Fermentation-Basel
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2023
·Vol. 9
·期 9
·818
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IF 3.7
Shuang Zhang,Pan Zhao,Ming Gao,Chuanfu Wu,Qunhui Wang,Xiaohong Sun
Anaerobic digestion (AD) has the advantages of utilizing complex substrates and producing renewable energy and is currently one of the mainstream technologies for food waste (FW) resourcing. However, at high organic loads and low inoculum-to-substrate ratios (ISRs), AD with FW as substrate is prone to acid accumulation, resulting in a drastic decrease in gas production and system collapse. This study investigated the effect of the coupled addition of zero-valent iron (ZVI) and activated carbon (AC) on the AD of FW at three low ISRs of 0.715, 0.625, and 0.5. The results showed that the control group acidified and stopped producing biogas when the ISR decreased to 0.625 and 0.5, but ZVI coupled with AC alleviated the acidification and increased the cumulative biogas yield. Especially at ISR = 0.5, the cumulative biogas yield for the ZVI + AC group was 31.5%, 99.5%, and 11.43 times higher than that of the ZVI, AC, and control groups, respectively. ZVI coupled with AC also increased the degradation of volatile fatty acids (70.5–84.4%) and soluble chemical oxygen demand (50.0–72.9%) while decreasing propionate concentration and improving the stability of the AD system. COD mass balance analyses indicated that the coupled addition of ZVI and AC promoted the conversion of particulate organic matter to soluble organic matter and increased the conversion of carbon sources to methane.Keywords:fermentation;methane production;acidification;acid accumulation
研究领域:
环境科学
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微生物学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF BIOTECHNOLOGY
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2025
·
IF 3.9
Chengtuo Niu,Huating Chen,Jinjing Wang,Chunfeng Liu,Qi Li
In beer production, lager yeasts are subjected to harsh environment in high-gravity brewing (HGB, 24°P or more), thus leading to reduced fermentation performance, increased mortality and formation of off-flavors. This study aimed to improve the vitality, viability and fermentation characteristics of lager yeast during HGB through the accumulation of intracellular proline and to reveal the potential mechanism. A mutant lager yeast Y-100 with significantly increased intracellular proline fluorescence intensity of 37.37 % was obtained by Atmospheric and Room Temperature Plasma (ARTP) mutagenesis. Compared to parental YY, the mutant Y-100 had 13.94 % higher intracellular ATP content, 23.01 % lower ROS accumulation and 77.71 % lower mortality rate at the end of serial batch fermentation for 5 times. Moreover, the time for beer matureness by Y-100 strain was shorted by one day while the real degree of fermentation value was 2.76 % higher using 24°P wort. Through genome resequencing, RT-qPCR analysis and gene knockout and overexpression, the up-regulation of GNP1 and SUA7 genes in Y-100 strain might contribute to the proline accumulation in lager yeast cells, thus resulting in energy supply and stress protection for lager yeast during HGB. The results not only provided new insights into the role of proline in lager yeast towards unfavorable industrial condition, but also obtained a high-efficient Y-100 strain for potential HGB application.
研究领域:
微生物学
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发酵工程
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食品科学
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产品关联
DOI
PubMed
高影响因子
ACS Sustainable Chemistry & Engineering
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2021
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IF 8.198
Shenyao Wang,Xiaoyu Lv,Zhenyu Zhao,Yixin Chen,Zhong Chen,Wenjun Lin,Haoran Li,Congmin Wang
A novel method of using anion-functionalized ionic liquids (ILs) with high NO absorption capacity and reversibility for the capture and in situ oxidation of low-concentration NO was established. For the mixed gas containing 0.25% NO, the accumulation rate of nitric acid in these ILs was significantly higher than that in the conventional ILs; after absorption and oxidation reached equilibrium, the molar ratio of HNO3 to [P4446][PhSO3] was 0.86, which was 5 times as high as that of the conventional IL. Therefore, the problems of low efficiency and poor reversibility of traditional ILs in the absorption and oxidation process were solved. Through absorption and oxidation experiments, quantum chemical calculation, NMR, and time-resolved in situ ATR-FTIR spectroscopic investigation, the results demonstrated that such a high accumulation rate of HNO3 originated from high uptake of NO through multiple-site interaction. Moreover, high reversibility is attributed to low volatility of conjugated acid of anions and decreased basicity of O sites on anions. We believe that such highly efficient and reversible conversion of low-concentration NO to HNO3 by ILs provides a new strategy for improving atomic economy of fossil fuel combustion, that is, converting post-combustion pollutants to good valuable chemicals.
研究领域:
环境科学
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化学工程
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材料科学
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DOI
高影响因子
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
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2013
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IF 3.548
Tingting Wu,Gefu Zhu,Ajay Kumar Jha,Ran Zou,Lin Liu,Xu Huang,Chaoxiang Liu
In the process of fermentative bio-hydrogen production, organic matter is degraded into volatile fatty acids and ethanol, and consequently thermodynamic constraints prevent the further spontaneous reactions. Moreover, the anaerobic baffled reactor (ABR) with the advantage of phase separation could realize the acetic acid accumulation via acid-producing bacteria. The microbial electrolysis cell (MEC) could convert the acetic acid into hydrogen furthermore. Considering this, the ABR coupled with MEC has been operated to strengthen the hydrogen production . This study was designed regarding orthogonal experiments to operate the four-compartment ABR with a hydraulic retention time (HRT) = 24 h, influent COD ∼ 4600 mg/L, temperature = 35 ± 1 °C, and discovered the optimal parameters with pH = 7, C/N = 44, and 2-bromoethanesulfonate (BES) concentration = 20 mmol/L to achieve the high acetic acid accumulation of effluent. The single-chamber membranless MEC with carbon anode and Ni-catalyst stainless steel cathode (liquid volume = 85 mL) fed with the ABR effluent with applied voltage = 0.6 V and electric conductivity = 7.45 mS/cm, achieved 99.0 ± 0.3% total COD removal efficiency, 1.31 ± 0.04 m 3 H 2 /m 3 d hydrogen production , 2.78 ± 0.11 mLH 2 /mgCOD hydrogen yield and 138.63 ± 3.11% electrical energy efficiency. Therefore the ABR-MEC system could overcome the “fermentation barrier” to achieve higher hydrogen production and organic matter degradation.
研究领域:
环境工程
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微生物学
✓ 已验证
DOI
高影响因子
Nature Communications
·
2025
·Vol. 16
·期 1
·1-19
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IF 15.7
Shen Xuecheng,Qiao Wenxuan,Yan Wei,Xie Hao,Zhang Chenyang,Sun Yang,Luo Qiong,Xu Qiang
IL-17 signaling contributes to the pathogenesis of psoriasis; however, IL-17 involvement in keratinocyte hyperactivation of epidermis remains unclear. Here, we describe an IL-17A-induced, skin-specific, positive feedback loop, which operates independently of canonical chemokine production, thus untangling skin inflammation and epithelial hyperproliferation in psoriasis. We show that IL-17A-induced, keratinocyte-specific KLK8 interacts with IL-17R to promote histone H4 lysine lactylation (H4K12la) catalyzed by the acetyltransferase HAT1. H4K12la further promotes IL-17A-mediated keratinocyte proliferation and the expression of KLK8 and IL-17R, creating a feedback loop that drives psoriasis progression. Importantly, excessive lactate in the microenvironment exacerbates H4K12la and psoriasis severity, thereby impairing the efficacy of anti-IL-17A antibody. Silencing KLK8, HAT1, or inhibiting lactate accumulation attenuates psoriasis in mice. Moreover, combining lactylation inhibition with anti-IL-17A therapy exhibits synergistic effects against antibody-resistant psoriasis. Thus, our findings unveil a lactylation-driven, keratinocyte-specific IL-17A signaling and offer a promising approach for psoriasis treatment, particularly in patients with comorbid metabolic syndrome.
研究领域:
皮肤生物学
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表观遗传学
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免疫学
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分子生物学
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炎症性疾病
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转化医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Plant Science
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2021
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IF 5.754
Zhu Yutao,Hu Xiaoqian,Wang Ping,Gao Linying,Pei Yakun,Ge Zhaoyue,Ge Xiaoyang,Li Fuguang,Hou Yuxia
Patatin-like proteins (PLPs) have non-specific lipid acyl hydrolysis (LAH) activity, which can hydrolyze membrane lipids into fatty acids and lysophospholipids. The vital role of PLPs in plant growth and abiotic stress has been well documented. However, the function of PLPs in plant defense responses against pathogens is still poorly understood. Here, we isolated and identified a novel cotton (Gossypium hirsutum) PLP gene GhPLP2. The expression of GhPLP2 was induced upon treatment with Verticillium dahliae, the signaling molecules jasmonic acid (JA) and ethylene (ETH) in cotton plants. Subcellular localization revealed that GhPLP2 was localized to the plasma membrane. GhPLP2-silenced cotton plants were more susceptible to infection by V. dahliae, while the overexpression of GhPLP2 in Arabidopsis enhanced its resistance to V. dahliae, which was apparent as mild symptoms, and a decrease in the disease index and fungal biomass. The hypersensitive response, deposition of callose, and H2O2 accumulation triggered by V. dahliae elicitor were reduced in GhPLP2-silenced cotton plants. The overexpression of GhPLP2 in Arabidopsis resulted in the accumulation of linoleic acid (LA, 18:2) and α-linolenic acid (ALA, 18:3) and facilitated the biosynthesis of JA and JA-mediated defensive responses. GhPLP2 silencing in cotton plants consistently reduced the accumulation of linoleic acid (LA, 18:2) and α-linolenic acid (ALA, 18:3) and suppressed the biosynthesis of JA and the defensive responses mediated by JA. These results indicate that GhPLP2 is involved in the resistance of cotton to V. dahliae by maintaining fatty acid metabolism pools for JA biosynthesis and activating the JA signaling pathway.
研究领域:
植物病理学
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分子生物学
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农学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENERGY
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2019
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IF 5.537
Shuaishuai Ma,Hongliang Wang,Jingxue Li,Yu Fu,Wanbin Zhu
Lignocellulosic biomass holds great potential in the production of renewable energy such as biomethane via anaerobic digestion . However, the digestion performance of the three main compositions of lignocellulose during biogas production is not well understood so far, and this has greatly hindered the efficient use of biomass by anaerobic fermentation. In this study, the anaerobic digestion performances of glucose, cellulose , hemicellulose , lignin, and their combinations in biomethane production were investigated. The biomethane production potential of cellulose was higher than that of hemicellulose and lignin, and high crystallinity cellulose shows adverse impact on methane production. Hemicellulose had a fast hydrolysis rate and was easier to be digested than cellulose, but it also easily led to excessive acids accumulation. Lignin showed a very limited potential in biomethane production. The co-fermentation of cellulose and hemicellulose could promote cellulose conversion and eliminate the over-acidification of hemicellulose, resulting in efficient and stable biogas production . The co-fermentation of lignin with cellulose and hemicellulose showed an inhibition in biomethane production, and lignin with different structure and sulfur content had different degree of inhibition. This study provides insights into the mechanisms of anaerobic fermentation of lignocellulosic biomass, and paves the way for the better use of biomass.
研究领域:
环境科学
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生物能源
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生物技术
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可再生能源
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）