Biosensors-Basel
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2023
·Vol. 13
·期 6
·585
·
IF 5.4
Yunping Lai,Beirong Yu,Tianran Lin,Li Hou
A multicolor visual method for the detection of hydrogen peroxide (H2O2) was reported based on the iodide-mediated surface etching of gold nanostar (AuNS). First, AuNS was prepared by a seed-mediated method in a HEPES buffer. AuNS shows two different LSPR absorbance bands at 736 nm and 550 nm, respectively. Multicolor was generated by iodide-mediated surface etching of AuNS in the presence of H2O2. Under the optimized conditions, the absorption peak Δλ had a good linear relationship with the concentration of H2O2with a linear range from 0.67~66.67 μmol L−1, and the detection limit is 0.44 μmol L−1. It can be used to detect residual H2O2in tap water samples. This method offered a promising visual method for point-of-care testing of H2O2-related biomarkers.Keywords:iodide;gold nanostar;H2O2
研究领域:
分析化学
·
生物医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2015
·
IF 6.409
Sai Bi,Xiaoqiang Jia,Jiayan Ye,Ying Dong
Gold nanomaterials promise a wide range of potential applications in chemical and biological sensing, imaging, and catalysis. In this paper, we demonstrate a facile method for room-temperature synthesis of gold nanostars (AuNSs) with a size of ~50 nm via seeded growth. Significantly, the AuNSs are found to have high light-scattering properties, which are successfully used as labels for sensitive and selective detection of nucleic acids and proteins by using exonuclease III (Exo III) as a biocatalyst . For DNA detection, the binding of targets to the functionalized AuNS probes leads to the Exo III-stimulated cascade recycling amplification. As a result, a large amount of AuNSs are released from magnetic nanoparticles (MNPs) into solution, providing a greatly enhanced light-scattering signal for amplified sensing process. Moreover, a binding-induced DNA three-way junction (DNA TWJ) is introduced to thrombin detection, in which the binding of two aptamers to thrombin triggers assembly of the DNA motifs and initiates the subsequent DNA strand displacement reaction (SDR) and Exo III-assisted cascade recycling amplification. The detection limits of 89 fM and 5.6 pM are achieved for DNA and thrombin, respectively, which are comparable to or even exceed that of the reported isothermal amplification methods. It is noteworthy that based on the DNA TWJ strategy the sequences are independent on target proteins. Additionally, the employment of MNPs in the assays can not only simplify the operations but also improve the detection sensitivity. Therefore, the proposed amplified light-scattering assay with high sensitivity and selectivity , acceptable accuracy, and satisfactory versatility of analytes provides various applications in bioanalysis .
研究领域:
生物传感
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分析化学
·
纳米生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MATERIALS CHEMISTRY AND PHYSICS
·
2025
·
IF 4.7
Xue-Jie Zhao,Lin-Song Li,Peng-Wei Chen,Dong Cheng,Bang-Bang Liu,Mei-Xia Zhao
So far, the single treatment has failed to achieve the desired outcomes in cancer treatment, significantly contributing to the surge in interest in multimodal synergistic therapies for cancer. In this study, we developed a multifunctional biomimetic nanoplatform (GNS@PDA-Pt-DOX-9R-P201, GPPtD9R NPs), which consists of photothermal reagents gold nanostar (GNS), biomimetic material polydopamine coating (PDA), targeting peptide (9R-P201) and two kinds of chemotherapeutic agent doxorubicin (DOX) and cis-platinum (CDDP). The particle size of the GPPtD9R NPs was 255 ± 10.56 nm, the zeta potential was 3.75 ± 0.13 mV, the photothermal conversion efficiency was 21.33%, and the loading rates of DOX and CDDP were 5.98%% and 7.67%, respectively. Under 808 nm light irradiation, the GPPtD9R NPs elevated the temperature beyond the safe threshold for cells. The modification with 9R-P201 enabled targeted delivery, and upon laser irradiation, the GPPtD9R NPs released DOX and CDDP in the tumor, achieving the synergistic treatment of hepatocellular carcinoma (HCC) through photothermal therapy (PTT) and dual chemotherapy, which not only enhances the anti-tumor effect but also minimizes systemic toxicity. The platform achieved a remarkable 90.3% tumor suppression rate for HCC, and no abnormalities in biochemical blood indicators or damage to normal tissues were found at the end of the treatment. Overall, GPPtD9R NPs exhibit potent anti-HCC efficacy and exceptional biocompatibility, presenting a novel strategy for tumor therapy.
研究领域:
肿瘤学
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纳米医学
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生物材料科学
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Bio Materials
·
2020
·
IF 0
Dalin Wu,Pei Zhao,Lei Wu,Lihua Lin,Guoyi Yu,Linlin Xu,Jun Yue
Gold nanostars (AuNS) are promising carriers for targeted delivery of therapeutic oligonucleotides, but their potential in fabricating an on-demand drug release system in a facile and robust way remains to be explored. In this paper, we used a model aptamer (HApt), acting not only as a target ligand but also as a natural thermal-responsive material, to decorate AuNS. The prepared gold nanoconstruct, HApt@AuNS, displayed stoichiometric loading capacity of the anthracycline drug doxorubicin (Dox). The on-demand drug release was realized by illuminating nanoconstructs with near-infrared (NIR) light. Furthermore, a higher degree of Dox release from the nanoconstructs was achieved in an acidic environment, compared to neutral conditions. The in vitro experiments showed that Dox-intercalation did not affect the cell uptake efficiency of HApt@AuNS, which could enter cells through clathrin-mediated endocytosis and microtubule-dependent active transport to lysosomes. Dox-loaded HApt@AuNS exhibited intracellular on-demand drug release and enhanced toxicity against cancer cells by NIR-irradiation.
研究领域:
纳米医学
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癌症治疗
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生物材料
✓ 已验证
DOI
PubMed
FOOD CONTROL
·
2024
·
IF 5.6
Xinchen Wu,Limei Yin,Shipeng Gao,Ruiyun Zhou,Yang Zhang,Shanshan Xue,Heera Jayan,Hesham R. El-Seedi,Xiaobo Zou,Zhiming Guo
Patulin (PAT), a major contaminant in apples, poses a huge threat to human health as well as the economic sector. There is an urgent need to develop a sensitive, selective, and fast-responsive method to detect PAT in apples. However, one of the main challenges is overcoming the interferences of complex food matrices. In this study, we developed a highly sensitive competitive SERS sensor based on plasmonic nanoparticles modified by aptamers. The study utilized the formation of nanocomposites through aptamer-modified Au@Ag nanoparticles (NPs) and gold nano-stars (AuNSs) to induce high-intensity Raman signals from the SERS tag. Subsequently, in the presence of PAT, the nanocomposites underwent decomposition, evident through the significant decrease in SERS intensity. According to the standard curve established in this study, the detection limit was 0.0281 ng/mL. The competitive sensor was applied to spiked apple fruit and juice samples, indicating a recovery rate ranging from 91.98% to 102.94%. The excellent analytical performances and high sensitivity observed suggest the potential of the plasmonic nanocomposite sensing strategy in detecting PAT in real matrices.
研究领域:
食品科学
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纳米技术
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分析化学
✓ 已验证
产品关联
DOI
高影响因子
NANOTECHNOLOGY
·
2020
·Vol. 32
·期 8
·085102
·
IF 3.551
Yujie Cai,Luyao Wang,Haolu Hu,Wei Bing,Limei Tian,Jie Zhao
Herein, we successfully developed a new multifunctional antibacterial system, which combined mechano-bactericidal (Au-nanostars) and photothermal (MoS2) mechanism. Meanwhile, the targeting molecule of vancomycin was modified on the surface of MoS2–Au nanocomposites (Van-MoS2–Au), that generally yield high efficiency in antibacterial performance due to their effective working radii. Van-MoS2–Au nanocomposites were capable of completely destroying both gram-negative (E. coli) and gram-positive (B. subtilis) bacteria under 808 NIR laser irradiation for 20 min, and nearly no bacterial growth was detected after 12 h incubation. Moreover, these nanocomposites could destruct the refractory biofilm as well, which was a much more difficult medical challenge. The new antibacterial nanomaterials might offer many biomedical applications because of the biocompatibility and strong antibacterial ability.
研究领域:
生物医学工程
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材料科学
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抗菌研究
✓ 已验证
DOI
高影响因子
JOURNAL OF DRUG TARGETING
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2019
·
IF 3.277
Gaoxing Su,Dandan Miao,Yanyan Yu,Min Zhou,Peifu Jiao,Xiaolong Cao,Bing Yan,Hongyan Zhu
Combined chemo-photothermal therapy is attracting increasing attention in the treatment of cancers. In this work, PEGylated mesoporous SiO2-coated gold nanostars (GNS@mSiO2-PEG) were synthesised without using the cytotoxic surfactant cetyltrimethylammonium bromide as the template. Mesoporous nanostructures were obtained by poly(vinylpyrrolidone) protection of the outer silica shell and NaOH etching of the inner shell. GNS@mSiO2-PEG exhibited good dispersity in medium and excellent photothermal effects. Loading capacity for the anticancer drug doxorubicin (DOX) was ∼17.9%, and the drug release profile was pH- and light-responsive. In vitro studies revealed that the as-prepared nanocomposites featured good biocompatibility. Furthermore, the nanocomposites were readily internalised by cancer cells, and a combined chemo-photothermal therapy assay revealed that DOX-loaded GNS@mSiO2-PEG have a higher therapeutic efficiency than individual therapies, demonstrating suitable synergistic effects.
研究领域:
纳米医学
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肿瘤学
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生物材料学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Materials Today Communications
·
2018
·
IF 0
Jing Liu,Hongbo Cui,Siyu Yan,Xunan Jing,Daquan Wang,Lingjie Meng
We report a facile fabrication approach for sensitive theranostic nanoprobe - gold [email protected] 2 nanosheets, by which gold nanostars were grown on the surface of MnO 2 nanosheets. Owing to the ability of gold nanostars to absorb light at near infrared (NIR) wavelength, the nanoprobe showed excellent photothermal conversion effect, while exhibited tumor microenvironment-responsive magnetic resonance imaging property due to the redox property of MnO 2 nanosheets. Amazingly, photothermal irradiation experiment showed that the nanoprobe sensitively triggered lung cancer cell death, while produced negligible effect on normal lung epithelial cells. Therefore, these demonstrate that the nanoprobe may have potential application in magnetic resonance imaging-guided photothermal therapy of lung cancer.
研究领域:
纳米技术
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生物医学工程
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癌症研究
✓ 已验证
DOI
JOURNAL OF POWER SOURCES
·
2019
·
IF 7.467
Yan Wei,Xiao Zhang,Zhiguo Liu,Hsueh-Shih Chen,Ping Yang
Plasmonic-based catalysts are capturing widespread attention in the field of electrocatalysis , however, are still facing huge challenges in activity, stability, and mechanism interpretation. Herein, the unique AgPt alloy-modified multibranched Au nanostar heterostructures with wider tunable optical properties and desirable activities are precisely fabricated via selective deposition of Ag and Pt. AgPt-tipped Au nanostars have the most remarkable performance in electrocatalytic hydrogen evolution (overpotential of 58 mV at a current density of 10 mA cm −2 ) and methanol oxidation reaction (4.01 A mg N −1 ) driven by visible and near-infrared light irradiation , which are mainly ascribed to the effective transfer of plasmon-excited electron-hole pairs. Additionally, the increased system temperature generated by the photothermal effect of Au, larger active area, and modified electronic state also contribute to the enhancement of activity, stability, and tolerance of CO poisoning. As the adverse charge transport pathway, the electrocatalytic performances of AgPt-edged and -covered Au nanostars are inhibited. The experimental results reveal that rationally designed bifunctional plasmonic electrocatalysts which make full use of the synergy of photoelectric , photothermal, and structural effect have promising potential in photoelectric catalytic conversion fields.
研究领域:
材料科学
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电化学
·
光催化
✓ 已验证
DOI
高影响因子
Nanomaterials
·
2025
·Vol. 15
·期 8
·612
·
IF 4.3
Kang Yang,Fang Yang,Xiaoling Lu,Hao Li,Zeng Yang,Qi Yin,Lin Zhang,You Long,Chao Shen,Liya Chen,Bo Yao,Chenghong Huang
Simple and accurate analysis of cancer-related biomarkers is very important for disease screening and auxiliary diagnosis. This study proposed a facile immunoassay that used gold nanostar-labeled rabbit anti-AFP as a capture antibody and gold nanoparticle-conjugated goat anti-rabbit IgG as an enhance antibody for the construction of a detection strategy for AFP analysis. Investigations indicated that the 50 nm diameter GNS-labeled capture antibody can specifically catch AFPs by direct detection profile or by further signal amplification through AuNP-tagged enhance antibody combination. Results showed that the developed method holds 8.6 ng/mL sensitivity, 20.0–110.0 ng/mL detection range, acceptable precision and fine accuracy, as well as favorable specificity. Results of application to real serum determination by the proposed method are highly related to those of the ECLIA method (correlation coefficient is 0.931). The proposed method has simple-operation merit and is very suitable for clinical screening of large-scale serum samples of cancers.
研究领域:
医学诊断
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纳米生物学
·
临床检验
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2020
·
IF 9.038
Xinwen Cui,Tianxiang Wei,Mengyuan Hao,Qi Qi,Huafeng Wang,Zhihui Dai
In this work, we developed an electrochemical oxidation-assisted complexation strategy for highly sensitive and selective detection of thiocyanate (SCN − ). Gold nanostars (AuNSs) with uniform and sharp tips were first prepared, and we found they can be quickly etched to gold nanoparticles (AuNPs) under electrochemical oxidation with the existence of halide and halogen-like ions. Through introducing SCN − -selective molecule: zinc phthalocyanine (ZnPc), the fabricated ZnPc-AuNSs/ITO electrode can rapidly and selectively response to SCN − under electrochemical oxidation, manifesting as a noticeable change in color from navy blue to red. Thus SCN − concentration can be easily reflected. The wide wavelength tuning range of AuNSs to AuNPs make the ZnPc-AuNSs/ITO sensor obtain a much wider detection range for SCN − (10 nM to 80 mM) than most other reported studies. In addition, the detection limit is as low as 3 nM. It renders the sensor to be easily used in much diluted matrixes, which can further lower the interference. We further applied the colorimetric sensor to SCN − detection in wastewater and milk, excellent performance was obtained. The proposed electrochemical oxidation-assisted complexation strategy will have good promise in developing colorimetric sensors with high selectivity and wide detection range, and will display more useful application in environmental monitoring.
研究领域:
分析化学
·
环境科学
·
材料科学
✓ 已验证
DOI
PubMed
高影响因子
Pharmaceutics
·
2019
·Vol. 11
·期 12
·683
·
IF 4.773
Hongyan Zhu,Weili Han,Ye Gan,Qiaofeng Li,Xiaolan Li,Lanlan Shao,Dan Zhu,Hongwei Guo
In this study, we prepared gold nanostar (GNS) composite nanoparticles containing siRNA of cyclooxygenase-2(siCOX-2) that were modified by tumor targeting ligand 2-deoxyglucose (DG) and transmembrane peptide 9-poly-D-arginine (9R) to form siCOX-2(9R/DG-GNS). Paclitaxel loaded temperature sensitive liposomes (PTX-TSL) were surface-modified to produce PTX-TSL-siCOX-2(9R/DG-GNS) displaying homogeneous star-shaped structures of suitable size (293.93 nm ± 3.21) and zeta potentials (2.47 mV ± 0.22). PTX-TSL-siCOX-2(9R/DG-GNS) had a high thermal conversion efficiency under 808 nm laser radiation and a superior transfection efficiency, which may be related to the targeting effects of DG and increased heat induced membrane permeability. COX-2 expression in HepG2/PTX cells was significantly suppressed by PTX-TSL-siCOX-2(9R/DG-GNS) in high temperatures. The co-delivery system inhibited drug-resistant cell growth rates by ≥77% and increased the cell apoptosis rate about 47% at elevated temperatures. PTX-TSL and siCOX-2 loaded gold nanostar particles, therefore, show promise for overcoming tumor resistance.Keywords:gold nanostars;temperature sensitive liposome;siCOX-2;paclitaxel-resistance;hepatic carcinoma
研究领域:
纳米技术
·
肿瘤学
·
药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）