JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
·
2024
·
IF 2.8
Jun Zhang,Ze-hua Liu,Ke-meng Zhao,Zhi Dang,Yun Liu,Yu Liu
This work for the first time reported the complete transformation of 17β-estradiol (E2) to estrone (E1) by unknown wild-type enzyme present in the widely used commercial arylsulfatase derived from Helix pomatia . It was found that acetate could effectively inhibit the unknown enzyme with a half inhibitory concentration (IC 50 ) of 140.9 μM, while phosphate and citrate showed no inhibition. Since the buffer solutions with phosphate and citrate have been used in the enzymatic hydrolysis of natural estrogen conjugates for decades, the transformation of E2 to E1 likely occurred during such procedure, inevitably leading to overestimated E1, but underestimated E2. It was further suggested that acetate should be used to prevent this undesirable transformation during the enzymatic hydrolysis of natural estrogen conjugates.
研究领域:
生物化学
·
环境科学
·
医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2015
·Vol. 20
·期 4
·5680-5697
·
IF 2.416
Shanshan Zhang,Xiaoqian Liu,Lihua Yan,Qiwei Zhang,Jingjing Zhu,Na Huang,Zhimin Wang
Armillaria melleais a traditional Chinese medicinal and edible mushroom. Many cultured products ofA. melleahave been used to develop commercial medicines in recent years. The chemical composition and activities of the major bioactive chemical components—polysaccharides—may be different because of differences in the raw materials used. Four polysaccharides (SP, CMP, CFBP and CFMP) were obtained from wild sporophores and cultured products (including mycelia, fermentation broth and fermentation mixture) ofA. mellea. Their yields, carbohydrate contents, monosaccharide compositions, FT-IR spectra, NMR spectroscopy and antioxidant activities were investigated. All of the polysaccharides were composed of xylose, glucose and galactose without protein. Glucose was the dominant monosaccharide in SP, CMP and CFMP, whereas galactose was the dominant monosaccharide in CFBP. SP and CMP showed higher scavenging DPPH•and ABTS•+activities and reducing power among four polysaccharides. The carbohydrate content and corresponding glucose percentage were positive influences on the antioxidant activities, whereas the corresponding xylose and galactose percentage were negative influences.A. melleapolysaccharides are potential natural antioxidants. Polysaccharides from cultured products, especially mycelia, are good substitutes for SP and are also potential sources for both dietary supplements and food industries.Keywords:Armillaria mellea;polysaccharides;wild sporophores;cultured products;chemical composition;antioxidant activity
研究领域:
微生物学
·
食品科学
·
药理学
✓ 已验证
DOI
PubMed
高影响因子
MOLECULES
·
2025
·Vol. 30
·期 3
·468
·
IF 4.6
Fukang Kong,Yannan Kou,Xu Zhang,Yue Tian,Bin Yang,Weihao Wang
The dried rhizome ofNotopterygium incisum(NI) from theUmbelliferaefamily, genuinely produced in Sichuan, China, is a classic traditional Chinese medicinal herb for treating wind-dampness arthralgia. Due to scarce natural resources, wild NI is gradually being replaced by cultivated types. However, knowledge is limited regarding the differences in chemical composition and pharmacological effects between wild and cultivated NI and between Sichuan-grown and other-region-grown NI. In this study, a plant metabolomics strategy, based on GC–MS and UHPLC-Orbitrap MS, was employed to compare metabolic profiles between wild and cultivated NI and between cultivated NI from Sichuan and cultivated NI from Gansu and Qinghai. In total, 195 metabolites were identified, and the biosynthetic pathways of coumarins and phenolic acids, which were the most abundant secondary metabolites in NI, were summarized. Additionally, seven key metabolic intermediates were uncovered, revealing the reasons for the differences in metabolic profiles between wild and cultivated NI. The anti-inflammatory effect of wild and cultivated NI was verified by inflammatory gene expression and neutrophil count using a zebrafish yolk sac inflammation model. Overall, this study presents information on the types and synthesis of pharmacodynamic substances in NI and provides a basis for its cultivation and applications.
研究领域:
药用植物学
·
代谢组学
·
中药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Bioscience of Microbiota Food and Health
·
2020
·
IF 1.906
Wenyan HUO,Peng QI,Langjun CUI,Liguang ZHANG,Lu DAI,Yu LIU,Suying HU,Zhengping FENG,Ting QIAO,Junzhi LI
Polysaccharides from morels possess many characteristics beneficial to health, such as anti-tumor and immunomodulatory activities. The gut microbiota plays a critical role in the modulation of immune function. However, the impact of morel polysaccharides on the gut microbiota has not yet been explored. In this study, a high-throughput pyrosequencing technique was used to investigate the effects of MP, a new heteropolysaccharide extracted from wild morels, on the diversity and composition of microbiota along the intestine in mice, as well as the production of short-chain fatty acids (SCFAs). The results showed that MP treatment increased the number of operational taxonomic unit (OTUs) and diversity along the intestine, especially in the small intestine. MP treatment induced a significant decrease in the number of Firmicutes and a significant increase in the number of Bacteroidetes in the small intestine microbiota. It was also observed that the relative abundance of SCFA-producing bacteria, especially Lachnospiraceae, was increased in both the cecum and colon of MP-treated mice. Moreover, MP promoted the production of SCFAs in mice. These results provide a foundation for further understanding the health benefits conferred by morel polysaccharides.
研究领域:
微生物学
·
营养学
·
免疫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
·
2021
·
IF 3.205
YueTao Liu,ZiYing Wang,XueMei Qin
Astragali Radix (AR) has been widely used as bulk medicines in China. Currently, there are mainly two types in the market, wild-simulated AR (WAR) and transplanted AR (TAR). The present work was designed to compare their effects in treatment of blood deficiency with its compatibility with Angelicae Sinensis Radix, a classical formula Danggui Buxue decoction (DBD). UHPLC-Q-Exactive-MS-based serum metabolomics was firstly applied to compare their metabolic regulations in blood deficiency mice. Metabolic results showed that 17 metabolites were closely related to blood deficiency. WAR-DBD and TAR-DBD significantly improved 13 and 8 metabolites among them, respectively. Further, fourteen genes were selected as promising targets for treating blood deficiency by searching GeneCards and CTD database. The results of molecular docking also showed that eight components of AR and three serum metabolites had strongest correlations with blood deficiency targets. The results further revealed that WAR-DBD was better than TAR-DBD against blood deficiency. The method provided a new way to compare the hematopoietic differences of AR of different cultivation methods. In addition, molecular docking plays an irreplaceable role in explaining the molecular mechanism of biological processes .
研究领域:
中药学
·
代谢组学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2020
·
IF 5.162
Linlin Hou,Yaliang Huang,Weilin Hou,Yurou Yan,Jinlin Liu,Ning Xia
Sensitive and accurate quantification of wild-type p53 protein is of great importance for biological research and clinical diagnosis. Herein, a modification-free amperometric biosensor was proposed for sensitive detection of wild-type p53 protein by the signal amplification of silver nanoparticles (AgNPs) networks formed in situ on electrode surface. Double-stranded DNA (dsDNA) probe containing two consensus sites was immobilized on gold electrode surface to capture wild-type p53 protein. The cysteine thiol and amine groups on the exterior of the protein allowed for the attachment of bare AgNPs through the Ag S or Ag N interactions. Meanwhile, benzene-1,4-dithiol (BDT) molecules in solution triggered the assembly of more AgNPs on electrode surface through the Ag S interactions, thus leading to the in situ formation of AgNPs networks for signal amplification. The target at the concentration as low as 0.1 pM can be readily determined. This method was further applied to determine wild-type p53 protein in spiked human serum and cell lysates with satisfactory results. Moreover, the biosensor is regenerative and does not require the modification of AgNPs with recognition element for signal readout. The modification-free strategy can potentially be applied to develop novel biosensors for detection of other biological macromolecules.
研究领域:
生物传感器
·
癌症诊断
·
分析化学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Sustainable Chemistry & Engineering
·
2024
·
IF 7.1
Jiahui Gong,Mengfei Tian,Ying Liu,Yuting Zhang,Junhui Xing,Qi Liang,Xiaofei Xie,Chunying Li,Chunjian Zhao
In this work, a dual-responsive ionic liquid (DR-IL), namely, [C4mim][FeCl4] was developed for simultaneously extracting and separating hydrophilic phytochemicals, including epicatechin (EC), dihydroquercetin (DQ), ferulic acid (FA), and hydrophobic phytochemicals, such as emodin (ED), chrysophanol (CP), and physcion (PC), from roots of wild Rumex acetosa L. The [C4mim][FeCl4] demonstrates considerable potential for extracting and separating target phytochemicals through temperature adjustment and accelerated phase separation facilitated by a neodymium magnet. Meanwhile, a high recovery yield is obtained by adding a magnetic field. The overall extraction yield of target phytochemicals achieved by [C4mim][FeCl4] reaches 16.12 mg/g under the optimal extraction conditions, representing a 1.53–2.39-fold increase compared to conventional solvents. Furthermore, the molecular simulation and FT-IR analysis reveal that the synergistic effect of van der Waals forces and hydrogen bonds significantly contributed to the higher extraction yields of target phytochemicals.
研究领域:
植物化学
·
分析化学
·
药用植物研究
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2024
·
IF 4
Zhixin Fei,Hang Zhang,Qing Song,Mingyue Sun,Chengxi Li,Min Liu
Ubiquitous perchlorate contamination has become an important food safety issue related to human health, but contamination characteristics and health risks of perchlorate in wild edible mushrooms remain unclear. In this study, a method based on dispersive solid phase extraction (d-SPE) clean-up procedure combined with liquid chromatography-tandem mass spectrometry (LC-MS/MS) was established for the rapid analysis of perchlorate in wild edible mushrooms. Sample pretreatment was achieved by acetonitrile extraction followed by clean-up with florisil and graphitized carbon black (GCB). The proposed method presented satisfactory linearity, accuracy, precision, negligible matrix effects, and low limit of detection (LOD) (0.15 µg/kg) and limit of quantification (LOQ) (0.5 µg/kg). The recoveries at four levels (0.5, 3, 30 and 300 µg/kg) were 93.1–108.7% with relative standard deviation ranging from 2.9% to 18.2% for mushroom samples. The validated methodology was successfully applied to detect 109 wild edible mushroom samples belonging to 8 species collected from Yunnan, China, and 95.41% of the samples were found to have detectable perchlorate contamination. Concentrations of perchlorate ranged from below LOD to 29.81 μg/kg, with a mean value of 4.11 μg/kg. The risk assessment results for perchlorate in wild edible mushrooms showed no health risks to consumers.
研究领域:
食品科学
·
环境科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
Food Analytical Methods
·
2019
·Vol. 12
·期 12
·2908-2919
·
IF 2.413
Xie Zenghui,Zhang Lanyun,Chen Yanli,Hu Xujia
In this study, novel magnetic molecularly imprinted polymers (MMIPs) with specific adsorption for glutathione were prepared. Based on MMIPs as adsorbents for magnetic solid-phase extraction, we combined magnetic solid-phase extraction with high-performance liquid chromatography (MPSE-HPLC) for the separation, enrichment, and detection of glutathione in nine kinds of wild edible bolete extracts. MMIPs were characterized by Fourier transform infrared spectrometer (FT-IR), X-ray diffraction (XRD), transmission electron microscopy (TEM), and vibrating sample magnetometer (VSM). In addition, the properties of MMIPs were studied by adsorption isotherm, adsorption kinetic, and selective adsorption experiments. The results showed that MMIPs had high selectivity for glutathione ( K d = 245.33 mL/g), and adsorption time and saturated adsorption capacity were 30 min and 17.88 μmol/g, respectively. Under the optimized conditions, there was a good linear relationship in the concentration range of 100~3000 μg/L, the correlation coefficient R 2 = 0.9997, and the limit of detection (LOD) and the limit of quantitation (LOQ) that were 24.828 μg/L and 82.760 μg/L, respectively. The spiked recovery and RSD were 93.28~103.03% and 2.77~5.11, respectively. We successfully combined MSPE-HPLC for the detection of glutathione in nine kinds of wild edible bolete extracts. It was found that the content of glutathione in Leccinum extremiorientale (L. Vass.) Sing. was the highest (19.800 mg/g) and that in Boletus aereus was the lowest (6.791 mg/g).
研究领域:
食品科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
Plants-Basel
·
2025
·Vol. 14
·期 24
·3718
·
IF 4.1
Xinxin Li,Ziting Zhu,Jing Wu,Nianjun Yu,Huiqun Xie,Lan Han,Daiyin Peng
Dendrobium huoshanense , a rare and endangered medicinal orchid species endemic to Huoshan County, Anhui Province, faces a severe limit of wild resources. Its medicinal efficacy derives from unique chemical constituents, which vary significantly across cultivation modes. We systematically compared sensory properties and nutritional constituents (polysaccharides, flavonoids, alkaloids, bibenzyls, minerals, and free amino acids) between D. huoshanense in three cultivation modes—greenhouse cultivation, understory gravel cultivation, and wild-simulated cultivation. Additionally, the gastroprotective effects of wild-simulated cultivated D. huoshanense on gastric mucosal epithelial cells were evaluated. Results demonstrated that wild-simulated cultivation yielded significantly higher levels of nutritional constituents compared to gravel and greenhouse cultivation. Furthermore, wild-simulated cultivated D. huoshanense exhibited notable protective effects against gastric mucosal epithelial cell damage. This study not only elucidates the influence of cultivation modes on the chemical profile of D. huoshanense but also provides scientific evidence supporting its gastric protective properties, offering a foundation for its sustainable utilization and quality-oriented cultivation.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RESPIRATORY RESEARCH
·
2024
·Vol. 25
·期 1
·1-16
·
IF 4.7
Wang Han,Du Xiaohui,Liu Wenwen,Zhang Congcong,Li Ying,Hou Jingwen,Yu Yi,Li Guiru,Wang Qi
BackgroundEpidermal growth factor receptor (EGFR) tyrosine kinase inhibitors (TKIs) have revolutionized the treatment of lung cancer patients with mutated EGFR. However, the efficacy of EGFR-TKIs in wild-type EGFR tumors has been shown to be marginal. Methods that can sensitize EGFR-TKIs to EGFR wild-type NSCLC remain rare. Hence, we determined whether combination treatment can maximize the therapeutic efficacy of EGFR-TKIs.MethodsWe established a focused drug screening system to investigate candidates for overcoming the intrinsic resistance of wild-type EGFR NSCLC to EGFR-TKIs. Molecular docking assays and western blotting were used to identify the binding mode and blocking effect of the candidate compounds. Proliferation assays, analyses of drug interactions, colony formation assays, flow cytometry and nude mice xenograft models were used to determine the effects and investigate the molecular mechanism of the combination treatment.ResultsBetulinic acid (BA) is effective at targeting EGFR and synergizes with EGFR-TKIs (gefitinib and osimertinib) preferentially against wild-type EGFR. BA showed inhibitory activity due to its interaction with the ATP-binding pocket of EGFR and dramatically enhanced the suppressive effects of EGFR-TKIs by blocking EGFR and modulating the EGFR-ATK-mTOR axis. Mechanistic studies revealed that the combination strategy activated EGFR-induced autophagic cell death and that the EGFR-AKT-mTOR signaling pathway was essential for completing autophagy and cell cycle arrest. Activation of the mTOR pathway or blockade of autophagy by specific chemical agents markedly attenuated the effect of cell cycle arrest. In vivo administration of the combination treatment caused marked tumor regression in the A549 xenografts.ConclusionsBA is a potential wild-type EGFR inhibitor that plays a critical role in sensitizing EGFR-TKI activity. BA combined with an EGFR-TKI effectively suppressed the proliferation and survival of intrinsically resistant lung cancer cells via the inhibition of EGFR as well as the induction of autophagy-related cell death, indicating that BA combined with an EGFR-TKI may be a potential therapeutic strategy for overcoming the primary resistance of wild-type EGFR-positive lung cancers.Graphical abstract
研究领域:
肿瘤学
·
药理学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
MEDICAL ONCOLOGY
·
2016
·Vol. 33
·期 2
·1-9
·
IF 2.486
Li Yang-ling,Pan Yi-ni,Wu Wen-jue,Mao Shi-ying,Sun Jiao,Zhao Yi-ming,Dong Jing-yin,Zhang Da-yong,Pan Jian-ping,Zhang Chong,Lin Neng-ming
Erlotinib is effective in NSCLC patients with known drug-sensitizing EGFR mutations, but its clinical efficacy in patients with wild-type EGFR or acquired resistance to erlotinib remains modest. Evodiamine is a chemical extracted from the Evodia rutaecarpa (Juss.) Benth, we showed that evodiamine could induce anti-proliferation and apoptosis in four wild-type EGFR NSCLC cell lines, and combining evodiamine with erlotinib might successfully inhibit cell proliferation and survival in wild-type EGFR NSCLC cells, characterized as erlotinib-resistant. In addition, evodiamine plus erlotinib significantly increased the apoptotic rate of NSCLC cells, as compared to single agent treatment alone. Further investigation of the mechanism underlying these effects revealed that evodiamine plus erlotinib might downregulate Mcl-1 expression through the mTOR/S6K1 control of its translation. Thus, our study has revealed evodiamine as a pertinent sensitizer to erlotinib and the strategy of combining erlotinib with evodiamine appears to be an attractive option for reversing resistance to erlotinib.
研究领域:
肿瘤学
·
药理学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）