JOURNAL OF MEMBRANE SCIENCE
·
2019
·
IF 7.015
Bingbing Yuan,Pengfei Li,Honghong Sun,Shengchao Zhao,Peng Li,Haixiang Sun,Q. Jason Niu
Polyamide membranes based on trimesoyl chloride (TMC) have captured the majority of the market share for desalination in water treatment. The TMC based membrane is prone to form calcium bridges with the organic foulants due to the relatively strong deprotonation ability of the benzoic acid generated from the incomplete reaction of the acyl chloride monomers . The foulants are thus rapidly deposited on the surface of the TMC based membrane, which results in the deterioration of water production and decrease of membrane lifespan. Herein, we have fabricated a polyamide membrane based on novel trimesoyl-[4,4-dimethyl-5(4H)-azlactone] (TMDMA) monomer instead of TMC monomer. Since the carboxylic groups of the prepared TMDMA polyamide nanofilm are not directly connected to the phenyl ring, the acidity coefficient ( p K a ) value is higher and, consequently, deprotonation ability is expected to be weaker. To confirm this theory, we have conducted the fouling experiment on TMDMA based membrane using alginate as a model foulant and compared that with TMC based polyamide membranes. A lower flux decline (33%) and higher cleaning efficiency (90.72%) are observed for the TMDMA polyamide membrane, while the flux decline and cleaning efficiency for the TMC based polyamide membrane are 52% and 49.52% respectively. Furthermore, the average Ca 2+ density of the TMDMA polyamide membrane surface before and after cleaning are 81.52 mg m −2 and 21.61 mg m −2 respectively, which are lower than that of the TMC based polyamide membrane surface. Thus, it is clearly showing that the surface of the TMDMA polyamide membrane is not prone to form calcium bridges with foulants due to the weaker complexation between Ca 2+ and the carboxylic acid groups.
研究领域:
材料科学
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环境工程
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产品关联
DOI
高影响因子
INTERNATIONAL UROLOGY AND NEPHROLOGY
·
2020
·Vol. 52
·期 7
·1227-1234
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IF 1.843
Wang Zhenghao,Bai Yunjin,Wang Jiahao,Wang Jia
Objective To investigate the effect of α-lipoic acid (α-LA) in the prevention and treatment of ethylene glycol-induced calcium oxalate deposition in a rat model and preliminary exploration of the mechanism. Methods Sixty male Wistar rats were divided randomly and equally into six groups including two α-LA prevention groups, two α-LA therapeutic groups, one controlled group and one intervention group. Besides controlled group, other group received 1% glycol solution and 2 ml 2% ammonium chloride for 4 weeks as a stone inducer. The prevention groups received 0.1 and 0.2 mg/kg body weight/day/rat α-LA as food supplement during inducing stone and therapeutic groups received 0.1 and 0.2 mg/kg body weight/day/rat α-LA for 4 weeks after 4 weeks stone inducing. Results The volume of urine, the pH and magnesium levels in the preventive and therapeutic groups were higher in a dose-independent manner ( p < 0.05), and urinary calcium was lower ( p < 0.05). Antioxidant stress enzyme activity (glutathione, superoxide dismutase, catalase) in serum and kidney homogenates in the preventive and therapeutic groups underwent significant regeneration ( p < 0.05) and malondialdehyde levels and the production of free radical moieties decreased. Pathological observation demonstrated that there was deformation due to renal tubular expansion in the control group, greater visible inflammatory cell infiltration into the interstitial spaces and partial destruction of the glomerular structures. α-lipoic acid improved the lesions to varying degrees. The extent of crystal deposition was lower in the preventive and therapeutic groups compared with the control group ( p < 0.05). Conclusion The present study indicated that α-LA provides both preventive and therapeutic effects against the deposition of calcium oxalate crystals in rats.
研究领域:
医学
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药理学
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泌尿学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
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2023
·Vol. 170
·期 12
·122505
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IF 3.9
Yongxin Wu,Muya Cai,Hongya Wang,Zuojun Hu,Fangzhao Pang,Xiang Chen,Meiyu Zhao,Bingbing Wang,Xiong Zhang,Xiaowei Liu,Dihua Wang,Huayi Yin
Lead-acid batteries (LABs) are commonly utilized in various applications such as electric motorcycles, uninterruptible power systems, and stationary energy storage devices. Calcium (Ca) is an essential element being added to Pb grid alloys to increase their mechanical strength. In this paper, molten salt electrolysis is used to prepare Pb-Ca master alloy in molten CaCl2-NaCl at 700 °C. The Ca concentration reaches 2.51 wt%, and the current efficiency reaches 85.0% for the 1 A-scale electrolyzer and 75.8% for the 50 A-scale cell. Compared with the traditional physical mixing ways, Ca is selectively and gradually deposited in the liquid Pb cathode without worrying about the immense heat generated by mixing Ca and Pb. In addition, molten salt serves as a media to avoid the oxidation of Ca. Through thermodynamic calculations and electrochemical measurements, the Pb-Ca formation prefers to happen rather than Pb-Na alloy in molten CaCl2-NaCl because of the stronger interaction of Ca and Pb than Na and Pb. Overall, this paper provides a straightforward strategy to prepare Pb-Ca master alloys, which can be expanded to prepare various liquid semimetal alloys containing reactive metals.
研究领域:
材料科学
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冶金工程
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电化学
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能源材料
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功能合金
✓ 已验证
DOI
高影响因子
Journal of Magnesium and Alloys
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2021
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IF 10.088
Youwen Yang,Changfu Lu,Lida Shen,Zhenyu Zhao,Shuping Peng,Cijun Shuai
Biodegradable magnesium (Mg) and its alloy show huge potential as temporary bone substitute due to the favorable biocompatibility and mechanical compatibility. However, one issue deserves attention is the too fast degradation. In this work, mesoporous bioglass (MBG) with high pore volume (0.59 cc/g) and huge specific surface area (110.78 m 2 /g) was synthesized using improved sol-gel method, and introduced into Mg-based composite via laser additive manufacturing. Immersion tests showed that the incorporated MBG served as powerful adsorption sites, which promoted the in-situ deposition of apatite by successively adsorbing Ca 2+ and HPO 4 2− . Such dense apatite film acted as an efficient protection layer and enhanced the corrosion resistance of Mg matrix, which was proved by the electrochemical impedance spectroscopy measurements. Thereby, Mg based composite showed a significantly decreased degradation rate of 0.31 mm/year. Furthermore, MBG also improved the mechanical properties as well as cell behavior. This work highlighted the advantages of MBG in the fabrication of Mg-based implant with enhanced overall performance for orthopedic application.
研究领域:
生物医学工程
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材料科学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF POLYMER RESEARCH
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2013
·Vol. 20
·期 6
·1-10
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IF 2.019
Yang Jiaojiao,Cao Shuqin,Xin Jianyu,Chen Xingyu,Wu Wei,Li Jianshu
Layer-by-layer (LbL) systems constructed via electrostatic attraction or other forces can be used as templates in biomimetic mineralization. In this work, 21-arm star poly(acrylic acid) (star-PAA) and 21-arm star poly[2-(dimethylamino)ethyl methacrylate] (star-PDMAEMA) were successfully synthesized from a cyclodextrin core via atom transfer radical polymerization (ATRP). The star polymers were used to construct three kinds of LbL systems: negatively charged star-PAA with positively charged chitosan (CHI) as a model of unconfined space for mineralization, and positively charged star-PDMAEMA with negatively charged poly(styrene sulfonic acid) sodium salt (PSS), which had acid-etched holes or constructed within a porous polycarbonate filters, as two different models of confined space for mineralization. Different crystal forms of calcium carbonate were obtained using the three systems, so these LbL systems assembled from star polymers could be new tools for developing functional materials and investigating fundamental aspects of the mineralization process.
研究领域:
材料科学
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仿生学
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化学工程
✓ 已验证
DOI
高影响因子
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY
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2016
·Vol. 39
·期 4
·1391-1403
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IF 4.652
Zhang Lei,Jiang Guoliang,Zhao Xueling,Gong Yuekun
Background/Aims: We investigated the role of dimethyloxalylglycine (DMOG) in bone marrow mesenchymal stem cell (BMSC) osteogenesis mediated by RhoA/ROCK. Methods: BMSCs were cultured with and without DMOG and/or Y-27632 (ROCK1 inhibitor). Cell proliferation, alkaline phosphatase (ALP) levels, and calcium deposits were determined. The expression of Runx2, OSX, p-cofilin, RhoA, and GTP-bound RhoA was determined by real-time RT-PCR and Western blot. Rho-associated coiled-coil-containing protein kinase (ROCK) activity was determined by measuring the phosphorylation of myosin-binding subunit of myosin phosphatase using an ELISA kit. Actin morphology was observed by immunofluorescence. Results: After 24 h, DMOG (0.5 mM) increased the expression of GTP-bound RhoA (+141%, P < 0.001) and enhanced ROCK activity (315%, P < 0.001). DMOG (0.5 mM) enhanced ALP levels after 3, 7, and 21 days of osteogenic induction (all P < 0.001) and strengthened calcium deposition (P < 0.001). In addition, compared with controls, DMOG (0.5 mM) increased the mRNA levels of osteogenesis genes RUNX2 and OSX (all P < 0.001). Furthermore, compared with controls, DMOG increased the expression of p-cofilin (+57%, P < 0.001), which resulted in rearrangement of actin filaments. All these effects were abolished, at least in part, by Y-27632. Conclusion: DMOG promotes BMSC osteogenic differentiation via activation of RhoA/ROCK, suggesting clues for future therapies using BMSCs
研究领域:
干细胞生物学
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骨骼生物学
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细胞信号传导
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分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Biomaterials Science & Engineering
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2020
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IF 4.152
Lei Liu,Yali Miao,Xuetao Shi,Huichang Gao,Yingjun Wang
Phosphorous-containing biopolymers have been applied to expedite the regeneration of damaged bone tissue by stimulating the function of phosphorous groups in natural bones. However, the underlying mechanism of phosphorous-containing biopolymers in promoting osteogenic differentiation is unclarified. Herein, we synthesized phosphorylated chitosan hydrogels by incorporating phosphocreatine into chitosan molecular chains under mild conditions. The introduction of phosphate groups improved properties of protein adsorption and calcium deposition without affecting the morphology of hydrogels. Our results showed that phosphorylated chitosan hydrogels could not only promote alkaline phosphatase activity and mineralization but also upregulate the expression of osteogenic-related genes and proteins. Meanwhile, application of c-Jun N-terminal kinase inhibitor SP600125 and p38 mitogen-activated protein kinase inhibitor SB203580 repressed the expression of osteogenic-related markers in gene and protein levels. To the best of our knowledge, it is reported for the first time that phosphorous-containing biopolymers promote osteogenic differentiation of osteoblasts via JNK and p38 signaling pathways.
研究领域:
生物材料
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组织工程
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骨再生
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细胞信号
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2015
·
IF 6.723
Mengke Wang,Yi Deng,Ping Zhou,Zuyuan Luo,Qiuhong Li,Bingwu Xie,Xiaohong Zhang,Tong Chen,Duanqing Pei,Zhihui Tang,Shicheng Wei
Human pluripotent stem cells (hPSCs) are a promising cell source with pluripotency and capacity to differentiate into all human somatic cell types. Designing simple and safe biomaterials with an innate ability to induce osteoblastic lineage from hPSCs is desirable to realize their clinical adoption in bone regenerative medicine. To address the issue, here we developed a fully defined synthetic peptides-decorated two-dimensional (2D) microenvironment via polydopamine (pDA) chemistry and subsequent carboxymethyl chitosan (CMC) grafting to enhance the culture and osteogenic potential of hPSCs in vitro. The hPSCs including human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) were successfully cultured on the peptides-decorated surface without Matrigel and ECM protein coating and underwent promoted osteogenic differentiation in vitro, determined from the alkaline phosphate (ALP) activity, gene expression, and protein production as well as calcium deposit amount. It was found that directed osteogenic differentiation of hPSCs was achieved through a peptides-decorated niche. This chemically defined and safe 2D microenvironment, which facilitates proliferation and osteo-differentiation of hPSCs, not only helps to accelerate the translational perspectives of hPSCs but also provides tissue-specific functions such as directing stem cell differentiation commitment, having great potential in bone tissue engineering and opening new avenues for bone regenerative medicine.
研究领域:
干细胞生物学
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组织工程
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再生医学
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生物材料科学
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细胞工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2018
·Vol. 8
·期 63
·36331-36337
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IF 2.936
Huishang Yang,Shenggui Chen,Lei Liu,Chen Lai,Xuetao Shi
Phosphorylated biopolymers can induce mineralization, mimic the process of natural bone formation, and have the potential as scaffolds for bone tissue engineering. 2-Methacryloyloxyethyl phosphorylcholine (MPC), a low cytotoxicity phosphorus source, is mainly applied in vascularization and promoting blood compatibility and has been less researched for bone repair. In this study, phosphorylated methacrylamide chitosan (PMAC) hydrogel was prepared by mixing methacrylamide chitosan (MAC) and different mass of MPC with photoinitiator under UV irradiation. A series characterization tests showed that PMAC hydrogels were successful prepared and had a pretty good mineralization ability. Moreover, human fetal osteoblastic (hFOB) cells cultured on PMAC hydrogels exhibited not only highly viability but also the enhanced ALP activity and calcium deposition. The PMAC hydrogels have great potential in bone tissue engineering applications.
研究领域:
材料科学
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生物医学工程
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组织工程
✓ 已验证
DOI
PubMed
高影响因子
Frontiers in Bioengineering and Biotechnology
·
2023
·
IF 5.7
Liu Qiaoyun,Liu Changjiang,Wang Weixing,Yuan Liangjie,Wang Yu,Yi Xinzeyu,Pan Zhenyu,Yu Aixi
Bioinspired strontium magnesium phosphate cements for bone tissue engineering were prepared using a new, facile, environmentally friendly and high yielding (98.5%) precursor method. The bioinspired SMPCs have uniform particle distributions, excellent mechanical strengths and high biocompatibilities. The in vitro responses of bone marrow stromal cells to the SMPCs, including viability, osteogenic differentiation and alkaline phosphatase activity, were evaluated. The results show that the SMPC containing 0.5 mol of strontium (referred to as SMPC-2) has a higher degradation rate and biological activity than magnesium phosphate cements and the other SMPCs. In addition, the synergistic effect of strontium and magnesium ion release from SMPC-2 creates a conducive environment for cell proliferation, mineralized calcium deposition and new bone formation. These observations demonstrate the feasibility of using the new precursor method to generate SMPCs and the utility of these biologically compatible and highly effective cements for bone tissue engineering.
研究领域:
材料科学
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生物医学工程
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生物材料
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组织工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nanoscale
·
2020
·Vol. 12
·期 35
·18225-18239
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IF 6.895
Kun Liu,Ling Zhu,Shengyue Tang,Wei Wen,Lu Lu,Mingxian Liu,Changren Zhou,Binghong Luo
Although poly(L-lactide) (PLLA) based porous scaffolds have been widely fabricated through 3D printing, their poor mechanical properties and osteogenic activity still do not meet the needs of bone tissue repair. Herein, chitin whiskers (CHWs), having outstanding mechanical properties, excellent cell affinity, osteogenic activity, etc. were designed to introduce into the PLLA matrix. Moreover, a trisolvent system, including dichloromethane (DCM), 2-butoxyethanlol (2-Bu) and dibutyl phthalate (DBP), instead of a single solvent system of DCM was chosen to prepare CHW/PLLA (CP) composite inks. Then, the CP porous composite scaffolds were further fabricated via the direct ink writing method. The as-printed CP composite scaffolds have good 3D porous structures with a pore size of 400 ± 14 μm and a porosity of 80 ± 5%. Compared with the pure PLLA scaffold, the CP composite scaffolds showed significantly superior hydrophilicity and compression performance, and also were more conducive to cell adhesion, proliferation, and up-regulating alkaline phosphate activity and calcium deposition due to the presence of CHWs. Moreover, these promoting effects of CHWs are positively related to the content of the whiskers in the range of 0–20 wt%. However, as the content of CHWs further increased to 40 wt%, the compression performance, cell affinity and osteogenic activity of the corresponding 40%CP composite scaffold decreased, which may be attributed to the different microstructure of the scaffold from other composite scaffolds. Interestingly, compared with these scaffolds containing a lower mass content of CHWs, only the 40%CP composite scaffold exhibited significant anti-inflammatory properties. These robust CP composite scaffolds offer a new route for bone tissue engineering application.
研究领域:
材料科学
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生物医学工程
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组织工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Advanced Healthcare Materials
·
2021
·Vol. 10
·期 12
·2100196
·
IF 9.933
Chunxia Ren,Xinqing Hao,Lu Wang,Yue Hu,Lin Meng,Shize Zheng,Feilong Ren,Wenhuan Bu,Huan Wang,Daowei Li,Kai Zhang,Hongchen Sun
The osteogenic potential of mesenchymal stem cells (MSCs) is severely impaired under persistent inflammation of periodontitis. A highly efficient way to promote or rescue osteogenic potential of MSCs under inflammation remains an unmet goal. Herein, metformin carbon dots (MCDs) with excellent biocompatibility are prepared from metformin hydrochloride and citric acid via a hydrothermal method. The MCDs can more effectively enhance the alkaline phosphatase (ALP) activity, calcium deposition nodules formation, expression of osteogenic genes and proteins in rat bone marrow mesenchymal stem cells (rBMSCs) than metformin under both inflammatory and normal conditions. Moreover, a novel pathway of extracellular signal-regulated kinases (ERK)/AMP-activated protein kinase (AMPK) signaling is involved in the MCDs-induced osteogenesis. In periodontitis rats, MCDs can effectively regenerate the lost alveolar bone, but not the metformin. Taken together, MCDs can be the promising candidate nanomaterial for periodontitis treatment. This work may provide a new pharmacological target of ERK/AMPK pathway for treating bone loss and also give additional insights into developing nanodrugs from the numerous medications.
研究领域:
药理学
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牙科科学
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纳米技术
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干细胞生物学
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分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）