BMC PLANT BIOLOGY
·
2023
·Vol. 23
·期 1
·1-14
·
IF 5.3
Li Guohui,Song Cheng,Manzoor Muhammad Aamir,Li Daoyuan,Cao Yunpeng,Cai Yongping
Background The enzyme phenylalanine ammonia lyase (PAL) controls the transition from primary to secondary metabolism by converting L-phenylalanine (L-Phe) to cinnamic acid. However, the function of PAL in pear plants ( Pyrus bretschneideri ) has not yet been fully elucidated. Results We identified three PAL genes ( PbPAL1 , PbPAL2 and PbPAL3 ) from the pear genome by exploring pear genome databases. The evolutionary tree revealed that three PbPAL s were classified into one group. We expressed PbPAL1 and PbPAL2 recombinant proteins, and the purified PbPAL1 and PbPAL2 proteins showed strict substrate specificity for L-Phe, no activity toward L-Tyr in vitro, and modest changes in kinetics and enzyme characteristics. Furthermore, overexpression of PbAL1 and PbPAL1 -RNAi, respectively, and resulted in significant changes in stone cell and lignin contents in pear fruits. The results of yeast one-hybrid (Y1H) assays that PbWLIM1 could bind to the conserved PAL box in the PbPAL promoter and regulate the transcription level of PbPAL2. Conclusions Our findings not only showed PbPAL’s potential role in lignin biosynthesis but also laid the foundation for future studies on the regulation of lignin synthesis and stone cell development in pear fruit utilizing molecular biology approaches.
研究领域:
植物学
·
分子生物学
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DOI
高影响因子
Science China-Chemistry
·
2017
·Vol. 60
·期 9
·1243-1249
·
IF 4.132
Zhang Honghua,Liu Ruixia,Zhang Ruirui,Huo Feng,Yang Zhiqiang,He Bin,Zhang Suojiang,Wang Yanji
Ionic liquid coupled with strong acid systems presents considerable promise in some catalytic fields. In the present work, the multiple complex systems composed by 98 wt% concentrated sulfuric acid and [Bmim][SbF 6 ] were investigated in the terms of stability, acidity and interaction properties. It was found that acidolysis of [Bmim][SbF 6 ] occurred in the 98 wt% concentrated sulfuric acid accompanied by HF releasing and SbF
− 6
degrading to [SbF 6− y
(HSO 4 )
y
] − . The species after acidolysis in the multiple complex systems were checked and confirmed by electrospray ionization mass spectrometry (ESI-MS), Fourier transform infrared spectroscopy (FT-IR), 1 H NMR and 19 F NMR. Acidity increased slightly with less than 1 wt% [Bmim][SbF 6 ] addition, while decreased with more proportion, which was determined based on the Hammett acidity functions H 0 , using 13 C NMR. The strong hydrogen bond S–O–H···F of interaction among the multiple complex systems was confirmed by molecular dynamic simulation.
研究领域:
化学
·
催化领域
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DOI
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2023
·
IF 9.8
Lucheng Li,Zehua Zhu,Jie Ni,Xiaojun Zuo
Phosphorus removal has been explored for a long time, however sustainable phosphorus adsorption and recovery with adsorbents recycling is rarely reported. This work proposes a sustainable phosphorus recycling route with calcium-modified powdered activated carbon with chitosan (Ca-PAC-CTS). The morphology, functional groups and crystal structure of Ca-PAC-CTS were characterized. The maximum phosphorus adsorption capacity was 16.73 mg/g Ca-PAC-CTS with Langmuir model at 298 K. Stable phosphorus sorption on Ca-PAC-CTS could be observed at the large range of pH (4– 10) when coexisting with NO 3 − , SO 4 2− , Cl − and F − , except HCO 3 − . 98.95 % The recovery of adsorbed phosphorus could get to 98.95 % using 0.05 M sulfuric acid solution, and the phosphate adsorption efficiency through Ca-PAC-CTS remained to be more than 80 % after five adsorption-desorption cycles, suggesting that Ca-PAC-CTS was one of the promising adsorbents for sustainable removal and recovery of phosphorus in aqueous solution.
研究领域:
环境科学
·
材料科学
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产品关联
DOI
PubMed
高影响因子
STARCH-STARKE
·
2022
·
IF 2.688
Junhui Li,Yuanhao Deng,Hanyi Feng,Hao Yuan,Yizhou Fang,Yunyun Yang,Guangrong Hunag
The modification of starch by a single freeze-thawing process and acid hydrolysis has been reported, while the combination of freeze-thawing and acid hydrolysis on the microstructure and physicochemical properties is unknown. The present study investigates the supporting effect of freeze-thawing pretreatment by comparing single modifications with the corresponding freeze-thawing assisted acid hydrolyzed starch in terms of granular, physicochemical, and thermal properties. Starch samples treated by combined treatment show the most severe damage on the surface and the oil adsorptive capacity is enhanced from 69.7% to 109.4%. In addition, the solubility of starch granules is notably improved by 35.9% and the swelling power decreases by 39.7% via the combined treatment, respectively. The results of Fourier transform infrared spectroscopy and X-ray diffraction indicate that in comparison with native corn starch, the chemical groups of modified starch processed by combined treatment are not altered, while the relative crystallinity significantly increases from 31.4% to 33.9%. Significant changes in the thermal effects are attributed to molecular rearrangements after hydrolysis of amylose in the amorphous regions. Therefore, the combined treatment would be an efficient and useful method to prepare functional starch.
研究领域:
食品科学
·
材料科学
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DOI
高影响因子
Frontiers in Plant Science
·
2017
·
IF 4.291
Zha Liangping,Liu Shuang,Liu Juan,Jiang Chao,Yu Shulin,Yuan Yuan,Yang Jian,Wang Yaolong,Huang Luqi
The content of active compounds differ in buds and flowers of Lonicera japonica (FLJ) and L. japonica var. chinensis (rFLJ). Chlorogenic acid (CGAs) were major active compounds of L. japonica and regarded as measurements for quality evaluation. However, little is known concerning the formation of active compounds at the molecular level. We quantified the major CGAs in FLJ and rFLJ, and found the concentrations of CGAs were higher in the buds of rFLJ than those of FLJ. Further analysis of CpG methylation of CGAs biosynthesis genes showed differences between FLJ and rFLJ in the 5′-UTR of phenylalanine ammonia-lyase 2 (PAL2). We identified 11 LjbZIP proteins and 24 rLjbZIP proteins with conserved basic leucine zipper domains, subcellular localization, and electrophoretic mobility shift assay showed that the transcription factor LjbZIP8 is a nuclear-localized protein that specifically binds to the G-box element of the LjPAL2 5′-UTR. Additionally, a transactivation assay and LjbZIP8 overexpression in transgenic tobacco indicated that LjbZIP8 could function as a repressor of transcription. Finally, treatment with 5-azacytidine decreased the transcription level of LjPAL2 and CGAs content in FLJ leaves. These results raise the possibility that DNA methylation might influence the recruitment of LjbZIP8, regulating PAL2 expression level and CGAs content in L. japonica.
研究领域:
植物学
·
分子生物学
·
药用植物研究
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产品关联
DOI
PubMed
高影响因子
Polymer Chemistry
·
2024
·Vol. 15
·期 47
·4824-4834
·
IF 4.1
Zidie Song,Li Liu,Pengbo Zhang,Kangle Xue,Zibo Hua,Tao You,Yiqi Wu,Hong Cui,Zhen Hu,Yudong Huang
Degradable epoxy resins are designed to address the environmental pollution caused by traditional epoxy resins. However, incorporating the degradability of dynamic covalent bonds in the design of degradable resins often compromises the rigidity and heat resistance of the material, rendering it less suitable for high-temperature applications, such as those used in deep space exploration. This paper presents the creation of an innovative, entirely bio-based epoxy monomer. Its cyclic rigid dynamic bonds confer a high tensile modulus (4.06 GPa), glass transition temperature (Tg = 240 °C), notable creep resistance with full shape recovery at 180 °C, and remarkable degradability, dissolving entirely in a 1 M HCl solution in just 60 minutes at 50 °C. The preparation of the diacetal was accomplished by reacting vanillin with xylitol under acidic conditions. This was followed by a reaction with bio-derived epichlorohydrin to produce bio-based, multifunctional epoxy monomers featuring hydroxyl groups and bicyclic acetal structures, representing a new strategy for eco-friendly polymer synthesis. This monomer was then cured with a hardener to create an epoxy crosslinked network. The eco-friendly raw materials are easily obtainable, and the synthesized cross-linked network structure is exceptionally high-performing. This makes it a potential material for use in deep space exploration and other tough environments, and it provides a benchmark for synthesizing chemically degradable epoxy resins suitable for extreme conditions.
研究领域:
材料科学
·
环境科学
·
化学工程
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DOI
高影响因子
Food Bioscience
·
2024
·
IF 4.8
Xiaoqiang Zou,Mudassar Hussain,Imad Khan,Yanxi Wang,Bangzhi Jiang,Lei Zheng,Yuechao Pan,Jijie Hu,Azqa Ashraf
Medium- and long-chain structured lipids (MLSLs) rich in docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) were synthesized by acidolysis of fish oil (Omax 1812, a mixture of sardine and anchovy oils) with caprylic acid (CA) using a lipase bio-imprinted with fatty acids as a catalyst. A bio-imprinted lipase for the acidolysis reaction was first prepared under optimal conditions, and its activity was 1.43 times higher than that of the non-bioimprinted. The bio-imprinted lipase was used as a catalyst to produce such MLSLs, and the reaction conditions were optimized. Under the optimal conditions, equilibrium was achieved in 8 h, and the total and the sn -1, 3 CA contents in the obtained product were 28.72% and 42.26%. Additionally, the total and the sn -2 DHA contents were 14.23% and 21.10%, respectively, and the total and the sn -2 EPA contents were 16.43% and 10.14%, respectively. These obtained MLSLs, rich in sn -1, 3 CA, and sn -2 DHA, EPA can deliver significant health benefits.
研究领域:
生物化学
·
食品科学
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产品关联
DOI
高影响因子
JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY
·
2013
·Vol. 91
·期 1
·55-62
·
IF 1.592
Qin Xiao-Li,Huang Hui-Hua,Lan Dong-Ming,Wang Yong-Hua,Yang Bo
Typoselectivity of crude CBD-T1 lipase ( Geobacillus sp. T1 lipase fused with a cellulose binding domain) was investigated. Multi-competitive reaction mixtures including a set of n -chain fatty acids (C8:0, C10:0, C12:0, C14:0, C18:1 n-9, C18:2 n-6 and C18:3 n-3) and tripalmitin-enriched triacylglycerols were studied in hexane. The crude CBD-T1 lipase discriminated strongly against C18:1 n-9 [competitive factor ( α ) = 0.23] and showed the highest preference for C8:0 ( α = 1). Utilizing the catalytic properties of crude CBD-T1 lipase, acidolysis of soybean oil with C8:0 was selected as a model reaction to investigate the ability of the lipase to produce MLM-type (medium-long-medium) structured lipids. Several reaction parameters (added water amount, reaction temperature, substrate molar ratio and reaction time) examined for incorporating C8:0 into soybean oil, the optimum conditions were: 1:3 (soybean oil/C8:0) of molar ratio, 3 mL of hexane, 50 °C of temperature, 48 h of reaction time, 20 % of crude CBD-T1 lipase (w/w total substrates), and 7.5 % of water (w/w enzyme). Under these conditions, the incorporation of C8:0 was 29.6 mol%. The results suggest that crude CBD-T1 lipase, which showed different fatty acid specificity profiles, is a potential biocatalyst for the modification of fats and oils.
研究领域:
生物化学
·
食品科学
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DOI
高影响因子
Journal of Cleaner Production
·
2024
·
IF 9.7
Mingyuan Liu,Yuchen Meng,Xinyang He,Jiatai Gu,Zhen Li,Shu Yang,Yue Zhang,Maorong Zheng,Liming Wang,Xiaohong Qin
The utilization of starch to treat sewage that received widespread attention has become one of the promising ways to purify water due to the advantages of low cost and biodegradability. However, the poor purification capacity for wastewater and difficult liquid phase dispersion of natural starch severely hindered its application in wastewater treatment and limited the development of starch materials. Hence, a series of cationic starches with different particle sizes were fabricated by controlling the acidification time and combining starch with N-2,3-epoxy-propyl trimethyl ammonium chloride to improve the dispersion and purification effect of starch in water. Acid hydrolysis could destroy the spatial structure of starch, which improved the etherification property to enhance the purification property of starch to different pollutants and the dispersed stable state in water. The maximum adsorption capacities of the optimal functional agents were 43.2 mg/g (methyl orange), and 236.8 mg/g (acid black) and the optimal penetration rate of the treated kaolin solution was 94.6%. The working behavior of cationic starch could be composed of rapid, stable, and equilibrium stages, and the working process of HS-g-GTA conformed to the pseudo-second-order dynamic model. This work provides a simple and low-energy pretreatment method for preparing cationic starch, which has important application value in removing anionic substances from wastewater.
研究领域:
环境科学
·
材料科学
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产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Feng Li,Jianwan Lin,Yongqi Li,Shijia Wu,Cha Wen,Mengting Mo,Linfeng Huang,Ning Qing,Zhenpin Lu,Xing Jiang,Liuyan Tang
While hydrogel coatings have garnered considerable attention in recent years, the utilization of bioresources has been largely overlooked in existing preparation strategies. This study presents a hydrogel coating fabrication method utilizing shell waste-derived shell powder (SP) as a key resource, termed shell powder acidolysis triggered in situ gelation (SP-ATIG). By integrating a SP containing tannic acid-polyvinyl alcohol primer (PVA-TA/SP layer) with sodium alginate, the SP-ATIG method enabled the formation of hydrogel coatings on both flat and irregular surfaces of various substrates (e.g., glass, plastics, metals) with controllable thickness. This method is also capable of constructing dual-network hydrogel coatings, providing substrates with tailored functional surfaces. Moreover, it endowed the resulting hydrogel coatings with patterning capabilities, lubricity, and anti-oil properties, offering them potential applications such as information transmission, self-lubricating materials, and anti-fouling coatings. This work highlights the innovative use of waste bioresources to develop versatile hydrogel coatings, providing a sustainable alternative for advanced functional surface fabrication.
研究领域:
材料科学
·
环境科学
·
表面工程
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产品关联
DOI
高影响因子
POLYMER
·
2024
·
IF 4.1
Huiting Yu,Xi Sun,Zheng Liu,Heguo Han,Jianhang Qiu,Qifeng Zhang,Yuxuan Sun,Shenghai Li,Suobo Zhang
Semi-crystalline poly (ether ketone) (PEK) materials with intrinsic solvent resistance are ideal materials for the preparation of organic solvent nanofiltration (OSN) membranes. Although some precursor materials have been developed for soluble processing through group protection strategies, these precursors are difficult to hydrolyze, limiting their practical applications. In this work, we designed an easily hydrolyzable N-cyclohexyl-poly (ether ketone ether imide) (CYH-PEKEI) precursor that could be converted to PEK by acid treatment at 80°C within 1 h. The results showed that the prepared membrane was stable in various organic solvents, with a permeance of 1.29 L m −2 h −1 bar −1 in N, N-Dimethylformamide (DMF), a molecular weight cutoff of 320 g/mol, and good long-term operational stability. Additionally, the membrane demonstrated excellent aging resistance during variable temperature DMF operation. This work has stimulated the potential of PEK membranes for OSN applications by optimizing the precursor structure.
研究领域:
材料科学
·
化学工程
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产品关联
DOI
高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2022
·Vol. 102
·期 11
·4714-4723
·
IF 4.125
Ke Cheng,Jianghua Zhang,Shasha Yang,Fawen Yin,Zixuan Li,Ting Wang,Dayong Zhou
BACKGROUND Efficient and sustainable production of structured phospholipids (SPLs) enriched in medium-chain fatty acids (MCFAs) in a heterogeneous manner is crucial for their potential applications in functional foods and drugs. Herein, for the first time, Zn- and Al-incorporated SBA-15 silicas were prepared by the coprecipitation method and further researched for catalytic synthesis of MCFA-enriched SPLs through acidolysis reaction of natural phospholipids with capric or caprylic acid. RESULTS The as-prepared Zn- and Al-incorporated SBA-15 samples exhibited superior catalytic activities under mild experimental conditions (50 °C, 6 h) to commercial homogeneous Lewis acids and benchmark enzymes. Correspondingly, the capric acid and caprylic acid incorporations were respectively achieved up to ~40.25 ± 0.40% (or 35.08 ± 0.09%) and 37.26 ± 0.38% (or 33.02 ± 0.13%) for Zn- (or Al-) incorporated SBA-15 catalyst. Moreover, various methods such as scanning electron microscopy with energy-dispersive X-ray spectrometry, ultraviolet–visible diffuse reflectance spectroscopy and pyridine–Fourier transform infrared spectroscopy were utilized to characterize the two catalysts in order to elucidate the possible structure–performance relationship. Accordingly, the above-mentioned satisfactory results are most probably due to the well-ordered mesostructures and large amounts of active Lewis acid sites existing in the investigated materials. Noticeably, the two catalysts featured good separation and excellent recyclability as well. CONCLUSION The Zn- and Al-incorporated SBA-15 catalysts studied in this work might shed light on novel, sustainable and economic alternatives for effective SPL production to diminish the applications of conventional homogeneous catalysts and biocatalysts in food industries. © 2022 Society of Chemical Industry.
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
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