Toxics
·
2022
·Vol. 10
·期 6
·276
·
IF 4.472
Chusheng Liu,Yanjing Wang,Gaofei Zhang,Xuebin Pang,Jiao Yan,Xiaoou Wu,Yingheng Qiu,Ping Wang,Houshuang Huang,Xiaowei Wang,Haiyuan Zhang
Gold nanomaterials (Au NMs) have been widely used in cosmetic products for improving the brightening, and reducing the wrinkling of, skin, etc.; however, the dermal safety of Au NMs is rarely concerned. A previous study found that cosmetics could enhance the toxicity of Au nanosheets, but different physicochemical properties of Au NMs will induce different interaction modes with ingredients of cosmetics, potentially leading to different toxicity profiles. In the present study, spherical and rodlike Au NMs were first found in commercial cosmetics, and then Au nanospheres (NSs) with different sizes and Au nanorods (NRs) with different aspect ratios were prepared to simulate these Au NMs in cosmetics and further investigate their toxicity before and after embedment in cosmetics. It was found that the primary sizes, morphologies, and optical absorptions of these Au NSs and NRs before and after embedment were similar; however, their hydrodynamic sizes and zeta potentials were noticeably different. Then, these Au NSs and NRs presented weak or no cytotoxicity against HaCaT keratinocytes, while cosmetic cream could alleviate their cytotoxicity. Moreover, the cream could enhance the accumulation of Au NSs and NRs in the skin of hairless mice, but it also alleviated the toxicological responses of Au NSs and NRs in terms of superoxide dismutase (SOD) elevation and malondialdehyde (MDA) reduction. Therefore, the embedment of Au NSs and NRs into cosmetics can alleviate the in vitro and in vivo dermal toxicities of Au NSs and NRs.Keywords:gold nanomaterials;cosmetics;toxicity reduction;skin
研究领域:
生物学
·
医学
·
毒理学
✓ 已验证
DOI
PubMed
高影响因子
ACS Applied Bio Materials
·
2020
·
IF 0
Ruohao Zhang,Jie Yu,Kun Ma,Yufan Ma,Zhuo Wang
With the increasing threat of bacterial infection to human health, the development of different antimicrobial agents is essential. Therefore, based on the photothermal conversion properties of gold nanomaterials, the polyelectrolyte (PE)-coated gold nanorods (GNR@PE) and gold nanostars (GNS@PE) are designed and synthesized. Consequently, the chemo-photothermal synergistic antibacterial effect is achieved. GNR@PE effectively eliminates the high toxicity of cetyltrimethylammonium bromide (CTAB), and both GNR@PE and GNS@PE have good biocompatibility and stability. Because of the cation coating, GNR@PE and GNS@PE show high localized surface charge, which causes strong affinity to bacteria and destruction of bacterial cell walls and cell membranes. They have good chemical antibacterial effects, and the chemical antibacterial rates are above 50%. Under the irradiation of an 808 nm laser, for Gram-negative bacteria and Gram-positive bacteria, GNR@PE (50.00 μg/mL) and GNS@PE (55.00 μg/mL) can kill more than 99% of bacteria through chemo-photothermal effects. GNR@PE and GNS@PE can help eliminate inflammation caused by infection and promote wound healing in the mice model and have few side effects on the organs of mice.
研究领域:
生物医学工程
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纳米医学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
BIOINORGANIC CHEMISTRY AND APPLICATIONS
·
2023
·
IF 3.8
P. Baby Shakila,Abdurahman Hajinur Hirad,Abdullah A. Alarfaj,Samer Hasan Hussein-Al-Ali,Beza Mulugeta
Multiple chemodrugs with nanotechnology have proven to be an effective cancer treatment technique. When taken combined, cabazitaxel (CTX) and cisplatin (PT) have more excellent cytotoxic effects than drugs used alone in the chemotherapy of several different cancers. However, several severe side effects are associated with using these chemotherapy drugs in cancer patients. Gold nanomaterials (AuNMs) are promising as drug carriers because of their small diameter, easy surface modifications, good biocompatibility, and strong cell penetration. This work aimed to determine the CTX and PT encapsulated with AuNMs against human glioma U87 cancer cells. The fabrication of the AuNMs achieved a negative surface charge, polydispersity index, and the mean sizes. The combined cytotoxic effect of CTX and PT bound to AuNMs was greater than that of either drug alone when tested on U87 cells. The half inhibitory concentration (IC50) values for free PT were 54.7 μg/mL (at 24 h) and 4.8 g μg/mL (at 72 h). Results acquired from the MTT assay show cell growth decreases time- and concentration-dependent AuNMs, free CTX, free PT, and AuNMs@CTX/PT-induced cytotoxicity and, ultimately, the cell death of U87 cells via apoptosis. The biochemical apoptosis staining techniques investigated the cells’ morphological changes of the cells (acridine orange and ethidium bromide (AO-EB) and nuclear staining (DAPI) techniques). The AO-EB and nuclear staining results reveal that the NPs effectively killed cancer cells. Furthermore, the flow cytometry analysis examined the mode of cell death. Therefore, AuNMs@CTX/PT has excellent potential in the cancer therapy of different cancer cells.
研究领域:
肿瘤学
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纳米医学
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药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Applied Sciences-Basel
·
2019
·Vol. 9
·期 5
·935
·
IF 2.217
Jing Neng,Chen Xiang,Kan Jia,Xiaohua Nie,Peilong Sun
Featured ApplicationThe prepared hydrophobic gold nanomaterials show excellent surface-enhanced Raman scattering activity and, therefore, could be used as efficient organic-soluble SERS substrates for the detection of many hydrophobic food toxicants, such as 3,4-benzopyrene, and carcinogens, such as benzidine.AbstractMany previously reported syntheses of gold nanoparticles required lengthy reaction times, complicated operations, high temperatures, or multi-step manipulations. In this work, a morphology-controlled versatile one-pot synthesis of hydrophobic gold nanodots, nanobars, nanorods, and nanowires has been developed. A series of gold nanomaterials ranging from round nanodots, short nanobars, and long nanorods to ultrathin and ultralong nanowires (diameter <2 nm, length >2 μm) have been readily prepared by simply adjusting the feeding ratio of chloroauric acid to oleylamine, oleic acid, and triphenylsilane. The silk-like ultralong and ultrathin nanowires were found to have a single crystalline structure and may have significant potential applications in microelectronics and biosensors. Large sizes of gold spherical nanoparticles were obtained from gold nanodots via a seed-mediated growth approach. These nanoparticles and ultralong nanowires showed excellent surface-enhanced Raman scattering (SERS) activity in organic solvents and, therefore, were employed as efficient organic-soluble SERS substrates for the detection of hydrophobic food toxicants, such as 3,4-benzopyrene, and carcinogens, such as benzidine.Keywords:synthesis;gold nanodots;nanobars;nanorods;nanowires;surface-enhanced Raman spectroscopy
研究领域:
纳米技术
·
分析化学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
·
2017
·
IF 3.582
Qiannan Wang,Hongyu Yang,Zhijun Zhou,Likai Wang,Wei Yan,Wen Wu,Shaowei Chen,Zhen Liu,Zhenghua Tang
Peptide based methods create new revenues to fabricate stable, multifunctional noble metal nanomaterials under mild and green synthesis conditions. Peptide sequence A4 stabilized AuAg alloyed nanomaterials were fabricated through a facile and straightforward approach. The as-prepared alloyed nanomaterials demonstrated effective catalytic activity toward oxygen electroreduction in alkaline media . Both the metal-to-A4 ratio and Au-to-Ag ratio were optimized to enrich the electrocatalytic performance, and the sample of Au:Ag:A4 = 10:30:1 exhibited the highest activity among the series. The performance of Au:Ag:A4 = 10:30:1 is comparable with that of commercial Pt/C, within the context of onset potential, diffusion-limited current density as well as long-term stability. The as-prepared samples were characterized by UV–visible absorbance, transmission electron microscopy (TEM), as well as X-ray photoelectron microscopy (XPS). The improved activity is probably attributed to the alloying induced ensemble effects and electronic effects. This discovery may shed light on fabricating peptide based alloys as highly efficient catalysts with enhanced stability for oxygen reduction reaction (ORR).
研究领域:
生物化学
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纳米材料科学
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电化学
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2018
·
IF 5.123
Min-Fu Wu,Yu Wang,Sha Li,Xiu-Xiu Dong,Jin-Yi Yang,Yu-Dong Shen,Hong Wang,Yuan-Ming Sun,Hong-Tao Lei,Zhen-Lin Xu
An electrochemical immunosensor for ultrasensitive detection of acrylamide (AA) in water and food samples was developed. SnO 2 -SiC hollow sphere nanochains with high surface area and gold nanoparticles with good electroconductivity were fabricated onto the surface of a glassy carbon electrode pre-coated with chitosan. The coating antigen (AA-4-mercaptophenylacetic acid-ovalbumin conjugate , AA-4-MPA-OVA) was immobilized on the electrode. Polyclonal antibody specific for AA-4-MPA was conjugated to gold nanorod (AuNR) as primary antibody (AuNR-Ab 1 ). Horseradish peroxidase labelled anti-rabbit antibody produced in goat was conjugated to AuNR as secondary antibody (HRP-AuNR-Ab 2 ). For detection, the analyte (AA-4-MPA) in sample competed with coating antigen for binding with AuNR-Ab 1 . After washing, HRP-AuNR-Ab 2 was added to capture the AuNR-Ab 1 , and the electrical signal was obtained by addition of hydroquinone and H 2 O 2 . After investigation of the binding ability on nanomaterials and optimization of competitive immunoassay conditions, the proposed immunosensor exhibited a sensitive response to AA with a detection limit of 45.9 ± 2.7 ng kg −1 , and working range of 187 ± 12.3 ng kg −1 to 104 ± 8.2 μg kg −1 for drinking water samples. Recoveries of AA from spiked samples were ranged from 86.0% to 115.0%. The specificity, repeatability and stability of the immunosensor were also proved to be acceptable, indicating its potential application in AA monitoring.
研究领域:
食品科学
·
分析化学
·
生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
International Journal of Electrochemical Science
·
2023
·
IF 1.5
Zhenggui Tao,Jinghu Du,Yu Cheng,Qingfeng Li
In the present work, carcinoembryonic antigen (CEA), a significant biomarker in gastric cancer diagnostics, was successfully detected by a desirably selective and sensitive electrochemical aptasensor with an electrocatalyst and nanocarrier, i.e., Pt/Au-diaminonaphthalene (DN)-graphene. The developed bioconjugate was captured onto the surface of the electrode via a “sandwich” strategy during the detection of CEA. The proposed method was demonstrated to be sensitive, as indicated by the improved electrochemical response, since the dendritic Pt/Au/DN-graphene showed peroxidase-mimic activity for the reduction of H 2 O 2 introduced into the electrolytic cell, thereby confirming its desirable catalysis capacity.
研究领域:
生物医学工程
·
肿瘤学
·
分析化学
✓ 已验证
DOI
高影响因子
ANALYTICAL LETTERS
·
2024
·
IF 1.6
Zehui Shi,Hongli Zhao,Fangfang Zhou,Yi Mao,Zheng Gong,Minbo Lan
Serum homocysteine (Hcy) is considered to be a reliable biomarker of cardiovascular, cerebrovascular and neurological diseases. Therefore, rapid, specific and sensitive measurement of homocysteine is crucial for prevention and diagnosis. Electrochemical methods, which benefit from simplicity, low-cost and quick response, have been widely utilized in the determination of targeted biomolecules. In this work, based upon a portable mini-potentiostat, a homocysteine electrochemical biosensor has been successfully developed combined with the modification of gold and manganese (IV) nanomaterials on multiwalled carbon nanotubes (MWCNTs) (denoted as Au@MnO2/MWCNTs) on screen-printed electrodes (SPEs). Due to the excellent electrochemical activity and catalytic capability of Au@MnO2/MWCNTs, the proposed electrochemical biosensor exhibited wide-linear range (5–125 μmol L−1), high sensitivity (0.01110 μA (μmol L−1)−1) and low detection limit (0.6173 μmol L−1) for homocysteine, requiring only 400 s to complete detection. It was effectively applied in the determination of serum homocysteine, and the results were consistent with those of an automated clinical chemistry analyzer. Furthermore, the simple and portable electrochemical device offers a promising manner for on-site analysis and home health monitoring.
研究领域:
生物传感技术
·
医学诊断
·
纳米材料科学
✓ 已验证
产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2022
·
IF 5.304
Zhengzheng Wang,Xiaoyan Feng,Fangbin Xiao,Xuekun Bai,Qian Xu,Hengyi Xu
Staphylococcus aureus ( S. aureus ) is one of the pathogens that seriously endanger human health and are widespread in nature. Food poisoning incidents caused by S. aureus frequently break out. Therefore, it is significant that a rapid and accurate method is established for S. aureus detection. In this study, a strategy using magnetic separation combine with fluorescence analysis was proposed for S. aureus detection. Boric acid can selectively enrich compounds with cis -diol groups through a reversible esterification reaction, and has been widely used in the identification of sugars and glycosylated molecules. As a boric acid derivative, 3-aminophenylboronic acid (APBA) has the advantages of stability, low cost, easy synthesis, etc. Therefore, using APBA as a recognition molecule to prepare magnetic nanomaterials can achieve the enrichment of S. aureus . In order to reduce steric hindrance and ensure the dominant orientation of APBA, thus improving the enrichment performance of target bacteria. The polyethylene glycol (PEG) was used as a mediator to prepare PEG-mediated APBA functionalized magnetic nanomaterials (APBA-PEG-MNs). In addition, this study used FITC-pig IgG to label S. aureus because the Fc fragment of IgG to bind protein A on the surface of S. aureus and realized the detection of S. aureus by detecting fluorescence. Under the optimal experimental conditions, the capture efficiency of APBA-PEG-MNs for S. aureus with the concentration of 2.7 × 10 6 CFU/mL in spinach, juice and pool water spiked samples was 92.6%, 91.6%, 91.6%. The limit of detection of the strategy in spiked samples was 2.7 × 10 2 CFU/mL within 80 min.
研究领域:
生物化学
·
分析化学
·
食品科学
✓ 已验证
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2023
·
IF 4.4
Hongyan Xi,Ziqian Shi,Pengfei Wu,Niu Pan,Tingting You,Yukun Gao,Penggang Yin
Antioxidants play an important role in life health and food safety. Herein, an inverse-etching platform based on gold nanorods (AuNRs) and gold nanostars (AuNSs) for high-throughput discrimination of antioxidants was constructed. Under the action of hydrogen peroxide (H 2 O 2 ) and horseradish peroxidase (HRP), 3,3′,5,5′-tetramethylbenzidine (TMB) would be oxidized to TMB + or TMB 2+ . HRP reacts with H 2 O 2 to release oxygen free radicals, which then react with TMB. Au nanomaterials can react with TMB 2+ , at the same time, Au was oxidized into Au (I), leading to the etching of the shape. Antioxidants, with good reduction ability, would prevent the further oxidation of TMB + to TMB 2+ . So the presence of antioxidants will prevent further oxidation while avoiding the etching of Au in the catalytic oxidation process, thereby achieved inverse etching. Distinctive surface enhanced Raman scattering (SERS) fingerprint of five antioxidants were obtained based on the differential ability to scavenge free radicals. Five antioxidants, including ascorbic acid (AA), melatonin (Mel), glutathione (GSH), tea polyphenols (TPP), and uric acid (UA) were successfully distinguished by using linear discriminant analysis (LDA), heat map analysis and hierarchical cluster analysis (HCA). The study exhibits an effective inverse-etching based SERS sensor array for the response of antioxidants, which has great reference value in the field of human disease and food detection.
研究领域:
生物化学
·
分析化学
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nanoscience and Nanotechnology Letters
·
2020
·Vol. 12
·期 9
·1079-1086
·
IF 1.128
Hao Haitao
At present, chemical biosensors still have poor reproducibility and stability due to that the density of nanomaterials particles is not ideal. Therefore, an optimal design method of electrochemical biosensor based on gold nanomaterials is proposed. Two types of sensors, namely DNA biosensor
and immune biosensor, were designed in this work. A DNA biosensor with gold nanorod nanocomposite modified electrode was prepared, and its electrical activity was measured by Methylene blue. In contrast, an electrochemical immune sensor with gold nanorod-modified electrode was prepared and
used for detection of alpha fetoprotein. The experimental results show that the designed DNA biosensor and electrochemical immune sensor have the advantages of simple preparation, high sensitivity, good selectivity and good stability.
研究领域:
生物传感技术
·
纳米生物学
·
医学检测
✓ 已验证
DOI
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2018
·
IF 5.091
Nannan Sun,Kaisheng Yao,Chao Wang,Chenchen Zhao,Weiwei Lu,Shuang Zhao,Huiyong Wang,Jianji Wang
Developing a simple and green route for the controlled synthesis of hierarchical nanomaterials with anisotropic particles as subunits is highly desirable and technically important. Here, a functionalized ionic liquid (FIL) assisted strategy is developed for the preparation of hierarchical Au nanomaterials at room temperature. Various morphologies of Au products, including nanoplate-built flowers, nanobar-built flowers and nanourchins, have been well achieved using the current route in aqueous solutions of imidazolium ILs with different functional groups, such as 1-carboxymethy-3-methylimidazolium chloride ([CMmim]Cl), 1-hydroxylethyl- 3-methylimidazolium chloride ([HEmim]Cl), and 1-(3-aminopropyl)-3- methylimidazolium chloride ([APmim]Cl). The influence of these FILs on the morphology of Au products and their growth process has been studied systematically. It is shown that the functionalized ILs play important roles in the construction of these interesting Au hierarchies. The seed, IL concentration (within a certain range), reaction temperature have negligible influence, but stirring and anions of the ILs have important influences, on the product morphologies. Based on these results, the possible growth mechanism is proposed for the formation of different Au hierarchies. In addition, all the Au samples prepared with the assistance of the functionalized ILs, especially the nanoplate-built flowers, exhibit striking SERS sensitivity and high reproducibility.
研究领域:
材料科学
·
纳米技术
·
化学合成
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）