MICROCHEMICAL JOURNAL
·
2023
·
IF 4.8
Yang Mei,Si Yang,Chenxi Li,Xinyu Li,Yuewen Yu,Rong Liu,Wenliang Chen,Xiaoli Wang,Kexin Xu
Mercury ion (Hg 2+ ) detection plays a crucial role in safeguarding the environment and protecting human health. In this study, we developed a microfluidic biosensing platform that utilizes a phosphorothioate-RNA (PS-RNA) probe and gold-coated magnetic nanoparticles to quantify Hg 2+ visually. The PS-RNA probe was linked to gold-coated magnetic nanoparticles via Au–S interaction and biotin–avidin interaction and then combined with polystyrene microspheres to form a self-assembled structure called a magnetic bead-AuNP-probe-polystyrene (MAPP) bead. We designed and fabricated a microfluidic chip that contained only a magnetic separator and a particle trap. The presence of Hg 2+ cut the PS-RNA probe, leading to the separation of magnetic beads and polystyrene microspheres. The MAPP was removed by the magnetic separator, while the cut polystyrene microspheres flowed along the channel and accumulated in the particle trap, forming a long visible strip. The length of the polystyrene microsphere strip was proportional to the Hg 2+ concentration. The microfluidic biosensing platform exhibited high sensitivity and specificity for Hg 2+ detection, with a detection limit of 21.9 nM. By integrating the microfluidic magnetic manipulation system with a sensitive nucleic acid probe, we developed an automated and visual biosensor that is a valuable reference for the on-site visual detection of heavy metal ions.
研究领域:
环境科学
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分析化学
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生物技术
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纳米技术
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Nano Materials
·
2022
·
IF 6.14
Baichuan Zhao,Huihui Liu,Lingzhi Xia,Zhi Wang,Congyun Zhang
The development of powerful technologies to simultaneously monitor and eliminate toxic organic pollutants is a vital focus of environmental research. For this purpose, a recyclable surface-enhanced Raman scattering (SERS) substrate was successfully prepared by successively depositing gold nanoparticles (AuNPs) and a reduced graphene oxide (rGO) film on TiO2 microflower particles by two-step in situ reduction processes. The prepared TiO2@Au-rGO ternary composites exhibit not only good SERS performance via molecule enrichment of rGO and surface plasmon resonance (SPR) capability, but also enhanced photocatalytic degradation activity because of the increased broadband UV–visible-light-harvesting efficiency and SPR-enhanced charge transfer. The detection limit of rhodamine 6G (R6G) as low as 10–11 M and excellent reproducibility were achieved. Additionally, the TiO2@Au-rGO ternary composites yield complete removal of R6G within 15 min under broadband UV–visible light illumination, wherein their apparent reaction-rate constant is 2.8 and 5.5 times larger than those of binary TiO2@Au and pure TiO2, respectively. Meanwhile, no distinct SERS and photocatalytic activity loss were observed even after five reusability tests, achieving the recycling SERS application by photocatalytic degradation. Most importantly, the as-fabricated ternary samples can be explored for the efficient capture, sensitive detection, and self-cleaning degradation of 2,2′,4,4′-tetrabromodiphenyl ether (BDE-47), revealing great potential applications in the SERS-based simultaneous monitoring and removal of trace aromatic persistent organic pollutants.
研究领域:
环境科学
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材料科学
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分析化学
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纳米技术
✓ 已验证
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2023
·
IF 9.5
Xiaowei Cao,Shengjie Ge,Miao Chen,Haiyan Mao,Ying Wang
Multiplexed detection of Parkinson’s disease (PD) biomarkers is of great significance for early diagnosis and personalized treatment. In this study, we fabricated a robust surface-enhanced Raman scattering-enabled lab-on-a-chip (LoC-SERS) platform for simultaneous quantification of α-synuclein, phosphorylated tau protein 181, osteopontin, and osteocalcin. Herein, the antibody–DNA conjugate was designed to introduce the catalytic hairpin self-assembly (CHA) amplification into the protein detection. Au nano-stars (AuNSs) modified with Raman reporter molecules and hairpin-structure DNA 1 were applied as the SERS nanotags. Au-coated silicon nanocone array (Au/SiNCA) fabricated based on the maskless plasma etching-prepared high-density Si nanocone array (SiNCA) and surface ion sputtering was used as the capture substrate after the modification of hairpin-structure DNA 2. Benefitting from the antibody–DNA conjugate-induced CHA amplification, numerous AuNSs can be connected to the Au/SiNCA surface, which significantly amplify the plasmonic coupling effect for ultrasensitive SERS detection, and the limit of detection was less than the pg/mL level. The application of highly uniform Au/SiNCA and antibody–DNA conjugate endows the LoC-SERS platform excellent analytical performance, including superior reproducibility, satisfactory universality, and high sensitivity. In addition, a 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced PD mice model was established, and satisfactory results were obtained in real sample analysis with the LoC-SERS platform, which may be enlightening for exploiting protein biomarkers in PD monitoring.
研究领域:
生物医学工程
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分析化学
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神经科学
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疾病诊断
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2020
·Vol. 413
·期 1
·235-244
·
IF 3.637
Guo Haixin,Su Xiaoping,Su Qingfu,Zhuang Wei,You Zhijiao
A portable photothermal immunoassay based on Au-coated magnetic Fe 3 O 4 core-shell nanohybrids (Au-Fe 3 O 4 ) was developed for point-of-care (POC) testing of lipoprotein-associated phospholipase A 2 (Lp-PLA 2 ) on a digital near-infrared (NIR) thermometer. Au-Fe 3 O 4 photothermal materials were first synthesized through reverse micelle method, and then functionalized with polyclonal rabbit anti-human Lp-PLA 2 antibody. A sandwiched immunoreaction was carried out in polyclonal mouse anti-human Lp-PLA 2 antibody-coated microplate using Au-Fe 3 O 4 -labeled antibody as the detection antibody. With formation of sandwich-type immunocomplex, the captured Au-Fe 3 O 4 on the plate converted the light into heat under an 808-nm laser irradiation (1.5 W cm −2 ), thereby resulting in the increasing temperature of the detection solution. The temperature variations relative to surrounding temperature was determined on a portable NIR thermometer. Several labeling protocols with gold nanoparticle, Fe 3 O 4 nanoparticle, or Au-Fe 3 O 4 nanohybrids were investigated for determination of Lp-PLA 2 and improved analytical features were achieved with the core-shell Au-Fe 3 O 4 nanohybrids. Under optimum conditions, Au-Fe 3 O 4 -based immunoassay exhibited good photothermal responses for the detection of Lp-PLA 2 with a dynamic linear range of 0.01–100 ng mL −1 at a low detection limit of 8.6 pg mL −1 . Good reproducibility and intermediate precision were less than 9.7%. Other biomarkers or proteins did not interfere with responses of this system. An acceptable accuracy was acquired for analysis of human serum sample between Au-Fe 3 O 4 -based photothermal immunoassay and commercialized human Lp-PLA 2 ELISA kit.
研究领域:
纳米技术
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生物医学工程
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分析化学
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即时检测
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免疫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）