BIOMACROMOLECULES
·
2012
·
IF 5.479
Xiaojun Cai,Chunyan Dong,Haiqing Dong,Gangmin Wang,Giovanni M. Pauletti,Xiaojing Pan,Huiyun Wen,Isaac Mehl,Yongyong Li,Donglu Shi
A dual stimulus-responsive mPEG-SS-PLL15-glutaraldehyde star (mPEG-SS-PLL15-star) catiomer is developed and biologically evaluated. The catiomer system combines redox-sensitive removal of an external PEG shell with acid-induced escape from the endosomal compartment. The design rationale for PEG shell removal is to augment intracellular uptake of mPEG-SS-PLL15-star/DNA complexes in the presence of tumor-relevant glutathione (GSH) concentration, while the acid-induced dissociation is to accelerate the release of genetic payload following successful internalization into targeted cells. Size alterations of complexes in the presence of 10 mM GSH suggest stimulus-induced shedding of external PEG layers under redox conditions that intracellularly present in the tumor microenvironment. Dynamic laser light scattering experiments under endosomal pH conditions show rapid destabilization of mPEG-SS-PLL15-star/DNA complexes that is followed by facilitating efficient release of encapsulated DNA, as demonstrated by agarose gel electrophoresis. Biological efficacy assessment using pEGFP-C1 plasmid DNA encoding green fluorescence protein and pGL-3 plasmid DNA encoding luciferase as reporter genes indicate comparable transfection efficiency of 293T cells of the catiomer with a conventional polyethyleneimine (bPEI-25k)-based gene delivery system. These experimental results show that mPEG-SS-PLL15-star represents a promising design for future nonviral gene delivery applications with high DNA binding ability, low cytotoxicity, and high transfection efficiency.
研究领域:
生物医学工程
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分子生物学
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药剂学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF MEMBRANE SCIENCE
·
2023
·
IF 9.5
Bei-Na Dong,Liu-Yang Gong,Li Xia,Nian Zhang,Li-Fen Liu
In this study, a new kind of wholly-Tröger's base-based polyamide ( w TB-PA) thin film composite (TFC) nanofiltration (NF) membrane was successfully fabricated for the separation application in strong acidic condition via the acid-induced segregation (AIS) effect. Firstly, the self-synthesized Tröger's base diamine monomer — 2,8-diamino-4,10-dimethyl-6H,12H-5,11-methanodibenzo [1,5]-diazocine (TBDA) was added in the polyethersulfone (PES) casting solution to prepare the TBDA-based PES substrate by synchronously coupling the non-solvent-induced phase transformation (NIPs) and AIS, and then the TBDA molecules on the PES substrate surface further reacted with trimesoyl chloride (TMC) via interfacial polymerization (IP) to obtain the w TB-PA TFC NF membranes. Meanwhile, the acid-induced segregation effect of on the microstructure and separation performance of the w TB-PA TFC NF membrane were systematically investigated. As compared with the conventional poly(piperazine amide) NF membrane (M PIP-TMC ), the w TB-PA NF membranes exhibited thinner, smoother but more hydrophobic surface as well as lower isoelectric point, and especially showed much higher tolerance to 20 wt% H 2 SO 4 after soaking 63 days and favorable dye rejection (～99.9 %). Thereinto, the acid segregation agent of citric acid monohydrate (CA) endowed the w TB-PA TFC NF membrane with relatively optimal acid resistance and SO 4 2− /Cl − separation selectivity (about 11.8) than glacial acetic acid (AC), nitric acid (HNO 3 ) and hydrochloric acid (HCl). Moreover, this w TB-PA NF membrane based on CA-induced segregation had higher recovery of the cationic dye of crystal violet (CV) (＞98.0 %) in acidic condition. Therefore, the w TB-PA TFC NF membranes have great potential for the separation application in the strong acidic condition in the future.
研究领域:
材料科学
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化学工程
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环境科学
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产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Yang Hu,Tianyou Cui,Zhihong Yang,Lu Ren,Li Luo,Mengqian Zhao,Mingjie Jin,Xuwei Long
Sophorolipids (SLs) show great potential application in many fields as an alternative to chemically synthesized surfactants. However, their poor water solubility limits industrial applications in many fields. In this study, a new environmentally friendly strategy based on acid-induced chemical modifications has been developed to prepare and isolate water-soluble SLs from natural SLs that not dissolve or precipitate in fermentation broth. Over 70 % of SLs were obtained as sediments from fermentation broth after chemical modification at a pH of 1 at 120 °C for 5 min. The obtained SLs demonstrated excellent pH-regulated water solubility, superior foaming ability, and comparable surface activities with natural SLs. The involved mechanism of the present process was attributed to the generation of glucolipids with excellent water solubility. The present findings are conducive to preparing water-soluble SLs, addressing the problem that SLs cannot be recovered from fermentation broths via acid-induced precipitation in certain case.
研究领域:
生物化学
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环境科学
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工业应用
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产品关联
DOI
高影响因子
CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
·
2019
·Vol. 35
·期 4
·549-555
·
IF 1.071
Lu Xue,Liu Guocheng,Wang Xiang,Lin Hongyan,Wang Xiuli
We synthesized three new Ni(II) coordination polymers [Ni(L)(HIP)(H 2 O) 2 ]H 2 O(CP 1 ), [Ni(L)(NIP)]·2H 2 O(CP 2 ) and [Ni(L)(NDC)(H 2 O) 2 ](CP 3 )[L= N , N' -bis(pyridine-3-yl)thiophene-2,5-dicarboxamide, H 2 HIP=5-hydroxyisophthalic acid, H 2 NIP=5-nitroisophthalic acid, H 2 NDC=2,6-naphthalenedicarboxylic acid] by hydrothermal method, which were characterized by means of infrared spectra(IR), TG analyses, PXRD and single-crystal X-ray diffraction. The CP 1 is a 1D tubular structure based on [Ni-HIP] 2 loops and pairs of L ligands. CP 2 is a 2D 3,5-connected architecture, which consists of Ni-L linear chains and (Ni-NIP) 2 double chains. CP 3 is a 2D network, which features 4-connected topology. Solid-state luminescent behaviours of CP 1 —CP 3 were investigated. The CP 1 can detect Fe 3+ ions through luminescence quenching. The electrochemical properties of CP 1 buk-modified carbon paste electrode(CP 1 -CPE) has also been investigated, which has bifunctional electrocatalytic activity for oxidation of ascorbic acid and reduction of NO 2 − .
研究领域:
材料化学
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配位化学
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分析化学
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DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2021
·
IF 6.953
Xinxiang Li,Mengfan Yang,Wan Zhang,Kunlin Chen,Chaoxia Wang
A durable and reversible acid-induced discoloration azobenzene UV-curable lignin-based waterborne polyurethane polymeric dye (EDA-ULPD) is prepared from lignin, azobenzene and pentaerythritol triacrylate(PETA) by chemical modification of waterborne polyurethane. Lignin and PETA are chemically bonded to the polyurethane chain to improve thermal stability , UV resistance and color fastness, while also endow the polymeric dye with UV curing performance, which is a green and environmentally friendly fixing way. The acid-induced discoloration property of EDA-ULPD with azobenzene chromophore side chain is comparable to those of 4-ethyl-4-2,2′-dihydroxy diethylamine azobenzene (EDA). As the pH value decreases from 7 to 1, the maximum absorption peak of EDA-ULPD from 420 nm to 530 nm, and the color change from yellow to pink due to the transformation of EDA molecular structure from diazo to hydrazone. Interestingly, when EDA-ULPD is fixed to the fabric in the way of UV curing, its printed fabric exhibits the performance of high concentration acid-induced discoloration (1 mol·L −1 HCl) due to the cross-linked structure formed by EDA-ULPD. The acid-induced discoloration property of EDA-ULPD printed fabrics also presents outstanding repetitious stability. The stimulus response printed fabric with reversible high concentration acid discoloration possesses a broad application prospect in smart textiles.
研究领域:
材料科学
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化学工程
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产品关联
DOI
PubMed
高影响因子
Small
·
2024
·
IF 13
Songzhu Xing,Xujiao Ma,Qingqing Gu,Nana Ma,Zhong Zhang,Guoying Han,Rui Huang,Xiao Feng,Bing Yang,Chunying Duan,Yiwei Liu
The construction of defective sites is one of the effective strategies to create high-activity Metal–Organic frameworks (MOFs) catalysts. However, traditional synthesis methods usually suffer from cumbersome synthesis steps and disordered defect structures. Herein, a cluster–cluster co-nucleation (CCCN) strategy is presented that involves the in situ introduction of size-matched functional polyoxometalates (H 6 P 2 W 18 O 62 , {P 2 W 18 }) to intervene the nucleation process of cluster-based MOFs (UiO-66), achieving one-step inducement of exposed defective sites without redundant post-processing. POM-induced UiO-66 ({P 2 W 18 }-0.1@UiO-66) exhibits a classical reo topology for well-defined cluster defects. Moreover, the defective sites and the interaction between POM and skeletal cluster nodes are directly observed by Integrated Differential Phase Contrast in Scanning Transmission Electron Microscopy (iDPC-STEM). Owing to the molecular-level proximity between defective sites and POM in the same nano-reaction space, {P 2 W 18 }-0.1@UiO-66 exhibits efficient tandem catalysis in the preparation of γ-valerolactone (γ-GVL) from laevulinic acid (LA) by the combination of Lewis and Brønsted acids with 11 times higher performance than defective UiO-66 formed by conventional coordination modulation strategy. The CCCN strategy is applicable to different POM and has the potential to be extended to other cluster-based MOFs, which will pave a new way for the construction of functional MOFs with multi-centered synergistic catalysis.
研究领域:
材料科学
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催化化学
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DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2017
·
IF 6.216
Wenquan Huang,Jing Chong,Yong Tian,Xiufang Wang
To date, the pursuit of dual-meospores while maintaining definite dispersity and small particle size of mesoporous carbon nanospheres (MCN) still remains a great challenge, due to the aggregation tendency of small particles (especially below 200 nm) and the destruction of nanospherical structure after excessively creating mesopores . Herein, we report a high acid induced heterogeneous nucleation and growth approach to synthesize dual-mesoporous carbon nanospheres (DMCN) with well-controlled small diameters. This highly acidic driven method has effectively balanced the organic-organic, organic-inorganic and inorganic-inorganic assembly to control the polymerization kinetics, thus forming dispersed nanospheres. The surface incompatibility issues between silica nanoparticle (SN, the inorganics) and resorcinol/formaldehyde (RF, the organisms) were addressed through the employment of inorganic/ionic/organic interaction to synthesize DMCN under highly acidic condition. An extended I + X − S + mechanism is proposed to interpret this formation process. By precisely controlling the processes of growth and cooperation-assembly, the diameter can be easily manipulated over a wide range (130–60 nm), which is a facile route for preparing dispersed small-size DMCN. Meanwhile, a linear function model is established to quantitatively control and predict the particle size. Since the DMCN integrated both the advantages of small diameter (nanosize effect) and bimodal mesopore system (reduction of transport limitation) in one single unit, it exhibits an excellent performance in the release of poorly water-soluble drug and the adsorption of dye molecules.
研究领域:
材料科学
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纳米技术
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化学工程
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DOI
高影响因子
JOURNAL OF MOLECULAR STRUCTURE
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2021
·
IF 3.196
Guangxin Zhang,Jingmiao Hu,Haoran Xu
A defect state g-C 3 N 4 was synthesized from urea by thermal condensation and oxalic acid treatment. Various analytical methods such as X-ray diffraction, scanning electron microscopy, X-ray photoelectron spectroscopy were adopted to characterize the structures and properties of g-C 3 N 4 . The results showed that oxygen atoms replaced the carbon atoms of g-C 3 N 4 , which changed the band structure of g-C 3 N 4 . The catalytic performances of defect state g-C 3 N 4 were improved and exhibited better photocatalytic activity than pure g-C 3 N 4 . For the effects of acetic acid concentration, g-C 3 N 4 treated by 0.05 mol/L oxalic acid exhibited enhanced photocatalytic performance, which could be attributed to the suitable defect concentration. The photocatalytic degradation mechanism was proposed. Superoxide radicals instead of hydroxyl radicals played an important role in the rhodamine B degradation.
研究领域:
材料科学
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环境科学
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产品关联
DOI
高影响因子
ADVANCED ENGINEERING MATERIALS
·
2018
·Vol. 20
·期 7
·1701151
·
IF 2.576
Yanan Xue,Lijun Tao,Yang Zhou,Jie Liu,Bo Yu,Sihui Long,Shiwen Huang,Faquan Yu
To elevate the efficacy and to attenuate the adverse effects remain a tough challenge in exploring anticancer drug delivery systems. A nanocarrier characteristic of acid induced charge reversal and acid/redox/magnetic combinational stimuli-responsiveness is designed. This system exhibits weaker repulsion between the carrier and the cancer cell membrane, resulting in enhanced uptake. After nanocarriers enter the cell, the system exhibits negative-to-positive charge reversal in response to the low intracellular pH value. Moreover, after the uptake, the carrier will be further dissociated by glutathione in cytoplasm via the reduction of the disulfide bond, leading to the rapid release of the encapsulated DOX into the nuclei. In vitro release study expresses the sequent stimulus release profile. The antitumor activity tested in CT26 tumor-bearing mice reveals efficient therapeutic efficacy and as low adverse effect as PBS. H&E staining and immunohistochemical analysis of tumor tissues as well as pathological test of tissue sections of vital organs confirm the high antitumor activity and low tissue toxicity. Thus, the belief that this system has a good prospect in the field of cancer clinical chemotherapy.
研究领域:
药学
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生物医学工程
·
肿瘤学
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产品关联
DOI
高影响因子
ACS Sustainable Chemistry & Engineering
·
2016
·
IF 5.267
Fan Xu,Yiqing Chen,Minghui Tang,Haiyan Wang,Jiang Deng,Yong Wang
Hydrothermal carbonization of biomass is an economic and sustainable technique for the fabrication of functional carbonaceous materials. However, the introduction of biomass into synthesis of ordered mesoporous carbon materials achieves slow progress due to complicated requirements. It is still challenging to prepare biomass-derived ordered mesoporous carbon materials. Herein, we report a simple and effective strategy to synthesize ordered mesoporous carbon materials from renewable and environment-friendly pentose using one-pot hydrothermal synthesis. The as-synthesized ordered mesoporous carbon materials show mesopores of two-dimensional hexagonal symmetry and rich functional groups. Mechanism studies indicate that the acid facilitates the self-assembly of carbon precursors and soft templates and therefore induced the formation of ordered mesoporous carbonaceous materials under hydrothermal conditions. This is the first report on the S0H+X–I+ self-assembly mechanism that achieves success in biomass system. In addition, the unique materials show outstanding performance as supports in catalytic hydrogenation. Significantly, this acid induced self-assembly strategy will open up new avenues for designing functional biomass-derived carbon materials.
研究领域:
材料科学
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化学工程
·
可再生资源利用
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产品关联
DOI
高影响因子
Aquaculture Reports
·
2024
·
IF 3.2
Yuru Zhang,Haiying Fan,Kedi Gao,Shibo Zhang,Ronghua Lu,Xianglin Cao,Xinxin Xu,Jiancheng Liu
The grass carp ( Ctenopharyngodon idella ) holds significant economic value worldwide. However, intensive farming practices often result in lipid metabolic disorder of fish. It is essential to explore the influence of pivotal genes in the process of fat accumulation, particularly from the perspective of fat transport. Fatty acid binding proteins (FABPs) are crucial for selectively binding and transporting free fatty acids (FAs) within cells. Nevertheless, the types of fabp genes in the grass carp genome and their responses to various FAs remain unclear. In the current study, exhaustive bioinformatic analysis identified nine fabp genes in the grass carp genome: fabp1a, fabp1b.1, fabp2, fabp3, fabp6, fabp7a, fabp10a, fabp11a, and fabp11b . Although these genes showed widespread expression across various tissues, each gene displayed distinct preferences for specific primary expression tissues. Notably, fabp1a exhibited prominent expression in muscle, fabp1b.1 in abdominal fat, fabp2 and fabp6 in the intestine, fabp7a in the brain, fabp10a in the hepatopancreas, and fabp11a showed maximal expression in the eye. Findings from primary grass carp hepatocytes revealed that the addition of palmitic acid (PA), high concentrations oleic acid (OA) and docosahexaenoic acid (DHA) (400 µM), significantly increased intracellular lipid accumulation. Intriguingly, the addition of PA resulted in increased expression of fabp2 and fabp11a , while DHA significantly enhanced the expression of fabp7a ( P < 0.01). Given that the accumulated FAs are primarily saturated fatty acids, these results suggest that regulating fabps , especially fabp2 and fabp11a, could be a strategy to control fat deposition in grass carp.
研究领域:
水产养殖学
·
分子生物学
✓ 已验证
DOI
高影响因子
ANALYTICAL LETTERS
·
2017
·
IF 1.15
Lingzhi Zhao,Zhao Li,Liu Zhao,Chenxiao Zhang
The increasing number of patients suffering from tinnitus may be explained by an imbalance in the antioxidant defense system and reactive oxygen species formation, suggesting the importance of the redox homeostasis in this condition. Endogenous biothiols play important roles in maintaining redox homeostasis. Hence, to understand the role of biothiols in the pathological process of tinnitus, this study demonstrates an in vivo method for monitoring the concentrations of biothiols in the paraflocculus of the rat cerebellum during tinnitus induced by the injection of salicylate. Resorufin-based fluorescent probe 1 was used as the selective probe with in vivo microdialysis. Probe 1 was premixed with microdialysates from the paraflocculus of the rat cerebellum and transferred into a cuvette for continuous-flow fluorescence. A linear relationship between the fluorescence and the concentrations of cysteine, homocysteine, and glutathione was from 1 to 15 µM, with detection limits of 128, 148, and 183 nM, respectively. The basal level of total reduced biothiols in the paraflocculus of the rat cerebellum microdialysate was determined to be 10.62 ± 1.34 µM based on the calibration curve for homocysteine. The injection of sodium salicylate into the animals significantly decreased the reduced biothiol concentrations in the paraflocculus of the rat cerebellum from 60 min, reaching 39.5 ± 6.20% of the basal level. In contrast, at 120, 180, and 300 min, the concentrations of reduced biothiols were 55.73 ± 9.35, 58.26 ± 9.56, and 91.69 ± 6.91% of the basal level, respectively. These results imply that the decrease in reduced biothiols in the paraflocculus of the rat cerebellum may be associated with salicylate-induced tinnitus. This study offers a new platform for in vivo monitoring of reduced biothiols in the paraflocculus of the rat cerebellum following salicylate-induced tinnitus and may be useful for the investigation of this condition.
研究领域:
神经科学
·
药理学
·
分析化学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）