LUMINESCENCE
·
2020
·Vol. 35
·期 7
·989-997
·
IF 1.855
Zhu Han,Li He,Shuang Pan,Hui Liu,Xiaoli Hu
A novel and sensitive fluorescence analysis platform was constructed for the detection of chlorogenic acid (CGA) using carbon dots (C-dots) with prominent sensitivity and selectivity. Excitation-dependent emission fluorescence C-dots were fabricated using citric acid and l -histidine as precursors through an efficient one-step hydrothermal treatment. The maximum excitation and emission wavelength of the as-synthesized C-dots were 340 nm and 414 nm, respectively. Moreover, the as-prepared C-dots displayed excellent water solubility and good photostability. The fluorescence quantum yield of the as-prepared C-dots was measured to be about 22% using quinine sulfate as the reference. Furthermore, the obtained C-dots were applied to the detection of CGA accompanied with a wide linear range from 1.53 μmol L −1 to 80.0 μmol L −1 as well as a limit detection of 0.46 μmol L −1 . More importantly, the proposed fluorescence method was successfully used to analyse CGA in coffee and honeysuckle.
研究领域:
分析化学
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食品科学
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产品关联
DOI
PubMed
高影响因子
Journal of Environmental Chemical Engineering
·
2025
·
IF 7.2
Weiqian Cai,Youqun Wang,Linshan Xiong,Yingjie Zheng,Zhibin Zhang,Xiaohong Cao,Yunhai Liu
Cuprous oxide (Cu 2 O), a promising semiconductor material, has been less employed in the photocatalytic removal of uranium. In this work, silver and gold nanoparticles were introduced on the surface of Cu 2 O. HR-TEM, XRD and XPS were applied in characterized the physic-chemical properties of composite. In comparison to Cu 2 O, Au modified Cu 2 O (Au/Cu 2 O-N) demonstrated a significantly enhanced uranium removal rate, reaching 100% within 120 minutes. This represents a 2-fold increase in the uranium removal rate compared to pure Cu 2 O-N. The reason con be interpreted including the red-shift of the absorption band from 600nm-800nm, the formation of Schottky junction between Au and Cu 2 O, the electron traps constructed by Au itself. Consequently, the rate of photogenerated electron-hole complexation is significantly diminished. Free trapping and EPR prove that reactive radicals are O 2 - . This has led to the development of a novel approach, namely the synergistic strategy of noble metal loading and nitrogen-doped cuprous oxide, which has the potential to facilitate the visible light removal of U(VI) from toxic wastewater.
研究领域:
材料科学
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环境科学
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DOI
高影响因子
Processes
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2022
·Vol. 11
·期 1
·110
·
IF 3.352
Mingsheng Luo,Xinyue Liu,Zhijun Zhao,Fengli Wang,Changke Shao
Using honeysuckle as raw material, chlorogenic acid (CGA) was extracted with different alcohols. Based on the single-factor experiment design, the relationship between each parameter and the response value was explored by Box–Behnken method to optimize the process conditions. Best extraction results were obtained under the conditions of solid-to-liquid ratio of 1:20, the ultrasonic time of 40 min, the ultrasonic vibrator power of 240 w, and the CGA extraction rate of 2.98%. The experimental data show that the extraction rate of CGA is related to the length of the alcohol carbon chain and the number of hydroxyl groups in the extractant. The results from this work can provide technical basis for the safe and efficient production of CGA from honeysuckle.
研究领域:
植物化学
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药物化学
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食品科学
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DOI
高影响因子
CHEMICAL & PHARMACEUTICAL BULLETIN
·
2018
·Vol. 66
·期 6
·608-611
·
IF 1.258
Jiaxin Li,Yujie Wang,Jian Xue,Pengsi Wang,Shangmei Shi
To investigate the dietary exposure risk of flonicamid application on Lonicerae Japonicae Flos and the effect of flonicamid on constituents of Lonicerae Japonicae Flos, field experiments were conducted in Fengqiu, Henan province, and flonicamid residue in samples collected was detected by gas chromatography equipped with electron capture detector (GC-ECD). And chlorogenic acid and luteoloside were determined by HPLC. Dietary exposure risk assessment was conducted through comparing the estimated daily intake (EDI) which was calculated by using the consumed residual level along with the acceptable daily intake (ADI). The effect of flonicamid on chlorogenic acid and luteoloside were obtained by ANOVA statistical analysis and least significant difference (LSD)-t test. The results showed that the terminal-residue contents of flonicamid were under 1.6 mg kg−1. And risk quotient ranged from 0.0011 to 0.0028, indicating the long-term exposure to flonicamid residual through consumption of Lonicerae Japonicae Flos in consumers was relatively low. Flonicamid could suppress the generation of luteoloside, so it was not advised to be used in L. japonica flowering phase. The study aims at providing the useful suggestion on the reasonable flonicamid usage and the reference for the establishment of maximum residue limits (MRLs) of flonicamid in Lonicerae Japonicae Flos.
研究领域:
植物学
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药理学
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食品安全
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Wenting Gao,Bin Mu,Fangfang Yang,Yalong Li,Xiaomei Wang,Aiqin Wang
In order to fulfill people's requirements for food quality and safety, it is a promising strategy to develop intelligent biodegradable food packaging materials. Herein, honeysuckle extracts/attapulgite/chitosan composite films containing natural carbon dots were fabricated for intelligent food packaging. Different characterization techniques were employed to study the obtained composite films, while the physicochemical properties, optical properties, antibacterial and antioxidant activities of composite films were determined. The obtained composite films presented good mechanical, antibacterial and antioxidant properties, and the antibacterial ratios of composite films against Escherichia coli and Staphylococcus aureus were 99.27 ± 0.18 % and 98.85 ± 0.65 %, respectively. When the added amount of honeysuckle extracts/attapulgite nanocomposites was 4.76 %, the tensile strength and elongation at break of composite films reached 24.9 ± 2.35 MPa and 64.8 ± 2.11 %, respectively, which were obviously higher than that of pure chitosan film. Furthermore, the composite films exhibited excellent UV shielding and blue fluorescence properties, as well as pH-sensitivity due to the presence of caffeoquinic acid-based natural carbon dots derived from honeysuckle extract. Therefore, the composite films indicated a potential application for intelligent food packaging.
研究领域:
食品科学
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材料科学
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生物工程
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
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2018
·
IF 2.441
Xiao-Hui Yao,Jian-Yi Xu,Jing-Yi Hao,Yi Wan,Tao Chen,Dong-Yang Zhang,Long Li
Flos Lonicerae was an important Chinese medicine. In this research, a microwave assisted extraction method was applied for the extraction of chlorogenic acid from Flos Lonicerae . The operating conditions were optimized using a Box–Behnken design test. Under the optimal conditions, the extraction yield of chlorogenic acid reached 32.52 ± 1.31 mg/g. Next, a direct analysis in real time mass spectrometry (DART-MS) method was utilized to quantify of chlorogenic acid in Flos Lonicerae extracts. The primary parameters were optimized to obtain maximum signal intensity. In the detection process of the actual samples, the results obtained by DART–MS are consistent with those obtained by HPLC method with short detection time and acceptable repeatability and precision (<15%). In addition, the DART–MS/MS method has several advantages, such as speed, low cost and simplicity. Therefore, the DART-MS method is an efficient method that can be applied in the quantification of chlorogenic acid in Flos Lonicerae.
研究领域:
中药学
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分析化学
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DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
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2021
·
IF 4.821
Xiupei Yang,Jing Yang,Maoxue Zhang,Ya Wang,Baowen Zhang,Xiaolin Mei
Tiopronin (TPN) protected gold-silver bimetallic nanoclusters (TPN-AuAg NCs) were synthesized in an aqueous medium by a one-pot method. The synthesized TPN-AuAg NCs are spherical with an average diameter of 1.75 ± 0.5 nm and can emit bright fluorescence in the red spectral range ( E m = 670 nm). Based on the “on-off-on” process of the nanoclusters' fluorescence, a fluorescent probe for sequential detection of Fe 3+ and ascorbic acid (AA) was proposed. After adding Fe 3+ ions into the TPN-AuAg NCs solution, a composite Fe 3+ @TPN-AuAg NCs could be formed. Due to the internal filtering effect (IFE) between Fe 3+ and TPN-AuAg NCs, the fluorescence of the nanoclusters was quenched. Subsequently, when AA was continuously added to the fluorescence quenching system, based on the reduction effect of AA on Fe 3+ in the complex, the fluorescence of Fe 3+ @TPN-AuAg NCs could be sensitively turned on. The detection limits of the fluorescence titration measurements calculated using the equation 3σ/k, were 150 nM (Fe 3+ ) and 60 nM (AA), respectively. The proposed method has been successfully applied to detect Fe 3+ and AA in human serum samples.
研究领域:
分析化学
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生物医学工程
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纳米科技
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产品关联
DOI
高影响因子
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
·
2014
·
IF 1.862
Ping Jiang,Yu-chen Sheng,Yu-hao Chen,Li-li Ji,Zheng-tao Wang
This study aims to observe the protective action of Flos Lonicerae (FL) aqueous extract against acetaminophen (AP)-induced liver injury and its mechanism. Results show that FL decreases AP-increased serum alanine/aspartate transaminases (ALT/AST) activity, as well as total bilirubin (TB) amount, in mice. Histological evaluation of the liver further confirms the protection of FL against AP-induced hepatotoxicity. TdT-mediated biotin-dUTP nick-end labeling (TUNEL) assay shows that FL reduces AP-increased apoptotic cells. Furthermore, AP-decreased liver glutamate-cysteine ligase (GCL) enzymatic activity and glutathione (GSH) amount are both reversed by FL because of the increased expression of the catalytic subunit of GCL (GCLC) protein. The amount of chlorogenic acid (CGA), caffeic acid, and luteolin, the main active compounds in FL, is detected by high-performance liquid chromatography (HPLC). In addition, cell viability assay demonstrates that polyphenols in FL, such as CGA, caffeic acid, as well as isochlorogenic acids A, B, and C, can reverse AP-induced cytotoxicity. In conclusion, FL can prevent AP-induced liver injury by inhibiting apoptosis. The cellular antioxidant enzyme GCL is also involved in such protection. Polyphenols may be the main active hepato-protective ingredients in FL.
研究领域:
药理学
·
肝脏病学
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DOI
PubMed
高影响因子
AMERICAN JOURNAL OF CHINESE MEDICINE
·
2025
·Vol. 53
·期 02
·543-566
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IF 5.5
Jia-Yuan He,Fu-Lan Xiao,Qin-Yue Zheng,Chang-Hong Wang,Yi-Yue Tang,Jun-Xuan Fu,Jia-Yi Huang,Lian-Di Zhou,Qi-Hui Zhang
The intestinal absorption of active herbal constituents plays an important role in the biomedical efficacy of Traditional Chinese Medicine (TCM) formulations after oral administration. TCM compounds with low oral bioavailability can reach the distal intestine and then interact with intestinal flora, influencing the botanical pharmacological effects. In this study, in vitro digestion and an ex vivo Ussing chamber model were utilized to evaluate the intestinal absorption behavior of Forsythiae Fructus - Lonicerae Japonicae Flos -containing Yinqiao Jiedu Granule (YQJDG). It was found that the jejunum exhibited active absorption effects for some components of the formula, while the oral bioavailability of other herbal ingredients was low. Through further research using a combined UPLC-MS/MS and 16S rDNA sequencing technique, we studied the existence of the reciprocal interactions between YQJDG and gut microbiome. The in vitro fecal fermentation results showed that YQJDG significantly impacted the microbial community composition. The YQJDG markedly increased the abundance of beneficial microorganisms, such as Parabacteroides distasonis and Streptococcus gallolyticus subsp. Macedonicus, and suppressed the abundance of conditional pathogens including Prevotella steorerea, Haemophilus parainfluenzae , and Bacteroides . These effects may potentially contribute to the body’s immune functions and anti-inflammatory capacities. UPLC-MS/MS analysis suggested that the fecal microbiota chemically transformed constituents with low bioavailability to more readily absorbed potentially active metabolites. These findings provided valuable insights into the absorption characteristics of YQJDG and its interaction with the gut microbiome, further facilitating our understanding of precise pharmacological mechanisms of action of this Chinese herbal formulation.
研究领域:
中药学
·
微生物学
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药理学
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产品关联
DOI
PubMed
高影响因子
CERAMICS INTERNATIONAL
·
2024
·
IF 5.1
Wei Gao,Jinglei Zhang,Lin Shao,Jiaxi Li,Peng Ding,Qingyan Han,Xuewen Yan,Chenyun Zhang,Jun Dong
Enhancing upconversion luminescence in rare-earth materials through the plasmonic effects of precious metals has emerged as a highly effective strategy. In this study, we explore three distinct self-assembly techniques to fabricate alloy nano-film substrates using silver-coated gold (Au@Ag) core-shell nanoparticles, each with unique structural attributes. To enhance the upconversion luminescence of Er 3+ ions, we combine these substrates with NaYF 4 :Yb 3+ /Er 3+ nanoparticles, creating NaYF 4 :Yb 3+ /Er 3+ /Au@Ag composite nanostructures. The upconversion emission of Er 3+ ions is observably boosted when directly combined with Au@Ag nano-films of various structures under a 980 nm laser excitation. Surprisingly, incorporating a NaYF 4 inert shell as an isolating layer results in a remarkable 13.7-fold enhancement of upconversion luminescence in the NaYF 4 :Yb 3+ /Er 3+ @NaYF 4 /Au@Ag composite structure relative to isolated NaYF 4 : Yb 3+ /Er 3+ nanoparticles. The enhancement mechanism was carefully discussed based on spectral characteristics and luminous lifetime. It was found that the enhanced upconversion emission of nanoparticles on differently structured substrates is primarily driven by excitation enhancement. The composite rare-earth and metal nanoparticle structures fabricated in this investigation not only effectively enhance material spectral properties effectively but also streamline the process by employing an inert NaYF 4 shell as an isolation layer. This novel approach offers a promising avenue for developing efficient luminescent material systems.
研究领域:
材料科学
·
光学
·
纳米技术
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产品关联
DOI
高影响因子
TALANTA
·
2021
·
IF 6.057
Bo-Wen Liu,Pengcheng Huang,Fang-Ying Wu
Hypoxia refers to the lack of oxygen supply to cells or tissues. The overexpression of nitroreductase has been shown to be closely related to the degree of hypoxia, which leads to the level of nitroreductase (NTR) being used as an indicator of hypoxia. We reported a facile visual detection of NTR based on the aggregation of gold and silver alloy nanoparticles . Compared with gold nanoparticles (AuNPs), the aggregation behavior of Au 80 Ag 20 NPs caused a more prominent color change. Copper ions (Cu 2+ ) can be rapidly reduced by nicotinamide adenine dinucleotide (NADH) under the catalysis of Au 80 Ag 20 NPs. But NADH is consumed as an electron donor during the catalytic reduction reaction of p-nitrophenol (pNP) by NTR. A decrease of NADH amount results in the aggregation of Au 80 Ag 20 NPs by the excess Cu 2+ and different aggregation degrees of Au 80 Ag 20 NPs lead to observable color change. A linear correlation of A 600 /A 505 = 0.0285 [NTR]+0.361 (R 2 = 0.980) was obtained with a limit of detection (LOD) of 0.23 μg/mL for UV–vis spectrophotometer. For visual detection, the values of R/B against the concentration of NTR obtains a calibration curve of R/B = −0.031 [NTR]+ 1.54 (R 2 = 0.985) with a LOD of 0.76 μg/mL, which is of the same order of magnitude as the UV–vis spectrophotometer analysis. As a comparison, Au 80 Ag 20 NPs was replaced by several different composition nanoparticles (Au NPs, Au 70 Ag 30 NPs, Au 50 Ag 50 NPs) to be a chromogenic substrate, and the results suggest the Au 80 Ag 20 NPs is the most sensitive substrate in our assay. Selectivity tests showed that the detection system did not respond to other common substances, and the reaction mechanism was verified by inhibitor research. Finally, the assay was used on the human serum samples with spiking NTR, and the recovery rates of this assay with UV–vis spectrophotometer were basically consistent with RGB analysis.
研究领域:
生物化学
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医学检测
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纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2021
·
IF 4.098
Wenxian Wei,Dan Yu,Yu Du,Yicheng Ding,Qingli Huang
Au-Ag alloy nanoparticles (NPs) with controllable size and composition were synthesized by a facile, one-pot hydrothermal method. Various characterization techniques including TEM, UV–vis, EDX, HAADF-STEM and XPS were used to discuss the influencing factors for the size and composition of Au-Ag alloy NPs. It is obvious that the size and composition of Au-Ag alloy NPs could be adjusted by the experimental parameters. Catalytic and SERS performance of the Au-Ag alloy NPs were further investigated. Ideal catalytic and SERS performance could be also obtained via optimizing the size and composition of Au-Ag alloy. This work is of importance in theory research and practical application of the noble metal nanocomposites.
研究领域:
材料科学
·
化学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）