CELLULOSE
·
2022
·Vol. 29
·期 4
·2479-2495
·
IF 6.123
Tang Ruihua,Li Min,Yan Xueyan,Xie Mingyue,Liu Li Na,Li Zedong,Xu Feng
Cheap, rapid, simple and equipment-free nucleic acid extraction (NAE) is highly preferred for implementing nucleic acid detection at point-of-care (POC). Paper-based NAE materials have been extensively utilized due to their low cost, abundance, portability, biocompatibility and ease of chemical modification. However, it is challenging for users to choose the proper one from existing paper-based NAE materials for specific POC applications, which is determined by their physical and chemical properties. Additionally, building the relationship between the physical and chemical properties and the NAE efficiency of paper-based materials is instructive for development of new paper-based NAE materials. In this study, we first systematically compared the physical and chemical properties of six widely used paper-based NAE materials (namely Whatman filter paper #1, FTA card, FTA elute card, Fusion 5, silica membrane and polyethersulfone (PES) membrane), and then evaluated their NAE efficiency. The obtained results indicated that pore uniformity, wet strength, porosity and functional groups are key parameters to affect the efficiency of NAE. The NAE performance of FTA card is the best with high concentration and purity. Finally, we envision that more cost-effective paper-based NAE materials will be developed for POCT application in the future. Graphic abstract
研究领域:
生物技术
·
医学检验
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
·
2021
·Vol. 57
·期 1
·78-91
·
IF 3.713
Xiangna Chang,Xuefeng Chen,Pin Gong,Wenjuan Yang,Lan Wang,Ning Liu,Yao Su,Yuanyuan Zhao
Summary The anti-fatigue effects and structure-activity relationship of apple polysaccharides (APPs), the active components in apple pomace, remain unknown. We examined the effects of different extraction methods on polysaccharide structures and anti-fatigue activities. Additionally, the structural and biochemical properties of APPs prepared under acidic (ACAPP) or alkaline (ALAPP) conditions, were characterised. ACAPP and ALAPP were acidic heteropolysaccharides primarily comprising arabinose, rhamnose, galactose, glucose, xylose and galacturonic acid. The molecular weights of ACAPP and ALAPP were 110 kDa and 89.6 kDa, respectively. ALAPP had more branched chains than ACAPP and showed stronger free radical-scavenging and anti-fatigue properties. The anti-fatigue properties of APPs correlated with their anti-oxidant activities and were exerted through eliminating free radicals and mediating an oxidative balance. The stronger anti-fatigue activity of ALAPP is probably related to its structural characteristics. Collectively, our study provides a fundamental basis for the development and use of APPs in medicine and functional foods.
研究领域:
食品科学
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生物化学
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DOI
高影响因子
Food Bioscience
·
2024
·
IF 4.8
Qianni Chen,Lianzhu Lin,Mouming Zhao
Fucoidan possesses a variety of biological activities beneficial for human wellness. It is a good candidate for ameliorating obesity and diabetes. However, high-purity fucoidan products are limited due to the co-extraction of impurities i.e. alginate, protein and small molecular substances. In this study, the optimal preparation strategy for high-purity fucoidan from a template brown alga ( Saccharina japonica ) was screened out by the exploration of key and auxiliary steps. The key step was confirmed as moderate-temperature acid extraction and the auxiliary steps were fixed on ethanol refluxing and ball milling pretreatment. The fucose content of S. japonica fucoidan increased by 7.15 times and the sulfate group content increased by 4.87 times compared with those of fucoidans prepared by classic high-temperature acid extraction. Ball milling-assisted moderate-temperature acid extraction performed great effectiveness and universality in the extraction of high-purity fucoidans derived from four brown algae including S. japonica , Undaria pinnatifida, Sargassum fusiforme, and Fucus vesiculosus . Glycolipid-lowering activities of four fucoidans were confirmed by their interactions with intestinal bile acid, cholesterol micellar, glucose and probiotics including butyrate-producing bacteria, Bacteroides , Bifidobacterium , and Lactobacillus . Our study provided an efficient and universal extraction process for high-purity fucoidan with glycolipid-lowering activity from typical brown algae.
研究领域:
海洋生物学
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食品科学
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生物化学
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DOI
高影响因子
MICROCHEMICAL JOURNAL
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2022
·
IF 5.304
Meichao Liao,Yang Zhao,Xiaoyue Yang,Lin Yang,E-Hu Liu,Bin Lu,Jiye Wang,Xiaopeng Liu,Yanzhong Chang,Li Duan
The green and efficient extraction of bioactive compounds from plant biomass is an extraordinary area of interest in the pharmaceutical industries. In a perspective of sustainable development, we investigate an eco-friendly route using recycle hydrophobic deep eutectic solvent-assisted water extraction (RHD-WE) to effectively and simultaneously extract five main phenolic acids (caftaric acid, chlorogenic acid, caffeic acid, cichoric acid, 3,5-di-O-caffeoylquinic acid) from medicinal plant. In the procedure, 20 mg Taraxacum mongolicum powder was mixed with 800 µL water and 200 µL camphor/p-chlorophenol-based hydrophobic deep eutectic solvent (DES) at a molar ratio of 1:3. After 10 min vortex and centrifugation, the mixture was separated into aqueous phase and DES phase. Compared with other published procedures, phenolic acids were enriched in the aqueous phase. Aqueous solution of phenolic acid could be directly used as raw materials without further purification in pharmaceutical industries. Besides, hydrophobic DES phase could easily be recovered and reused in recycling studies. This is the first study of the recyclability of hydrophobic DES. Generally speaking, the model for the extraction of phenolic acids was well established and verified. The extraction efficiency was higher than other traditional extraction methods, approximately 14 mg/g of phenolic acids were obtained in the aqueous phase. In addition, the extraction mechanism was preliminarily discussed in this research. Such novel RHD-WE method might improve the existing schemes for extraction of bioactive compounds from plant materials. This study suggested that hydrophobic DESs could be used as green and safe auxiliary extractant for pharmaceutical applications.
研究领域:
植物化学
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药物开发
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可持续发展
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DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2023
·
IF 7.4
Haiying Shen,Lianhua Dong,Yunhua Gao,Xia Wang,Xinhua Dai
In this study, we designed a highly integrated microfluidic chip for nucleic acid extraction, amplification, and detection. Magnetic beads, which are used to capture nucleic acids on the chip, are trapped in the microwell arrays in a one-well-one-bead manner after local surface modification of the inner faces of the microwells. On-chip liquid introduction, delivery, and mixing are all carried out manually with one syringe and no other equipment. A hand-held device with precise temperature control and high-quality imaging is developed, which is only 2.3 cubic decimeters in volume and 1.2 kg in weight. Via the use of the Internet for wireless communication, the experiment and data analysis after inserting the chip into the device can be conducted by a smartphone anywhere there is an Internet connection. We carried out reverse transcription loop-mediated isothermal amplification (RT-LAMP) on the chip with the hand-held device. SARS-CoV-2 pseudoviruses are extracted, reverse transcribed, amplified, and detected on the chip with the hand-held device with satisfactory results. Thus, a highly integrated, easy-to-operate, and rapid nucleic acid detection microfluidic chip with a hand-held smartphone-controlled device is proposed, and this new platform for nucleic acid detection shows great potential for mobile point-of-care testing (POCT).
研究领域:
生物医学工程
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分子诊断
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移动医疗
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产品关联
DOI
PubMed
高影响因子
Nanoscale Advances
·
2025
·
IF 4.6
Sihua Qian,Yiting Wang,Junjie Fan,Tong Kong,Yuhui Wang,Kaizhe Wang,Yufeng Liao,Li Wang,Jianping Zheng
Exploring novel synthesis strategies for magnetic beads to extract nucleic acids is of great significance in the field of in vitro diagnostics. In the present research, monodisperse magnetic mesoporous silica beads were synthesized via the thermolysis reaction of Fe(acac)3 by using large-pore dendritic silica colloids as templates, and were further functionalized with a highly pH-sensitive histidine-glutamate co-oligopeptide for deoxyribonucleic acid extraction. The large-pore dendritic silica colloid scaffolds were utilized for high-density incorporation of superparamagnetic iron oxide nanoparticles within the vertical channels. The morphology and properties of the as-prepared pH-sensitive oligopeptide magnetic mesoporous silica beads were evaluated by transmission electron microscopy, scanning electron microscopy, vibrating sample magnetometry, X-ray photoelectron spectroscopy, X-ray diffraction testing and so on. The average size of the obtained magnetic beads was 370 nm in diameter with a narrow size distribution. The saturation magnetization and magnetic content of the resultant magnetic beads were 25 emu g−1 and 59%, respectively. Moreover, the magnetic mesoporous silica beads exhibited an obvious pH-responsive behavior. Due to these remarkable features, successful deoxyribonucleic acid capture using the as-prepared pH-sensitive oligopeptide magnetic mesoporous silica beads was achieved.
研究领域:
生物化学
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材料科学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
·
2021
·Vol. 56
·期 10
·5220-5229
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IF 3.713
Ying-Xing Huang,Jun-Ru Qi,Jin-song Liao,Wen-Xin Jiang,Yong Cao,Jie Xiao,Xiao-Quan Yang
Summary The objective of this work was to extract high methoxyl (HM) pectin with oxalic acid, characterise its physicochemical properties and evaluate its dispersion stability in acidified milk drink (AMD). HPSEC–MALLS analysis revealed that the obtained HM pectin was high in molecular weight (522.4 kDa). The ζ-potential of the obtained HM pectin in aqueous solution at pH 4.0 was −31.5 mV. The AFM images proved that the obtained HM pectin contained several long linear molecules. The apparent diameter of AMD stabilised by the obtained HM pectin was 636 nm at the concentration of 3.0‰. The stability analysis of AMD and the microscopic observation with confocal laser scanning microscopy (CLSM) proved that the obtained HM pectin was a prominent stabiliser in AMD.
研究领域:
食品科学
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化学工程
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产品关联
DOI
高影响因子
TALANTA
·
2022
·
IF 6.556
Siwenjie Qian,Yanju Chen,Cheng Peng,Xiaofu Wang,Hui Wu,Yang Che,Huanying Wang,Junfeng Xu,Jian Wu
African swine fever virus (ASFV) can cause highly contagious and fatal disease among domestic pigs, resulting in considerable economic losses for swine breeders. There is a strong demand for accurate, rapid, and simple detection methods especially for on-site application. Nucleic acid testing is the most commonly used method for ASFVdetection. However, traditional nucleic acid purification step is time- and labor-consuming. The nucleic acid purification, amplification and amplicons detection rely on laboratory settings which limits the on-site detection. Here, we proposed a simple and cost-effective detection method that utilized filter paper to purify nucleic acids from swine blood and employed CRISPR/Cas12a-mediated loop-mediated isothermal amplification (LAMP) reaction to detect ASFV. The filter paper which was made into dipsticks could effectively purify nucleic acids from whole blood in 2 min. This simple and low-cost purification method avoided multiple pipetting steps and potential amplification inhibitors (e.g., ethanol) that were generally used in traditional nucleic acids extraction processes. After nucleic acid purification, the lyophilized LAMP reagent dissolved by elution solution was employed to perform isothermal amplification reaction on a portable heating block. The CRISPR/Cas12a system was designed to specifically detect amplicons. Assisted by a portable homemade device, the fluorescent signals produced by positive samples could be observed by the naked eye, while negative samples remained colorless. The whole detection procedure could be finished within 50 min with a detection limit of one copies/μL. This established method provided a novel strategy for rapid visualized detection and showed great potential for on-site application.
研究领域:
兽医学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
MINERALS ENGINEERING
·
2020
·
IF 3.795
Zhigang Zhao,Peng Chen,Fan Yang,Qiuxia Liao,Yang Zhang,Panpan Zhao,Wanghuan Guo,Ruixi Bai,Chunqing Cai
The type of diglycolamic acid extractant N , N -di-2-ethylhexyldiglycolamic acid (D2EHDGAA) was examined for selective extraction and separation of Au(III) in acidic chloride solutions containing Pd(II), Pt(IV), Rh(III), and Cu(II). The separation factor β Au/Pt = 959.3 was observed in 7 M hydrochloric acid. The extraction of Au(III) was quick, and extraction equilibrium was reached in less than 5 min. The n -dodecane and kerosene diluents had a notable effect on the extraction of Au(III) with the D2EHDGAA, and the D2EHDGAA had excellent capacity in n -dodecane and kerosene. However, the selectivity coefficient of Au(III) and Pd(II) was better in n -dodecane (β Au/Pd = 1070) than in kerosene (β Au / Pd < 0.1) of 4 M hydrochloric acid. The extraction mechanisms of the Au(III) and Pt(IV) were investigated, and the results suggest that one metals ion is extracted with two and one extractants in 1 M HCl concentration. The D2EHDGAA-based n -dodecane extracting phase was easily activated using a low-concentration thiourea aqueous solution (0.01 M). The recyclability of D2EHDGAA for five cycles was investigated, and its efficiency decreased less than 4%.
研究领域:
化学工程
·
环境科学
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DOI
高影响因子
Chemistry-An Asian Journal
·
2019
·Vol. 14
·期 14
·2503-2511
·
IF 3.698
Ming Luo,Yuzhou Jiang,Jingbei Su,Zhuoyi Deng,Fangzhi Mou,Leilei Xu,Jianguo Guan
Graphical A good catch : A simple, versatile and efficient approach to extract nucleic acids in microsystems was demonstrated by developing cationic branched polyethyleneimine (PEI)-functionalized tubular micromotors. The PEI-functionalized tubular micromotor can selectively capture nucleic acids from an acid medium and recover the captured nucleic acids in a basic medium. Extraction of nucleic acids in microsystems is of significance for biomedical applications, but the current extraction methods generally require sophisticated microchannels and external equipment, hindering their practical applications. In this work, we have demonstrated a simple, versatile and efficient approach to extract nucleic acids in microsystems by developing cationic branched polyethyleneimine (PEI)-functionalized tubular micromotors. The as-developed tubular micromotors are fabricated by a two-step process combining the template-assisted electrodeposition and carbodiimide chemistry, and contain an inner catalytic Pt layer, a middle magnetic Ni layer and an outer cationic PEI layer. They exhibit autonomous bubble-propelled motion in aqueous hydrogen peroxide solutions, which can be guided by an external magnetic field, and the surface charges can be reversibly modulated by changing the pH value of the solution. Consequently, the as-developed tubular micromotors can selectively absorb nucleic acids from acidic solutions and desorb them into alkaline solutions, leading to the extraction of nucleic acids with high efficiency without external stirring. Furthermore, they can be operated in a microchannel chip without the aid of a pumping system. Our results indicate that this PEI-functionalized tubular micromotor platform provides a novel, simple and versatile microsystem nucleic acid extraction technology, holding considerable promise for important practical applications.
研究领域:
生物医学工程
·
分析化学
·
纳米技术
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产品关联
DOI
PubMed
高影响因子
Micromachines
·
2025
·Vol. 16
·期 5
·581
·
IF 3
Zhuo Gao,Pan Wang,Chang Chen,Jian Duan,Shilun Feng,Bo Liu
The increasing spread of infectious diseases caused by pathogenic microorganisms underscores the urgent need for highly sensitive, portable, and rapid nucleic acid detection technologies to facilitate early diagnosis and effective prevention. In this study, we developed a fluorescence-based nucleic acid detection platform that integrates a microfluidic chip with an all-inorganic perovskite photodetector. The system enables integrated operation of nucleic acid extraction, purification, and amplification on a microfluidic chip, combined with real-time electrical signal readout via a CsPbBr3perovskite photodetector. Experimental results indicate that the photodetector exhibits high responsivity at 530 nm, aligning well with the primary emission peak of FAM. The system demonstrates a strong linear correlation between photocurrent and FAM concentration over the range of 0.01–0.4 μM (R2= 0.928), with a low detection limit of 0.01 μM and excellent reproducibility across multiple measurements. Validation using FAM standard solutions and Escherichia coli samples confirmed the system’s reliable linearity and signal stability. This platform demonstrates strong potential for rapid pathogen screening and point-of-care diagnostic applications.
研究领域:
生物技术
·
医学诊断
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产品关联
DOI
PubMed
高影响因子
DESALINATION
·
2024
·
IF 8.3
Ruzhen Zhao,Manxing Huo,Qifeng Wei,Xiulian Ren,Yongming Zhu
Efficient extraction of lithium and boron resources from salt-lakes is a prominent topic of research among scholars. In this study, a process route for the simultaneous extraction of lithium and boric acid was employed, followed by their separation through stepwise stripping. During the experiments, a coupling effect in the extraction of Li + and H 3 BO 3 was found, leading to an in-depth investigation of their mutual promotion mechanisms. Three significant patterns have been clearly verified through experiments, molecular dynamics simulations and quantum chemical calculations: First, the acidic extractant N221 can not only extract Li + , but also enhance the extraction of H 3 BO 3 ; Second, mono-fatty alcohol R-OH as dilute can effectively extract H 3 BO 3 while also promoting the extraction of Li + . Lastly, Li + and H 3 BO 3 can mutually promote each other's extraction. Using the Qinghai old brine, N221 system achieved a 99 % extraction efficiency for H 3 BO 3 , with an initial lithium extraction of 97 %, around 80 % in subsequent rounds. This paper proposes an effective method for the extraction of Li + and boric acid, and thoroughly studied the mechanism of their mutual promotion of extraction. This research offers new insights into the comprehensive utilization of salt-lake resources.
研究领域:
化学工程
·
材料科学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）