eLife
·
2025
·
IF 0
Jing Lu,Hao Xu,Dongyue Wang,Yanlu Chen,Takeshi Inoue,Liang Gao,Kai Lei
The intricate coordination of the neural network in planarian growth and regeneration has remained largely unrevealed, partly due to the challenges of imaging the CNS in three dimensions (3D) with high resolution and within a reasonable timeframe. To address this gap in systematic imaging of the CNS in planarians, we adopted high-resolution, nanoscale imaging by combining tissue expansion and tiling light-sheet microscopy, achieving up to fourfold linear expansion. Using an automatic 3D cell segmentation pipeline, we quantitatively profiled neurons and muscle fibers at the single-cell level in over 400 wild-type planarians during homeostasis and regeneration. We validated previous observations of neuronal cell number changes and muscle fiber distribution. We found that the increase in neuron cell number tends to lag behind the rapid expansion of somatic cells during the later phase of homeostasis. By imaging the planarian with up to 120 nm resolution, we also observed distinct muscle distribution patterns at the anterior and posterior poles. Furthermore, we investigated the effects of β-catenin-1 RNAi on muscle fiber distribution at the posterior pole, consistent with changes in anterior-posterior polarity. The glial cells were observed to be close in contact with dorsal-ventral muscle fibers. Finally, we observed disruptions in neural-muscular networks in inr-1 RNAi planarians. These findings provide insights into the detailed structure and potential functions of the neural-muscular system in planarians and highlight the accessibility of our imaging tool in unraveling the biological functions underlying their diverse phenotypes and behaviors.
研究领域:
再生生物学
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神经科学
·
显微成像技术
✓ 已验证
产品关联
DOI
PubMed
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Guochen Xia,Shixu Yu,Zezhou Yang,Jingyi Lian,Yimin Xie,Qinghua Feng
Natural wood has been highly valued for thousands of years due to its excellent strength, low density, and ease of processing. However, its poor water stability, which leads to swelling and deformation, limits its competitiveness. In response, we designed a reconstructed wood through a process involving pretreatment, TEMPO oxidation, lignin self-assembly, lignin melting, and densification. In this material, cellulose serves as the skeleton, while lignin with a phenylpropyl structure acts as both a filler and binder. The resulting wood exhibits outstanding water stability, with no changes after 60 days of water immersion and a water absorption rate as low as 8.32 %. This stability is achieved through the densification of lignin, which is enhanced by lignin melting and pressure during self-assembly. The reconstructed wood not only offers excellent water stability but also boasts superior UV resistance and thermal stability. Additionally, it can be hot-pressed into straws within a mold, providing better water stability and tensile strength compared to paper straws. This reconstructed wood, made from natural wood and corncob, is biodegradable and environmentally friendly, offering a new approach to wood water stability strategies.
研究领域:
材料科学
·
林业科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Physical Chemistry Letters
·
2025
·
IF 4.6
Kaiwen Tan,Jingyu Zhang,Xinghan Liu,Jinming Li,Jun Gong,Yonggang Tong,Shiyun Xiong,Gufei Zhang,Yejun Li
For NiFe-based precatalysts, there is a general consensus that they will undergo reconstruction to generate NiFe (oxy)hydroxides as real active sites during the electrochemical oxygen evolution reaction (OER), but the current understanding of the reconstruction process is still far from satisfactory. Herein, iron phosphates/molybdates on nickel foams (FPmM/NF, m = 5, 7, 10, 20) were prepared via a coprecipitation method, where although both MoO42– and PO43– undergo anion exchange during the activation process, i.e., dual reconstructions, to form NiFe (oxy)hydroxides, they show disparate reconstruction dynamics. By tuning the phosphates/molybdates ratio, the morphology, activity, and activation rate of the formed NiFe (oxy)hydroxides can be tuned. Consequently, the obtained optimal FP7M/NF-activated (FP7M/NF-a) possesses a fast and deep reconstruction with a morphology of nanoparticles with a disordered structure and rich oxygen vacancies, thus exhibiting exceptional OER activity (overpotentials of only 175 and 180 mV at 50 and 100 mA·cm–2) and excellent stability.
研究领域:
电化学
·
材料科学
·
催化
·
能量转换
·
表面重建
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYST
·
2021
·Vol. 146
·期 7
·2348-2357
·
IF 4.616
Chuanzhen Hu,Xianli Wang,Ling Liu,Chuanhai Fu,Kaiqin Chu,Zachary J. Smith
Raman hyperspectral imaging is a powerful method to obtain detailed chemical information about a wide variety of organic and inorganic samples noninvasively and without labels. However, due to the weak, nonresonant nature of spontaneous Raman scattering, acquiring a Raman imaging dataset is time-consuming and inefficient. In this paper we utilize a compressive imaging strategy coupled with a context-aware image prior to improve Raman imaging speed by 5- to 10-fold compared to classic point-scanning Raman imaging, while maintaining the traditional benefits of point scanning imaging, such as isotropic resolution and confocality. With faster data acquisition, large datasets can be acquired in reasonable timescales, leading to more reliable downstream analysis. On standard samples, context-aware Raman compressive imaging (CARCI) was able to reduce the number of measurements by ∼85% while maintaining high image quality (SSIM >0.85). Using CARCI, we obtained a large dataset of chemical images of fission yeast cells, showing that by collecting 5-fold more cells in a given experiment time, we were able to get more accurate chemical images, identification of rare cells, and improved biochemical modeling. For example, applying VCA to nearly 100 cells’ data together, cellular organelles were resolved that were not faithfully reconstructed by a single cell's dataset.
研究领域:
生物物理学
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光谱学
·
显微成像
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2025
·
IF 6.2
Xinyu Che,Xiangyu Tao,Jianan Chen,Yanbin Feng,Ziheng Cui,Ting Feng,Yunming Fang,Han Wen,Song Xue
Oleate hydratases (Ohys) catalyze the conversion of oleic acid (OA) to 10-(R)-hydroxystearic acid (10-HSA), a compound widely used in the chemical industry. However, the limited activity of Ohys has hindered their broader applications. To address this limitation, we propose a novel strategy for mining highly active Ohys through structure clustering, sequence clustering, and ancestral sequence reconstruction (SSA strategy). Structure clustering via AI-driven protein structure prediction followed by classification enhanced the ability to mine target Ohys. Ancestral enzyme reconstruction was carried out based on mining results from both structure and sequence clustering. This strategy significantly reduces the time and cost of the discovery process. Among the 1304 Ohys screened via SSA, 13 candidates were selected. Seven candidates demonstrated high activity. Ohy 64, identified through structure clustering, exhibited the highest activity. Ancestral enzymes that were reconstructed from structure clustering targets were 3 times more likely to exhibit high catalytic activity than those identified through sequence clustering. Four critical, hydrophobic residues were identified through structure and sequence comparisons between StOhy and targets mined by SSA. Site-directed mutagenesis revealed that these hydrophobic residues conferred varying levels of enzyme activity, confirming that increased hydrophobicity at these positions enhances cofactor FAD binding, thus improving enzyme catalytic efficiency.
研究领域:
生物化学
·
酶工程
·
计算生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Advanced Science
·
2022
·Vol. 9
·期 16
·2105292
·
IF 17.521
Yawen Jiang,Xinyu Wang,Delong Duan,Chaohua He,Jun Ma,Wenqing Zhang,Hengjie Liu,Ran Long,Zibiao Li,Tingting Kong,Xian Jun Loh,Li Song,Enyi Ye,Yujie Xiong
Structural reconstruction is a process commonly observed for Cu-based catalysts in electrochemical CO 2 reduction. The Cu-based precatalysts with structural complexity often undergo sophisticated structural reconstruction processes, which may offer opportunities for enhancing the electrosynthesis of multicarbon products (C 2+ products) but remain largely uncertain due to various new structural features possibly arising during the processes. In this work, the Cu 2 O superparticles with an assembly structure are demonstrated to undergo complicated structure evolution under electrochemical reduction condition, enabling highly selective CO 2 -to-C 2+ products conversion in electrocatalysis. As revealed by electron microscopic characterization together with in situ X-ray absorption spectroscopy and Raman spectroscopy, the building blocks inside the superparticle fuse to generate numerous grain boundaries while those in the outer shell detach to form nanogap structures that can efficiently confine OH − to induce high local pH. Such a combination of unique structural features with local reaction environment offers two important factors for facilitating C−C coupling. Consequently, the Cu 2 O superparticle-derived catalyst achieves high faradaic efficiencies of 53.2% for C 2 H 4 and 74.2% for C 2+ products, surpassing the performance of geometrically simpler Cu 2 O cube-derived catalyst and most reported Cu electrocatalysts under comparable conditions. This work provides insights for rationally designing highly selective CO 2 reduction electrocatalysts by controlling structural reconstruction.
研究领域:
材料科学
·
电化学
·
环境科学
✓ 已验证
DOI
高影响因子
Nano-Micro Letters
·
2024
·Vol. 17
·期 1
·1-15
·
IF 31.6
An Li,Li Jianyi,Sun Yuanmiao,Zhu Jiamin,Seow Justin Zhu Yeow,Zhang Hong,Zhang Nan,Xi Pinxian,Xu Zhichuan J.,Yan Chun-Hua
Demonstrate that the key factor to determine the activity of reconstructed surfaces is the surface chemistry, instead of reconstruction degree using the popular perovskite LaNi 1-x Fe x O 3 oxides as model materials.
Fe content can influence both the surface reconstruction degree, the activation degree, and the activity of reconstructed surfaces.
The oxygen evolution reaction activity of reconstructed catalysts is primarily governed by the chemistry of the reconstructed surface species.
研究领域:
材料科学
·
电催化
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Chenxi Li,Jing Ge,Quanyi Guo,Jiandong Wang,Jiang Wu,Zineng Yan,Zdenko Špitalský,Yong Liu
Breast cancer has become the most diagnosed cancer type, endangering the health of women. Patients with breast resection are likely to suffer serious physical and mental trauma. Therefore, breast reconstruction becomes an important means of postoperative patient rehabilitation. Polyvinyl alcohol hydrogel has great potential in adipose tissue engineering for breast reconstruction. However, its application is limited because of the lack of bioactive factors and poor structural stability. In this study, we prepared biodegradable polylactic acid-glycolic acid copolymer/polycaprolactone/gelatin (PPG) nanofibers. We then combined them with polyvinyl alcohol/collagen to create tissue engineering scaffolds to overcome limitations. We found that PPG fibers formed amide bonds with polyvinyl alcohol/collagen scaffolds. After chemical crosslinking, the number of amide bonds increased, leading to a significant improvement in their mechanical properties and thermal stability. The results showed that compared with pure PVA scaffolds, the maximum compressive stress of the scaffold doped with 0.9 g nanofibers increased by 500 %, and the stress loss rate decreased by 40.6 % after 10 cycles of compression. The presence of natural macromolecular gelatin and the changes in the pore structure caused by nanofibers provide cells with richer and more three-dimensional adsorption sites, allowing them to grow in three dimensions on the scaffold. So, the hydrogel scaffold by reinforcing polyvinyl alcohol hydrogel with PPG fibers is a promising breast reconstruction method.
研究领域:
生物材料学
·
医学工程
·
肿瘤学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Advanced Photonics Nexus
·
2024
·
IF 0
Wenkai Ma,Qian Xue,Yang Yang,Hanqiu Zhang,Daoli Zhang,Xinzheng Lan,Liang Gao,Jianbing Zhang,Jiang Tang
Since the concept of computational spectroscopy was introduced, numerous computational spectrometers have emerged. While most of the work focuses on materials, optical structures, and devices, little attention is paid to the reconstruction algorithm, thus resulting in a common issue: the effectiveness of spectral reconstruction is limited under high-level noise originating from the data acquisition process. Here, we fabricate a computational spectrometer based on a quantum dot (QD) filter array and propose what we believe is a novel algorithm, TKVA (algorithm with Tikhonov and total variation regularization, and the alternating direction method of multipliers), to suppress the impact of noise on spectral recovery. Surprisingly, the new TKVA algorithm gives rise to another advantage, i.e., the spectral accuracy can be enhanced through interpolation of the precalibration data, providing a convenient solution for performance improvement. In addition, the accuracy of spectral recovery is also enhanced via the interpolation, highlighting its superiority in spectral reconstruction. As a result, the QD spectrometer using the TKVA algorithm shows supreme spectral recovery accuracy compared to the traditional algorithms for complex and broad spectra, a spectral accuracy as low as 0.1 nm, and a spectral resolution of 2 nm in the range of 400 to 800 nm. The new reconstruction algorithm can be applied in various computational spectrometers, facilitating the development of this kind of equipment.
研究领域:
光学工程
·
仪器科学与技术
·
信号处理
✓ 已验证
DOI
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2024
·
IF 9.4
Wenjing Yang,Chuanyin Zhou,Chongxian He,Youwen Yang,Wurikaixi Aiyiti,Liang Xu,Cijun Shuai
The demand for breast mesh with antitumor properties is critical in post-mastectomy breast reconstruction to prevent local tumor recurrence. Molybdenum-based oxide (MoO x ) exhibits enzyme-like activities by catalyzing endogenous hydrogen peroxide to produce reactive oxygen species for inducing tumor cell apoptosis. However, its catalytic activity is limited by insufficient active sites. Herein, a defect engineering strategy is proposed to create redox nanozymes with multiple enzymatic activities by incorporating Fe into MoO x (Fe-MoO v ). Fe-MoO v is subsequently integrated into polycaprolactone (PCL) to fabricate breast meshes for establishing an enzyme-catalyzed antitumor platform. The doping of Fe into MoO x formed numerous defect sites, including oxygen vacancies (OV) and Fe substitution sites, synergistically boosting the binding capacity and catalytic activity of Fe-MoO v . Density functional theory calculations demonstrated that the outstanding peroxidase-like catalytic activity of Fe-MoO v resulted from the synergy between OV and Fe sites. Additionally, OV contributes to the localized surface plasmon resonance effect, enhancing the photothermal capability of the PCL/Fe-MoO v mesh. Upon near-infrared laser exposure, the catalytic activity of the PCL/Fe-MoO v mesh is further improved, leading to increased generation of reactive oxygen species and enhanced antitumor efficacy, achieving 86.7% tumor cell mortality, a 264% enhancement compared to the PCL/MoO x mesh.
研究领域:
生物医学工程
·
材料科学
·
肿瘤学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
APPLIED CLAY SCIENCE
·
2017
·
IF 3.101
Lei Li,Guangxia Qi,Masami Fukushima,Bangda Wang,Hui Xu,Yi Wang
As one of layered double hydroxides (LDH) based heterogeneous catalysts , transition metal oxide pillared LDH composite has attracted considerable research interests as its excellent catalytic efficiency and thermal stability. However, during the preparation especially by reconstruction techniques, abundant interlayer regions of LDH are contaminated by some unexpected anion such as LDH affinitive CO 3 2 − , which will result in inaccessible interlayer active site. In this paper, magnetic nanoparticle Fe 3 O 4 pillared LDH composite was synthesized with exchangeable NO 3 − as the main interlayer anion by decomposition and reconstruction route. The anion exchange, decomposition and reconstruction processes were comprehensively investigated. XRD , TG-DTA-MS, SEM and other methods have been employed to characterize materials. The results show that anion exchange between ferric citrate anion and LDH follows Langmuir isotherm model and pseudo-second-order model. The decomposition and oxidation of anion exchange product (LDH-Fe(C 6 H 4 O 7 ) − ) can be complete at 550 °C for 20 min and crystal Fe 3 O 4 with nanostructure is formed between 500 and 600 °C. When calcined product (LDO-Fe 3 O 4 ) is reconstructed by rehydration in 0.015 M HNO 3 -30 wt% NaNO 3 mixture aqueous solution, CO 3 2 − contamination derived from CO 2 in air can be averted and lamellar structure is successfully recovered with Fe 3 O 4 and NO 3 − remaining in the interlayer gallery of LDH.
研究领域:
材料科学
·
催化化学
✓ 已验证
产品关联
DOI
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2025
·
IF 19
Jiawei Lin,Luhan Bao,Juan Wang,Tianyu Zhang,Wenguo Cui,Jing Ma
Auricular reconstruction is a key method for treating auricular deformities; however, the primary challenge is the creation of an integrated scaffold that accurately replicates the complex 3D structure of the auricle. In this study, the oxygen-demanding properties of Komagataeibacter xylinus are leveraged to guide microbial distribution in three dimensions by regulating the oxygen supply within the bioreactor, which enabled the production of an integrated artificial scaffold with a detailed auricular profile. These results indicate that this microbial manufacturing approach can be used to effectively design and fabricate auricular scaffolds of varying sizes and intricate structures. The scaffolds exhibited excellent mechanical properties, remained stable and non-degradable for over 50 days, and showed good biocompatibility with human chondrocytes and dermal fibroblasts. Subcutaneous implantation in Sprague-Dawley rats is successfully integrated into the surrounding tissues without causing rejection. This method of using microorganisms to fabricate integrated artificial auricular scaffolds holds significant promise and offers a viable alternative to clinical auricular reconstruction.
研究领域:
生物医学工程
·
组织工程
·
再生医学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）