding in conformation and all changes were irreversible. Acidic environment increased the susceptibility of PPO to thermal treatment, and greater changes in activity and conformation of PPO were observed under the combined treatment of acidic pH and mild thermal treatment. Besides, the inactivation of PPO induced by thermal treatment followed a biphasic kinetic model, and acidic pH increased the inactivation rate of labile and stable PPO. The combination of acidic pH and mild thermal processing expected to avoid the nutritional loss and sensory damage in fruits and vegetables caused by severe acid or temperature.
研究领域:
食品科学
·
生物化学
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产品关联
DOI
高影响因子
Bioactive Materials
·
2022
·
IF 16.874
Yue Xu,Yuan You,Luyao Yi,Xiaoyi Wu,Yaning Zhao,Jian Yu,He Liu,Ya Shen,Jingmei Guo,Cui Huang
Dental caries is one of the most prevalent human diseases resulting from tooth demineralization caused by acid production of bacteria plaque. It remains challenges for current practice to specifically identify, intervene and interrupt the development of caries while restoring defects. In this study, inspired by natural dental plaque, a stimuli-responsive multidrug delivery system ( [email protected] ) has been developed to prevent tooth decay and promote enamel restoration. Classic spherical core-shell structures of micelles dual-loaded with antibacterial and restorative agents are self-assembled into bacteria-responsive multidrug delivery system based on the pH-cleavable boronate ester bond, followed by conjugation with salivary-acquired peptide (SAP) to endow the nanoparticle with strong adhesion to tooth enamel. The constructed [email protected] specifically adheres to tooth, identifies cariogenic conditions and intelligently releases drugs at acidic pH, thereby providing antibacterial adhesion and cariogenic biofilm resistance, and restoring the microarchitecture and mechanical properties of demineralized teeth. Topical treatment with [email protected] effectively diminishes the onset and severity of caries without impacting oral microbiota diversity or surrounding mucosal tissues. These findings demonstrate this novel nanotherapy has potential as a promising biomedical application for caries prevention and tooth defect restoration while resisting biofilm-associated diseases in a controlled manner activated by pathological bacteria.
研究领域:
口腔医学
·
纳米医学
·
生物材料学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Shengtao Zhang,Bin Li,Jieyu Zhou,Jiayi Shi,Zhongjing He,Yumin Zhao,Yan Li,Yehua Shen,Yongmin Zhang,Shaoping Wu
In this work, tea saponin (TS) which is indispensable in Camellia oleifera industry was used to synthesize a class of hydrophobic carbon dots (TS-CDs) with aggregation-induced emission (AIE) properties. A new fluorescent sensing platform based on AIE and non-modified TS-CDs for the detection of human serum albumin (HSA) and pH was developed, respectively. Interestingly, the developed platform is capable of ratiometric detecting extremely acidic pH in the range of 0.2–1.8 linearly (R 2 = 0.9959) due to protonation-deprotonation. Meanwhile, TS-CDs exhibited well stability toward HSA detection over a wide linear range (0–180 μM), long-term (48 h), and wide pH range (2–13). Furthermore, TS-CDs was utilized to localize to mitochondria and detect HSA in living cells, demonstrating its promising perspective in biosensing applications. This work may pave a novel avenue for high value-added utilization in the extraction process of extracting camellia oil for food woody oil.
研究领域:
生物化学
·
材料科学
·
医学检测
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产品关联
DOI
高影响因子
DYES AND PIGMENTS
·
2021
·
IF 4.889
Yingying Zhang,Yanliang Zhao,Bo Song,Chaobo Huang
Acidic pH is an important physiological parameter in environment and biological systems. Thus, designing a probe ( Hcy-F ) with good characters to monitor acidic pH is really important. Herein, a small molecular water-soluble probe is designed based on indole with high selectivity and sensitivity for detection the acidic pH in aqueous solution and living cells. When pH variation of aqueous is detected by probe, an obvious ratiometric and good linear response behavior are found in UV–vis absorption spectrum (R 2 = 0.9973, A 434 nm / A 371 nm = 6.9929–4.2231 pH) and fluorescence emission spectrum (R 2 = 0.9987, I 571 nm / I 514 nm = 7.2935–2.981 pH), which further proved the feasibility of Hcy-F to monitor the change of acidic pH in aqueous, respectively. Furthermore, Hcy-F is successfully applied for monitoring pH variation in living cells in dual channels (green and red) with higher cell survival rate, indicating the probe has great potential for pH imaging in biological applications .
研究领域:
分析化学
·
生物化学
·
细胞生物学
✓ 已验证
产品关联
DOI
高影响因子
Journal of Translational Medicine
·
2024
·
IF 6.1
Wenbin Hua,Lin Xie,Chenpeng Dong,Guoyu Yang,Shouyuan Chi,Zhiqiang Xu,Cao Yang,Huiwen Wang,Xinghuo Wu
Intervertebral disc degeneration (IVDD) is a common cause of low back pain. Procyanidin C1 (PCC1) has been demonstrated to exert a protective effect on nucleus pulposus (NP) cells, and therefore, plays a critical role in the prevention and therapy of IVDD. Clarifying the pathophysiological characteristics and molecular mechanisms of IVDD may be helpful in establishing novel preventive and therapeutic strategies. This study aimed to investigate the probable mechanisms underlying the protection against acidic pH stress induced human NP cell injury. In vitro, acidic pH stress induced degeneration, mitochondrial dynamics imbalance, mitophagy, and mitochondria-mediated apoptosis in NP cells, all of which were ameliorated by PCC1. Autophagy inhibition partially eliminated the protective effects of PCC1 on mitochondrial homeostasis in NP cells. Moreover, PCC1 activated the sirtuin 3 (SIRT3)/forkhead box O3 (FOXO3) signaling pathway, a pivotal signaling pathway involved in the regulation of mitochondrial homeostasis in NP cells. In vivo, PCC1 ameliorated IVDD in a rat model and preserved the extracellular matrix of NP cells. Consequently, the protective effects of PCC1 on NP cells may inhibit IVDD progression via regulation of the SIRT3/FOXO3 signaling pathway. Therefore, regulation of the SIRT3/FOXO3 signaling pathway may be a novel preventive and therapeutic strategy for IVDD.
研究领域:
骨科学
·
分子生物学
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细胞生物学
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DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2014
·
IF 4.258
Daojin Li,Qianjin Li,Shuangshou Wang,Jin Ye,Hongyuan Nie,Zhen Liu
Boronate affinity chromatography (BAC) is a unique means for the selective separation and enrichment of 1,2 and 1,3 cis -diol-containing compounds. However, conventional boronate affinity materials require a basic binding pH (usually ≥ 8.5), which gives rise to not only inconvenience in operation but also the risk of degradation of labile compounds. Although the applicable pH has been expanded to 5.0 in recent years, the current boronate affinity materials still fail to meet the acidic pH end of frequently used biosamples, particularly urine (pH 4.5). In this study, we report a 3-pyridylboronic acid-functionalized organic–silica hybrid monolithic capillary that exhibited a binding pH of 4.5, the lowest so far in BAC. Such a binding pH enabled direct extraction of cis -diol-containing biomolecules such as nucleosides from urine samples without pH adjustment. The boronate affinity monolithic capillary showed enhanced affinity toward negatively charged cis -diol-containing analytes such as ribonucleotides. Moreover, it could function as an anion exchanger at acidic pH (∼2). The column was found to retain multiple compounds from urine, which can be assumed to be at least mostly if not entirely cis -diol-containing compounds.
研究领域:
分析化学
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生物化学
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产品关联
DOI
PubMed
高影响因子
Journal of Integrative Agriculture
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2022
·
IF 4.384
Muhammad Irfan WARIS,Aneela YOUNAS,Rana Muhammad Kaleem ULLAH,Fatima RASOOL,Muhammad Muzammal ADEEL,Man-qun WANG
Chemosensory proteins (CSPs) are important molecular components of the insect olfactory system, which are involved in capturing, binding, and transporting hydrophobic odour molecules across the sensillum in sensillar lymph in regulating insect behavior. This protein family (CSPs) is also involved in many other systems that are not linked to olfactory receptors in olfactory sensilla. The brown planthopper (BPH) is a monophagous pest of rice that causes damage by sucking phloem sap and transmitting a number of diseases caused by viruses. In this study, fluorescence competitive binding assay and fluorescence quenching assay at acidic pH were performed as well as homology modelling to describe the binding affinity of NlugCSP10. Fluorescence competitive binding assay (FCBA) demonstrated that NlugCSP10 bound strongly to nonadecane, farnesene, and 2-tridecanone at acidic pH. The results of FCBA indicated that NlugCSP10 bound different ligands at the physiological pH (5.0) of the bulk sensillum lymph. Fluorescence quenching assay demonstrated that NlugCSP10 generated a stable complex with 2-tridecanone, while two ligands nonadecane and farnesene collided due to molecular collisions. The interaction of selected ligands with the modelled structure of NlugCSP10 was also analyzed, which found the key amino acids (Gln23, Gln24, Gln25, Asn27, Met33, Ser34, Ile35, Tyr36, Asn42, Met43, Val45, Asn46, Asn93, Arg96, Ala97, Lys99, and Ala100) in NlugCSP10 that were involved in binding of volatile compounds. The present study contributes to the binding profile of NlugCSP10 that promotes the development of behaviorally active ligands based on BPH olfactory system.
研究领域:
昆虫学
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分子生物学
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生物化学
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DOI
高影响因子
WATER ENVIRONMENT RESEARCH
·
2019
·Vol. 91
·期 7
·634-641
·
IF 1.24
Xiao Zhang,Jing Zhang,Xue Huang,Qing Ping Wu,Chun Hui Yan,Jin Feng Lu
ZnO/Fe 3 O 4 @carbon spheres, which synthesized via a calcination process used Zn/Fe metal–organic frameworks (Zn/Fe–MOFs) as a precursor, were studied for the activation of peroxymonosulfate (PMS) for the degradation of Acid Orange 7 (AO7). The ZnO/Fe 3 O 4 @carbon spheres exhibited relatively high catalytic degradation properties for AO7 in an aqueous solution. The AO7 degradation reached 93.6% in 15 min under the conditions: 0.20 g/L ZnO/Fe 3 O 4 @carbon spheres, 1.25 mmol/L PMS, 0.03 mmol/L AO7, and initial pH of 4. Findings revealed that higher ZnO/Fe 3 O 4 @carbon spheres dose and PMS concentration, lower initial AO7 concentration, and acidic pH favored the AO7 degradation to a certain extent. The mechanisms for the activation of PMS by ZnO/Fe 3 O 4 @carbon spheres were proposed based on the results of radical identification tests and structure characterization. Both radical and nonradical pathways contribute to the AO7 degradation in this system. Practitioner points PMS + ZnO/Fe 3 O 4 @carbon spheres system can effectively catalyze PMS to decompose AO7. Both radical and nonradical pathways contribute to the degradation of AO7 in this system. The acidic condition was favorable for the activation of PMS.
研究领域:
环境科学
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化学
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材料科学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
·
2022
·
IF 3.277
Chengwei Yang,Ting Zhang,Jing Zhu,Yunyi Wei,Furong Zhu,Zhong Cheng
Exo-polygalacturonase (exo-PG) hydrolyzes pectin acids and liberates mono-galacturonate, which plays an important role in juice extraction, and has rarely been reported. Exo-PG (AfumExoPG28A) from Aspergillus fumigatus belongs to the glycoside hydrolase 28 family. In this study, its gene was cloned and the protein was expressed and secreted in Pichia pastoris with a maximal activity of 4.44 U/ml. The optimal temperature and pH of AfumExoPG28A were 55°C and 4.0, respectively. The enzyme exhibited activity over almost the entire acidic pH range (>20.0% activity at pH 2.5-6.5) and remained stable at pH 2.5–10.0 for 24 h. The K m and V max values of AfumExoPG28A were calculated by the substrate of polygalacturonic acid as 25.4 mg/ml and 23.6 U/mg, respectively. Addition of AfumExoPG28A (0.8 U/mg) increased the light transmittance and juice yield of plantain pulp by 11.7% and 9%, respectively. Combining AfumExoPG28A (0.8 U/mg) with an endo-PG (0.8 U/mg) from our laboratory, the enzymes increased the light transmittance and juice yield of plantain pulp by 45.7% and 10%, respectively. Thus, the enzyme’s potential value in juice production was revealed by the remarkable acidic properties and catalytic activity in fruit pulp.
研究领域:
酶学
·
食品科学
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微生物学
·
生物技术
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产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2021
·Vol. 9
·期 38
·8109-8120
·
IF 6.331
Hao Liu,Haolin Chen,Xiaoying Liu,Luoqi Mo,Chao Chen,Zhouyi Guo,Zhiming Liu
1T-phase niobium telluride (NbTe2) nanosheets are becoming increasingly important in emerging fields, such as spintronics, sensors and magneto-optoelectronics, due to their excellent physical and chemical properties. However, exploration on their biomedical applications are limited. Herein, ultrathin 1T-phase NbTe2 single-crystalline nanosheets with excellent photothermal performance, high drug-loading rate, near-infrared (NIR) light/acidic pH-triggered drug release, and low toxicity were developed for potentiated photothermal therapy. Importantly, they showed excellent biocompatibility in vivo and in vitro. NbTe2 nanosheets loaded with integrated stress response inhibitors (ISRIB) could achieve chemo-photothermal therapy of tumors through the ATF4–ASNS signaling axis. Ultrathin 1T-phase NbTe2 single-crystalline nanosheets with unique photothermal properties, drug loading rate and safety provide dramatic possibilities in biomedical applications, such as tissue imaging, photothermal therapeutics and pharmaceutics.
研究领域:
生物医学工程
·
纳米技术
·
癌症治疗
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2025
·
IF 9.7
Longhao Lv,Yong Liu,Chengsong Cao,Yong Li,Zhengshuai Tang,Jinliang Liu
The tumor microenvironment (TME) is characterized by several key features, including hypoxia, elevated levels of hydrogen peroxide (H 2 O 2 ), high concentrations of glutathione (GSH), and an acidic pH. Recent research has increasingly focused on harnessing or targeting these characteristics for effective cancer therapy. In this study, we developed an innovative composite bio-reactor that integrates genetically engineered bacteria with upconversion nanoparticles (UCNPs) and nano-copper manganese materials for lung cancer treatment. The nano-copper manganese materials function as catalysts in Fenton-like reactions, facilitating the decomposition of hydrogen peroxide into harmful hydroxyl radicals and oxygen, which can effectively target tumors and reduce hypoxia. To circumvent the challenge of insufficient endogenous hydrogen peroxide during treatment, we employed UCNPs capable of converting near-infrared laser irradiation, known for its deep tissue penetration, into visible light. This conversion activates the genetically engineered bacteria to generate exogenous hydrogen peroxide directly within the tumor microenvironment, enabling prolonged therapeutic effects. Our findings suggest that this composite bio-reactor can achieve effective lung cancer therapy without the need for external hydrogen peroxide supplementation, representing a significant advancement in the design of targeted cancer treatments.
研究领域:
肿瘤学
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纳米医学
·
生物工程
·
癌症治疗
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Shuangjun Li,Ruyue Shi,Yankun Liu,Mingcong Niu,Zhicun Liu,Zhongjuan Wang,Jiwang Gao,Hongda Cai,Zhuyu Zhou,Shengnan Li,Xiuhua Zhao
Immune cells infiltrating tumors play a pivotal role in tumor progression regulation; however, their functionality is frequently suppressed by the acidic conditions of the tumor microenvironment (TME). Targeting and reversing tumor acidity holds significant potential for enhancing the efficacy of immunotherapy. Here, we present a proof-of-concept study on tumor acidity-reversal therapy mediated by bacterial cellulose-targeted micelles to promote M2 macrophage repolarization to M1 macrophages and restore the anti-tumor function of T cells. Our strategy utilizes a bacterial cellulose (BC) composite modified with folic acid (FA) and chrysin (BY), loaded with lactic acid-neutralizing chemotherapeutic vincristine (VCR) and lactate dehydrogenase inhibitor GNE-140, to self-assemble into tumor-targeted nanoparticles. Post-treatment, FA-BC-BY@VCR@GNE-140 demonstrated notable efficacy in reversing tumor acidity, inducing tumor cell death, mitigating immunosuppression, and stimulating host adaptive immunity. This approach represents an innovative strategy for synergizing chemotherapy and immunotherapy to combat tumor progression.
研究领域:
肿瘤免疫学
·
纳米医学
·
药物递送
·
肿瘤微环境
·
免疫治疗
·
生物材料
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）