CHINESE JOURNAL OF CATALYSIS
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2018
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IF 3.525
Jiaxin Gao,Xiaoyang Ou,Pei Xu,Minhua Zong,Wenyong Lou
We found a novel lipase gene in the Paenibacillus pasadenensis CS0611 strain. The lipase gene sequence was cloned into the pET-28a expression vector to construct a recombinant lipase protein containing 6 × His tags at the C- and N-termini, respectively. High-level expression of the lipase in E.coli BL21 (DE3) was obtained upon induction with IPTG at 20 °C. The recombinant lipase activity was approximately 1631-fold higher than the wild type. His-tagged recombinant lipase was purified rapidly and efficiently by using Ni-charged affinity chromatography with 63.5% recovery and a purification factor of 10.78. The purified lipase was stable in a broad range of temperatures and pH values, with the optimal temperature and pH being 50 °C and 7.0, respectively. Its activity was stimulated to different degrees in the presence of metal ions such as Ca 2+ , Mg 2+ , and some non-ionic surfactants. In addition, the purified lipase was activated by a series of water-miscible organic solvents such as some short carbon chain alcohols and was highly tolerant to some water-immiscible organic solvents.
研究领域:
生物化学
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分子生物学
·
酶工程
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产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2020
·
IF 10.652
Lei Huang,Min Wei,Shahid Zaman,Asad Ali,Bao Yu Xia
Integrating the merits of various catalytic materials into one novel hybrid is significant yet challenging in developing multifunctional electrode for overall water splitting. In this work, micro-platinum modification strategy is developed to construct the bifunctional Pt@Co 3 O 4 /Ni foam (NF) hybrid electrode for overall water splitting. The resultant Pt@Co 3 O 4 /NF electrode exhibits low overpotentials and favorable kinetics for both hydrogen (30 mV@10 mA cm −2 , 31 mV dec −1 ) and oxygen evolution (263 mV@10 mA cm −2 , 69 mV dec −1 ). Moreover, a voltage of ~ 1.53 V can drive a current density of 10 mA cm −2 for the two-electrode electrolyzer. The enhanced performance of Pt@Co 3 O 4 /NF hybrid could be attributed to the synergistic effect of micro-Pt modified Co 3 O 4 nanorod array with the optimized water cleavage and hydrogen recombination. This work not only reports an efficient hybrid electrode for overall water splitting but also offers valuable insights in designing multifunctional electrocatalysts for energy conversion technologies.
研究领域:
材料科学
·
能源化学
✓ 已验证
DOI
高影响因子
Natural Products and Bioprospecting
·
2024
·Vol. 14
·期 1
·1-16
·
IF 4.8
Yang Ya-Ya,Jing Shun-Li,Shao Jia-Li,Chen Ji-Xuan,Zhang Wei-Feng,Wan Si-Yuan,Shen Yu-Ping,Yang Huan,Yu Wei
Functionalized mesoporous materials have become a promising carrier for enzyme immobilization. In this study, Santa Barbara Amorphous 15 (SBA-15) was modified by N-aminoethyl- γ -aminopropyl trimethoxy (R). R-SBA-15 was employed to purify and immobilize recombinant β -glucosidase from Terrabacter ginsenosidimutans (BgpA) in one step for the first time. Optimum pH of the constructed R-SBA-15@BgpA were 7.0, and it has 20 ℃ higher optimal temperature than free enzyme. Relative activity of R-SBA-15@BgpA still retained > 70% at 42 ℃ after 8-h incubation. The investigation on organic reagent resistance revealed that the immobilized enzyme can maintain strong stability in 15% DMSO. In leaching test and evaluation of storage stability, only trace amount of protein was detected in buffer of the immobilized enzyme after storage at 4 ℃ for 33 days, and the immobilized BgpA still maintained > 50% relative activity. It also demonstrated good reusability, with 76.1% relative activity remaining after fourteen successive enzymatic hydrolyses of epimedin A to sagittatoside A. The newly proposed strategy is an effective approach for the purification and immobilization of BgpA concurrently. In addition, R-SBA-15@BgpA was demonstrated to have high efficiency and stability in this application, suggesting its great feasibility and potential to produce bioactive compounds such as secondary glycosides or aglycones from natural products. Graphical Abstract
研究领域:
生物化学
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酶工程
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材料科学
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产品关联
DOI
PubMed
高影响因子
IONICS
·
2022
·Vol. 28
·期 6
·2979-2989
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IF 2.961
Qi Jiqiu,Gu Huwei,Ruan Chenya,Zhu Lei,Meng Qingkun,Sui Yanwei,Feng Xiujuan,Wei Wenqing,Zhang Hao
MXene, an emerging 2D material, has aroused great concern in the field of supercapacitors owing to its charming properties. However, the relatively low specific capacitance of pristine MXene is unsatisfactory, which is caused by the restacking issue of MXene nanosheets. In this paper, a facile and cost-effective electrostatic self-assembly followed by hydrothermal method was adopted to prepare NiCo 2 S 4 decorated multilayer MXene nanosheets (MXene/NiCo 2 S 4 ). The introduced NiCo 2 S 4 effectively alleviates the self-restacking of MXene nanosheet and facilitates ion accessibility. Hence, the as-prepared MXene/NiCo 2 S 4 electrode displays a maximum specific capacitance of 1211.1 F g −1 at 1 A g −1 , surpassing the NiCo 2 S 4 electrode (938.4 F g −1 at 1 A g −1 ) and pure MXene electrode (84.2 F g −1 at 1 A g −1 ). The assembled asymmetrical supercapacitor utilizing hybrid MXene/NiCo 2 S 4 and RGO delivers a high energy density of 38.7 Wh kg −1 and high power density of 6137 W kg −1 . This work can provide a reliable reference for the synthesis of hybrid MXene/sulfide electrodes with ideal electrochemical performance.
研究领域:
材料科学
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电化学
✓ 已验证
DOI
高影响因子
Journal of the Mechanical Behavior of Biomedical Materials
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2020
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IF 3.372
Jingqiu Xu,Mingxing Li,Wenting Wang,Zhifang Wu,Chaoyang Wang,Xiaoting Jin,Ling Zhang,Wenxiang Jiang,Baiping Fu
Objectives The study investigated the effects of novel prime-&-rinse mode using MDP (10-methacryloyloxydecyl dihydrogenphosphate) and matrix metalloproteinase (MMPs) inhibitors on dentin microtensile bond strengths (MTBS) of self-etch adhesive, resin-dentin interface degradations, and activity of recombinant human (rh) MMP-8, -9. Materials and methods Eight experimental primers were prepared using 5% and 15% of MDP ethanol-aqueous (1:1) solution in combination with/without MMPs inhibitors (1%benzalkonium chloride (BAC), 1000 μm/mL polyvinylphosphonic acid (PVPA) and 15%proanthocyanidin (PA)). Ninety human mid-coronal dentin surfaces were applied with the experimental primers, water-sprayed and gently air-dried (prime-&-rinse mode), or not (control, self-etch mode). The specimens were bonded with self-etch adhesive (Clearfil S3 Bond) and composite resin (Clearfil Majesty). The resin-bonded specimens were prepared into multiple micro-beams for MTBS tests after 24 h and 1 yr of water storage. The resin-dentin interfaces were analyzed with SEM/TEM. The inhibitory effects of eight primers on rhMMP-8, 9 were determined. The data were analyzed using two-way ANOVA and LSD multiple comparisons tests. Results Compared with control, all the primers used in prime-&-rinse mode could significantly improve long-term dentin MTBS ( P < 0.05), while 5%MDP-BAC, 15%MDP and 15%MDP+MMPs inhibitors could significantly increase the short-term dentin MTBS ( P < 0.05). The SEM/TEM findings revealed that the resin-dentin interfaces were stable over time when the prime-&-rinse mode used. Eight primers possessed the high inhibitory ratio of rh MMP-8, 9. Conclusions The novel prime-&-rinse mode using 5%MDP-BAC, 15%MDP and 15%MDP+MMPs inhibitors could significantly increase the short- and long-term dentin MTBS of self-etch adhesive. This might be a supplement to contemporary dentin bonding strategies.
研究领域:
牙科学
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材料科学
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生物材料
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产品关联
DOI
PubMed
高影响因子
PHYTOPATHOLOGY
·
2014
·Vol. 104
·期 10
·
IF 2.746
Amara R. Dunn,Stephen R. Bruening,Niklaus J. Grünwald,Christine D. Smart
Populations of the vegetable pathogen Phytophthora capsici are often highly diverse, with limited gene flow between fields. To investigate the structure of a newly established, experimental population, an uninfested research field was inoculated with two single-zoospore isolates of P. capsici in September 2008. From 2009 through 2012, ≈50 isolates of P. capsici were collected from the field each year and genotyped using five microsatellite loci. The same two isolates were also crossed in the lab. High levels of diversity were detected in the research field, with 26 to 37 unique multilocus genotypes detected each year. Through 2012, genotypic diversity did not decline and no evidence of genetic drift was observed. However, during the 2011 and 2012 growing seasons, four new alleles not present in either parental isolate were observed in the field. Selfing (but not apomixis) was observed at low frequency among in vitro progeny. In addition, evidence for loss of heterozygosity was observed in half of the in vitro progeny. These results suggest that recombination, mutation, and loss of heterozygosity can affect the genetic structure observed in P. capsici populations.
研究领域:
植物病理学
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遗传学
✓ 已验证
DOI
高影响因子
Royal Society Open Science
·
2020
·Vol. 7
·期 9
·
IF 2.646
Siqi Liu,Xiaolin Xu,Yanshun Kang,Yingtian Xiao,Huan Liu
Ligninolytic enzymes, including laccase (Lac), manganese peroxidase (MnP) and lignin peroxidase (LiP), have attracted much attention in the degradation of contaminants. Genes of Lac (1827 bp), MnP (1134 bp) and LiP (1119 bp) were cloned from Aspergillus sp. TS-A, and the recombinant Lac (69 kDa), MnP (45 kDa) and LiP (35 kDa) were secretory expressed in Pichia pastoris GS115, with enzyme activities of 34, 135.12 and 103.13 U l−1, respectively. Dyes of different structures were treated via the recombinant ligninolytic enzymes under the optimal degradation conditions, and the result showed that the decolourization rate of Lac on Congo red (CR) in 5 s was 45.5%. Fourier-transform infrared spectroscopy, gas chromatography–mass spectrometry analysis and toxicity tests further proved that the ligninolytic enzymes could destroy the dyes, both those with one or more azo bonds, and the degradation products were non-toxic. Moreover, the combined ligninolytic enzymes could degrade CR more completely compared with the individual enzyme. Remarkably, besides azo dyes, ligninolytic enzymes could also degrade triphenylmethane and anthracene dyes. This suggests that ligninolytic enzymes from Aspergillus sp. TS-A have the potential for application in the treatment of contaminants.
研究领域:
环境科学
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生物技术
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微生物学
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产品关联
DOI
PubMed
高影响因子
npj Flexible Electronics
·
2022
·Vol. 6
·期 1
·1-8
·
IF 12.019
Li Xin,Zou Ruike,Liu Zhen,Mata Jitendra,Storer Ben,Chen Yu,Qi Weiheng,Zhou Zekun,Zhang Peng
In this work, a polymer and ionic liquid (IL) hybrid with superior thermoelectric performance is prepared via a system design of the chemical composition, molar ratio of the constituent molecules and manipulating the structure in solution and dried films. The solution-casted hybrid film, consisting of poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate) (PEDOT:PSS) and 1-ethyl-3-methylimidazolium tricyanomethanide (EMIM:TCM), shows the highest power factor of 175 μW m −1 K −2 in the polymer hybrid prepared by a post-treatment-free method. With a set of complementary structure characterization methods, it is found that EMIM:TCM can induce the structure reorganization of PEDOT:PSS in solution from a core-shell model to a rod-like model, during which PEDOT partially separates from PSS that eases the conductive network formation. In addition, the oxidation level of PEDOT:PSS is reduced by adding EMIM:TCM. Based on which, the PEDOT:PSS/IL hybrid shows the best performance in optimizing the conductivity (1163 S cm −1 ) and Seebeck coefficient (38.8 μV K −1 ) simultaneously.
研究领域:
材料科学
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物理化学
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产品关联
DOI
高影响因子
Nuclear Materials and Energy
·
2021
·
IF 2.32
S. Krat,Yu. Gasparyan,Ya. Vasina,A. Prishvitsyn,G.De Temmerman,A. Pisarev
Available literature data for beryllium content in D-Be co-deposited layers was modeled using newly developed model. It was shown that the model adequately describes the experimental results. An empirical scaling for the available trap concentrations as a function of deuteron energy was introduced in order to improve the agreement between the experimental data and model predictions. The limited recombination rate describes the co-deposition better than the unlimited one. Assuming two types of traps in the film with detrapping energies of 0.8–0.9 and 1.2–1.3 eV and trap concentrations of 10 at. % and 1 at. %, respectively, and the deuterium implantation energy of 15.6 eV, the average relative deviation between experimental and predicted D/Be values of 1.9 was observed, which is better than the empirical D/Be scalings.
研究领域:
核物理学
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材料科学
✓ 已验证
DOI
高影响因子
JOURNAL OF ALLOYS AND COMPOUNDS
·
2021
·
IF 5.316
Jinhua Wang,Qiong Zhu,Yunwen Liao,Hongquan Fu,Jinming Chang,Yuling Zhang,Taotao Kan,Hejun Gao,Wenchao Huang
Nano-CuO have been grown on the surface of CdS nanorods by a simple solution-phase reaction, which greatly enhanced the hydrogen evolution ability of CdS. The physical chemistry properties of CuO/CdS nanocomposites were investigated by XRD, SEM, TEM, BET, XPS and electrochemical methods. The morphology and crystal structure of CdS were not influenced by the loading of Nano-CuO. And it was helpful to delay the time of electron recombination to the hole, and greatly improved the hydrogen evolution performance of CdS. When the loading of Nano-CuO was 1 wt%, the photocatalytic performance of CdS increases from 105 μmol g −1 h −1 to 3317.5 μmol g −1 h −1 . After several cycles, the structure of CuO/CdS nanocomposites was not changed, and Nano-CuO could effectively prevent the photo-corrosion defects of CdS. This work provides a new way to improve the hydrogen evolution performance of CdS by growing nano-oxide on CdS surface.
研究领域:
材料科学
·
能源化学
✓ 已验证
DOI
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2025
·
IF 9.7
Huihua Niu,Jiongliang Yuan,Yuning Su,Lingjia Ma,Shuaishuai Xiao,Donglin Song,Xinlu Xiong,Qiang Ren,Guangzhao Wang
High-entropy alloy (HEA) catalysts have great potential for hydrogen production in electrocatalytic water splitting, which is superior to conventional Pt/C catalysts. In this work, the noble-metal-free HEA CoNiCuMoZn was fabricated by pulsed electrodeposition, and its structural reorganization was carried out by cyclic voltammetry. The reconstituted HEA, R-CoNiCuMoZn, was changed to spherical particles with many wrinkles on the surface. Co, Ni, Mo, and Zn in R-CoNiCuMoZn exhibited an elevated electron cloud density, thus promoting the generation of absorbed hydrogen (H ads ), which benefited hydrogen evolution reaction (HER). The electron paramagnetic resonance (EPR) results showed that Mo vacancies increased after structural reorganization, which was the main reason for the enhanced hydrogen evolution performance. The addition of the metal Zn with weak H ads binding affinity accelerates the Heyrovsky step. The R-CoNiCuMoZn catalyst achieved an ultra-low overpotential of only 43.0 ± 0.3 mV in 1.0 M KOH solution at the current density of 400 mA cm −2 , and it exhibited a Tafel slope of 21.20 mV dec −1 . After a stability test of 50 h, there was no significant decay in current density. Density functional theory (DFT) calculation reveals that Mo vacancies reduce the dissociation energy barrier, and metal Zn reduces the Helovsky step activation energy. This study provides a new approach for developing high-efficiency HER electrocatalysts.
研究领域:
材料科学
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电化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SOLAR ENERGY MATERIALS AND SOLAR CELLS
·
2025
·
IF 6.3
Wenbo Cao,Chong Chen,Mingtai Wang
CuInS 2 is a promising light-harvesting material for solar cells due to its long-term stability, suitable bandgap and favorable optical absorption coefficient. Here, solar cells with In-rich CuInS 2 light-active layer are fabricated by increasing the feed ratio in precursor solution. The CuAu phases within chalcopyrite CuInS 2 films are inhibited with increased In molar ratio, leading to a better quality of CuInS 2 films as well as reduction in charge recombination centers in solar cells. Moreover, the band alignment of CuInS 2 /Spiro-OMeTAD interface is adjusted by increased In molar ratio, and the charge carriers are effectively separated at the CuInS 2 /Spiro-OMeTAD interface due to the lower valence band level of In-rich CuInS 2 films. These improvements result in a planar CuInS 2 solar cell with an efficiency of 7.66% and an open-circuit voltage reached 0.85 V. The In-rich CuInS 2 devices exhibit the lower voltage loss, which enhances the potential for high-efficiency solar cells.
研究领域:
材料科学
·
光伏技术
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）