Chemical and Biological Technologies in Agriculture
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2023
·Vol. 10
·期 1
·1-12
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IF 6.6
Zheng Lei,Zhu XingYi,Wang Jialu,Su Weike
Mechanochemical-assisted extraction (MCAE) and mechanochemical-assisted enzyme catalysis (MCAEC) methods were developed for extraction and enzymatic hydrolysis of calcium phytate from defatted rice bran, respectively.The MCAE conditions were optimized by response surface methodology: 10% sodium carbonate as the solid reagent, 33 min grinding time, 18:1 ball–material ratio, 330 rpm ball milling speed, and pH 5.5 extraction. Under these conditions, the yield of calcium phytate was 97.0 mg/g. The MCAEC conditions were optimized as follows: 2% enzyme addition, MM400 milling time 36 min, PM400 milling speed 262 rpm, MM400 milling frequency 27 Hz, and the addition of pH 5.5 sodium acetate buffer 3 mL. Under these conditions, the hydrolysis rate of calcium phytate was 39.7%.Compared with traditional extraction and enzymatic hydrolysis methods, MCAE and MCAEC could obtain higher yields and hydrolysis rates, respectively.
研究领域:
食品科学
·
化工工程
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
ACS Biomaterials Science & Engineering
·
2023
·
IF 5.8
Shuo Dong,Tong Zhao,Wei Wu,Zhewei Zhang,Jin Wu,Kunzhan Cai,Guoqing Li,Jiaxin Lv,Heyang Zhou,Chunbo Tang
Hyperglycemia in patients with diabetes affect osteoblast function, leading to abnormal bone metabolism and implant failure. Adequate bone volume surrounding an implant is essential for osseointegration, which can be improved by implant surface modifications. In this study, titanium surfaces were hydrothermally treated with a mixture of phytic acid (PA) and calcium hydroxide to produce a calcium-decorated surface. The control group comprised pure titanium with a sandblasted/acid-etched (SLA) surface. The elemental composition, hydrophilicity, surface roughness, and morphology of the titanium surfaces were examined. Evaluation of in vitro osteogenic differentiation ability in a high-glucose environment using alkaline phosphatase (ALP) staining, ALP activity assays, Alizarin Red S staining, quantitative reverse transcription-polymerase chain reaction (qRT-PCR), and immunofluorescence staining revealed that Ca-PA-modified SLA titanium surfaces can promote osteogenic differentiation of human bone marrow mesenchymal stem cells (hBMSCs). Evaluation of oxidative stress and aging using reactive oxygen species (ROS), malondialdehyde (MDA), superoxide dismutase (SOD), and β-galactosidase staining revealed that Ca-PA-modified SLA titanium surfaces can reduce ROS production and ameliorate oxidative stress damage in hBMSCs. In vivo assessment of osteogenesis in a diabetic rat model revealed that Ca-PA coating promotes peri-implant osseointegration. Ca-PA-modified SLA titanium surface is a candidate for improving implant osseointegration in patients with diabetes.
研究领域:
生物材料学
·
再生医学
·
口腔医学
✓ 已验证
DOI
PubMed
高影响因子
WATER RESEARCH
·
2025
·
IF 12.4
Lan Lei,Rui Zhang,Rui-Xiang Bi,Zhi-Hai Peng,Xin Liu,Tie-Ying Shi,Li Zhang,Ru-Ping Liang,Jian-Ding Qiu
Efficient uranium capture from rare earth tailings wastewater holds great importance for human health and sustainable development. Herein, we present a simple and eco-friendly approach to form a single network hydrogel through electrostatic interaction between chitosan and sodium alginate. Subsequently, calcium phytate is introduced as a natural crosslinking agent to generate a secondary cross-linked network, leading to a composite hydrogel (CS-SA/PCa) with a doubly enhanced network structure for efficient adsorption of uranium from wastewater. The established multistage porous structure enables the rapid diffusion of uranyl ions, and the abundant phosphate groups serving as adsorption sites can offer high affinity for U(VI). Most importantly, CS-SA/PCa is formed through physical cross-linking of sustainable biopolymers, avoiding the use of toxic chemical agents. In addition, CS-SA/PCa exhibited significantly better mechanical properties than those of single-network physical hydrogels crosslinked by electrostatic interactions, which overcame the weak mechanical properties of physical hydrogels. It provides a new method for the manufacture of environmentally friendly, low-cost and robust physical hydrogels based on natural polymers.
研究领域:
环境科学
·
材料科学
·
生物工程
✓ 已验证
DOI
PubMed
高影响因子
ORAL DISEASES
·
2023
·
IF 3.8
Jiaxin Lv,Qiaona Wang,Dongyu Liu,Catherine Huihan Chu,Heyang Zhou,Guoqing Li,Jin Wu,Kunzhan Cai,Chunbo Tang
Objectives Diabetes mellitus (DM) induces oxidative tissue impairment and suppresses bone formation. Some studies have shown that phytic acid has antioxidant and anti-diabetic properties. This study aimed to investigate the potential of calcium phytate (Ca-phytate) to reverse inhibited osteogenesis of human bone marrow mesenchymal stem cells (hBMSCs) in a high glucose (HG) environment and to determine the underlying mechanism. Materials and Methods hBMSCs were exposed to HG and palmitic acid to simulate DM in vitro. Osteogenic differentiation was measured using alkaline phosphatase staining and activity assay, alizarin red S staining, qRT-PCR, Western blot and immunofluorescence staining. A critical-size cranial defect model of type 2 diabetes mellitus (T2DM) rats was established to evaluate bone regeneration. A specific pathway inhibitor was used to explore whether the MAPK/JNK pathway was involved. Results Treatment with 34 μM Ca-phytate had the highest effect on osteogenic differentiation in HG. Ca-phytate improved cranial bone defect healing in T2DM rats. The long-term HG environment inhibited the activation of the MAPK/JNK signalling pathway, which was restored by Ca-phytate. Blocking the JNK pathway reduced the Ca-phytate-mediated osteogenic differentiation of hBMSCs. Conclusion Ca-phytate induced bone regeneration in vivo and reversed HG-inhibited osteogenesis of hBMSCs in vitro via the MAPK/JNK signalling pathway.
研究领域:
医学
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细胞生物学
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内分泌学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）