BioNanoScience
·
2025
·Vol. 15
·期 2
·1-10
·
IF 3.2
Cai He-Qing,Han Lu,Xue Xin-Yu,Zhang Kou,Wang Tian-Hao,Pan Fu-Hong,Sun Zhi-Cheng,Liu Ru-Ping,Hu Kun,Wei Yen
Gold nanocages (AuNCs) exhibit tunable local surface plasmon resonance (LSPR) properties and exceptional drug loading and releasing capabilities, attributes stemming from their hollow interiors and ultra-thin porous walls. These features underscore their promising applications in the biomedical field. In this study, we fabricated AuNCs with LSPR peaks at 810 nm via chemical etching of Ag nanocube templates with sharp edges using a precisely controlled volume of chloroauric acid. To impart gene-loading functionality, we chemically anchored thiol-modified polyethyleneimine (PEI) onto the surfaces of AuNCs through robust Au–S bonds, generating a novel gene carrier, AuNCs-PEI. Subsequently, we evaluated its photothermal efficacy using a femtosecond laser and assessed cytotoxicity via MTT assay. The binding affinity of AuNCs-PEI for EGFP plasmids was characterized by gel retardation assays. Furthermore, the gene delivery efficiency of AuNCs-PEI was examined in 293 T cells by quantifying green fluorescence protein signals using inverted fluorescence microscopy. These findings demonstrate that engineered AuNCs-PEI not only facilitates gene loading for therapeutic applications but also enables photothermal therapy (PTT).
研究领域:
生物医学工程
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纳米医学
·
基因治疗
✓ 已验证
产品关联
DOI
高影响因子
Journal of Materials Chemistry B
·
2024
·
IF 6.1
Ran Hao,Meng Jiao,Xingguo Xu,Di Wu,Haiying Wei,Leyong Zeng
Delivery nanosystems were popularly developed to improve chemotherapy efficacy, but the non-specific leakage of drugs was still not satisfactory. Here, using gold nanocages (AuNCs) as carrier and thermo-sensitive liposome (TSL) as protection shell, a camptothecin (CPT) loaded delivery nanosystem (AuNCs/CPT@TSL) was designed for photothermal-modulated drug release, by which the non-specific leakage of CPT was avoided, and the combination of photothermal therapy (PTT) and chemotherapy was achieved. In the simulated tumor microenvironment (pH=5.5), the TSL shell protected CPT from leakage at 37℃, and the release rate of CPT was only 11.4%. However, the release rate of CPT greatly increased to 85.4% by elevating temperature to 45℃. The photothermal conversion efficiency of AuNCs/CPT@TSL reached up to 46.1%. When the incubation temperature was 37℃, the cell survival rate was reduced to 43.6% in AuNCs/CPT, but was still above 90% in AuNCs/CPT@TSL, demonstrating the good protection of TSL shell. Under the combination of PTT/chemotherapy, the cell viability greatly decreased to 10.9%, and the tumors completely disappeared, confirming the safe and reliable antitumor effect of AuNCs/CPT@TSL.
研究领域:
纳米医学
·
肿瘤学
·
药物递送
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2018
·Vol. 6
·期 15
·2217-2229
·
IF 4.776
Shengnan Huang,Ying Liu,Xin Xu,Mengfei Ji,Yuanmin Li,Chengjun Song,Shaofeng Duan,Yurong Hu
A combination of different therapy strategies has great potential to efficaciously treat malignant tumors, by virtue of their synergetic effects. Herein, a co-delivery system based on gold nanocages (AuNCs) was designed to deliver both doxorubicin (DOX) and microRNA-122 mimic (miR-122) for an enhanced cancer therapy. DOX was loaded into the AuNCs and miR-122 was condensed onto the surface of the functionalized AuNCs by an electrostatic interaction. Polyethyleneglycol (PEG) and hyaluronic acid (HA) were also introduced to the co-delivery system for targeted drug delivery. We evaluated the cellular uptake, biodistribution and anti-tumor effect in vitro and in vivo. Our results demonstrated an effective delivery of DOX and miR-122 into tumor cells and the tumor tissue. Importantly, the triple therapy, namely the combination of chemotherapy, gene therapy and photothermal therapy, mediated by this multifunctional drug delivery system, exhibited better anti-tumor effect than any single therapy, both in vitro and in vivo. Additionally, this drug delivery system caused insignificant toxicity to the major organs and had no obvious effect on the body weight of the mice. It could be concluded that multifunctional AuNCs are promising as a co-delivery vector for an enhanced anti-tumor effect.
研究领域:
肿瘤学
·
纳米医学
·
基因治疗
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nanoscale
·
2018
·Vol. 10
·期 42
·20020-20032
·
IF 7.233
Jiangshan Wan,Shinan Geng,Hao Zhao,Xiaole Peng,Jiabao Xu,Meihe Wei,Junxiong Mao,Yang Zhou,Quan Zhu,Yanbing Zhao,Xiangliang Yang
Though a therapeutic sequence plays a key role in tumor therapy, little attention has been paid to its influence on multimodal combined therapy. Herein, we developed gold nanocages (GNC@PNA-hls) decorated with two kinds of temperature sensitive p(N-isopropyl-acrylamide-acrylic acid) copolymers (PNA-hs and PNA-ls) for precise antitumor coordination of thermo-chemotherapy. Doxorubicin-loaded GNC@PNA-hls (Dox-GNC@PNA-hls) showed a steady photothermally induced on-demand release under multiple near-infrared (NIR) irradiations. In vitro evaluations indicated that concurrent thermo-chemotherapy treatments (Dox − L) showed the best antitumor effect, compared with the sequence of either doxorubicin treatment followed by NIR radiation (Dox + L) or NIR radiation followed by doxorubicin treatment (L + Dox). The in vivo antitumor efficacy also indicated that the tumor volume was totally suppressed (ca. 0.14 cm3) by the treatment of Dox-GNC@PNA-hls with NIR radiation for 14 days. These results indicated that Dox-GNC@PNA-hls could achieve precise synchronization between hyperthermia and chemotherapy, and effectively enhance their antitumor efficacy.
研究领域:
肿瘤学
·
纳米医学
·
生物材料学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2025
·
IF 6.1
Jin Wang,Xinyu Lu,Huafeng Wang,Yuan Zhong,Zhihui Dai,Tianxiang Wei
In this work, Ru(bpy) 3 2+ -loaded gold nanocage (AuNCs) (Ru–AuNCs) was prepared and found to display a distinct property of electrochemiluminescence (ECL) enhancement under mercury ions (Hg 2+ ) interaction. Based on this, we designed a screen-printed bipolar electrode-ECL (SPBPE-ECL) sensing platform by coupling with the thymine-Hg 2+ -thymine (T-Hg 2+ -T) binding pattern for one-step highly sensitive detection of Hg 2+ . This ECL sensor showed a wide linear detection range (0.75 – 850 μg L −1 ) and low detection limit (0.1290 μg L −1 ) toward Hg 2+ , with a one-step detection procedure and disposable feature, displaying potential applicability in the point-of-care-testing (POCT) of Hg 2+ in the environment. In addition, the Hg 2+ -mediated ECL signal enhancement mechanism of Ru–AuNCs was also investigated. It was confirmed that Hg 2+ interaction etched the cage structure of Ru–AuNCs, which sped up the release of more Ru(bpy) 3 2+ around the sensing electrode. Furthermore, Au–Hg alloy structure was formed on the surface of Ru–AuNCs, which also improved the ECL signal. This target-induced in-situ sensing material surface reconstruction strategy would provide a better design concept for the construction of ECL POCT sensor.
研究领域:
环境科学
·
分析化学
·
生物传感
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产品关联
DOI
PubMed
高影响因子
Biomimetics
·
2025
·Vol. 10
·期 7
·430
·
IF 3.9
Xin Jin,Hu Qian,Yuxiang Xie,Changzhi Liu,Yuan Cheng,Jinsong Hou,Jiandong Zheng
Despite the promising natural origin, biocompatibility, and biodegradability of chitosan for biomedical applications, developing biodegradable nanocarriers with controllable sizes and precise drug delivery targeting remains a significant challenge, hindering its integration into precision medicine. To address this, we synthesized gold nanocage (AuNCs)/poly-(N-isopropylacrylamide-co-carboxymethyl chitosan) core-shell multifunctional composite nanospheres (CPAu) through a two-step one-pot method. The resulting CPAu nanospheres (~146 nm in size) exhibited multi-sensitive release properties, excellent biocompatibility, and potent photothermal therapy (PTT) activity. These nanospheres effectively encapsulated diverse antitumor drugs while demonstrating triple responsiveness (thermo-, reduction-, and PTT-triggered) for targeted tumor cell delivery, thereby achieving enhanced antitumor efficacy in combinatorial chemotherapy.
研究领域:
生物材料
·
纳米医学
·
肿瘤学
·
药剂学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nanoscale
·
2017
·Vol. 9
·期 21
·7284-7296
·
IF 7.367
Shijin Bao,Shengnan Huang,Ying Liu,Yurong Hu,Weiping Wang,Mengfei Ji,Huili Li,Ning Xia Zhang,Chengzhi Song,Shaofeng Duan
Numerous studies have demonstrated that microRNAs are very important in cancer development and progression. However, the complex relationship between the size of microRNA delivery systems, cellular uptake, biodistribution and therapeutic efficiency remains unclear. Herein, we have successfully constructed a series of differently-sized microRNA delivery systems, miR-26a-loaded, hyaluronic acid-modified, polyetherimide-conjugated PEGylated gold nanocage ternary nanocomplexes (PPHAuNCs-TNCs), which can be monitored optically by fluorescence and photoacoustic tomography imaging. We evaluated the effect of the particle size on the cellular uptake and biodistribution in the BEL-7402 cell line in vitro and in the subcutaneous and orthotopic hepatocellular carcinoma (HCC) mouse models. Our findings showed that the cellular uptake and biodistribution were optimal for cancer therapy with the PPHAuNCs-30-TNCs (30 nm AuNCs in edge length) in comparison with their 50 nm and 70 nm counterparts. PPHAuNCs-30-TNCs could accumulate in the liver for a longer time in an orthotopic mouse model of HCC than that in normal mice and could considerably restrain tumor growth in an orthotopic HCC mouse model under near-infrared radiation. This study may provide insightful information for developing novel non-viral microRNA vectors, and PPHAuNCs-30-TNCs have great potential for application in tumor diagnosis and cancer therapy in the future.
研究领域:
分子生物学
·
纳米医学
·
肿瘤学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF ALLOYS AND COMPOUNDS
·
2021
·
IF 5.316
Jinghua Li,Jianbo Zhang,Xibin Jiao,Jiahua Shi,Jiangxue Ye,Kena Song,Jianfeng Bao,Guangda Li,Kun Lei
In this work, near-infrared (NIR) light driven concave octopod PtCu alloy nanocages (OPtCu-NCs) were fabricated and first developed as photothermal synergetic Fenton catalyst for degradation of pollutant dyes. Moreover, by irradiating with NIR laser (808 nm), the obtained OPtCu-NCs exhibit excellent NIR absorption capacity and thus produce hyperpyrexia over 50 °C. Concomitantly, by the photothermal effect, the OPtCu-NCs nanocatalysts was used for elimination of organic dyes such as Methylene blue (MB) and Rhodamine B (RhB). The NIR-driven photothermal degradation mechanism under NIR irradiation can be attributed to the Fenton-like catalytic performance of Cu ions , then -N C- and/or C-C bonds in the molecular skeleton were fractured and electrons were donated intensively. As confirmed by a typical radical trapping experiment, electrons trigger the formation of miscellaneous reactive species (O 2 - , HO 2 , especially •OH) subsequently . Moreover, the photothermal Fenton-catalytic activity of the as-prepared OPtCu-NCs had maintained at 93.15% (for MB) and 91.87% (for RhB) after ten circulating degradation processes. All acquired results reveal that the OPtCu-NCs have the potential applied value in dyestuff-wastewater treatment under neutral pH.
研究领域:
环境科学
·
纳米材料
·
催化化学
✓ 已验证
产品关联
DOI
高影响因子
ANALYST
·
2015
·Vol. 140
·期 5
·1672-1677
·
IF 4.107
Wei Wang,Na Zhao,Xiaoxiao Li,Jun Wan,Xiliang Luo
A novel controlled-release biosensor for isothermal amplified detection of ATP using Au nanocages (AuNCs) capped with a DNA molecular gate is reported for the first time, and has been successfully tested in intracellular ATP detection. Two kinds of SH-modified short strand DNAs S1 and S2 were assembled on the surface of the AuNCs by means of Au–thiolate bonding. The hybridization of a long-strand DNA S3 with the two immobilized SH-DNAs leads to the formation of molecular gates. The molecular gates were designed to inhibit the release of the fluorescent molecules such as Rhodamine-B (RhB), which were filled in the hollow interiors of AuNCs. The primer S4 was employed to play the role of a recognition moiety. The specificity recognition reaction between ATP and ATP aptamer gave rise to the primer S4 released from a double-stranded hybrid formed with the ATP aptamer. The released S4 will initiate the autonomous replication–scission–displacement process with the assistance of DNA polymerase and nicking endonuclease. As a result, the DNA synthesis and the DNA cycle achieved the opening of the DNA-based molecular gates and the significant amplification of the release of the guest molecules from AuNCs. In order to realize the cyclic enzymatic amplification of the release of the guest molecules from AuNCs, the long-strand S3 is ingeniously designed in such a way that it contains a Nb.Bpu10I nicking endonuclease recognition sequence and a sequence complementary to the primer S4. The fabricated system was demonstrated to be an efficient biosensor for target molecule detection qualitatively and quantitatively.
研究领域:
生物传感
·
纳米技术
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
International Journal of Nanomedicine
·
2017
·
IF 4.3
Shengnan Huang,Chunming Li,Weiping Wang,Huanjie Li,Zhi Sun,Chengzhi Song,Benyi Li,Shaofeng Duan,Yurong Hu
The combination of photothermal therapy and chemotherapy (photothermal–chemotherapy) is a promising strategy for cancer therapy. Gold nanocages (AuNCs), with hollow and porous structures and unique optical properties, have become a rising star in the field of drug delivery. Here, we designed a novel targeted drug delivery system based on functionalized AuNCs and evaluated their therapeutic effects in vitro and in vivo. We then loaded doxorubicin into this promising system, designated as DHTPAuNCs consisting of hyaluronic acid-grafted and A54 peptide-targeted PEGylated AuNCs. Its formation was corroborated by ultraviolet–visible spectroscopy, transmission electron microscopy and dynamic light scattering. This delivery platform needed hyaluronidase to release encapsulated drugs, meanwhile the acidic pH and near-infrared irradiation could accelerate the release. In addition, the results of cellular uptake demonstrate that this system could bind specifically with BEL-7402 cells. In vitro, we evaluated therapeutic effects of the DHTPAuNCs in BEL-7402 cells by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl-tetrazolium bromide assay. Moreover, in BEL-7402 tumor-bearing nude mice, its therapy effect in vivo was also evaluated. As expected, DHTPAuNCs exhibited excellent therapeutic effect by photothermal–chemotherapy, both in vitro and in vivo. In short, DHTPAuNCs with low toxicity showed great potential as a drug delivery system for cancer therapy.
研究领域:
纳米技术
·
癌症治疗
·
药物递送系统
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2020
·
IF 3.721
Zhao Tan,Wenqin Wu,Nanri Yin,Mingming Jia,Xiaomei Chen,Yizhen Bai,Huimin Wu,Zhaowei Zhang,Peiwu Li
Selenium is one of the most attractive nutrient elements for antioxidant, anticancer and immunomodulatory effects, which is obtained from the agricultural food and environment. Herein, a gold nanocages/fluorinated graphene (AuNCs/FG) nanocomposite modified glassy carbon electrode (GCE) was fabricated for the electrochemical determination of selenium by square wave anodic stripping voltammetry. Under the optimal parameters, a wide linear relationship between peak current of selenium on AuNCs/FG/GCE and selenium concentration was observed between 0.002−5.0 mg/L. The method exhibited a low detection limit of 0.27 μg/L. The proposed method was applied successfully for the determination of selenium in agricultural food (tea, rapeseed sprouts, peanut) and environmental (tap water and lake water) samples. The results showed that the average levels of selenium were 0.04−0.27 mg/kg and not detectable, in agricultural food and water, respectively. Therefore, this method has great potential for the monitor of selenium in food and environmental samples.
研究领域:
分析化学
·
营养学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
Biomaterials Science
·
2021
·Vol. 10
·期 8
·1995-2005
·
IF 6.843
Yun Qiu,Shimin Yu,Yulan Wang,Leyi Xiao,Linsen Pei,Yingying Pu,Yufeng Zhang
Photothermal nanoparticles are thought to be the most suitable candidates against infectious disease by disrupting the cell membrane or inhibiting cellular metabolism. However, cells with low-metabolic activity states may be endowed with greater ability against harsh environments including antibiotic treatment. For now, it remains unexplored whether and how photothermal therapy (PTT) gives rise to bacterial antibiotic tolerance. In this study, we showed that although it exhibits excellent bactericidal ability, PTT with typical photothermal nanoparticle gold nanocages (AuNCs) can give rise to a subpopulation of cells with great ability of antibiotic tolerance. The subpopulation exhibits delayed growth and decreased cellular ATP levels, indicating a low metabolic state. Specifically, after AuNCs attach to the surface of a bacterial cell, photothermal manipulation can induce cell membrane shrinkage and block the bacterial respiratory chain. Besides, heat shock induces protein aggregation and leads to the dysfunction of a number of important proteins. The heat shock protein DnaK is closely associated with protein aggregation and plays a vital role in modulating antibiotic tolerance, providing a potential therapeutic target.
研究领域:
微生物学
·
纳米医学
·
感染病学
✓ 已验证
DOI
PubMed
高影响因子
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