GIANT
·
2020
·
IF 0
Xinyi Jin,Jinqiao Xue,Dong Hee Kang,Yujing Liu,Jie Ren,Quan Wen,Dapeng Hu,Yan Yu,Wei Yang,Binyang Du,Myung-Han Yoon,Hanying Li
Single crystals have been shown to incorporate guest materials (e.g., gel networks and functional particles) and the incorporated materials endow the crystal hosts with non-intrinsic properties. The acquisition of the non-intrinsic crystal properties impels us to diversify the incorporated guest materials and thus to spread the potential applications of single-crystalline materials. Here, fluorescent NIPAM-based microgels have been selected as the guest materials to be incorporated into the crystal hosts. In the presence of microgels, calcite crystallize from either solution or gel media have been examined. Solution-grown crystals exclude the microgels, while two gel-grown methods have proved effective for incorporating these microgels inside calcite single crystals. On one hand, an additional macroscopic agarose gel-network is introduced to trap the microgels. Crystallization in the agarose gel leads to incorporation of agarose gel-network together with the trapped microgels. The microgel concentration in the crystal reaches 33 mg/mL. On the other hand, dispersions of microgels are concentrated to about 352 mg/mL and the microgels aggregate into non-flowing macroscopic gels (termed as macrogels). Crystallization in a macrogel results in its incorporation. By showing the incorporation of microgels through physical entrapment, this work provides a new platform to functionalize single crystals by the microgels.
研究领域:
材料科学
·
化学
✓ 已验证
DOI
Biomaterials Science
·
2025
·
IF 5.7
Xue-Wu Chen,Jun Long,Quan Zhang,Linghong Huang,Xin-Yuan Sun
s: Background: Calcium oxalate (CaOx) crystal deposition and its resultant cellular oxidative damage and inflammation are important causes of renal stone formation. It is clinically important to conduct research on multifunctional anti-stone drugs targeting these predisposing factors. Methods: We modified natural Undaria pinnatifida polysaccharide (UPP0) by sulfation via the sulfur trioxide-pyridine method, resulting in four sulfated polysaccharides with varying sulfate group (–OSO3–) contents: 1.59% (UPP0), 6.03% (UPP1), 20.83% (UPP2), and 36.39% (UPP3), and compared their differences in the inhibition of crystalline formation, renal injury, and inflammation in the process of renal stone formation at chemical and cellular levels. Results: The UPPS were able to inhibit the nucleation, growth and aggregation of CaOx crystals in vitro. Among them, UPP3 with the maximum sulfate group content showed the greatest crystallization inhibition ability. The nucleation inhibition and aggregation inhibition of UPP3 at a concentration of 0.5 mg/mL were as high as 80.21% and 72.34%, respectively. The CaOx crystal size regulated by UPP3 was significantly reduced from 25.9 ± 2.8 μm to 5.9 ± 1.2 μm. Furthermore, UPPS were observed to up-regulate the expression of the antioxidant enzyme superoxide dismutase (SOD) in cells, reduce the levels of ROS and malonaldehyde (MDA), enhance lysosomal integrity, decrease intracellular Ca2+ levels, inhibit the decline in mitochondrial membrane potential, reduce the production of cellular inflammatory factors (TNF-α, MCP-1, IL-18, and IL-1β), and ultimately inhibit cell apoptosis. Conclusion: UPPS combine multiple biological functions of crystallization regulation, antioxidant and anti-inflammatory, and have important potential in the prevention of kidney stones. Sulfation modification can improve the biological activity of UPP0 and provide a reference for screening and optimization methods of stone drugs.
研究领域:
医学
·
药理学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
APPLIED SURFACE SCIENCE
·
2022
·
IF 7.392
Si Li,Xiang Kang,Qing He,Weiwei Tang,Junbo Gong
Designing (macro)molecules controlling crystallization is an essential approach to regulate structure and properties of crystalline materials and circumvent detrimental mineralization in nature, biological, and synthetic systems. Here we discovered four novel, natural polyphenols, including gallic acid, ellagic acid, punicalagin, and tannic acid, as a new class of inhibitors for nucleation and crystal growth of calcium oxalate (CaOx), a primary constitution of kidney stones. Study of time-resolved growth reveals that polyphenols regulate the kinetics of crystal-liquid interface acting as a dual role of growth promoter and inhibitor which displays a new record of growth promotion up to 75% or complete suppression, dependent upon the modifier concentration. Time-elapsed in situ atomic force microscopy measurements unveil these modifiers regulate the growth mode from spiral-mediated to generation of two-dimensional (2D) islands and layer spreading mechanism, leading to formation of step bunches growing out of (0 1 0) surface and forming macroscopically crystal whiskers. The governing regime between growth promotion and inhibition is determined by inhibitors concentration but can be influenced by crystallization driving force. Such unique growth mechanism of crystal whiskers may also provide potential implications for understanding and synthesizing branched and hierarchical structures of biomimetic materials.
研究领域:
生物化学
·
材料科学
·
医学
✓ 已验证
DOI
高影响因子
AICHE JOURNAL
·
2024
·Vol. 70
·期 8
·e18474
·
IF 3.5
Yuchao Niu,Shaofu Du,Shankun Liang,Lei Sheng,Yingshuang Meng,Wu Xiao,Xuehua Ruan,Gaohong He,Xiaobin Jiang
Confined crystallization can realize controllable nucleation and growth of crystals with targeted morphologies in a restricted region. Here, we achieved confined reactive crystallization within a novel micro columnar jet flow regime constructed by the hollow fiber membrane. The interface of the jet flow corresponds as the restricted region for reactive crystallization, and the large-sized calcium carbonate (CaCO 3 ) crystals aggregates with millimeter length can ordered and self-assembly grow along the boundary of this region. Formation mechanism of the jet flow was systematically investigated by real-time observation and force analysis. The detected detachment-regrowth cycle during the experiments furtherly uncovered the potential for continuous operation. Linear (diameter: 0.12–0.3 mm; length: 1.5–3.1 mm) and lamellar crystals (thickness of 200–300 nm and average area of 0.21 mm 2 ) were obtained by effectively manipulating the diameter and effective length of jet flow.
研究领域:
化学工程
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
Materials
·
2025
·Vol. 18
·期 5
·1079
·
IF 3.2
Baiyi Li,Guangdong Zhou
In this study, various phosphate glasses (PGs) within the CaO-Na2O-P2O5and SiO2-CaO-Na2O-P2O5systems were synthesized using both conventional and microwave melt-quenching techniques. The physical, thermal, and structural properties of these phosphate glasses, as well as their dissolution behaviors under varying temperatures and solvent conditions, were thoroughly examined. Additionally, the influence of scale inhibitor dosage, temperature, and pH on the rate of scale inhibition was assessed using a static scale inhibition test. The morphology and crystal types of the precipitates were characterized using Scanning Electron Microscopy (SEM) and X-ray Diffraction (XRD) analysis. The findings indicate that the PG structure predominantly consists of Q2structural units, with a minor presence of Q1units. The dissolution rate of PG escalates with an increase in temperature and a decrease in pH. Conversely, the scale inhibition efficiency diminishes with rising temperature and pH. Optimal scale inhibition efficiency, reaching up to 95.4%, was observed at a pH of 7 and a temperature of 50 °C. Under the influence of the PG scale inhibitor, the primary crystal form of CaCO3was altered from calcite to vaterite.
研究领域:
材料科学
·
化学工程
✓ 已验证
DOI
PubMed
高影响因子
Oxidative Medicine and Cellular Longevity
·
2021
·
IF 6.543
Wei-Bo Huang,Guo-Jun Zou,Gu-Hua Tang,Xin-Yuan Sun,Jian-Ming Ouyang
The original Laminaria polysaccharide (LP0) was sulfated using the sulfur trioxide-pyridine method, and four sulfated Laminaria polysaccharides (SLPs) were obtained, namely, SLP1, SLP2, SLP3, and SLP4. The sulfated (–OSO3–) contents were 8.58%, 15.1%, 22.8%, and 31.3%, respectively. The structures of the polysaccharides were characterized using a Fourier transform infrared (FT-IR) spectrometer and nuclear magnetic resonance (NMR) techniques. SLPs showed better antioxidant activity than LP0, increased the concentration of soluble Ca2+ in the solution, reduced the amount of CaOx precipitation and degree of CaOx crystal aggregation, induced COD crystal formation, and protected HK-2 cells from damage caused by nanometer calcium oxalate crystals. These effects can inhibit the formation of CaOx kidney stones. The biological activity of the polysaccharides increased with the content of –OSO3−, that is, the biological activities of the polysaccharides had the following order: LP0 < SLP1 < SLP2 < SLP3 < SLP4. These results reveal that SLPs with high –OSO3− contents are potential drugs for effectively inhibiting the formation of CaOx stones.
研究领域:
药理学
·
材料科学
·
细胞生物学
✓ 已验证
DOI
PubMed
高影响因子
DESALINATION
·
2025
·
IF 9.8
Dong Guo,Haixu Hou,Yuansheng Pei,Li'an Hou
Mineral deposits in circulating water circuits impair thermal efficiency and restrict fluid flow, thereby threatening production safety. The excellent performance of traditional scale inhibitors, such as phosphonates, is accompanied by nitrogen and phosphorus loads in concentrated water, emphasizing the necessity for sustainable alternatives. This study proposes that polyborates have the potential to serve as a new generation of sustainable scale inhibitors, however, their structural optimization lacks theoretical guidance. Therefore, this research examines the electronic distribution and bonding characteristics of different boron hybridization types, specifically [BO 2 ], [BO 3 ], and [BO 4 ] units, and their effects on the scaling inhibition process. The results reveal that sp-hybridized [BO 2 ] units are resistant to hydrolysis, and their chemical stability leads to unsatisfactory scale inhibition performance. In contrast, sp 2 -hybridized [BO 3 ] units exhibit affinity for calcite, inducing the transformation of calcite into vaterite, thereby suppressing scale deposition. The hydrolysis of oxygen-bridged sp 2 -hybridized boron to form sp 3 -hybridized [BO 4 ] units represents a precursor step in the complete hydrolysis and dehydrogenation of polyborates. The resulting borate ions complex with calcium in the form of CaBO 3 , effectively inhibiting calcite crystal nucleation. When a solution contains 0.03 mmol/L of B 5 O 6 (OH) 4 − , which includes four [BO 3 ] units, it can reduce the growth rate of calcite crystals by up to 65.4 %. Understanding the relationship between boron hybridization types and inhibition mechanisms provides critical insights for designing optimal polyborates, representing a significant advancement in the development of sustainable water treatment solutions.
研究领域:
化学工程
·
环境科学
✓ 已验证
DOI
高影响因子
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
·
2025
·
IF 4.9
Zhaoyu Wang,Qingxia Ji,Tingting Wang,Weiliang Hua,Yanhong Bi,Yongzhou Chi,Rongling Yang
Schisantherin A (SCA) has recently attracted increasing attention because of its promising biological profile. However, poor aqueous solubility and bioavailability have limited the efficacy of SCA in pharmaceutical applications. In this study, novel complex CaCO 3 -based biomineralized microparticles that demonstrate significant potential as carriers for loading and delivering SCA were constructed for the first time by employing gum arabic as the stable template and polydopamine as the functional coating agent. The results show that these composite microparticles have a high SCA loading rate of 15.6 ± 0.4 % and a pH-responsive drug release characteristic under simulated in vitro conditions. Further investigation revealed that the encapsulated forms of SCA exhibit significantly enhanced synergistic antioxidant activity and antibacterial efficacy against Staphylococcus aureus compared to the carrier-free SCA. Furthermore, a 4′,6-diamidino-2-phenylindole (DAPI) staining experiment and a rhodamine 123 (Rh123) fluorescence staining test of lipopolysaccharide (PSL) induced BV-2 microglial cells demonstrated that the encapsulated form of SCA exhibits considerable efficacy in inducing changes in the morphology and structure of cells. With these findings, this study provides a new type of CaCO 3 -mediated particle carrier that is useful for targeted and controlled release applications.
研究领域:
药理学
·
材料科学
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
Biomaterials Science
·
2023
·Vol. 11
·期 10
·3524-3546
·
IF 6.6
Jing-Hong Liu,Jian-Ming Ouyang
Objective: The first objective is to study the synergistic inhibition of calcium oxalate (CaOx) formation by Laminarin polysaccharides (DLP and SDLP, before and after sulfation) and potassium citrate (K3cit) and determine the synergistic protection of renal epithelial cells (HK-2 cells) caused by CaOx crystal damage. The second objective is to explore new ways to prevent and treat kidney stones. Methods: The CaOx crystals regulated by five additives (K3cit group, DLP group, SDLP group, DLP–K3cit synergistic group and SDLP–K3cit synergistic group) were characterized by FT-IR, XRD, SEM, zeta potential, ICP, and TGA. The protective effect of each additive group on HK-2 cells damaged by nano-calcium oxalate monohydrate (nano-COM) was compared by detecting cell viability, the cell reactive oxygen species level, the cell survival rate, and mitochondrial membrane potential. Results: When DLP or SDLP acted synergically with K3cit, the synergistic group induced the same amount of COD at a lower concentration or more COD formation at the same concentration, highlighting the synergistic enhancement effect of 1 + 1 > 2. At 0.3 g L−1, the COD contents induced by DLP, SDLP, K3cit, DLP–K3cit, and SDLP–K3cit synergistic groups were 20.3%, 75.8%, 75.4%, 87.3%, and 100%, respectively. The synergistic group increased the concentration of soluble Ca2+ ions in the supernatant, increased the absolute value of the zeta potential on the surface of CaOx crystals, and inhibited the aggregation among the crystals. TGA and DTG analyses established the adsorption of polysaccharides in the crystals. Cell experiments showed the ability of the synergistic group to significantly inhibit the damage of nano-COM crystals on HK-2 cells, reduce the level of reactive oxygen species and mortality, and improve cell viability and the mitochondrial membrane potential. Conclusions: The synergistic group can more effectively induce COD formation and cell protection than the standalone polysaccharide group or K3cit group. The synergistic groups, especially SDLP–K3cit, may be a potential drug for inhibiting the formation of CaOx kidney stones.
研究领域:
医学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF NANOBIOTECHNOLOGY
·
2025
·Vol. 23
·期 1
·1-24
·
IF 12.6
Tong Xin-Yi,Nong Wei-Jian,Tan Run-Min,Ouyang Jian-Ming
Objective This study aimed to compare the differences in pyroptosis and polarization of macrophages induced by different sizes of calcium oxalate monohydrate (COM) crystals and discuss the mechanism of kidney stone formation. Methods COM crystals with sizes of about 100 nm, 500 nm, 2.5 μm, and 10 μm were synthesized and characterized. THP-1 cells were induced to differentiate into macrophage-like cells. The damage of COM crystals to Mφs was evaluated by CCK-8 assay. The activation of the pyroptosis signaling pathway, cell morphology, and proportion of pyroptosis cells in Mφs induced by COM crystals were detected. Finally, the proportion of M1/M2 polarization was qualitatively and quantitatively assessed. Results COM-100 nm, COM-500 nm, COM-2.5 μm, and COM-10 μm all significantly induced the pyroptosis and polarization of Mφs. Among them, at the same cell viability, COM-10 μm induced the highest pyroptosis rate. The expression of NLRP3 and GSDMD-N induced by COM-10 μm increased by 81.43 ± 11.45% and 112.00 ± 9.87%, respectively, and the rate of caspase-1/PI double-positive pyroptosis cells was 22.2%. Meanwhile, COM-10 μm induced the highest proportion of M1 polarization (M1/M2 ratio was 209.90% ± 2.54%). Conclusion COM-10 μm significantly activates the classical caspase-1-mediated pyroptosis pathway and promotes M1 polarization in Mφs. Therefore, at the same cell viability, large-sized micro-COM is more capable of inducing inflammation than nano-COM. Graphical
研究领域:
纳米技术
·
泌尿科学
·
免疫学
·
细胞生物学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Small
·
2024
·
IF 13
Yu He,Ejun Peng,Xiaozhuo Ba,Jian Wu,Wen Deng,Qiu Huang,Yonghua Tong,Haojie Shang,Zichen Zhong,Xiao Liu,Yanlong Zhang,Tao Ye,Xiaoqi Yang,Kangyang Wang,Yabin Xie,Kehua Jiang,Ding Xia,Zhiqiang Chen,Kun Tang
Emerging studies have demonstrated that M1 macrophage polarization and oxidative stress play important roles in calcium oxalate (CaOx) induced kidney injury, which leads to increased crystals deposition. ROS scavenging nanozymes and kidney-targeted nanoparticles for antioxidant drugs delivery have emerged as an arisen methodology for kidney injury therapy. However, cell membrane biomimetic-modified nanozymes as anti-inflammatory drug delivery systems for the treatment of kidney injury is rarely reported. Herein, the ROS responsive red blood cell-membrane-coated resatorvid-loaded cerium oxide nanoparticles (RBCM@CeO 2 /TAK-242) are constructed to suppress CaOx induced kidney injury and crystals deposition. In vitro, RBCM@CeO 2 /TAK-242 shows effective internalization by renal tubular epithelial cells, along with demonstrated antioxidative, anti-inflammatory, and macrophage reprogramming effects. Glyoxalate(Gly)-induced renal CaOx crystals mouse model is established, RBCM@CeO 2 /TAK-242 shows excellent injured kidney targeting and biosafety, and could effectively suppress CaOx induced kidney injury and crystals deposition. RBCM@CeO 2 /TAK-242 has a dual protective effect by both inhibiting oxidative stress and modulating macrophage polarization in vivo. In addition, RNA seq analysis reveals that RBCM@CeO 2 /TAK-242 protects against CaOx induced kidney injury via suppressing the TLR4/NF-κB pathway. This study provides an innovative strategy for RBCM@CeO 2 /TAK-242 as injured kidney targeting and dual protective effects for the treatment of CaOx induced kidney injury and crystals deposition.
研究领域:
纳米技术
·
药理学
·
肾脏生理学
·
炎症
·
氧化应激
·
药物递送系统
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2025
·
IF 9
Han Li,Yushan Li,Huailing Diao,Haipeng Ren,Jianming Chen,Jinfeng Wang,Qingtao Liu,Xungai Wang
Wild silk cocoons are light-weight composite structures with unique properties. One such property is the intriguing CO 2 gating property (i.e., unidirectional flow of CO 2 ) exhibited by the wild Antheraea pernyi ( A. pernyi ) silkworm cocoon. This study investigated the hierarchical structural and compositional features of A. pernyi cocoons and their impact on CO 2 adsorption. For the first time, soap-film flowmeter testing method and gas permeability testing method were used to quantitatively demonstrate the A. pernyi cocoons’ CO 2 adsorption property and selectivity for gas mixture. The results showed that the multi-layered composite structure of A. pernyi cocoons, coupled with calcium oxalate crystals, play a crucial role in their CO 2 adsorption and separation performance. These findings shed light on the unique functional design of wild silk cocoons and provide a new biomimetic approach for developing materials and structures to address global challenges such as CO 2 capture and storage.
研究领域:
材料科学
·
环境科学
·
仿生学
·
气体分离
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）