JOURNAL OF FOOD SCIENCE
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2017
·Vol. 82
·期 8
·1978-1984
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IF 1.815
Huan bin Zhou,Jiashu Chen,Shunyi Li,Jianpan Zhang,Chun e Zhu,Hao Ran,Meihua Luo,Xin Pan,Haiyan Hu,Chuanbin Wu
en This link goes to a English section pt This link goes to a English section Microencapsulation is an effective technology used to protect probiotics against harsh conditions. Extrusion is a commonly used microencapsulation method utilized to prepare probiotics microcapsules that is regarded as economical and simple to operate. This research aims to prepare acid-resistant probiotic microcapsules with high viability after freeze-drying and optimized storage stability. Streptococcus thermophilus IFFI 6038 (IFFI 6038) cells were mixed with trehalose and alginate to fabricate microcapsules using extrusion. These capsules were subsequently coated with chitosan to obtain chitosan-trehalose-alginate microcapsules with shell-matrix structure. Chitosan-alginate microcapsules (without trehalose) were also prepared using the same method. The characteristics of the microcapsules were observed by measuring the freeze-dried viability, acid resistance, and long-term storage stability of the cells. The viable count of IFFI 6038 in the chitosan-trehalose-alginate microcapsules was 8.34 ± 0.30 log CFU g −1 after freeze-drying (lyophilization), which was nearly 1 log units g −1 greater than the chitosan-alginate microcapsules. The viability of IFFI 6038 in the chitosan-trehalose-alginate microcapsules was 6.45 ± 0.09 log CFU g −1 after 120 min of treatment in simulated gastric juices, while the chitosan-alginate microcapsules only measured 4.82 ± 0.22 log CFU g −1 . The results of the long-term storage stability assay indicated that the viability of IFFI 6038 in chitosan-trehalose-alginate microcapsules was higher than in chitosan-alginate microcapsules after storage at 25 °C. Trehalose played an important role in the stability of IFFI 6038 during storage. The novel shell-matrix chitosan-trehalose-alginate microcapsules showed optimal stability and acid resistance, demonstrating their potential as a delivery vehicle to transport probiotics. Practical Application en This link goes to a English section pt This link goes to a English section The construction of acid-resistant shell-matrix microcapsules is a novel assay in the development and application of microencapsulation technology in probiotics. The method of embedded cryoprotectants in microcapsules enhances the thermal stability and freeze-dried viability of enzymes in Streptococcus thermophiles compared to conventional methods that simply mix the cryoprotectants and microcapsules together.
研究领域:
食品科学
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微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Clinical Oral Investigations
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2018
·Vol. 23
·期 5
·2129-2141
·
IF 2.386
Jung Jae-Hyun,Park Soo-Byung,Yoo Kyung-Hyeon,Yoon Seog-Young,Bae Moon-Kyoung,Lee Dong Joon,Ko Ching-Chang,Kwon Yong Hoon,Kim Yong-Il
Objectives To synthesize two different sizes of bioactive glass-coated mesoporous silica nanoparticles (BGN@MSNs) and to investigate their effects on dentinal tubule occlusion and remineralization. Materials and methods Two different sizes of mesoporous silica nanoparticles (MSNs) were synthesized using the Stöber method (368A, 1840A) and coated with bioactive glass nanoparticles (BGNs) using a modified quick alkali-mediated sol-gel method (368B, 1840B). Sensitive tooth disc models were prepared and divided into six groups and the following treatments were applied: group 1—no treatment, group 2—bioglass, group 3—368A, group 4—368B, group 5—1840A, and group 6—1840B. Then, five discs were selected from each group and soaked in 6 wt% citric acid to test acid resistance. Dentinal tubule occlusion and occlusion ratio were observed using field-emission scanning electron microscopy. In vitro mineralization tests using simulated body fluid solution were performed to evaluate the remineralization effect of the treatment. Results All samples effectively occluded the dentinal tubule and formed a membrane-like layer. After acid treatment, 1840B (group 6) exhibited the highest rate of dentinal tubule occlusion. Remineralization was observed in 368B and 1840B, and 1840B exhibited the fastest remineralization. Conclusions Dentinal tubule remineralization induced by the BGN@MSN biocomposite can be used to stabilize long-term prognosis in dentin hypersensitivity. The 1840B induced the most efficient remineralization, and its smaller size and larger surface area were effective for remineralization. Clinical relevance The BGN@MSN biocomposite with its smaller size and larger surface area was more effective for remineralization and dentinal tubule sealing.
研究领域:
牙科材料学
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纳米医学
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再生医学
✓ 已验证
DOI
PubMed
高影响因子
Microbiology Spectrum
·
2022
·Vol. 11
·期 1
·
IF 9.043
Xiayu Liu,Xinxin Pang,Yansha Wu,Yajing Wu,Linan Xu,Qihe Chen,Jianrui Niu,Xinglin Zhang
Listeria monocytogenes is a foodborne pathogen that can tolerate a variety of extreme environments. In particular, its acid resistance (AR) capability is considered one of the key factors threating food safety. Here, we employed a microbial functional genomic technology termed transposon sequencing (Tn-seq), leading to the identification of two genes involved in cell wall peptidoglycan biosynthesis (murF) and phosphate transport (lmo2248) that play key roles in lactic acid resistance (LAR) of L. monocytogenes. Deletion of lmo2248 significantly impaired the ability of LAR in L. monocytogenes, demonstrating the accuracy of the Tn-seq results. Transcriptome analysis revealed that 31.7% of the L. monocytogenes genes on the genome were differentially expressed under lactic acid (LA) treatment, in which genes involved in phosphate transport were influenced most significantly. These findings shed light on the LAR mechanisms of L. monocytogenes, which may contribute to the development of novel strategies against foodborne pathogens.IMPORTANCE Listeria monocytogenes is a Gram-positive foodborne pathogen with high lethality and strong stress resistance, and its strong acid tolerance leads to many foodborne illnesses occurring in low-pH foods. Lactic acid is a generally recognized as safe (GRAS) food additive approved for use by the FDA. However, the genetic determinants of lactic acid resistance in L. monocytogenes have not been fully identified. In this study, the lactic acid resistance determinants of L. monocytogenes were comprehensively identified by Tn-seq on a genome-wide scale. Two genes, murF (cell wall peptidoglycan biosynthesis) and lmo2248 (phosphate transport), were identified to play an important role in the lactic acid resistance. Moreover, genome-wide transcriptomic analysis showed that phosphotransferase system (PTS)-related genes play a key role at the transcriptional level. These findings contribute to a better understanding of the lactic acid resistance mechanism of L. monocytogenes and may provide unique targets for the development of other novel antimicrobial agents.
研究领域:
微生物学
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功能基因组学
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食品安全
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
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2021
·
IF 7.312
Zhenjie Gu,Zibo Yang,Xiangyu Guo,Zhihua Qiao,Chongli Zhong
CH 4 /N 2 separation, a viable way to purify energy gas and reduce the greenhouse effect, requires the use of highly gas permeable membrane, but acid environment affects the membrane stability. In this work, the ZIF-8@VR nanoparticle was synthesized via a high vacuum resistance sintering method (VRSM), which increased the acid resistance to pH 2.8 because of surface carbonization and stiffening. The structure of the ZIF-8@VR nanoparticle was investigated by XRD, SEM, FTIR, BET and Raman analysis, followed by blending it with polyacrylic acid (PAA) to fabricate PAA/ZIF-8@VR mixed matrix membranes (MMMs). The MMMs exhibited excellent CH 4 permeance and satisfactory CH 4 /N 2 selectivity, with the optimal CH 4 permeance (~1763 GPU) for the MMM with 66.8 wt% ZIF-8@VR filler. Furthermore, compared with pure PAA membrane, the MMMs with ZIF-8@VR filler could improve the CH 4 /N 2 selectivity from 1.3 to 3.1.
研究领域:
材料科学
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化学工程
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环境科学
✓ 已验证
DOI
高影响因子
Journal of Materials Chemistry B
·
2023
·Vol. 11
·期 23
·5207-5222
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IF 7
Panxianzhi Ni,Sheng Ye,Shuting Xiong,Meng Zhong,Jing Shan,Tun Yuan,Jie Liang,Yujiang Fan,Xingdong Zhang
Ulcerative arterial bleeding is characterized by sudden onset, rapid disease development, and high mortality, which is a great challenge for clinicians to treat, specially bleeding in areas where endoscopic operation is difficult, or in the case of diffuse bleeding, tumor bleeding, and recurrent bleeding. Herein, we proposed a novel treatment strategy using biomaterials to protect the wound and isolate the erosion of digestive tract contents to prevent arterial bleeding in advance. By introducing chitosan to construct multihydrogen-bonding and an electrostatic interaction system, we developed polyethyleneimine/polyacrylic acid/chitosan (PEI/PAA/CS) multifunctional hydrogel. The new hydrogel is characterized by ultrafast gelation, strong tissue adhesion, gastric acid resistance, burst resistance, biocompatibility, hemostasis, and tissue repair. The addition of CS significantly improved the tissue adhesion, biocompatibility, hemostasis, and tissue repair ability of the hydrogel. The PEI/PAA/CS hydrogel could adhere to the ulcer surface and form a protective layer on the wound to prevent arterial bleeding. Importantly, the PEI/PAA/CS hydrogel also has the ability to stop bleeding and promote wound repair, which has been demonstrated in a variety of hemorrhage models in rats and rabbits. All of these factors indicate that the PEI/PAA/CS hydrogel is a promising biomaterial for reducing the risk of ulcerative arterial bleeding.
研究领域:
生物材料
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生物医学工程
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止血
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组织工程
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
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2024
·
IF 13.3
Panxianzhi Ni,Dengbinpei Duan,Shuting Xiong,Meng Zhong,Can Huang,Jing Shan,Tun Yuan,Jie Liang,Yujiang Fan,Xingdong Zhang
Gastric ulcer (GU) is currently one of the most serious and common gastrointestinal diseases worldwide, severely affects the quality of life of patients. However, treatment strategies have limitations, such as drug safety and lack of repair specificity. In this work, we developed a polyethyleneimine/polyacrylic acid/lactose-modified chitosan (PPCL) hydrogel to repair gastric ulcers. This hydrogel was characterized by robust tissue adhesion, low swelling rate, gastric acid fluid resistance, potent antioxidants and good biocompatibility, which could act as a physical barrier to prevent gastric acid and digestive enzymes from further destroying ulcers. In vivo data from a rat GU model confirmed that this hydrogel effectively promoted gastric ulcer healing, and the therapeutic effect was better than that of commercial gastric mucosal protectants. Further mechanistic research identified that the hydrogel accelerated GU healing by alleviating oxidative stress, promoting reepithelization, angiogenesis and mucosal repair. Our findings revealed that the PPCL hydrogel provides a promising therapeutic approach for healing gastric ulcer.
研究领域:
药理学
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材料科学
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生物医学工程
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胃肠病学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
·
IF 7.7
Shuxin Wang,Chenxia Guan,Pu Wang,Donghui Wang,Hanqi Wang,Ryan Chak Sang Yip,Hao Chen
Probiotics offer numerous beneficial functions for human bodies, while the low survival rate under gastric acid and short retention time in the intestine are the major obstacles to their utilization. To address these issues, we designed a novel dual-network hydrogel microsphere that combines gastric acid resistance with enhanced mucoadhesion, aiming for the targeted delivery of probiotics. Thiolated oxidized guar gum (SOGG) was disulfide-linked to form the first network, and sodium alginate (SA) was cross-linked with Ca 2+ to form the second network. Under the protection of the interpenetrating dual network microspheres, a much higher viability of Lactobacillus rhamnosus (LGG) (8.73 log CFU/mL) was achieved in simulated gastric fluid, compared to the zero-survival rate of free LGG. Mucoadhesion tests showed that the adhesion rate of SOGG/SA microspheres to the intestinal mucosa was 1.75 times higher than that of thiol-free microspheres. In vivo studies revealed that LGG-loaded microspheres significantly enhanced intestinal barrier function, remodeled the gut microbiome, and alleviated DSS-induced colitis in mice. Overall, SOGG/SA microspheres provide an effective strategy to the challenges of probiotic reduction in the stomach and rapid expulsion from the intestines, enhancing their health benefits.
研究领域:
药学
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生物技术
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药物递送系统
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微生物学
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胃肠病学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
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2020
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IF 8.758
Yuhao Zhou,Liang Liu,Yue Cao,Shuangjiang Yu,Chaoliang He,Xuesi Chen
Oral insulin delivery has revolutionized diabetes treatment, but challenges including degradation in the gastrointestinal environment and low permeation across the intestinal epithelium remain. Herein, to overcome these barriers, we developed a novel biodegradable nanocomposite microsphere embedded with metal–organic framework (MOF) nanoparticles. An iron-based MOF nanoparticle (NP) (MIL-100) was first synthesized as a carrier with an insulin loading capacity of 35%. The insulin-loaded MIL-100 nanoparticles modified with sodium dodecyl sulfate (Ins@MIL100/SDS) promoted insulin permeation across Caco-2 monolayer models in vitro. To improve resistance to the gastric acid environment, Ins@MIL100/SDS nanoparticles were embedded into a biodegradable microsphere to construct the nanocomposite delivery system (Ins@MIL100/SDS@MS). The microspheres effectively protected the MOF NPs from rapid degradation under acidic conditions and could release insulin-loaded MOF NPs in the simulated intestinal fluid. After the oral administration of Ins@MIL100/SDS@MS into BALB/c nude mice, increased intestinal absorption of the insulin was detected compared to the oral administration of free insulin or Ins@MIL100/SDS. Furthermore, significantly enhanced plasma insulin levels were obtained for over 6 h after oral administration of Ins@MIL100/SDS@MS into diabetic rats, leading to a remarkably enhanced effect in lowering blood glucose level with a relative pharmacological availability of 7.8%. Thus, the MOF-nanoparticle-incorporated microsphere may provide a new strategy for effective oral protein delivery.
研究领域:
药学
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纳米技术
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药物递送系统
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糖尿病治疗
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
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2024
·
IF 6
Wenyu Cui,Jin Liu,Lin Zhu,Mandlaa,Ziyu Sun,Zhongjun Chen
In recent years, the wine industry has demonstrated an increasing preference of fruits having high acidity. To enhance fermentation capacity and improve the quality of fruit wines, it is crucial to select Saccharomyces cerevisiae that are resistant to acidity and understand their mechanisms of acid resistance. The present study employed mutagenesis to obtain the wine yeast strain S . cerevisiae HJ-U17, which demonstrated favorable fermentation during the processing of high acidity sea buckthorn raw materials. The acid resistance mechanism underlying its performance was elucidated through comprehensive analysis encompassing cellular physiology and omics approaches. Our findings revealed a positive correlation between acid tolerance and factors such as cell membrane integrity, unsaturated fatty acid content, superoxide dismutase activity, and catalase activity. Conversely, acid tolerance showed a negative correlation with reactive oxygen species content. Transcriptomic data analysis unveiled enhancements in energy metabolism, fatty acid chain elongation, synthesis of palmitoleic acid and oleic acid, amino acid metabolism, oxidative stress response, and cell wall remodeling as the level of acid tolerance improved. Additionally, the participation of autophagy further augmented the strain's ability to withstand acidity. This study laid a foundation for the application of acid-resistant S . cerevisiae as the starter of fruit wine with high acidity.
研究领域:
微生物学
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发酵技术
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分子生物学
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基因组学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）