BIORESOURCE TECHNOLOGY
·
2015
·
IF 4.494
Binzhe Sun,Gege Peng,Lian Duan,Aihua Xu,Xiaoxia Li
A simple pretreatment method, NaOH swelling at a low temperature and then HCl regeneration, was developed for depolymerization of cellulose to glucose. Cellulose was transformed into amorphous hydrogel during the treatment; and due to the easy diffusion of H + and Cl − ions into the cellulose hydrogel network as well as the strong ability of Cl − to disrupt the massive hydrogen bond, the hydrolysis rate was greatly enhanced. The method is effective for α-cellulose, microcrystalline cellulose, filter paper, ramie fiber and absorbent cotton. Even at a high α-cellulose concentration of 50 g/L, 29.1% yield of glucose was still obtained within 10 min at 160 °C under microwave irradiation, where up to 16.2 g/L of glucose solution was given. The influence of NaOH concentration, HCl concentration, hydrolysis temperature and time on the hydrolysis rate was investigated. The structure of cellulose hydrogel was also studied to confirm the reaction mechanism.
研究领域:
生物化学
·
可再生能源
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CONTROL
·
2021
·
IF 5.548
Yaqi Hou,Jiao Liu,Yanchun Shao,Xitian Peng,Dawen Zhang,Lifang Hu,Fusheng Chen,Youxiang Zhou
Modified mycotoxins can cause an underestimation of exposure risk due to their untraceable characteristics. Hongqu , a traditional Chinese fermented product, is widely used as food colorants , fermenters and functional foods for about 2000 years. Though there were several methods to reduce citrinin (CIT) content in Hongqu , the risk assessment and prevention of modified CIT remain inadequate, causing potential risk to human health. The current study firstly identified the occurrence of modified CIT in Hongqu via acid, alkaline and enzyme hydrolysis combined with chromatographic and mass spectrometric approaches. Then 8 Hongqu samples and 5 Monascus products were treated using the optimized acid hydrolysis method and analyzed by ultra-high-performance liquid chromatography with fluorescence detector. The results showed that CIT content in the CIT positive Hongqu increased by 35.28%–458.13% after acid hydrolysis, demonstrating there could be a underestimation risk in the CIT positive Hongqu samples, and the modification of CIT might not be chemical modified, but matrix associated.
研究领域:
食品安全
·
微生物学
·
毒理学
✓ 已验证
产品关联
DOI
高影响因子
Journal of Environmental Chemical Engineering
·
2023
·
IF 7.7
Haoyue Tang,Yu Zhang,You Wu,Wenyu Wu,Shiying Bi,Junjie Wu,Shitong Yang
Turning agricultural wastes into green adsorbents is a compatible and cost-effective strategy for solid waste disposal and environmental pollution control. Herein, the discarded orange peel (OP) sample was purified via coupled alkali-acid hydrolysis and the obtained adsorbents were applied for eliminating toxic cadmium (Cd(II)) from the liquid phase. Based on the single factor experiments, the optimal Cd(II)-capturing adsorbent (i.e., OP-OH-H-25) was generated under the purification condition of soaking raw OP in 2.0 M NaOH solution at 25 °C followed by an acid hydrolysis treatment with 2.5 M HCl solution at 25 °C. Spectral analyses reflected the efficient elution of hemicellulose/pectin and lignin impurities, the transformation of cellulose type I to cellulose type II, and the exposure of sulfhydryl functional groups. The kinetics removal process of Cd(II) by OP-OH-H-25 reached equilibrium after 0.5 h with a removal efficiency of ∼88%. The maximum Cd(II) sorption capacity of OP-OH-H-25 (i.e., 124.90 mg/g) was higher than a series of previously reported adsorbents. The sorption behavior of Cd(II) onto the prepared OP-OH-H-25 material was mostly interfered with by the coexisting Mg 2+ , Ca 2+ , and heavy metals. The desorption data and spectroscopic analysis jointly pointed to the inner-sphere binding of Cd(II) with OP-OH-H-25 functional groups. Overall, the extracted OP-OH-H-25 sample could act as a promising decontaminant for the remediation of Cd(II)-polluted water environment.
研究领域:
环境科学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2018
·
IF 4.946
Dandan Li,Yao Zhang,Na Yang,Zhengyu Jin,Xueming Xu
This study aimed to improve induced electric field (IEF)-assisted hydrolysis of polysaccharide by controlling electrical conductivity. As the conductivity of reaction medium was increased, the energy efficiency of IEF was increased because of deceased impedance, as well as enhanced output voltage and temperature, thus the hydrolysis of guar gum (GG) was accelerated under IEF. Changes in weight-average molecular weight (Mw) suggested that IEF-assisted hydrolysis of GG could be described by the first-order kinetics 1/Mw ∝ k t, with the rate constant ( k ), varying directly with the medium conductivity. Although IEF-assisted hydrolysis largely disrupted the morphological structure of GG, it had no impact on the chemical structure. In comparison to native GG, the steady shear viscosity of hydrolyzed GG dramatically declined while the thermal stability slightly decreased. This study extended the knowledge of electrical conductivity upon IEF-assisted acid hydrolysis of GG and might contribute to a better utilization of IEF for polysaccharide modification.
研究领域:
食品科学
·
生物化学
✓ 已验证
DOI
高影响因子
Journal of Materials Chemistry B
·
2019
·Vol. 7
·期 9
·1450-1459
·
IF 5.047
Yanlei Hu,Juan Yu,Liang Liu,Yimin Fan
Direct extraction of silk nanofibers (SNs) from natural silk fibers was developed via a low-intensity ultrasonic-assisted sulfuric acid hydrolysis process. The effects of sulfuric acid (H2SO4) amount, hydrolysis time and temperature were investigated. SNs with a length and width of 306 ± 107 nm and 4–18 nm, respectively, were obtained with a yield of 64.54%. Furthermore, unlike for the silk nanofibers formed from a regeneration process, the original crystal structure of the silk was maintained. The aqueous dispersion of SNs was steady for at least 30 days at pH 3 and 7–10 with no aggregation. Furthermore, the as-prepared SNs were amphoteric due to the intrinsic characteristics of the protein that endow them with the ability to mix well with either positively or negatively charged polymers, possibly contributing to the field of high-tech silk materials in the future.
研究领域:
材料科学
·
生物化学
✓ 已验证
DOI
PubMed
高影响因子
Food & Function
·
2019
·Vol. 10
·期 9
·5531-5543
·
IF 3.241
Zhiyu Fang,Yutao Chen,Ge Wang,Tao Feng,Meng Shen,Bin Xiao,Jingyi Gu,Weimin Wang,Jia Li,Yongjun Zhang
Caenorhabditis elegans is an important model organism for studying stress response mechanisms and identifying genetic pathways that influence longevity. The present study was designed to explore the in vivo-antioxidant potential and the probable mechanism of acid hydrolysates prepared from A. auricula polysaccharides (AAPHs-F) with the optimal acid hydrolysis conditions using Box–Behnken design, and C. elegans was used as a model organism. The effects of AAPHs-F on the locomotory behavior, lifespan, activities of antioxidant-related enzymes and levels of antioxidants in C. elegans were studied. In addition, the potential of AAPHs-F in up-regulating the expression of antioxidant-related genes in C. elegans, such as daf-16, skn-1, sod-1, sod-2 and sir-2.1, and the inhibition of cell apoptosis of C. elegans were also discussed. The results indicated that AAPHs-F could significantly increase the U-Turn frequency of nematodes, extend their lifespan, enhance antioxidant systems including superoxide dismutase (SOD) by 70.60%, catalase (CAT) by 73.45% and glutathione reductase (GR) by 258.68% (p < 0.01), increase the level of glutathione (GSH) by 110.22% (p < 0.01), and decrease the level of reactive oxygen species (ROS) and malondialdehyde (MDA) by 31.86% and 46.16% (p < 0.01), respectively. Quantitative real-time polymerase chain reaction (qRT-PCR) results showed that AAPHs-F could up-regulate mRNA expression levels of daf-16, skn-1, sir, sod-1 and sod-2 in wild-type C. elegans (>1.3 fold) when treated at a concentration of 0.1 mg mL−1 (p < 0.05 or p < 0.01). AAPHs-F was concluded to be heteropolysaccharides composed of mannose, glucose and galactose with a molar ratio of 12.7 : 3.25 : 1. The molecular weight of AAPHs-F was determined to be 885.37 Da. Furthermore, AAPHs-F is mainly formed of (1 → 3)-linked-α-D-glucopyranose, and carboxyl or acetamide is present in the molecule. In summary, our studies provide evidence that AAPHs-F helps improve the antioxidant defense system, and up-regulation of stress and longevity related genes suggests the possible involvement of these genes in the prevention of stress damage in C. elegans.
研究领域:
生物化学
·
分子生物学
·
遗传学
✓ 已验证
DOI
PubMed
高影响因子
CARBOHYDRATE RESEARCH
·
2017
·
IF 2.096
Jiaojiao Lu,Chunqing Ai,Li Guo,Yinghuan Fu,Chunyang Cao,Shuang Song
Because the glycosidic linkage of uronic acid is most resistant to acid, oligosaccharides may be formed during the acid hydrolysis of acidic polysaccharides . To take chondroitin sulfate (CS) as an example of acidic polysaccharides , the present study characterized the oligosaccharides released through acid hydrolysis and demonstrated their usefulness for structural confirmation. Acid hydrolysates of commercial standard CSs from shark cartilage and porcine bone were elucidated using HPLC-MS n after 1-phenyl-3-methyl-5-pyrazolone (PMP) derivatization , and altogether 11 di-, tri- and tetra-saccharides with or without sulfate/acetyl groups were identified by their multi-stage mass spectra. Meanwhile the trends of reaction yields of these oligosaccharides alone with trifluoroacetic acid (TFA) concentrations (0.1–2.0 M) were investigated, and 0.2 M TFA was recommended. Then three real samples, sturgeon backbone, porcine trachea and sea cucumber were analyzed, and their CSs were identified by detection of characteristic oligosaccharide fragments. The present study indicated that acid hydrolysis could provide information for acetyl substitution, sulfation and glycosidic linkages, and was helpful for the structural analysis of acidic polysaccharides.
研究领域:
生物化学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2024
·
IF 8.1
Wenbo Jiang,Yiwen Wei,Shilong Li,Chenye Dai,Can Yuan,Jian Lu,Dong Zou,Tianxiang Yu,Yuqing Sun,Wenheng Jing
Bio-heavy oil with high oxygen content and combustion calorific value has attracted lots of attention as a green and sustainable fuel to cope with the increasingly severe energy shortages. However, the high salt content limits its combustion utilization due to potential risks such as equipment corrosion and wear blockage. The traditional desalination method using sulfuric acid causes a large amount of wastewater, Herein, we propose an assisted metal-ion interphase mass transfer desalination strategy by preparing carbonated water-in-bio-heavy oil (CW/O) emulsions with continuous membrane emulsification. α-Al 2 O 3 membrane with an average pore size of 600 nm was selected as the emulsification medium. Saturated carbonic acid solutions as a dispersed phase were pressed through the membrane pores into bio-heavy oil to form uniform micron-sized CW/O emulsions. The prepared CW/O droplets provide a large interphase mass transfer interface which assists the hydrolysis of fatty acid salts of bio-heavy oil in the acidic environment and yields an efficient transfer of metal ions. As a result, the CW/O emulsion with an average droplet size (D 3,2 ) of 4.29 μm and a specific surface area of 139.63 m 2 /m 3 was prepared at a pressure of 0.5 MPa, achieving a salt removal efficiency of up to 85.62 %. This study provides a novel chemical-free and effective strategy for efficient oil product refining while utilizing CO 2 through the continuous preparation of micron-sized CW/O droplets with sufficient interphase mass transfer area.
研究领域:
化学工程
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
Polymers
·
2019
·Vol. 11
·期 7
·1130
·
IF 3.164
Dingyuan Zheng,Yangyang Zhang,Yunfeng Guo,Jinquan Yue
Herein, walnut shell (WS) was utilized as the raw material for the production of purified cellulose. The production technique involves multiple treatments, including alkaline treatment and bleaching. Furthermore, two nanocellulose materials were derived from WS by 2,2,6,6-tetramethylpiperidine-1-oxyl radical (TEMPO) oxidation and sulfuric acid hydrolysis, demonstrating the broad applicability and value of walnuts. The micromorphologies, crystalline structures, chemical functional groups, and thermal stabilities of the nanocellulose obtained via TEMPO oxidation and sulfuric acid hydrolysis (TNC and SNC, respectively) were comprehensively characterized. The TNC exhibited an irregular block structure, whereas the SNC was rectangular in shape, with a length of 55–82 nm and a width of 49–81 nm. These observations are expected to provide insight into the potential of utilizing WSs as the raw material for preparing nanocellulose, which could address the problems of the low-valued utilization of walnuts and pollution because of unused WSs.Keywords:Walnut shell;nanocellulose;TEMPO oxidation;sulfuric acid hydrolysis;ultrasonication
研究领域:
材料科学
·
农业废弃物利用
·
化学工程
✓ 已验证
DOI
PubMed
高影响因子
CHINESE JOURNAL OF POLYMER SCIENCE
·
2020
·Vol. 39
·期 3
·327-336
·
IF 3.154
Shi Zhen,Wang Qi,Li Gui-Fei,Shou Yu-Feng,Zong Hong-Jie,Yan Shi-Feng,Zhang Kun-Xi,Yin Jing-Bo
Self-healing hydrogels with the shear-thinning property are novel injectable materials and are superior to traditional injectable hydrogels. The self-healing hydrogels based on 2-ureido-4[1 H ]-pyrimidinone (UPy) have recently received extensive attention due to their dynamic reversibility of UPy dimerization. However, generally, UPy-based self-healing hydrogels exhibit poor stability, cannot degrade in vivo and can hardly be excreted from the body, which considerably limit their bio-application. Here, using poly (l -glutamic acid) (PLGA) as biodegradable matrix, branching α-hydroxy- ω -amino poly(ethylene oxide) (HAPEO) as bridging molecule to introduce UPy, and ethyl acrylate polyethylene glycol (MAPEG) to introduce double bond, the hydrogel precursors (PMHU) are prepared. A library of the self-healing hydrogels has been achieved with well self-healable and shear-thinning properties. With the increase of MAPEG grafting ratio, the storage modulus of the self-healing hydrogels decreases. The self-healing hydrogels are stable in solution only for 6 h, hard to meet the requirements of tissue regeneration. Consequently, ultraviolet (UV) photo-crosslinking is involved to obtain the dual crosslinking hydrogels with enhanced mechanical properties and stability. When MAPEG grafting ratio is 35.5%, the dual crosslinking hydrogels can maintain the shape in phosphate-buffered saline solution (PBS) for at least 8 days. Loading with adipose-derived stem cell spheroids, the self-healing hydrogels are injected and self-heal to a whole, and then they are crosslinked in situ via UV-irradiation, obtaining the dual crosslinking hydrogels/cell spheroids complex with cell viability of 86.7%±6.0%, which demonstrates excellent injectability, subcutaneous gelatinization, and biocompatibility of hydrogels as cell carriers. The novel PMHU hydrogels crosslinked by quadruple hydrogen bonding and then dual photo-crosslinking of double bond are expected to be applied for minimal invasive surgery or therapies in tissue engineering.
研究领域:
生物材料学
·
组织工程学
✓ 已验证
DOI
高影响因子
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
·
2016
·
IF 1.114
Xue-Fang Chen,Chao Huang,Lian Xiong,Bo Wang,Gao-Xiang Qi,Xiao-Qing Lin,Can Wang,Xin-De Chen
Elephant grass (Pennisetum purpureum) dilute acid hydrolysate contains 34.6 g/L total sugars. The potential of lipid production by oleaginous yeast Trichosporon cutaneum grown on elephant grass acid hydrolysate was investigated for the first time. During the fermentation process on the elephant grass acid hydrolysate, glucose, xylose, and arabinose could be well utilized as carbon sources by T. cutaneum. Interestingly, xylose was almost no use before glucose was consumed completely. This illustrated that simultaneous saccharification of xylose and glucose by T. cutaneum did not occur on elephant grass acid hydrolysate. The highest biomass, lipid content, lipid yield, and lipid coefficient of T. cutaneum were measured after the sixth day of fermentation and were 22.76 g/L, 24.0%, 5.46 g/L, and 16.1%, respectively. Therefore, elephant grass is a promising raw material for microbial oil production by T. cutaneum.
研究领域:
生物技术
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL PAPERS
·
2023
·Vol. 77
·期 10
·5999-6006
·
IF 2.2
Yuan Yifan,He Fuduo,Li Xue,Xu Dehua,Yan Zhengjuan,Wang Xinlong
Corncob has a high yield and contains a large number of cellulose substances. Wet phosphoric acid hydrolysis of corncob is of great significance to the utilization of organic waste, and provides theoretical support for the development of sugar-containing synergistic phosphorus fertilizers. In this study, the kinetics of hydrolysis of corncob by wet process phosphoric acid was investigated. The time evolution of total sugar, xylose and furfural formation during a 12-h hydrolysis was simulated by Saeman and biphasic models within differential acid concentrations and temperatures. The formations of both total sugar and furfural coincide a first-order irreversible reaction, which was not applicable to the Saeman model nor the biphasic model. Meanwhile, xylose formation was more suitable for the Saeman model. The optimal conditions for xylose are: a temperature of 100 °C, a reaction time of 720 min, an acid concentration of 55.6% (w/w). The xylose yield of corncobs was 0.15 g/g in the optimal conditions. Graphical abstract
研究领域:
生物化学
·
农业科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）