FOOD CHEMISTRY
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2026
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IF 10.4
Rui Zhang,Biying Guo,Xin Zhou,Fuyu Yang,Yong Xu
Simultaneous separation of structurally similar flavonoid glycosides remains challenging in functional food analysis. This study investigated the migration behavior of eight flavonoid glycosides by capillary zone electrophoresis with UV detection (CZE-UV). Phenolic deprotonation governed effective charge and electrophoretic mobility. However, strong pH dependence reduced robustness, prompting the exploration of less charge-dependent strategies. Deep eutectic solvents (DESs) provided limited improvement, whereas methyl-β-cyclodextrin (Me-β-CD) enabled stereoselective host-guest recognition and separated all eight analytes within 12 min under optimized conditions (5.0 mM Me-β-CD). The method showed good linearity and an LOD of approximately 0.3 μg/mL, supporting its use in quality control of purified moringa leaf extracts and related functional food materials. It quantified isoquercitrin and hyperoside as major flavonoid glycosides and detected two trace peaks tentatively assigned to spiraeoside and isovitexin with LC-MS/MS support. Download: Download high-res image (170KB) Download: Download full-size image
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PLANT PHYSIOLOGY AND BIOCHEMISTRY
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2025
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IF 5.7
Jiafei Qian,Wenwen You,Yicheng Wang,Yunlin Cao,Xiaoyong Zhao,Hongxia Xu,Hang Ge,Xiaoying Li,Di Wu,Junwei Chen,Xian Li
Loquat ( Eriobotrya japonica ) is rich in flavonols with health-promoting functions. However, regulatory mechanisms of flavonol biosynthesis in loquat are unclear. This study functionally characterized an R2R3-MYB, EjMYBF1 from loquat. Expression level of EjMYBF1 is positively correlated with flavonol content at different stages of fruit development and in ten accessions with differential flavonol accumulation. Dual-luciferase assays and EMSA indicated that EjMYBF1 transactivated the promoters of structural genes involved in flavonol biosynthesis by binding to promoter regions containing MYBCORE elements. Transient overexpression and virus-induced gene silencing assays of EjMYBF1 in loquat fruit peels and leaves, as well as heterologous constitutive expression of EjMYBF1 in Nicotiana tabacum confirmed that EjMYBF1 is a positive regulator of flavonol biosynthesis in loquat. These results demonstrate that EjMYBF1 is a functional flavonol-specific activator in loquat and explain one reason for its high flavonol content from the perspective of transcriptional regulation. This work provides a potential target for improving loquat nutrition and for applications in secondary metabolic engineering.
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JOURNAL OF FOOD COMPOSITION AND ANALYSIS
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2026
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IF 4.6
Zhihao Yang,Tianle Shen,Jinhu Tian,Yanbin Wang,Xingqian Ye,Jianchang Jin,Dan Wu
The determination of phenolic compounds serves as a critical approach for food quality control and screening of bioactive phytochemicals. This study developed a high-performance liquid chromatography-photodiode array detection (HPLC-PDA)-based analytical technique for plant phenolics, enabling simultaneous accurate quantification of 37 representative phenolic compounds in samples. Method validation demonstrated excellent linearity, precision, and accuracy, with intra-day and inter-day precision below 3 %, and spiked recoveries ranging from 96.88 % to 103.75 %. The methodology was successfully applied to analyze 19 distinct plant specimens, confirming its exceptional versatility. This method can be used as an efficient assay for plant polyphenols in multiple fields including food analysis, phytochemical research, and bioactive component development.
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Food Bioscience
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2023
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IF 5.2
Huiqun Fan,Mingshun Chen,Taotao Dai,Lizhen Deng,Chengmei Liu,Wei Zhou,Jun Chen
This study determined the phytochemical composition and bioactivity (antioxidant and hypoglycemic) in five fractions (petroleum ether, chloroform , ethyl acetate, n -butanol, and water fractions) obtained from ethanol extract of Amomum tsaoko Crevost et Lemaire ( A. tsaoko ). Qualitative and quantitative analyses of phenolic compounds were carried out with UHPLC-ESI-QTOF-MS/MS. 50 bioactive compounds were identified from five different fractions, including 11 phenolic acids and derivatives, 18 flavonoids and derivatives, 14 proanthocyanidins , 2 organic acids, and 5 other compounds. It was found that ethyl acetate fraction (EF) was enriched with the most types and contents of substances. Flavonoids and derivatives (including epicatechin and catechin) were the primary phenolic compounds in EF. In addition, EF demonstrated excellent antioxidant activity (DPPH and ABTS with IC 50 = 0.17 and 0.07 mg/mL, FRAP of 546.10 mg VCE/g DW) and anti-α-glucosidase activity (IC 50 = 20.14 μg/mL). EF could ameliorate fasting blood glucose and glucose tolerance of diabetic mice. In conclusion, EF could be developed as a potential candidate for preventing or treating oxidative stress diseases and diabetes.
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JOURNAL OF CHROMATOGRAPHY A
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2022
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IF 4.601
Zhixu Chen,Jincai Wang,Jiaming Yuan,Zhen Wang,Zhengchao Tu,Jacques Crommen,Wenhui Luo,Jialiang Guo,Tingting Zhang,Zhengjin Jiang
In this study, an advanced at-line nanofractionation based screening platform was developed to screen potential neuraminidase inhibitors (NAIs) from Lonicera japonica Thunb by involving two parallel bioassays, for determining both oseltamivir-sensitive neuraminidase (NAS) and oseltamivir-resistant neuraminidase (NAR) inhibitory activities. 20 potential NAIs with both NAS and NAR inhibitory effects were screened from Lonicera japonica Thunb and identified by mass spectrometer, including 11 phenolic acids, 8 flavonoids and one iridoid glycoside. The proposed at-line nanofractionation based screening platform for NAIs was also used to rapidly screen nine batches of water extracts of Lonicera japonica Thunb or its similar species. Clear differences in the number and content of active components were easily observed, demonstrating that the proposed method possesses great potential for the quality control of herb medicines.
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FOOD CHEMISTRY
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2023
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IF 8.8
Zhixin Xiao,Min Liu,Wentao Bi,David Da Yong Chen
In-depth studies of the extraction mechanism using deep eutectic solvents (DES), especially extraction through the formation of a deep eutectic system (DESys), revealed commonalities between the DES- and ionic liquids (IL)-based extraction systems. New applications of ILs and DES for extraction of nutritional natural products were presented. In this study, the extraction behavior of choline chloride (ChCl) and 1-(2-hydroxyethyl)-3-methylimidazolium chloride ([HMIm][Cl]) in DES and IL, respectively, in mechanochemical extraction of target compounds from Moringa oleifera leaves was systematically studied. The results suggested that both extraction methods were based on the formation of a DESys, either a normal DESys or an IL DESys. Considering the DESys-based one-step extraction improves the extraction efficiency and reduces the preparation time, the same idea can be used in IL for performance improvement. By formation of a new IL deep eutectic system based on hydrogen bond interaction in extraction, similar improvement was obtained.
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Food Science and Human Wellness
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2023
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IF 7
Linzi Li,Xueying Lei,Lin Chen,Ya Ma,Jun Luo,Xuebo Liu,Xinglian Xu,Guanghong Zhou,Xianchao Feng
Quercetin compounds have antioxidant, anti-inflammatory and anticancer pharmacological functions. Long-term exposure to acrylamide (AA) can cause liver injury and endanger human health. However, whether quercetin compounds can attenuate AA-induced liver injury and the specific mechanism are not clear. Here, we studied the mechanism and structure-activity relationship of quercetin compounds in reducing AA-induced hepatotoxicity in vivo and in vitro . In vivo studies found that quercetin-like compounds protect against AA-induced liver injury by reducing oxidative stress levels, activating the Akt/mTOR signaling pathway to attenuate autophagy, and improving mitochondrial apoptosis and endoplasmic reticulum stress-mediated apoptosis. In vitro studies found that quercetin compounds protected HepG2 cells from AA by attenuating the activation of AA-induced autophagy, lowering reactive oxygen species (ROS) levels by exerting antioxidant effects and thus attenuating oxidative stress, increasing mitochondrial membrane potential (MMP), and improving apoptosis-related proteins, thus attenuating AA-induced apoptosis. Furthermore, the conformational differences between quercetin compounds correlated with their protective capacity against AA-induced hepatotoxicity, with quercetin showing the best protective capacity due to its strongest antioxidant activity. In conclusion, quercetin compounds can protect against AA-induced liver injury through multiple pathways of oxidative stress, autophagy and apoptosis, and their protective capacity correlates with antioxidant activity.
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BIOMEDICAL CHROMATOGRAPHY
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2022
·Vol. 37
·期 2
·e5536
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IF 1.911
Qi Dong,Na Hu,Huilan Yue,Honglun Wang,Yue Wei
α -glucosidase inhibitors (AGIs) are widely used for the treatment of type 2 diabetes, but their side effects have made it to develop novel and alternative AGIs immediately. In this study, the extract of Hypericum perforatum L. (HPE) has been confirmed to have α -glucosidase inhibitory activity in vitro and in vivo. Seven active compounds, rutin, hyperoside, isoquercitrin, avicularin, quercitrin, quercetin, and biapigenin, were screened based on a bio-affinity chromatography column with α -glucosidase enzyme-conjugated solid phase and UPLC/MS, which exhibited excellent α -glycosidase inhibitory effects by the determined IC 50 values. The mechanism of α -glycosidase inhibitory activity of biapigenin was studied for the first time. The results showed that biapigenin was a high-potential, reversible, and mixed enzyme inhibitor. Analysis by molecular docking further revealed that hydrophobic interactions were generated by interactions between biapigenin and amino acid residues LYS156, PHE303, PHE314, and LEU313. In addition, hydrogen bonding occurred between biapigenin and α -glucosidase amino acid residues ASP307, SER241, and LYS156. This research identified that biapigenin could be a novel AGI and further applied to the development of potential anti-diabetic drugs. Furthermore, our studies established a rapid in vitro screening method for AGIs from plants.
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FOOD CHEMISTRY
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2022
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IF 9.231
Yuan Pan,Hui-Wen Gu,Yi Lv,Xiao-Li Yin,Ying Chen,Wanjun Long,Haiyan Fu,Yuanbin She
Identifying geographical origins of red wines made in specific regions is of significance since the false claim of geographical origins has been frequently exposed in China's wine industry. In this work, an untargeted metabolomic approach based on UPLC-QTOF-MS was established to discriminate geographical origins of Chinese red wines. The principal component analysis (PCA) showed significant differences between wine samples from three famous geographical origins in China. The metabolites contributing to the differentiation were screened by orthogonal partial least squares-discriminant analysis (OPLS-DA) with pairwise modeling. 40 and 46 differential metabolites in positive and negative ionization modes were putatively identified as chemical markers. Furthermore, heatmap visualization and OPLS-DA models were constructed based on these identified markers and external verification wine samples from different regions were successfully discriminated, with recognition rate up to 96.7%. This study indicated that UPLC-QTOF-MS-based untargeted metabolomics has great potential for the geographical origin traceability of Chinese red wines.
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Food & Function
·
2022
·Vol. 13
·期 9
·5061-5074
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IF 6.317
Jie Zhao,Lin Huang,Renjie Li,Zhuangwei Zhang,Jin Chen,Hongjin Tang
Gliadin, as a main component of wheat storage protein, is used as a drug encapsulation and delivery system owing to its specific characteristics. Flavonoids are regarded as active natural products with a variety of pharmacological effects. In this study, an integrated method including UV-vis, fluorescence, and FT-IR spectroscopy and molecular modelling was applied to explore the structure–affinity relationship and the interaction nature between a library of flavonoids and gliadin. The characteristic UV-vis spectral changes of gliadin mediated by flavonoids with absorption bands at 218 and 278 nm demonstrated the existence of an interaction depending on generating the ground-state complexes. Fluorescence quenching results showed that the intrinsic fluorescence of gliadin could be effectively quenched by flavonoids coupled with the formation of flavonoid–gliadin complexes through the static quenching mechanism. The structure–affinity relationship revealed the critical structural elements associated with the binding affinity on gliadin and underlined the favorable substituents at the specific positions of flavonoid skeletons leading to a stronger binding potency. From the analysis of synchronous fluorescence spectra, flavonoids could cause the conformation change of gliadin and impact the microenvironment around TYR and TRP residues. Moreover, the ANS fluorescent probe assay suggested that these flavonoids also influenced the surface hydrophobicity of glaidin based on the further exposure or blocking of hydrophobic domains. Molecular modelling was subsequently performed and illustrated the proposed binding conformation of flavonoids on gliadin. Combined with the FT-IR spectra, these results further confirmed the important role of hydrophobic interactions and hydrogen bonds in their binding process.
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CHEMOSPHERE
·
2022
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IF 8.943
Liangmei Rao,Yifu Zhu,Zhongshu Duan,Ting Xue,Xuemin Duan,Yangping Wen,Annamalai Senthil Kumar,Weiming Zhang,Jingkun Xu,Akbar Hojjati-Najafabadi
Biomass waste, a good candidate for advanced carbon materials for sustainable electrodes, is receiving more and more attention for high value-added materials because of its promising contribution to economic growth and sustainable development. We proposed a green co-hydrothermal approach to prepare lotus seedpods biochar (BC) decorated molybdenum disulfide (MoS 2 ) from waste lotus seedpods and precursors of MoS 2, and a portable, flexible, outdoor and inexpensive sensing platform for hyperin on the integrated flexible three-electrode using U-disk potentiostat with smartphone was successfully developed. Structure and properties of MoS 2 -BC were characterized, it was proved that BC improves microstructure and morphology, electronic conductivity, electrode stability and electrocatalytic properties of MoS 2 . Attributing to these impressive features, the detection signal of hyperin was significantly amplified by the MoS 2 -BC modified glass carbon electrode (GCE) in detection range of 0.01–21 μΜ with detection limit (LOD) of 5 nM. It was worth mentioning that the MoS 2 -BC modified screen-printed electrode (SPE) performs hyperin detection in range of 100 nM - 3 μM with LOD 50 nM (S/N = 3). The practicability of the proposed method confirmed that the portable, on-site, low-cost, and outdoor detection of hyperin was feasible and practical in comparison with traditional both electrochemical sensing and HPLC methods.
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POSTHARVEST BIOLOGY AND TECHNOLOGY
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2018
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IF 3.112
Yurong Ma,Qingfeng Ban,Jingying Shi,Tiantian Dong,Cai-Zhong Jiang,Qingguo Wang
The effects of 1-methylcyclopropene (1-MCP) treatment, storage time, and shelf life and temperature on phenolic concentrations and antioxidant activity in apple peel and flesh of ‘Jonagold’ apple ( Malus domestica Borkh) have been investigated. Hyperoside and chlorogenic acid were the most abundant phenolic compounds in the peel and flesh, respectively. The flesh had lower concentrations of individual phenolics, total phenolics, flavonoids and lower antioxidant activity (determined by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay) than the peel. In addition, the profiles of phenolics as well as the changing patterns of individual phenolics also differed between the peel and the flesh. After long-time storage (180 d), the greatest decrease of concentrations was the concentrations of hyperoside (27 %) in the peel and total phenolics (35 %) in the flesh. Also, during the 90–180 d storage period, hyperoside and rutin in the peel, chlorogenic acid in the flesh, and flavonoids and antioxidant activity decreased in both peel and flesh tissues. Individual phenolics exhibited different stabilities during the early stages after harvest. 1-MCP reduced the reduction in the concentrations of hyperoside in the peel, chlorogenic acid in the flesh, and total phenolics, flavonoids and antioxidant activity in both the peel and flesh tissues during storage and shelf life. However, for quercetin and rutin, the protective effect of 1-MCP was only observed during the shelf life period. Overall, phenolic compounds responded variously to storage, shelf life, shelf temperature and 1-MCP treatment.
研究领域:
食品科学
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植物生理学
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危险品化学品经营许可证（带存储）