RSC Advances
·
2025
·Vol. 15
·期 7
·5413-5425
·
IF 4.6
Zhuo Li,Hui Li,Zhenrong Tang,Qingxia Tang,Chang Liao,Hua Tang,Dan Wang
Bacterial biofilms play a crucial role in the emergence of antibiotic resistance and the persistence of chronic infections. The challenge of effectively eradicating bacterial biofilms while ensuring minimal toxicity to normal cells persists. Carbon-based artificial nanoenzymes have attracted considerable attention as emerging nanotheranostic agents, owing to their biocompatibility, cost-effectiveness, and straightforward synthesis. In this study, we have developed a multifunctional carbon dots (CDs) system, specifically CDs functionalized with 1-(3-aminopropyl) imidazole (API), termed CDs-API. This system demonstrates acid-activated antibiofilm activity. The CDs-API were synthesized from chlorogenic acid (ChA), a bioactive compound naturally occurring in coffee, and subsequently functionalized with API to achieve charge-switchable properties under acidic conditions. This distinctive feature enables CDs-API to efficiently penetrate bacterial biofilms and selectively target the colonized bacteria. The enzyme-like activity of CDs-API effectively consumes high levels of glutathione (GSH) within the biofilm, leading to the accumulation of reactive oxygen species (ROS). Consequently, this process degrades the extracellular polymeric substance (EPS) matrix, damages bacterial DNA and protein structures, and disrupts the redox balance, ultimately leading to bacterial cell death. Experimental results demonstrated that CDs-API effectively inhibited the growth of methicillin-resistant Staphylococcus aureus (MRSA) and Pseudomonas aeruginosa (PAE) while promoting wound healing with minimal damage to healthy tissues. The acid-activated charge-switchable capability of CDs-API provides superior antibacterial efficacy compared to traditional antibiotics, rendering it a promising candidate for the treatment of bacterial biofilm infections.
研究领域:
纳米医学
·
微生物学
·
药理学
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产品关联
DOI
PubMed
高影响因子
Scientific Reports
·
2025
·Vol. 15
·期 1
·1-6
·
IF 3.9
Li Wenfang,Lu Yuze,Wang Jiajun,Li Chuanbi
In this study, a novel artificial enzyme Zn(II)-SMM with simple organic structure for PNPA-directed hydrolysis is proposed. The introduction of hydrophobic quinoline and dipyridinium in Zn(II)-SMM system demonstrates high activity for the complexation of Zn 2+ and hydrolysis of PNPA, and the catalytic rate (k cat /k uncat ) exceeds 5239 times that of non-catalytic systems. The newly designed small molecule organic complex effectively binds to and catalyzes the hydrolysis of substrate PNPA in accordance with Michaelis-Menten kinetics. The mechanism with activation by single Lewis acid provides fundamental insights for the development of small molecule hydrolases.
研究领域:
生物化学
·
分子生物学
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产品关联
DOI
PubMed
高影响因子
Journal of Nano Research
·
2017
·
IF 0.511
Cao Ming,Xiao Wan Song,Yu Jiao Zhang,Chang Zhi Xu,Peng Chen,Ru Xia,Tian Long Hu,Jia Sheng Qian,Ke Liang Wu
pH responsive polymeric nanoparticles have emerged as a promising technology platform for targeted and controlled drug delivery in recent years. In this paper, endosomal pH-activatable doxorubicin (DOX) and core-crosslinked polymeric nanoparticles (DCNPs) were prepared and investigated for potent growth inhibition of human cancer cells in vitro. In vitro drug release studies, DOX conjugated nanoparticles with hydrazone bond showed a pH sensitive release phenomenon, that is, the releasing is significantly faster at mildly acidic condition with pH of 5.5 than that at physiological condition. Confocal laser scanning microscope (CLSM) observations revealed that DOX conjugated nanoparticles delivered and released DOX into the cytosols as well as cell nuclei of Hela cells following 6 h incubation. MTT assays demonstrated that these pH-sensitive DOX nanoparticles exhibited high antitumor effect to HeLa cells. The conjugated DOX polymeric nanoparticles may be a promising candidate as a nanoscale and pH-sensitive drug delivery vehicle for cancer therapy.
研究领域:
生物医学工程
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药学
·
肿瘤学
✓ 已验证
DOI
高影响因子
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
·
2023
·
IF 5
Haimei Wu,Shuo Xu,Kaiwen Lin,Jin Xu,Datian Fu
An intelligent drug delivery system allows temporal, spatial and dose control of drug release, which is greatly important in cancer therapy. However, the low stability of the carrier and poor controllability of the anticancer drug may limit its biomedical application. To overcome these drawbacks, an acidity-activated dynamic halloysite nanotubes (HNTs) drug delivery system was designed to ensure efficient cancer therapy. HNTs are first modified with an aminosilane crosslinker, via (3-aminopropyl) trimethoxysilane (APTES). The latter was a linker to convert partial amino groups to hydrazone groups through stepwise organic transformations. Then, the anticancer drug, doxorubicin (DOX), is loaded through a covalent linkage to yield HNTs-NN-DOX. Finally, the soybean phospholipid (SP) is coated on the surface of HNTs-NN-DOX, named [email protected] to further enhance the stability and biocompatibility of HNTs. The acid-labile bond between the HNTs and DOX releases drugs immediately in response to the tumor microenvironment (TME). In vitro cell toxicity and imaging assay results indicated that the [email protected] has high cell cytotoxicity on 4T1 cells. We provide a new strategy for the biomedical application of HNTs in nanomedicine , which proved that HNTs selective to slight pH differences and promote release of cytotoxic compounds.
研究领域:
药学
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纳米医学
·
肿瘤学
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产品关联
DOI
高影响因子
PeerJ
·
2017
·
IF 2.177
Jia Yao,Yinyun Ma,Wei Zhang,Li Li,Yun Zhang,Li Zhang,Hui Liu,Jingman Ni,Rui Wang
TH(AGYLLGHINLHHLAHL(Aib)HHIL-NH 2 ), a histidine-rich, cell-penetrating peptide with acid-activated pH response, designed and synthesized by our group, can effectively target tumor tissues with an acidic extracellular environment. Since the protonating effect of histidine plays a critical role in the acid-activated, cell-penetrating ability of TH, we designed a series of new histidine substituents by introducing electron donating groups (Ethyl, Isopropyl, Butyl) to the C-2 position of histidine. This resulted in an enhanced pH-response and improved the application of TH in tumor-targeted delivery systems. The substituents were further utilized to form the corresponding TH analogs (Ethyl-TH, Isopropyl-TH and Butyl-TH), making them easier to protonate for positive charge in acidic tumor microenvironments. The pH-dependent cellular uptake efficiencies of new TH analogs were further evaluated using flow cytometry and confocal laser scanning microscopy, demonstrating that ethyl-TH and butyl-TH had an optimal pH-response in an acidic environment. Importantly, the new TH analogs exhibited relatively lower toxicity than TH. In addition, these new TH analogs were linked to the antitumor drug camptothecin (CPT), while butyl-TH modified conjugate presented a remarkably stronger pH-dependent cytotoxicity to cancer cells than TH and the other conjugates. In short, our work opens a new avenue for the development of improved acid-activated, cell-penetrating peptides as efficient anticancer drug delivery vectors.
研究领域:
生物化学
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肿瘤学
·
药物递送系统
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DOI
PubMed
高影响因子
Matter
·
2025
·
IF 17.5
Bingzhe Wang,Qilong Li,Yang Du,Qiyue Wang,Zeyu Liang,Mengmeng Li,Canyu Huang,Li Yao,Guangyuan Shi,Jie Tian,Fangyuan Li,Daishun Ling
Magnetic particle imaging (MPI) holds immense promise as a non-invasive biomedical imaging modality, but its advancement is hindered by the absence of activatable probes for biomarker-specific imaging. Here, we introduce a ligand-mediated acid-activatable MPI probe (LAMP) composed of magnetic nanoparticles linked via acid-sensitive imine bond-containing ligands. The LAMP exhibits stable assembly in neutral conditions and rapid disassembly in acidic microenvironments. The imine bond crosslinking promotes a compact structure that enhances magnetic dipole interactions, significantly quenching the initial MPI signal. Upon exposure to acidic conditions, the probe disassembles, restoring the MPI signal in a highly controlled, environment-dependent manner. This switchable assembly enables precise, real-time imaging of acidity-associated diseases such as sepsis progression. Our findings demonstrate that the ligand-mediated modulation of magnetic dipole interactions provides a versatile platform for the design of next-generation MPI probes for biomarker-specific MPI imaging.
研究领域:
医学影像
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纳米医学
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分子生物学
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产品关联
DOI
高影响因子
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
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2025
·Vol. 36
·期 11
·1-22
·
IF 2.8
Liu Gang,Zhang Aoyang,Ahmed Shams Forruque,Ma Weiwu
In the current study, nitrogen and oxygen (N/O)-co-doped porous carbon for supercapacitors was successfully synthesized from sunflower discs through activation modification with potassium citrate and urea. Material characterization and electrochemical performance testing in three-electrode and two-electrode systems demonstrated that the porous carbon (SDU-900), prepared under activation conditions at 900 \(^{\circ }\) C exhibited an interconnected pore structure and excellent electrochemical properties. In a three-electrode system (6 M KOH as the electrolyte, current density of 0.5 A/g), SDU-900 demonstrated a high specific capacitance of 294.2 F/g. Moreover, the symmetric device based on SDU-900 exhibits an excellent energy density of 20.05 Wh/kg at a power density of 495 W/kg. After 15,000 galvanostatic charge–discharge (GCD) cycles at 5 A/g, the initial specific capacitance was retained at 93.89%, indicating remarkable cycling stability. Notably, the activation process utilized sunflower discs discarded from agricultural production as precursors, replacing the highly corrosive alkali activators with potassium citrate, a benign salt. This approach provides an environmentally friendly method for synthesizing porous carbon for supercapacitor applications.
研究领域:
材料科学
·
环境科学
·
能源研究
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DOI
高影响因子
MOLECULAR PHARMACEUTICS
·
2017
·
IF 4.44
Xiaoxiao Shi,Meili Hou,Shuang Bai,Xiaoqian Ma,Yong-E Gao,Bo Xiao,Peng Xue,Yuejun Kang,Zhigang Xu,Chang Ming Li
Stimuli-responsive nanomedicine with theranostic functionalities with reduced side-effects has attracted growing attention, although there are some major obstacles to overcome before clinical applications. Herein, we present an acid-activatable theranostic unimolecular micelles based on amphiphilic star-like polymeric prodrug to systematically address typical existing issues. This smart polymeric prodrug has a preferable size of about 35 nm and strong micellar stability in aqueous solution, which is beneficial to long-term blood circulation and efficient extravasation from tumoral vessels. Remarkably, the polymeric prodrug has a high drug loading rate up to 53.1 wt%, which induces considerably higher cytotoxicity against tumor cells (HeLa cells and MCF-7 cells) than normal cells (HUVEC cells) suggesting a spontaneous tumor-specific targeting capability. Moreover, the polymeric prodrug can serve as a fluorescent nanoprobe activated by the acidic microenvironment in tumor cells, which can be used as a promising platform for tumor diagnosis. The superior antitumor effect in this in vitro study demonstrates the potential of this prodrug as a promising platform for drug delivery and cancer therapy.
研究领域:
纳米医学
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癌症治疗
·
药物递送系统
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产品关联
DOI
PubMed
高影响因子
Materials
·
2024
·Vol. 17
·期 5
·1118
·
IF 3.1
Hanyun Xie,Runhua Chen,Yuxia Song,Yan Shen,Fengming Song,Bo He,Xiaomei Jiang,Yifan Yin,Wenming Wang
Transition metals and their oxide compounds exhibit excellent chemical reactivity; however, their easy agglomeration and high cost limit their catalysis applications. In this study, an interpolation structure of a Myriophyllum verticillatum L. biochar-supported Mn/Mg composite (Mn/Mg@MV) was prepared to degrade triphenyl phosphate (TPhP) from wastewater through the activating periodate (PI) process. Interestingly, the Mn/Mg@MV composite showed strong radical self-producing capacities. The Mn/Mg@MV system degraded 93.34% TPhP (pH 5, 10 μM) within 150 min. The experimental results confirmed that the predominant role of IO3· and the auxiliary ·OH jointly contributed to the TPhP degradation. In addition, the TPhP pollutants were degraded to various intermediates and subsequent Mg mineral phase mineralization via mechanisms like interfacial processes and radical oxidation. DFT theoretical calculations further indicated that the synergy between Mn and Mg induced the charge transfer of the carbon-based surface, leading to the formation of an ·OH radical-enriched surface and enhancing the multivariate interface process of ·OH, IO3, and Mn(VII) to TPhP degradation, resulting in the further formation of Mg PO4mineralization.
研究领域:
环境科学
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材料科学
·
化学工程
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产品关联
DOI
PubMed
高影响因子
Materials Science & Engineering C-Materials for Biological Applications
·
2021
·
IF 7.328
Meizhe Yu,Gaoke Zhang,Peili Li,Haojie Lu,Wentao Tang,Xu Yang,Ruobing Huang,Fan Yu,Wenzhen Wu,Yuhong Xiao,Xiaodong Xing
Many medical and chemical applications require the precise supply of antimicrobial components in a controlled manner at the location of mature biofilm deposits. This work reports a facile strategy to fabricate nanoscale metal-organic frameworks (NMOFs) coencapsulating the antibacterial ligand (lysine carbon dots, Lys-CDs) and targeted drug (folic acid, FA) in one pot to improve antibiofilm efficiency against established biofilms. The resulting products are characterized by transmission electron microscopy, field-emission scanning electron microscopy, powder x-ray diffraction, and ultraviolet-visible spectroscopy. The results show that Lys-CDs could coordinate with Zn 2+ and the adding of FA inhibits the coordination of Lys-CDs with central ions of Zn. The Lys-CDs and FA are successfully exposed with the NMOFs disintegrating in the acid environment of bacterial metabolites. We are surprised to find a sharp increase of reactive oxygen species (ROS) inside the bacterial cells by FA functionalizing NMOFs, which undoubtedly enhance the antibacterial and antibiofilm activity. The as-synthesized ZIF-8-based nanocomposites also show the peroxidase-like activity in an acid environment, and produce extremely active hydroxyl radicals resulting in the improved antibacterial and antibiofilm activity. The possible mechanisms of antibacterial activities indicate that the presence of FA is significant in the sense of targeting bacteria. This study shows a novel approach to construct acid stimulation supply system which may be helpful for the research of antibiofilms.
研究领域:
材料科学
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纳米技术
·
生物医学工程
·
微生物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2024
·
IF 9.4
Zhuo-Ran Yang,Huimin Qin,Jing-Wen Fan,Kehan Du,Liya Qi,Dandan Hou,Hao Jiang,Jintao Zhu
Drug-resistant biofilm infection is an extremely serious clinical problem, that easily leads to failure of antibiotic treatment. Although gold nanoparticles (AuNPs) as photothermal agents have been widely used in biofilm eradication, there are still challenges to be addressed, such as insignificantly redshifted absorption and slow assembly process of aggregated AuNPs. Herein, we developed an acidity-activated dispersion-to-aggregation transition to enhance the accumulation of self-complementary zwitterionic peptide-decorated AuNPs for photothermal eradication of drug-resistant biofilm infections. AuNPs were decorated with self-complementary zwitterionic peptides (ZP1 and ZP2) coupled with pH-sensitive anhydride (DMA) and pH-insensitive anhydride (SA), respectively. ZP2-decorated AuNPs with DMA modification (AuNP@ZP2(DMA)) exhibited prolonged blood circulation and enhanced accumulation in acidic biofilm microenvironment. Moreover, the electrostatic attraction between self-complementary ligands drove AuNPs to form closely packed aggregates with strong near-infrared absorption, leading to in vivo photoacoustic imaging ability and photothermal effect against drug-resistant bacteria and fungus, as well as microbial biofilms. AuNP@ZP2(DMA) with longer charge domains and a polyethylene glycol oligomer spacer showed greater photothermal antimicrobial and biofilm resistance in vitro and in vivo . This study develops an innovative acidity-activated AuNP photothermal agent, which provides an effective approach for treatment of biofilm infections.
研究领域:
纳米技术
·
生物医学工程
·
药学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOMATERIALS
·
2021
·
IF 12.479
Xianqi Zhu,Lin Li,Jin Tang,Chunyu Yang,Hao Yu,Kunpeng Liu,Ziyan Zheng,Xinggui Gu,Qingsong Yu,Fu-Jian Xu,Zhihua Gan
Metastasis has been widely recognized as the most lethal threats for cancer patients . Due to their special genetic and environmental context, cancer stem cells (CSCs) which are resistant to most cytotoxic drugs and radiation, are considered as the dominant culprit for metastasis. Thus, the efficient targeting and thorough elimination of CSCs are significantly urgent for the enhancement of therapeutic efficacy. Herein, we developed a facile and smart photothermal-chemo therapeutic nano-assembly system, of which the surface was modified by a sheddable PEG shell and acid-activatable pro-penetration peptide, to surmount the physiological barriers in targeting CSCs. A highly-efficient diradical-featured croconium-based photothermal agent and a natural cytotoxic heat shock protein (HSP) inhibitor were co-loaded in redox-sensitive chitosan matrices to realize the synergistic photothermal-chemo therapy. Within solid tumors, the PEG shell that prevents the nano-assembly from mononuclear phagocytic clearance could rapidly leave to expose the positively charged chitosan, and the detached iRGD could further actuate the tumor penetration of chitosan nanoparticles , and allow the CSCs targeting by selective recognition of CD44 protein. Owing to the HSP inhibition and chemo-sensitization, both the CSCs and non-CSCs could be thoroughly eliminated by the designed nano-assembly, largely inhibiting the tumor growth and metastasis. This work provides a potential strategy for CSCs-targeting drug delivery to solve the CSCs-related metastasis.
研究领域:
肿瘤学
·
纳米技术
·
药物递送系统
·
癌症生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）