Infection and Drug Resistance
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2022
·
IF 4.177
Jie Zhu,Yibing Huang,Mingxia Chen,Cuihua Hu,Yuxin Chen
Background and purpose To reduce the resistance and allergic reaction to chlorhexidine acetate (CHA) in the current treatment of (Bacterial vaginosis) BV and (vulvovaginal candidiasis) VVC in female vaginitis. In this study, the antimicrobial activities and mechanism of action of the synergistic effects of antimicrobial peptides (AMPs) HPRP-A1 and HPRP-A2, and CHA, against Gram-negative and Gram-positive bacteria, and one fungus Candida albicans (C. albicans) were investigated in vitro and in mouse and rat vaginitis infection models in vivo.Results HPRP-A1, HPRP-A2 and CHA showed significant synergistic effects on the antimicrobial activities against different Gram-negative and Gram-positive bacteria and C. albicans. The combined application of HPRP-A2 and CHA exhibited strong synergistic effects in the mouse and rat vaginitis models caused by bacteria or C. albicans.Conclusion This study may prompt the development of new drug combinations against vaginitis infections, including mixed bacterial and fungal infections and multi-drug-resistant infections.
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Journal of Functional Biomaterials
·
2022
·Vol. 13
·期 1
·24
·
IF 4.901
Wenlong Li,Guanping Hua,Jingfeng Cai,Yaming Zhou,Xi Zhou,Miao Wang,Xiumin Wang,Baoqing Fu,Lei Ren
Antibacterial coating with antibiotics is highly effective in avoiding device-associated infections (DAIs) which is an unsolved healthcare problem that causes significant morbidity and mortality rates. However, bacterial drug resistance caused by uncontrolled release of antibiotics seriously restricts clinical efficacy of antibacterial coating. Hence, a local and controlled-release system which can release antibiotics in response to bacterial infected signals is necessary in antibacterial coating. Herein, a multi-stimulus responsive multilayer antibacterial coating was prepared through layer-by-layer (LbL) self-assembly of montmorillonite (MMT), chlorhexidine acetate (CHA) and Poly(protocatechuic acid-polyethylene glycol 1000-bis(phenylboronic acid carbamoyl) cystamine) (PPPB). The coating can be covered on various substrates such as cellulose acetate membrane, polyacrylonitrile membrane, polyvinyl chloride membrane, and polyurethane membrane, proving it is a versatile coating. Under the stimulation of acids, glucose or dithiothreitol, this coating was able to achieve controlled release of CHA and kill more than 99% of Staphylococcus aureus and Escherichia coli (4 × 108CFU/mL) within 4 h. In the mouse infection model, CHA releasing of the coating was triggered by infected microenvironment to completely kill bacteria, achieving wounds healing within 14 days.Keywords:device-associated infections;multi-stimulus responsiveness;antibacterial coating;wound healing
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Yushuang Shang,Penghui Wang,Xiuzhen Wan,Lijuan Wang,Xu Liu,Jiang Yuan,Bo Chi,Jian Shen
Multifunctional hydrogel dressings are promising for wound healing. In the study, chlorhexidine(CHX) loaded double network hydrogels were prepared by free radical polymerization of sulfobetaine and oxidative self-crosslinking of reduced keratin. The introduced keratin and CHX endowed hydrogels with cytocompatibility, antioxidant capability as well as enhanced antibacterial activity due to the antifouling property of polysulfobetaine. These hydrogels exhibited acidity, glutathione(GSH), and trypsin triple-responsive release behaviors, resulting in the accelerated release of CHX under wound microenvironments. Intriguingly, the freeze-drying hydrogels could be ground to powders and sprinkled on the irregular wound bed, followed by absorbing wound fluid to reform hydrogel in situ. These xerogel powders were more convenient for sterilization, formulation, and storage. Further, these xerogel powders could be rejected after being mixed with an appropriate amount of water. In vivo infected wound healing confirmed that the xerogel powder dressing significantly promoted collagen deposition and reduced inflammation, thereby accelerating the closure and regeneration of skin wounds. Taken together, these degradable xerogel powders have great potential applications for wound healing.
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RSC Advances
·
2015
·Vol. 5
·期 100
·82334-82341
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IF 3.84
Xuelian Zhi,Yanfang Wang,Pengfei Li,Jiang Yuan,Jian Shen
Nanoscale polyion complex formation via the electrostatic complexation of a polyelectrolyte and a charged drug is the most convenient method for building a drug delivery system that simultaneously realizes the carrier preparation and drug embedding. Herein, we prepared keratin/chlorhexidine complex nanoparticles (KCNPs) based on a drug-induced ionic gelation technique without using a crosslinker, organic solvent or surfactant. These KCNPs exhibited good stability even after having been long-standing for 14 days. The KCNPs were characterized using FTIR, DLS, SEM and TEM. It was found that these nanoparticles had a spherical morphology with a diameter of about 180 nm, and a negatively charged surface with a zeta potential of about −39.1 mV. The cell toxicity of the KCNPs at different dosage levels was evaluated using the MTT assay method, indicating their slight cytotoxicity at lower dosages. The antibacterial activity against E. coli and S. aureus was determined using the zone of inhibition method. It seemed that the KCNPs had better antibacterial activity against S. aureus than against E. coli. Drug delivery profiles showed that the chlorhexidine (CHX) loaded nanoparticles exhibited both pH- and glutathione-responsive character. These keratin-based complex nanoparticles can be regarded as a valuable stimuli-responsive strategy for the delivery of anticancer agents.
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Gels
·
2025
·Vol. 11
·期 2
·128
·
IF 5.3
Shuai Gao,Wei Zeng,Zheng Liu,Fanjun Zhang,Yunfeng Zhang,Xi Liu,Dimeng Wu,Yunbing Wang
Silicone urinary catheters are broadly employed in medical practice. However, they are susceptible to inducing catheter-associated urinary tract infections (CAUTIs) due to bacterial adherence to the catheter’s surface, and they exhibit a high friction coefficient, which can greatly affect their effectiveness and functionality. Thus, the development of a silicone urinary catheter with antibacterial properties and lubricity is in strong demand. We hereby developed a poly(vinyl acetate) carrier coating to load chlorhexidine acetate and applied a hydrogel coating primarily composed of polyvinylpyrrolidone (PVP) and poly(ethylene glycol) diacrylate (PEGDA), which was then coated onto the silicone urinary catheters and cured through a thermal curing process and could provide lubricity. Subsequently, we analyzed its surface characteristics and assessed the antibacterial property, lubricity, cytotoxicity, and potential for vaginal irritation. The findings from the Fourier transform infrared spectrometer (FTIR), scanning electron microscope (SEM), water contact angle (WCA), inhibition zone measurements, and friction coefficient analysis confirmed the successful modification of the silicone urinary catheter. Additionally, the outcomes from the cytotoxicity and vaginal irritation assessments demonstrated that the dual-function hydrogel coating-coated silicone urinary catheters exhibit outstanding biocompatibility. This study illustrates that the prepared silicone urinary catheters possess durable antibacterial properties and lubricity, which thus gives them broad clinical application prospects.
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Biomaterials Science
·
2018
·Vol. 6
·期 3
·596-603
·
IF 5.831
Tengyi Zheng,Xintian Huang,Jintian Chen,Disang Feng,Liling Mei,Ying Huang,Guilan Quan,Chune Zhu,Vikramjeet Singh,Hao Ran,Xin Pan,Chuan-Yu Wu,Chuanbin Wu
Lyotropic liquid crystals (LLC) have received increasing attention as a drug delivery system. In this study, a novel intra-canal disinfectant based on the glycerol monooleate (GMO) LLC precursor incorporation with chlorhexidine (CHX) and silver nanoparticles (Ag-NPs) was designed and evaluated. The LLC precursor with excellent fluidity was able to penetrate deeply into the complex tiny collateral branch root canals. The transformation of cubic LLC in root canals upon coming into contact with water provided long-lasting disinfection against multidrug-resistant bacteria to avoid the endodontic reinfection and follow-up visits. The GMO–ethanol–water (48% : 12% : 40%, w/w) formulation containing 0.5% CHX and 0.02% Ag-NPs was selected for further studies. The low viscosity of the precursor presented excellent injectability and flowabilities. From the in vitro release test, the release behaviours were found to be influenced by CHX and Ag-NP contents, allowing the optimized precursor to obtain a 28-day release profile. The CHX-Ag-NP containing LLC precursor exhibited an excellent and sustained sterilization effect on Enterococcus faecalis for more than one month with a bacterial inactivation rate of ≥98.5%, which was far more than the minimum clinical requirement (7 days). Furthermore, no in vitro toxicity was observed in the cytotoxicity evaluation. The CHX-Ag-NP containing LLC precursor was proved to be a promising intra-canal disinfectant in our study.
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Frontiers in Microbiology
·
2025
·
IF 4.5
Zibei Wu,Xufeng Zhang,Lili Wang,Jinlei Sui,Mingqi Yu,Bingzhangke Bao,Jianing Qian,Guohui Yi,Yinzheng Ma,Xiaowen He
Streptococcus mutans is one of the most significant pathogens leading to dental caries by producing highly cariogenic biofilms within the oral cavity. However, the increasing antimicrobial resistance brings great difficulties to the prevention and treatment of dental caries. Therefore, it is very important to develop new and effective antimicrobial agents from natural sources. Clausena lansium (Lour.) Skeels, a plant commonly cultivated in Southern China, is rich in various active constituents. In this study, the main constituents of the essential oils extracted from Clausena lansium leaves (EOL), seeds (EOS), peels (EOP) were precisely analyzed by gas chromatography–mass spectrometry. The antimicrobial and anti-biofilm activities of EOL, EOS, and EOP, along with their main chemical constituents, against Streptococcus mutans were comprehensively evaluated. The primary constituent in EOL was β-caryophyllene, with a content of 112.29 mg/mL. EOS was found to be abundant in sabinene and 4-terpineol, with constituents of 147.21 mg/mL and 54.36 mg/mL, respectively. In EOP, the most predominant constituent was β-phellandrene, with a content of 173.95 mg/mL. The minimum inhibitory concentrations of EOL, EOS, and EOP were 0.12, 2.00, and 1.00 mg/mL, respectively. The minimum concentrations of inhibiting at least 90% biofilm formation of EOL, EOS, and EOP was 0.12 mg/mL, 2.00, and 2.00 mg/mL, respectively. Intriguingly, the antimicrobial and anti-biofilm efficacies of the main constituents were significantly lower than those of the essential oils. By observing the dynamic changes of the biofilm formation of S. mutans treated with essential oils, it was found that the process of biofilm formation was greatly delayed. In some cases, there was even no biofilm formation. The results of real-time quantitative PCR indicated that the expression levels of brpA, gtfB, gtfC, gtfD, and luxS genes were down-regulated after treatment with the essential oils. This study reinforces the potential of Clausena lansium as a promising source for developing new antimicrobial and anti-biofilm agents. They could inhibit the proliferation and virulence of S. mutans, thereby contributing to the control of dental caries.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Mohan Hou,Qiuyu Xu,Lifang Wang,Xuepeng Zhang,Lei Zhang,Hongguo Gao,lifang Liu
Maintaining a moderate moist microenvironment and establishing an effective antimicrobial barrier are of great significance for promoting wound healing. However, traditional dressing materials often encounter bacterial infections, and the system design defects lead to weak moisturizing ability, and the rapid water evaporation rate leads to premature dehydration of the wound, which is not conducive to wound healing. The synergy between the microscopic wettability control and the macrostructure design of the material is crucial. In this work, nanocelluloses (TCNCs) /sodium alginate (SA) were used as substrates for porous materials due to their good biocompatibility and wettability. The internal structure of TCNCs/SA were optimized by changing the ratio of SA to TCNCs. The excellent pore structure makes the samples have better water retention ability and moisture vapor transmittance (water retention ratio = 20.9, MVTR = 1412.37 g/m 2 /d). Besides, the loading of chlorhexidine acetate (CHX) can effectively improve the antibacterial properties of TCNCs/SA and the antibacterial diameter against Escherichia coli and Staphylococcus aureus is >10 mm. Finally, the TNSX sample caused considerable acceleration at the SD rats skin excisions healing. The results illustrate that the TCNCs/SA porous foams have great potential application in medical dressings.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Shaohui Guo,Minxuan Yang,Zhilang Xu,Liming Ge,Changdao Mu,Defu Li
Guided bone regeneration (GBR) technique is a standard therapy for reconstructing alveolar bone defects clinically, with the GBR membrane serving as the key medical device in this technique. However, the most commonly used collagen barrier membranes usually have poor mechanical properties and are prone to collapse during clinical use. Herein, a sandwich-like barrier membrane was developed with crosslinked chitosan as dense layer, crosslinked collagen as loose layer and passivated magnesium mesh as the skeleton. Surface modification has improved the corrosion resistance of magnesium mesh. Thanks to the introduction of magnesium mesh skeleton and crosslinking modification, the mechanical properties and supporting effects of barrier membrane have been improved. The dense layer of barrier membrane showed dense structure while the loose layer showed porous structure as expected. The barrier membrane showed good antibacterial performance against gram-positive and gram-negative bacteria owing to the antibacterial activity of chitosan and the added chlorhexidine acetate. Furthermore, the barrier membrane met the requirements of biocompatibility for implanted biomaterials. Interestingly, the barrier membrane had good anti-inflammatory activity to accelerate the transition from inflammatory to proliferative stage and can promote osteogenic differentiation of MC3T3-E1 cells. Overall, the sandwich-like barrier membrane has a wide application prospect to guide bone regeneration.
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