TALANTA
·
2024
·
IF 5.6
Zihan Wang,Yang Liu,Meiqi Liang,Yihong Chen,Wenjuan Dong,Qin Hu,Shengmei Song,Shaomin Shuang,Chuan Dong,Xiaojuan Gong
Chinese Baijiu (Liquor) is a popular alcoholic beverage , and the ethanol content in Baijiu is closely related to its quality; therefore, it is of great significance to explore a facile, sensitive, and rapid method to detect ethanol content in Baijiu. Hydrophobic carbon quantum dots (H-CQDs) with bright red fluorescence (24.14 %) were fabricated by hydrothermal method using o -phenylenediamine, p -aminobenzoic acid, manganese chloride, and hydrochloric acid as reaction precursors. After the introduction of ultrapure water into the ethanol solution dissolved with H-CQDs, the aggregated H-CQDs resulted in significant changes in fluorescence intensity and absorbance. On this basis, a sensor for detecting ethanol by optical dual-mode and smartphone imaging was constructed. More importantly, the sensor can be used for detecting ethanol content in Chinese Baijiu with satisfactory results. This sensing platform has great potential for quality identification in Chinese Baijiu, broadening the application scope of CQDs in food safety detection.
研究领域:
食品安全检测
·
化学传感器
·
纳米材料
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Foods
·
2024
·Vol. 13
·期 11
·1730
·
IF 4.7
Zhe Liu,Xiaojie Ma,Yanzheng Ge,Xue Hei,Xinyu Zhang,Hui Hu,Jinjin Zhu,Benu Adhari,Qiang Wang,Aimin Shi
Microfluidic technology, as a continuous and mass preparation method of nanoparticles, has attracted much attention in recent years. In this study, zein nanoparticles (ZNPs) were continuously fabricated in a highly controlled manner by combining a microfluidics platform with the antisolvent method. The impact of ethanol content (60~95%,v/v) and flow rates of inner and outer phases in the microfluidics platform on particle properties were examined. Among all ZNPS, 90%-ZNPs have the highest solubility (32.83%) and the lowest hydrophobicity (90.43), which is the reverse point of the hydrophobicity of ZNPs. Moreover, when the inner phase flow rate was 1.5 mL/h, the particle size decreased significantly from 182.81 nm to 133.13 nm as the outer phase flow rate increased from 10 mL/h to 50 mL/h. The results revealed that ethanol content had significant impacts on hydrophilic–hydrophobic properties of ZNPs. The flow rates of ethanol–water solutions and deionized water (solvent and antisolvent) in the microfluidics platform significantly affected the particle size of ZNPs. These findings demonstrated that the combined application of a microfluidics platform and an antisolvent method could be an effective pathway for precisely controlling the fabrication process of protein nanoparticles and modulating their physicochemical properties.
研究领域:
食品科学
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纳米技术
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生物工程
✓ 已验证
DOI
PubMed
高影响因子
ACTA PHARMACOLOGICA SINICA
·
2012
·Vol. 33
·期 7
·909-917
·
IF 1.953
Wang Xin-ting,Li Jia,Liu Li,Hu Nan,Jin Shi,Liu Can,Mei Dan,Liu Xiao-dong
Aim: Diabetes is associated with elevated serum total cholesterol level and disrupted lipoprotein subfractions. The aim of this study was to examine alterations in the tissue cholesterol contents closely related to diabetic complications. Methods: Intraperitoneal injection of streptozotocin was used to induce type 1 diabetes in adult male Sprague-Dawley rats. On d 35 after the injection, liver, heart, intestine, kidney, pancreas, cerebral cortex and hippocampus were isolated from the rats. The content of total and free cholesterol in the tissues was determined using HPLC. The ATP-binding cassette protein A1 (ABCA1) protein and ApoE mRNA were measured using Western blot and QT-PCR analyses, respectively. Results: In diabetic rats, the level of free cholesterol was significantly decreased in the peripheral tissues, but significantly elevated in hippocampus, as compared with those in the control rats. Diabetic rats showed a trend of decreasing the total cholesterol level in the peripheral tissues, but significant change was only found in kidney and liver. In diabetic rats, the level of the ABCA1 protein was significantly increased in the peripheral tissues and cerebral cortex; the expression of ApoE mRNA was slightly decreased in hippocampus and cerebral cortex, but the change had no statistical significance. Conclusion: Type 1 diabetes decreases the free cholesterol content in the peripheral tissues and increases the free cholesterol content in hippocampus. The decreased free cholesterol level in the peripheral tissues may be partly due to the increased expression of the ABCA1 protein.
研究领域:
内分泌学
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代谢学
·
药理学
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神经科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHINESE JOURNAL OF CHEMICAL PHYSICS
·
2024
·Vol. 37
·期 4
·563-570
·
IF 1.2
Shi Hehang,Ye Xiaodong
Two soy protein 11S fractions with different surface sulfhydryl contents were prepared. Utilizing analytical ultracentrifugation, the effects of storage time and hydrogen peroxide at different concentrations (0.5—100 mmol/L) on the two 11S fractions were investigated. Results show that after removing 2-mercaptoethanol (2-ME) by size exclusion chromatography, the 11S fraction with high surface sulfhydryl content (2.0 mol sulfhydryl/mol 11S) progressively formed 15S and 21S in dilute solutions during storage at 4 °C for 82 days. While, the 11S fraction with low surface sulfhydryl content (0.2 mol sulfhydryl/mol 11S) was stable under the same condition. Moreover, after treating the 11S with high surface sulfhydryl content with 1 mmol/L H 2 O 2 , the weight percentage of 15S reached the maximum value of 20%. The 15S induced by air and H 2 O 2 could be totally converted to 11S with the addition of 10 mmol/L 2-ME, which could be attributed to that the disulfide bond linking two 11S molecules is on the surface of the 15S and easily accessible to the reducing agent 2-ME. This study helps us to deeply understand the formation mechanism of 15S and the stability of 11S.
研究领域:
食品科学
·
蛋白质化学
·
分析化学
✓ 已验证
DOI
高影响因子
Food Bioscience
·
2024
·
IF 4.8
Xiaohan Wei,Jie Hao,Kexin Xiong,Hui Guo,Siyu Xue,Yiwei Dai,Yujiao Zhang,Yingxi Chen,Sufang Zhang
Kiwifruit, a climacteric-fruit susceptible to rapid deterioration, attains considerable value upon transformation into kiwifruit wine, thereby increasing its added value. However, a noteworthy challenge arises from its high pectin content, which negatively affects byproduct quality, necessitating pectinase treatment during processing. Commercial pectinases exhibit heightened pectin esterase activity, giving rise to safety apprehensions due to augmented methanol content during kiwifruit pectin hydrolysis. In this study, Bacillus velezensis W17-6 was screened for its high pectinase activity coupled with low pectin esterase activity. The resultant crude pectinase named PBV was acquired through fed-batch-fermentation and partial purification. Subsequently, PBV was deployed in the fermentation of Xuxiang kiwifruit wine, and its impact on overall quality was assessed through diverse analyses. The results indicated that Xuxiang kiwifruit wine with PBV treatment outperformed counterparts treated with commercial pectinases. PBV treatment not only yielded superior clarity (83.75% ± 0.47%) and juice yield (81.60% ± 0.47%) but also increased the total flavonoids content in Xuxiang kiwifruit wine by 1.2 times compared to commercial pectinase. Notably, the methanol content of PBV-treated Xuxiang kiwifruit wine was significantly lower at 36.13 ± 1.83 mg/L than that of wines treated with two commercial pectinases. PBV-treated kiwifruit wine also exhibited elevated concentrations of volatile compounds such as phenethyl alcohol, ethyl undecanoate, lauric aldehyde, isobutyric acid, and myrcene. Impressively, PBV-treated kiwifruit wine surpassed other wines in both electronic and artificial sensory analyses. In summary, PBV exhibits substantial potential in the production of low-methanol, high-quality kiwifruit wine, enhancing the flavor and nutritional value while minimizing methanol production and improving the safety profile.
研究领域:
食品科学
·
酿酒学
·
生物技术
·
微生物学
✓ 已验证
DOI
高影响因子
TREE PHYSIOLOGY
·
2017
·Vol. 37
·期 12
·1659-1671
·
IF 3.653
Yu Chunna,Guo Hong,Zhang Yangyang,Song Yaobin,Pi Erxu,Yu Chenliang,Zhang Lei,Dong Ming,Zheng Bingsong,Wang Huizhong,Shen Chenjia
Taxol is currently a valuable anticancer drug; however, the accumulated mixture of taxoids can vary greatly among Taxus species. So far, there is very little genomic information for the genus Taxus, except for Taxus baccata. Transcriptome analysis is a powerful approach to explore the different regulatory mechanisms underlying the taxoid biosynthesis pathway in Taxus species. First, we quantified the variation in the taxoid contents between Taxus media and Taxus mairei. The contents of paclitaxel and 10-deacetylpaclitaxel in T. media are higher than that in T. mairei. Then, the transcriptome profiles of T. media and T. mairei were analyzed to investigate the altered expressions. A total of 20,704 significantly differentially expressed genes (DEGs), including 9865 unigenes predominantly expressed in T. media and 10,839 unigenes predominantly expressed in T. mairei, were identified. In total, 120 jasmonic acid-related DEGs were analyzed, suggesting variations in ‘response to JA stimulus’ and ‘JA biosynthetic process’ pathways between T. media and T. mairei. Furthermore, a number of genes related to the precursor supply, taxane skeleton formation and hydroxylation, and C13-side chain assembly were also identified. The differential expression of the candidate genes involved in taxoid biosynthetic pathways may explain the variation in the taxoid contents between T. media and T. mairei.
研究领域:
植物科学
·
药理学
·
分子生物学
·
基因组学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MATERIALS SCIENCE FORUM
·
2015
·
IF 0
Wang Fa Xu,Fa Meng Sun,Jing Bo Liu,Xiao Fang Li,Fa Ai Zhang
A series of porous poly(styrene-divinylbenzene) monoliths (polyHIPE) was synthesized by high internal phase emulsions (HIPE). The effects of the ethanol contents on the microstructure of the polyHIPE were characterized by scanning electron microscopy and mercury intrusion porosimeter. The adsorption and release behaviors of the polyHIPE for a monomer mixture consisting of dicyclopentadiene (DCPD) /styrene (St) (DS, mass ratio of 9/1) were investigated. The polymerization of the mixture DS within pores of the polyHIPE was studied. The results showed that the microstructure of the polyHIPE was significantly influenced by the ethanol content. The increase of ethanol in the HIPE increased the internal pore diameter and decreased the pore volume of the polyHIPE, thus decreased the DS absorption and increased the release rate. The DS monomer can enter the pores(cavities, windows) and fill the original holes after the polymerization. Compared with conventional polymerization of DCPD/St, the poly(DCPD/St) (pDS) obtained from confined spaces possess higher molecular weight, broader molecular weight distribution, and higher Tg. As the pore diameter increased, the effect of space confinement reduced, and the performances of pDS were closed to that prepared by conventional polymerization.
研究领域:
材料科学
·
化学工程
·
高分子化学
✓ 已验证
DOI
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
·
2024
·
IF 3.8
Peiyu Xue,Xinyong You,Li Ren,Weiming Yue,Zheng Ma
Kaempferol can exert biological functions by regulating various signaling pathways. This study evaluated the ameliorative effect of kaempferol on lipid accumulation using oleic acid and palmitic acid-treated HepG2 cells and high-fat diet mice. In vitro oil red O staining showed that kaempferol treatment improved lipid accumulation ( p < 0.001 for TG content and p < 0.05 for TC content). Immunofluorescence, Western blot analysis and RT-qPCR showed that kaempferol could promote nuclear translocation of PPARγ and reduce the expression of PPARγ, C/EBPβ, and SREBP-1c. Dietary intervention with kaempferol could reduce the lipid accumulation in hepatocytes and inflammatory cell infiltration, as well as attenuated serum levels of IL-6 and TNF-α in HFD-fed mice ( p < 0.001 for IL-6 and p < 0.01 for TNF-α at kaempferol 60 mg/kg/d). Meanwhile, histopathological examination revealed that there was no substantial damage or distinct inflammation lesions in organs at the experimental dose, including the heart, lung, kidney, and spleen. The aforementioned research findings can serve as references for further preclinical investigations on the potential of kaempferol to mitigate lipid accumulation.
研究领域:
药理学
·
分子生物学
·
营养学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Yifan Zhu,Mubango Elliot,Yanhong Zheng,Jing Chen,Dongzhi Chen,Shanggui Deng
Atmospheric cold plasma (ACP) is a novel nonthermal technology with potential applications in maintaining and improving food quality. The effect of ACP on the activity and structure of mushroom ( Agaricus bisporus ) polyphenol oxidase (PPO) was evaluated. Results demonstrated that the dielectric barrier discharge (DBD) based plasma technology could inactivate PPO (up to 69%) at 50 kV with the increased concentrations of H 2 O 2 and NO x . An obvious enhancement of surface hydrophobicity was observed, whereas a gradual reduction of total sulfhydryl content was recorded with the increasing exposure time. Data from circular dichroism, atomic force microscopy, particle size distribution and fluorescence spectra displayed the rearrangement of secondary structure and disruption of the tertiary structure. Red shifts of fluorescence spectra showed positive correlations with the inactivation rate of PPO. Therefore, ACP treatment could be served as an alternative approach to inactivate undesirable enzymes to minimize the loss of food nutrition and quality.
研究领域:
食品科学
·
生物技术
·
酶学
·
等离子体物理
✓ 已验证
DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
·
2021
·
IF 9.147
Yajuan Sun,Saiying Fan,Rong Liang,Xinjiong Ni,Yangbiao Du,Jing Wang,Cheng Yang
A hybrid microcapsule with modified starch shell and solid lipids core was designed to enhance the thermal stability of menthol with high loading. The hybrid microcapsule was prepared by high-pressure homogenization of the lipid and water phase, and spray drying of the modified starch stabilized emulsion. Menthol was incorporated with the oil phase into the modified starch shell. The crystalline structure of menthol in solid lipids and the hybrid microcapsules was investigated by X-ray diffraction (XRD) and differential scanning calorimetry (DSC) analysis. The encapsulation efficiency, surface menthol content, loading amount, and thermal stability were optimized. A hybrid microcapsule with a high encapsulation efficiency (99.78%), low surface oil content (3.02%), high loading amount (31.18%) and superb thermal stability was obtained. The microstructure of the hybrid microcapsule was studied by scanning electron microscope (SEM) and confocal laser scanning microscope (CLSM). The mechanism of superb thermal stability and high loading was discussed, both of the amorphous form of menthol and the dense modified starch shell are important. Finally, the shelf storage stability for 300 days was evaluated. In general, a simple and general method was provided to stabilize menthol at high temperatures. And the microencapsulation concept proposed in this manuscript may provide a potential idea for other crystalline active ingredients used in food, cosmetic and pharmaceutical area.
研究领域:
材料科学
·
药剂学
·
食品科学
·
化学工程
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）