CHINESE JOURNAL OF CHEMICAL ENGINEERING
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2021
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IF 3.171
Lutai Song,Li Liu,Mingyuan Zhu,Bin Dai
The continuous expansion of vinyl chloride production increases environmental pollution caused by mercury catalysts, which is an issue that urgently needs to be solved. Green and stable catalysts should be researched to alleviate this issue. In this research, Thiolactic acid acts as a ligand where sulfhydryl groups form a stable complex with Au on the surface of a spherical activated carbon (SAC). An Au-thiolactic acid/SAC catalyst was designed with a Au theoretical loading of 0.5% (mass) to overcome the disadvantages of traditional Au-based catalysts, such as a low conversion rate and poor life cycle. The ratio of Au to ligand was screened, and the activity was best when Au/S = 1:8. The formation of the Au-S bond was proven by FT-IR and UV–vis. The longevity test of the Au1S8 /SAC catalyst was carried out at 1200 h −1 for 50 h. Samples with reaction times of 0 h, 5 h, 10 h, 20 h, and 50 h were taken to monitor the catalyst status. The XPS and TPR tests proved that the Au-S bond broke as the acetylene hydrochlorination reaction proceeded. The DFT calculation proved that the Au-S bond is the active site, and the sulfur atom promotes the adsorption of C 2 H 2 by the catalyst.
研究领域:
化学工程
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环境科学
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产品关联
DOI
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
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2025
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IF 4.1
Xuanxuan Liu,Qingliu Wu,Yong Cao,Lu Wang,Hao Qu,Lei Zheng
l -cysteine plays a vital role in both the nutritional value and taste profile of chicken broth, underscoring the necessity for an accurate and sensitive approach to detect and measure l -cysteine at minute concentrations. A reliable method for identifying trace amounts of l -cysteine is imperative for ensuring the quality and authenticity of chicken broth products. Such a technique would enable precise control over the levels of this crucial amino acid, ultimately contributing to the overall consumer satisfaction and product consistency. This research focused on developing an SGGT-based sensor for the rapid detection of l -cysteine by leveraging the self-assembly of l -cysteine molecules on the surface of the Au gate electrode to form covalent Au-S bonds. This process triggers a rearrangement of charge carriers on the surface, leading to a shift in the electric double layer potential. Through field effect amplification, a substantial channel current response is generated. This innovative sensing mechanism effectively addresses the selectivity challenges of SGGT in complex liquid environments, significantly enhancing its ability to detect l -cysteine. Subsequently, the study examined three different gate voltages, with the optimal voltage achieving a detection limit of 10 nM (1.21 ng/mL) and a linear detection range of 30 nM – 3 μM. Moreover, the sensor exhibits highly selective detection of l -cysteine in chicken broth, demonstrating an 85.87 % recovery rate. The innovative technology demonstrates encouraging prospects for real-world usage in the identification of l -cysteine in food items or as a biomarker. Its capabilities offer a reliable method for detecting this amino acid, showcasing its versatility and potential impact on food safety and quality assessment.
研究领域:
食品科学
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生物传感
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材料科学
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DOI
高影响因子
MICROCHEMICAL JOURNAL
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2022
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IF 5.304
Lizhu Fu,Yujiao Hou,Haipeng Guo,Chang Liu,Ying Li,Boyu Jia,Lidong Zhou,Weijun Kong
Development of highly efficient and sensitive methods for trace detection of pesticides in foods is in great need. In this study, a signal “on–off-on” fluorescent nanosensor was proposed based on gold nanoparticles (AuNPs) and biomass derived nitrogen-doped carbon dots (N-CDs) for accurate qualitation and quantitation of thiram in hawthorn. The water-soluble orange peel-derived N-CDs were obtained through a facile one-step microwave-assisted synthesis strategy. The fluorescence of biomass N-CDs could be effectively quenched by AuNPs through inner filter effect (IFE), and recovered when AuNPs were aggregated. In the presence of thiram, N-CDs specially bound with AuNPs through Au-S bonds, causing the aggregation of AuNPs with a visible color change from red to blue, accompanying with the release of N-CDs and a recovery of fluorescence. The restored fluorescence intensity was relevant with the added amount of thiram in a concentration-dependent manner for reliable quantitation. Under the optimized conditions, the newly-developed fluorescent nanosensor exhibited high selectivity for thiram out of other six interfering pesticides, as well as excellent sensitivity with an ultralow detection limit of 4.7 ng/mL and a wide detection range of 10–200 ng/mL. The practical application of the established nanosensor in the spiked hawthorn samples confirmed satisfactory recoveries of 102.22–107.57% with relative standard deviations (RSDs) lower than 5%. The results indicated the feasibility, practicality and wide application prospect of the proposed fluorescent nanosensor for thiram in foods and other commodities.
研究领域:
分析化学
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纳米技术
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食品科学
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环境科学
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产品关联
DOI
高影响因子
TALANTA
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2020
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IF 5.339
Xiaofei Li,Yawen Zhang,Lulu Hao,Yanju Liu,Xia Wang,Huaixia Yang,Jinming Kong
Cytokeratin fragment antigen 21-1 (CYFRA 21-1) DNA is perceived as sensitive tumor marker for the diagnosis of non-small cell lung cancer and other tumor. Herein, linear chain poly(ε-caprolactone) (PCL) synthesized by ring-opening polymerization is applied to ultrasensitive label-free electrochemical impedance detection system for CYFRA 21-1 DNA. First, thiolated peptide nucleic acid (PNA) is self-assembled into the Au electrode surface through the formation of Au–S bonds, allowing the PNA to act as biomolecular probe and form PNA/DNA heteroduplex with the target DNA via specific hybridization. Then, PCL is conjugated to the immobilized DNA on the electrode via “carboxylate–Zr 4+ –phosphate” bridges. Finally, the electrochemical response of modified PNA/DNA/Zr 4+ /PCL electrode is determined by electrochemical impedance method to quantify of CYFRA 21-1 DNA. Under optimal conditions, this method exhibits highly sensitivity with a broad linear range (0.1 fM – 1 nM) ( R 2 = 0.995) and the limit of detection (LOD) is as low as 10.73 aM, which is equivalent to just 64 molecules in a 10 μL sample. What's more, the high selectivity , good anti-interference, label-free operation, and real-time monitoring in complex samples of the proposed strategy demonstrate its broad application for the early diagnosis and clinical monitoring.
研究领域:
生物医学工程
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分析化学
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分子生物学
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癌症诊断
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产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
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2018
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IF 5.123
Kunhao Yang,Zhongqiu Li,Yueguang Lv,Chuyi Yu,Peilong Wang,Xiaoou Su,Li Wu,Yujian He
An electrochemical aptasensor for ultrasensitive detection of hydroxylated polychlorinated biphenyl (OH-PCB) was developed. In this work, the sulfydryl aptamer selected for OH-PCB was easily immobilized on the surface of the modified glassy carbon electrode via Au-S bond due to incorporation of gold nanoparticles/poly dimethyl diallyl ammonium chloride-graphene composite. The fabrication process of the electrochemical aptasensor was characterized by electrochemical impedance spectroscopy and cyclic voltammetry . The peak currents obtained by differential pulse voltammetry decreased with the increasing of OH-PCB concentrations. The current responded approximately logarithmically to the OH-PCB concentration ranging from 2.9 × 10 −11 M to 2.9 × 10 −7 M with excellent linear correlation performance. The detection limit of OH-PCB was estimated as low as 5.3 × 10 −12 M. The developed method had been successfully applied to analyzing lake water. Particularly, the method of the electrochemical aptasensor opens up a new perspective in environmental monitoring by the aptamer identification.
研究领域:
环境科学
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分析化学
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纳米技术
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电化学
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产品关联
DOI
PubMed
高影响因子
TALANTA
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2020
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IF 5.339
Libo Li,Bainian Chen,Lijun Luo,Xiaohong Liu,Xiaoya Bi,Tianyan You
In the present study, a novel self-enhanced electrochemiluminescence (ECL) aptasensor which combined self-enhanced ECL composite as signal response element and aptamer as the specific recognition element was firstly proposed for the sensitive and selective detection of Hg 2+ . Innovatively, the luminophore Ru(bpy) 3 2+ -doped silica nanoparticles functionalized with 3-aminopropyltriethoxysilane (NH 2 – [email protected] 2 ) and the co-reactant nitrogen doped graphene quantum dots (NGQDs) were bound together to form the self-enhanced ECL composite, NH 2 – [email protected] 2 -NGQDs, by electrostatic adsorption. Satisfactorily, high and stable luminous efficiency of NH 2 – [email protected] 2 -NGQDs was obtained benefited from the short electron transfer distance and low energy loss of self-enhanced-typal ECL composite. For the fabrication of the self-enhanced ECL aptasensor for Hg 2+ detection, the Hg 2+ aptamer, of which one end was connected to NH 2 – [email protected] 2 -NGQDs, and the other end was fixed on glass carbon electrode (GCE) surface through Au–S bond, was served as a bridge. Upon the addition of Hg 2+ , the aptamer was bent due to the formation of thymine-Hg 2+ -thymine (T-Hg 2+ -T) specific structure, which caused the self-enhanced ECL composite was close to the GCE surface. On this basis, a linearly enhanced ECL signal was acquired with the concentration of Hg 2+ in the range of 5.0 × 10 −11 M − 1.0 × 10 −6 M with excellent selectivity , repeatability and stability in 3 min for each assay. In addition, the proposed aptasensor showed satisfying accuracy and practicability for Hg 2+ analysis in tap and canal water verified by the inductively coupled plasma-mass spectrometry (ICP-MS) method.
研究领域:
分析化学
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环境科学
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纳米技术
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电化学
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产品关联
DOI
PubMed
高影响因子
APPLIED SURFACE SCIENCE
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2020
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IF 6.182
Ruijiang Liu,Shuai Pan,Min Liu,Wei Huang,Zhixiang Lv,Aolin He
A label-free electrochemical biosensor with magnetically induced self-assembly based on peptide nucleic acids (PNA) and Fe 3 O 4 /Fe 2 O 3 heteroplasmon nanorods was successfully fabricated to detect the single nucleotide polymorphism (SNP) of CYP2C9*3. The sensitivity of the electrochemical biosensor was improved by introducing PNA and Fe 3 O 4 /Fe 2 O 3 @Au nanocomposites. The Fe 3 O 4 /Fe 2 O 3 @Au nanocomposites were assembled on the surface of the magnetic glassy carbon electrode (MGCE) by magnetic field, and the thiolated PNA were connected to the nanocomposites through Au-S bonds. The biosensor exhibited a good linear correlation between 1 pM and 1 µM (R 2 = 0.9941), and the limit of detection (LOD) and the limit of quantitation (LOQ) were 0.95 pM and 3.18 pM, respectively. Besides, the electrochemical biosensor could recognize single-base mismatch DNA, and it could be stored stably for 15 days at 4 °C.
研究领域:
生物传感器
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电化学
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分子诊断
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纳米技术
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遗传学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）