BIORESOURCE TECHNOLOGY
·
2011
·
IF 4.365
Xian-wei Sui,Zhi Wang,Bing Liao,Ying Zhang,Qing-xiang Guo
Fast pyrolysis of bagasse pretreated by sulfuric acid was conducted in a fixed bed reactor to prepare levoglucosenone (LGO), a very important anhydrosugar for organic synthesis. The liquid yield and LGO yield were studied at temperatures from 240 to 350 °C and sulfuric acid loadings from 0.92 to 7.10 wt.%. An optimal LGO yield of 7.58 wt.% was obtained at 270 °C with a sulfuric acid pretreatment concentration of 0.05 M (corresponding to 4.28 wt.% sulfuric acid loading). For comparison, microcrystalline cellulose pretreated by 0.05 M sulfuric acid solution was pyrolyzed at temperature from 270 °C to 320 °C, and bagasse loaded with 3–5 wt.% phosphoric acid was pyrolyzed at temperature from 270 °C to 350 °C. The highest yield of LGO from bagasse was 30% higher than that from microcrystalline cellulose, and treatment with sulfuric acid allowed a 21% higher yield than treatment with phosphoric acid.
研究领域:
生物化学工程
·
可再生资源利用
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
·
2015
·
IF 4.494
Hongsen Zhang,Jian Zhang,Jie Bao
This study reported a high titer gluconic acid fermentation using dry dilute acid pretreated corn stover (DDAP) hydrolysate without detoxification. The selected fermenting strain Aspergillus niger SIIM M276 was capable of inhibitor degradation thus no detoxification on pretreated corn stover was required. Parameters of gluconic acid fermentation in corn stover hydrolysate were optimized in flasks and in fermentors to achieve 76.67 g/L gluconic acid with overall yield of 94.91%. The sodium gluconate obtained from corn stover was used as additive for extending setting time of cement mortar and similar function was obtained with starch based sodium gluconate. This study provided the first high titer gluconic acid production from lignocellulosic feedstock with potential of industrial applications.
研究领域:
生物技术
·
发酵工程
✓ 已验证
DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
·
2023
·
IF 11.4
Hanqi Gu,Xushen Han,Jian Zhang,Jie Bao
Dry acid pretreatment (DAP) as a promising process for industrial biorefinery provide an efficient bioconversion of cellulose without free wastewater, although the partial xylan and lignin degrade to inhibitors or recondense. A biorefinery strategy for carbon efficient conversion of lignocellulose into bioethanol, xylose , and reactive lignin was developed by upgrading DAP with post-hydrolysis. The results showed that lignocellulose after mild DAP (175 °C, acid dosage of 15 mg/g dry material) obtained higher xylan recovery and lower inhibitors than that of general DAP. Subsequently, post-hydrolysis, simultaneous saccharification and ethanol fermentation were performed at solids loading of 20 wt% without detoxification and sterilization, resulting in xylose and ethanol yield of 71.8 % and 67.6 %. The fractionated lignin presented more reactive β-aryl ether linkages and less condensation than that from DAP. 66 % of lignocellulose carbon was recovered as ethanol, xylose and reactive lignin. This upgrading biorefinery strategy provided an easy-to-operate process for integrated utilization of lignocellulose.
研究领域:
生物技术
·
可再生能源
✓ 已验证
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2019
·
IF 8.355
Jialin Liang,Siwei Zhang,Maoyou Ye,Jinjia Huang,Xian Yang,Shoupeng Li,Shaosong Huang,Shuiyu Sun
Zero-valent iron (ZVI) combined with oxidants is a promising conditioning strategy for improving sewage sludge dewaterability. However, challenges, such as a low dewatering rate and utilization efficiencies of ZVI, restrict further application of ZVI/oxidant treatments. This study employs, for the first time, rapid and cost-effective in-situ generation of Fe 2+ combined with common oxidants, namely H 2 O 2 , Na 2 S 2 O 8 , and KMnO 4 , to effectively improve sludge dewaterability. A comparative investigation of a standalone ZVI/oxidants treatment and when combined with a thermal and acid-wash (TA) pretreatment (TA/ZVI/oxidants) was systematically conducted to determine the sludge dewatering efficiency. Under the optimal operational conditions, the average water content of dewatered cake (Wc) was 18.47% less with the TA/ZVI/oxidants treatment when compared with the ZVI/oxidants treatment. The Wc values decreased to 54.81, 58.06, and 59.32 wt%, after the TA/ZVI/H 2 O 2 , TA/ZVI/Na 2 S 2 O 8 , and TA/ZVI/KMnO 4 treatments, respectively. The potential mechanism by which the TA/ZVI/oxidants treatment enhanced dewatering was investigated by determining the characteristics of both the sludge and the ZVI surface structure. Results indicate that the TA/ZVI/oxidants process caused more Fe 2+ dissolution, more strong oxidizing radicals and more strong flocculating Fe 3+ . The large amount of strong oxidizing radicals and strong flocculating Fe 3+ caused cell lysis and disrupted the protein-like and polysaccharide-like materials in the EPS, thereby decreasing the particle size, negative zeta potential, viscosity and colloidal forces, which then released the bound water trapped within the net-like floc and led to improved sludge dewaterability. In the ZVI reuse experiment, the TA/ZVI/oxidants treatment remained highly effective for sludge dewatering even after four cycles. Furthermore, economic analysis indicates that the TA/ZVI/H 2 O 2 treatment can save approximately 52.32% per year compared with using the traditional polyacrylamide (PAM) treatment.
研究领域:
环境工程
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污水处理
✓ 已验证
DOI
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2024
·
IF 5.6
Ni Wei,Chundong Yang,Yan Su,Chenhuan Lai,Qiang Yong
To investigate the disparate effects of carbocation scavengers on promoting the enzymatic hydrolysis of softwood and hardwood, five carbocation scavengers were supplemented during acid pretreatment of masson pine and poplar in this study, including 2-naphthol (2 N), 2-naphthol-7-sulfonate (NS), p -hydroxybenzoic acid (PB), vanillic acid (VA) and syringic acid (SA). The results showed that naphthols (2 N and NS) with only one nucleophilic site enhanced delignification and enzymatic hydrolysis more significantly than the monophenols with several reactive sites. Adding 2 N and NS increased the hydrolysis yield of acid-pretreated masson pine (AM) from 14.87% to 20.58% and 35.52%, respectively. Correspondingly, the hydrolysis yield of acid-pretreated poplar (AP) improved from 56.54% to 80.47% and 86.9%, respectively. Moreover, as pretreatment severity increased, the enzymatic digestibility of AM and AP first increased and then decreased. However, the naphthols modification retarded this decline, especially for the enzymatic hydrolysis of pretreated poplar. The structure of carbocation scavengers and the lignin unit composition jointly determined the improvement on enzymatic hydrolysis of woody biomass by carbocation scavengers. Furthermore, the naphthols promoted the enzymatic hydrolysis mainly by alleviating enzyme non-productive binding.
研究领域:
生物化学
·
生物质能
✓ 已验证
产品关联
DOI
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2024
·
IF 5.6
Miaorou Dai,Yangyang Dong,Sen Ma,Lihui Gan,Lu Lin,Jian Liu
This work proposed a two-step pretreatment method of cooking with active oxygen and solid alkali (CAOSA) followed by dilute sulfuric acid treatment (DA), to contribute enzymatic hydrolysis of corn cob . The first step, CAOSA pretreatment , removed 83.16 % of the lignin, while the second step, a milder pretreatment with DA further eliminated hemicellulose . After the two-step pretreatment , the cellulose content of the residue reached 66.74 %, and with a solid loading of 5 %, the glucose yield was remarkably high at 98.35 % with a concentration of 36.47 g/L. The optimal conditions of the process were determined as follows: DA with 1 % H 2 SO 4 at a solid-to-liquid ratio of 1:10, a temperature of 110 ℃, and a pretreatment time of 60 min. To confirm the efficacy of the two-step pretreatment, we characterized the samples treated with different methods using X-ray diffraction (XRD), Fourier-transform infrared (FT-IR) spectroscopy, scanning electron microscopy (SEM), and X-ray photoelectron spectroscopy (XPS). The developed method proposes an effective pretreatment and results in the complete enzymatic hydrolysis of corn cob.
研究领域:
生物化学
·
可再生能源
✓ 已验证
DOI
高影响因子
APPLIED CATALYSIS A-GENERAL
·
2020
·
IF 5.006
Mingsheng Luo,Shuo Li,Zuoxing Di,Zhi Yang,Weichao Chou,Buchang Shi
As a support for cobalt Fischer-Tropsch synthesis catalyst, graphene needs to be pretreated to modify its surface properties for desired catalytic performance. Graphene was pretreated by thermal reflux using 20–60 % nitric acid. The pretreated graphene and catalysts were characterized with TPR, TEM, SEM, XRD, XPS and Raman spectroscopy to study the effect of treatment on microstructures of the materials. This work showed that cobalt metal uniformly dispersed on graphene, and with increasing pretreating acid concentration, surface defects increased and the cobalt particle size decreased, suggesting alleviated agglomeration of cobalt particles on graphene. Pretreating improved the reduction of the catalyst due to the increased defect sites, improved support-metal interaction and cobalt dispersion. Pretreated catalyst yielded better activity, higher C 5+ , but lower methane selectivity than unpretreated catalyst. Therefore, acid pretreatment of graphene can be an effective approach to modify the microstructure of the material, and can thus enhance the performance of the prepared catalyst.
研究领域:
催化化学
·
材料科学
✓ 已验证
DOI
高影响因子
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
·
2025
·
IF 3.3
Yu Xiaohong,Zhang Ya,Wang Xianhao,Luo Yutong,Shao Shuai,Qiu Zhongyang
Organic acid pretreatment is one of the most promising methods to pretreat lignocellulose biomass due to its mild condition and weak corrosion to reactor. In this study, five different organic acids were used for wheat bran pretreatment, and the enzymatic hydrolysis yields of pretreated feedstocks were evaluated. Among these organic acids, oxalic acid pretreatment demonstrated the highest glucose yield of 87.93%. Subsequently, the optimization of oxalic acid pretreatment parameters was conducted including temperature, time, solid–liquid ratio, and acid usage. A high glucose yield of 96.92% was obtained at the optimal conditions: 0.8% of oxalic acid usage, 1:10 of solid–liquid ratio, and 130 ℃ for 15 min. FTIR, XRD and SEM revealed the mechanism of improved hydrolysis efficiency after oxalic acid pretreatment. Finally, the pretreated wheat bran was used for separate hydrolysis and ethanol fermentation (SHF) at 20% (w/w) solids loading. After 36 h, 23.87 g/L ethanol was produced by Saccharomyces cerevisiae DQ1 with all glucose consumed, with the yield of 0.12 g/g dry virgin wheat bran. This study provided a new insight into wheat bran pretreatment for biofuel production by a mild oxalic acid pretreatment method.
研究领域:
生物技术
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可再生能源
·
微生物学
✓ 已验证
DOI
PubMed
高影响因子
BIOTECHNOLOGY PROGRESS
·
2025
·
IF 2.5
Xin Tan,Xuan Wu,Wei Wang,Jiale An,Qin Zhang,Song Tang,Bangxiang He,Chenhuan Lai,Yequan Sheng
The inhibition of lignin condensation during biomass pretreatment is crucial for enhancing enzymatic hydrolysis efficiency, since the formation of rigid cross-linked lignin networks hinders cellulose accessibility and enzyme activity. This study investigates the effects of nucleophilic additives, including ascorbic acid (AsA), 2-naphthol (2N), 3-hydroxy-2-naphthoic acid (3H2NA), and 2-naphthol-7-sulfonate (7S2NA), as potential agents to suppress lignin condensation on the phosphoric acid pretreatment of poplar. The phosphoric acid pretreatment demonstrated a remarkable efficacy in the removal of xylan (100%) and lignin (18.06%–31.35%) from poplar, both with and without the inclusion of nucleophilic additives. An enzymatic hydrolysis yield ranging from 71.41% to 100% was achieved with the incorporation of AsA, 2N, 3H2NA, and 7S2NA, compared to a yield of 66.15% for substrates pretreated solely with phosphoric acid. The enhancement in enzymatic hydrolysis yield upon the addition of nucleophilic additives was probably due to the improved cellulose accessibility and the enhanced proportion of cellulose II in the pretreated substrates. The analysis of total phenolic content in the prehydrolysates revealed that 3H2NA and 7S2NA, characterized by their strong hydrophilic groups within their chemical structures, significantly facilitated lignin fractionation during phosphoric acid pretreatment.
研究领域:
生物化学
·
可再生能源
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
·
2022
·
IF 11.889
Xuelian Zhou,Chunlong Guan,Yexuan Xu,Shilong Yang,Chen Huang,Jiulong Sha,Hongqi Dai
The anatomical and histochemical characterization of pretreated substrates is essential for the further valorization of biomass during the biorefinery process. In this work, the benzenesulfonic acid (BA)-treated substrates were employed for simultaneous saccharification and fermentation (SSF) of ethanol for the first time. An ethanol yield of 50.36% was attained at 10% solids loading and 47.45 g/L of ethanol accumulated at 30 % solids loading. The dramatic improvements could result from the deconstruction of cell walls, which were evidenced by fluorescence microscope and confocal Raman microscopy spectra. Additionally, for a thorough comprehension of the inherent chemistry of lignin during the BA pretreatment, the changes in lignin structure features were identified for the first time by gel permeation chromatography (GPC) and nuclear magnetic resonance (NMR). In summary, this study tried to probe the possibility of BA-treated Miscanthus for the SSF process and unveiled the mechanism of the efficient BA pretreatment.
研究领域:
生物能源
·
植物学
✓ 已验证
DOI
PubMed
高影响因子
Biomass Conversion and Biorefinery
·
2020
·Vol. 13
·期 2
·647-660
·
IF 2.602
Nalawade Ketaki,Saikia Paharika,Behera Shuvashish,Konde Kakasaheb,Patil Sanjay
The bioprospecting of sugarcane bagasse (SCB) through alkali, acid, and hydrodynamic cavitation pretreatment methods and their combinations were evaluated based on bagasse composition, enzymatic hydrolysis, and lactic acid productivity using Bacillus coagulans NCIM 5648. From 100.0 g of SCB, L-lactic acid production of 26.16 g, 8.78 g, 14.15 g, 14.33 g, and 24.61 g in alkali, acid, sequential acid-alkali, sequential alkali-acid, and cavitation with alkali pretreatment was obtained, respectively. Considering the holistic approach from SCB to L-lactic acid, alkali pretreatment is found to be the best method with L-lactic acid titer of 68.7 g/L, the productivity of 2.86 g/L/h, and yield of 0.92 g/g which has resulted in 82.5% higher product yield from SCB as compared to alkali-acid pretreatment. Cavitation in presence of alkali evolved as the next better route with L-lactic acid titer of 62.5 g/L, the productivity of 2.60 g/L/h, and yield of 0.92 g/g. Though the highest glucose release of 89.3 g/L was achieved during enzymatic hydrolysis with sequential alkali-acid-pretreated SCB that resulted in the highest L-lactic acid titer of 71.8 g/L, the productivity of 2.99 g/L/h and fermentation yield of 0.90 g/g.
研究领域:
生物技术
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可再生能源
·
发酵工程
✓ 已验证
DOI
高影响因子
Polymers
·
2020
·Vol. 12
·期 1
·191
·
IF 3.426
Xiaolin Luo,Zhenggang Gong,Jinghao Shi,Lihui Chen,Wenyuan Zhu,Yonghui Zhou,Liulian Huang,Jing Liu
A hydrotrope-based pretreatment, benzenesulfonic acid (BA) pretreatment, was used to fractionate bamboo in this work. With optimized content (80 wt %) of BA in pretreatment liquor, about 90% of lignin and hemicellulose could be removed from bamboo under mild conditions (95 °C, 30 min or 80 °C, 60 min). The potential accessibility of BA pretreated substrate to cellulase was thus significantly improved and was also found to be much higher than those of acidic ethanol and dilute acid pretreatments. But the deposition of lignin on the surface of solid substrates, especially the BA pretreated substrate, was also observed, which showed a negative effect on the enzymatic hydrolysis efficiency. The addition of inexpensive soy protein, a bio-based lignin-shielding agent, could readily overcome this negative effect, leading the increase of enzymatic conversion of cellulose in BA pretreated substrate from 37% to 92% at a low cellulase loading of 4 FPU/g glucan. As compared to acidic ethanol and dilute acid pretreatments, the combination of BA pretreatment and soy protein could not only stably improve the efficiency of non-cellulose components removal, but also could significantly reduce the loading of cellulase.Keywords:benzenesulfonic acid pretreatment;components removal;lignin deposition;enzymatic hydrolysis;lignin-shielding agent
研究领域:
生物化学
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生物质能
·
可再生能源
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）