Talanta Open
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2025
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IF 3.7
Wenju Ren,Chenyang Qi,Rui Yang,Jie Zhou,Liu Yang,Yuandi Yu,Taixiong Zheng,Lizhi Fu
African Swine Fever (ASF) poses a severe threat to the global pig husbandry. In the absence of an effective vaccine, developing rapid and accurate diagnostic methods is crucial for epidemic control. This study designed a low-cost microfluidic chip and integrated nucleic acid rapid detection device (INARDD) based on colorimetric principles combined with magnetic bead based nucleic acid extraction technology. Manufactured using micro computer numerical control machine tools (CNC) and 3D printing, the device fully automates the entire process include nucleic acid extraction, transfer, loop-mediated isothermal amplification (LAMP), and detection without requiring external equipment or manual intervention. Experimental results demonstrate high sensitivity (94.1 %, 32/34), specificity (97.7 %, 42/43), and accuracy (96.1 %, 74/77), with a detection limit of 10² copies/μL. The device features low production costs (approximately $69.1), low per-test expenses, and a portable design (2 kg), facilitating field deployment. This study provides an economical and efficient solution for rapid on-site diagnosis of African swine fever, holding significant implications for enhancing epidemic control and ensuring meat safety. It also offers technical reference for developing portable diagnostic systems for other infectious diseases.
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TOXICOLOGY LETTERS
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2026
·
IF 3.1
Tianyi Chen,Fengjun Fan,An Cheng,Yumei Xia,Haoze Chen,Jiakai Fang,Yangyu Chen,Tianjia Li,Anli Wang,Binjie Wang,Weixuan Yao,Yuanzhao Wu
Flocoumafen (FCF), a second-generation anticoagulant rodenticide (SGAR), has limited toxicological data regarding its effects on aquatic organisms. In this study, we exposed zebrafish embryos to FCF solutions at concentrations of 0.2, 0.4, and 0.8 mg/L until 120 h post-fertilization (hpf). The results revealed a decrease in survival rates. Notably, FCF exposure significantly reduced the frequency of spontaneous tail coils at 24 hpf, while shortened body length and induced spinal curvature at 120 hpf. Furthermore, zebrafish larvae exhibited craniofacial abnormalities and incomplete bone mineralization at 120 hpf following FCF exposure. In Tg( HuC:EGFP ) transgenic strains, neuronal loss was observed. Additionally, FCF-exposed zebrafish larvae showed a marked reduction in locomotor ability, activity levels, and turning capacity. The qPCR and enzyme activity assays revealed significant changes in gene expression associated with the Notch signaling pathway, accompanied by increased levels of reactive oxygen species (ROS), superoxide dismutase (SOD), catalase (CAT), malondialdehyde (MDA). Astaxanthin (ASTA) partially alleviated the toxicities induced by FCF. These findings suggest that FCF may induce skeletal and neurological toxicities by affecting oxidative stress, disrupting the normal expression of skeletal and nervous system-related genes in the Notch signaling pathway, and ultimately leading to behavioral abnormalities. Our findings may provide new insights into a comprehensive evaluation of FCF toxicology in aquatic organisms, and may assist the government in formulating and implementing regulatory policies regarding the application of FCF.
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INTERNATIONAL JOURNAL OF PHARMACEUTICS
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2026
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IF 5.2
Yuzhe Wang,Lu sheng,Lin Bu,Huijuan Li,Jianxin Wu,Qing Huang
Phospholipid vesicle-based permeation assay (PVPA) is widely applied as in vitro skin alternative model by mimicking stratum corneum to evaluate the transdermal application of drugs and chemicals. However, it is composed of lipid components only, which suffer from limitations of achieving accurate permeability prediction. To remedy this shortcoming, inactive immortalized human keratinocytes (HaCaT) were integrated as component to construct epidermal-mimicking model: EpiPVPA. The EpiPVPA could tolerate the pH 3–10 as well as up to 30% ethanol and was stable for 2 weeks at 4 ℃. And the permeation evaluation by Franz diffusion test of 14 drugs demonstrated that EpiPVPA model is comparable to porcine skin with strong correlation. After quantitative structure–property relationship (QSPR) analysis of physicochemical descriptors of 14 drugs, HLB, TPSA, and log P were selected as main factors applied to build multiple linear regression (MLR) equation, and the corresponding linear correlation R 2 was elevated to 0.9010. EpiPVPA model is feasible to be applied to permeation evaluation of drugs and cosmetics.
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Advanced Healthcare Materials
·
2026
·
IF 11
Si-Yi Li,Ying-Jie Zhu,Heng Li,Han-Ping Yu
The high stability of storage and high mineralization efficiency are pivotal for the restoration of acid-etched dental enamel, yet these demands are mutually exclusive and difficult to be met. Herein, we propose a bioinspired “dormancy-to-activation” mineralization strategy by synthesizing “dormant” adenosine 5’-triphosphate (ATP)-stabilized amorphous calcium phosphate (ACP) nanoparticles as the precursor that can be activated and accelerated by saliva for rapid crystallization to form hierarchically ordered crystalline hydroxyapatite nanorods (mineralization). To realize this goal, we have designed and constructed a bioinspired dormant-ATP-stabilized ACP nanoparticle system with high stability and controllable mineralization ability using ATP biomolecules as the stabilizer. The ACP-ATP nanoparticles can maintain the dormancy for mineralization in vitro, which can stabilize the amorphous structure of ACP nanoparticles for 25 days in water. In the oral environment, the alkaline phosphatase (ALP) in saliva acts as a biological signal to activate and accelerate the mineralization of ACP-ATP amorphous nanoparticles. More importantly, the coating of ACP-ATP amorphous nanoparticles on the acid-etched dental enamel can effectively restore the hierarchically ordered structure, recover mechanical properties, and replicate fracture-resistance behaviors of natural dental enamel.
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Frontiers in Pharmacology
·
2026
·
IF 5.4
Xinyu Li,Xiaoyang Zhou,Zhiyong Li,Zilu Zhu,Songtao Wang,Jiaolong Huang,Kai Lian,Peng Duan,Chunhui Hu,Yihua Shi
Background Tanshinone IIA (TSN), a compound extracted from Salvia miltiorrhiza , has demonstrated a range of pharmacological activities. However, its therapeutic efficacy in glucocorticoid-induced osteoporosis (GIOP) remains insufficiently understood. This study was performed to investigate the protective effects of TSN against prednisolone (PN)-induced osteoporosis in zebrafish larvae. Methods The osteoprotective properties of TSN were assessed using alizarin red S staining, calcein staining, and alcian blue staining to evaluate bone mineralization, density, and cartilage development. Cardiovascular function and locomotor behavior were analyzed using transgenic zebrafish models and behavioral tracking systems. RNA sequencing was performed to identify differentially expressed genes and key signaling pathways. Protein-protein interaction networks were constructed to elucidate gene associations, and results were validated via quantitative reverse transcription polymerase chain reaction. Results TSN administration effectively attenuated PN-induced developmental toxicity, enhanced bone mineralization and density, and improved cartilage abnormalities in zebrafish larvae. Transcriptomic analysis identified 505 genes with reversed expression profiles between the TSN and PN treatment groups, primarily associated with skeletal system development, lipid metabolism, and fatty acid β-oxidation. Key affected pathways included PPAR signaling, lipid atherosclerosis, and reactive oxygen species. Notably, skeletal development was characterized by the upregulation of genes including col1a1b , col2a1b , col9a3 , rdh1 , and acana . Conclusion These findings demonstrate the osteoprotective effects of TSN in mitigating glucocorticoid-induced bone loss and cartilage abnormalities, highlighting its potential as a therapeutic agent for GIOP. The transcriptomic insights provide novel perspectives on TSN’s role in regulating bone metabolism and skeletal development.
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Biomaterials Advances
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2026
·
IF 6.7
Changxu Wang,Shenghao Shi,Fei Wang,Chong Yuan,Di Liu,Yifei Liu
Osteoporosis is a prevalent metabolic bone disorder characterized by an imbalance between bone resorption and formation. Current therapeutic options are constrained by side effects and low bioavailability. Icariin (ICA), a naturally derived osteotropic flavonoid, exhibits osteogenic and anti-osteoclastogenic properties; however, its clinical application is limited due to poor solubility and low oral bioavailability. This study introduces β-cyclodextrin-modified gold nanoclusters (CGNCs) as a nanocarrier for efficient delivery of ICA. The synthesized ICA-loaded CGNCs (ICA-CGNCs) have a hydrodynamic diameter of approximately 2.16 nm, demonstrating excellent dispersity, sustained release kinetics, and storage stability. In vitro, ICA-CGNCs showed good cytocompatibility in both MC3T3-E1 pre-osteoblasts and RAW264.7 macrophages. In MC3T3-E1 cells, ICA-CGNCs promoted osteogenic differentiation, as indicated by increased ALP activity, enhanced mineralization, and upregulated osteogenic genes (ALP, BMP2, RUNX2, and COL1A1 ); immunofluorescence further showed elevated nuclear β-catenin and RUNX2 signals, supporting the involvement of osteogenic pathway-associated events in this cell model. In RAW264.7 cells, ICA-CGNCs suppressed RANKL-induced osteoclastogenesis by reducing TRAP-positive multinucleated osteoclast formation, disrupting F-actin ring organization, and downregulating osteoclast markers ( TRAP, CTSK, MMP9, and NFATc1 ), which was supported by decreased bone resorption pit formation and attenuated nuclear accumulation of NF-κB p65 and NFATc1·In a glucocorticoid-induced zebrafish osteoporosis model, ICA-CGNCs effectively promoted cranial and vertebral bone mineralization without systemic toxicity, outperforming both free ICA and CGNCs alone. This study establishes the ICA-CGNC platform as a dual-action nanotherapeutic strategy with significant potential for osteoporosis treatment and bone regeneration.
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ACS Nano
·
2023
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IF 17.1
Lang Chen,Chenyan Yu,Wanting Xu,Yuan Xiong,Peng Cheng,Ze Lin,Zhenhe Zhang,Leonard Knoedler,Adriana C. Panayi,Samuel Knoedler,Junqing Wang,Bobin Mi,Guohui Liu
Ongoing research has highlighted the significance of the cross-play of macrophages and mesenchymal stem cells (MSCs). Lysine-specific demethylase 6B (KDM6B) has been shown to control osteogenic differentiation of MSCs by depleting trimethylated histone 3 lysine 27 (H3K27me3). However, to date, the role of KDM6B in bone marrow-derived macrophages (BMDMs) remains controversial. Here, a chromatin immunoprecipitation assay (ChIP) proved that KDM6B derived from osteogenic-induced BMSCs could bind to the promoter region of BMDMs’ brain and muscle aryl hydrocarbon receptor nuclear translocator-like protein-1 (BMAL1) gene in a coculture system and activate BMAL1. Transcriptome sequencing and experiments in vitro showed that the overexpression of BMAL1 in BMDM could inhibit the TLR2/NF-κB signaling pathway, reduce pyroptosis, and decrease the M1/M2 ratio, thereby promoting osteogenic differentiation of BMSCs. Furthermore, bone and macrophage dual-targeted GSK-J4 (KDM6B inhibitor)-loaded nanodiscs were synthesized via binding SDSSD-apoA-1 peptide analogs (APA) peptide, which indirectly proved the critical role of KDM6B in osteogenesis in vivo. Overall, we demonstrated that KDM6B serves as a positive circulation trigger during osteogenic differentiation by decreasing the ratio of M1/M2 both in vitro and in vivo. Collectively, these results provide insight into basic research in the field of osteoporosis and bone repair.
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Biomaterials Science
·
2022
·Vol. 11
·期 5
·1714-1724
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IF 7.59
Suping Jiang,Wen Wang,Jiming Ke,Shan Huang,Jie Wang,Chengxi Luo,Xiaoxia Li,Kaili Zhang,Huanhuan Liu,Wensheng Zheng,Jiwen Zhang,Can Peng
Acute gout arthritis (AGA) remains the fundamental research focus in the entire medical field. Hydrogel microneedles (HMNs) loaded with therapeutic molecules such as colchicine (Col) have been developed as a new tool for the management of AGA in a minimally invasive manner. However, the incompatible mechanical and swelling properties of HMNs limited the diffusion of the drug from the HMN system, which remains a challenge for practical use. Here, a mechanically tough (11.53 N per needle) and super-swelling (2708%) hydrogel microneedle (HMNs) composed of a uniform network structure was developed using a UV-responsive crosslinker through in situ photopolymerization for percutaneous delivery of Col. Such HMNs and Col loaded HMNs (Col-HMNs) present excellent biocompatibility. Moreover, Col-HMNs present considerable anti-inflammatory effects in vivo through down-regulated inflammatory responses such as related cytokines IL-1β, IL-6, and TNF-α. These results demonstrated that the mechanically tough and super-swelling HMNs could be a promising tool for effective Col delivery to relieve AGA.
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Advanced Fiber Materials
·
2022
·Vol. 4
·期 4
·894-907
·
IF 12.958
Huang Yiqian,Du Zhiyun,Li Ke,Jing Wei,Wei Pengfei,Zhao Bo,Yu Yingjie,Cai Qing,Yang Xiaoping
Bone defects are always accompanied by inflammation due to excessive reactive oxygen species (ROS) in injured regions, which greatly impedes the regeneration of bone tissues. Although many conductive polymers have been developed to scavenge ROS, they are typically non-degradable under physiological conditions, making them unsuitable for in vivo applications. Biodegradable polyorganophosphazenes (POPPs) may serve as potent ROS-scavenging biomaterials owing to their versatile chemical structures and ease of functionalization. Herein, a PATGP-type electroactive polyphosphazene with side groups of aniline tetramer and glycine ethyl ester was compared to conventional poly(lactic-co-glycolic acid) (PLGA) in regenerating bone tissues. To conduct in vitro and in vivo evaluations, three kinds of electrospun nanofibrous meshes were prepared: PLGA, PLGA/PATGP blend, and PLGA/PATGP core–shell nanofibers. Among them, PLGA/PATGP core–shell nanofibers outperform the blend and PLGA nanofibers in terms of scavenging ROS, promoting osteogenic differentiation, and accelerating neo-bone formation. The continuous PATGP shell on the PLGA/PATGP core–shell nanofiber surface could apparently provide more significant modulation effects on cellular behaviors than the PLGA/PATGP blend nanofibers with PATGP dispersed in the PLGA matrix. Therefore, the core–shell structured PLGA/PATGP nanofibers were envisioned as a promising candidate scaffold for bone tissue engineering. Additionally, the core–shell design paved the way for biomedical applications of functional POPPs in combination with other polymeric biomaterials, without phase separation or difficulty of increasing the molecular weights of POPPs. Graphical abstract
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Soft Matter
·
2022
·Vol. 18
·期 10
·2072-2081
·
IF 4.046
Feixue Gong,Na Du,Wanguo Hou
We recently found that rough glass surfaces (RGSs) can in situ mediate the micelle-to-vesicle transition in single-component solutions of simple single-tailed amphiphiles (STAs), but only result in a relatively small number of vesicles coexisting with a large number of micelles. In the current work, a dehydration–rehydration (DHRH) method was used to induce the formation of vesicles in the single-component aqueous solutions of alkyltrimethylammonium bromides (CnTABs, n = 12, 14, and 16), a kind of typical cationic STAs. That is, a CnTAB micelle solution dropped on smooth glass surfaces (SGSs) was first dried, and the dried CnTAB aggregates were then rehydrated in a monomer solution of CnTAB. A large population of vesicles and even pure vesicle (or vesicle-dominated) systems were obtained, indicating that the DHRH process could more effectively induce the formation of STA vesicles than RGS in situ mediation. The so-obtained vesicles were characterized using DLS, FF-/cryo-TEM, AFM, SAXS, and fluorescence techniques, and their stability was determined. In addition, the effects of the conditions of DHRH and the chain length of CnTABs on the vesicle formation were examined. It was demonstrated that the vesicles can be formed as long as the concentrations of CnTABs in the rehydrated systems are higher than their critical micelle concentrations. The size and wall thickness of vesicles increase with an increase in chain length. A possible mechanism for the DHRH-induced vesicle formation is proposed: bilayer sheets are formed on SGSs during dehydration, and then detached from the SGSs to form vesicles during rehydration. A highly interdigitated structure of alkyl chains between two leaflets was identified in the bilayers, which probably is the origin of the formation and stability of STA vesicles.
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CHEMICAL ENGINEERING JOURNAL
·
2022
·
IF 16.744
Chang Shu,Zhenzhen Zhang,Xiaojun Li,Jiajia Xu,Mengqi Pan,Yadong Chen,Yuqing Lei,Zhiyong Li,Qiaojie Luo,Xiaodong Li
Durability problem of dentin bonding originated from defective bonding interfaces caused by inadequate infiltration of adhesives into demineralized dentin matrix (DDM) is one of the biggest chanllenges in clinical dentistry, while the scientific mechanism remains unclear. We propose that due to poor physical compatibility, the DDM hydrogel-like interface formed by phosphorylated non-collagenous-proteins can be the primary cause of inadequate infiltration of adhesive containing high-content hydrophobic marcromonomers and the formation of numerous defects in the DDM-cured adhesive interface phase. To test this assumption, we develop a novel bonding strategy based on the hydrogel-like interface dehydrogelation refactoring via actively inducing in situ formation of numerous amorphous calcium phosphate nanoparticles within 30 s. The easy-to-collapse DDM is remolded into a robust porous hybrid structure of low polarity. Irrespective of using a wet or dry DDM, the improved physical compatibility between the DDM interface and adhesives greatly promotes infiltration of various adhesives and the integration of the DDM-cured adhesive interface phase. The resulting defect-low bonding interface greatly improves bonding strength and stability indicating the central role of this long-neglected hydrogel-like DDM interface in the problems associated with dentin bonding, which enriches the theory of adhesive dentistry. Free from hard-to-get advanced adhesive materials and sophisticated operations, accessibility and homogeneity of high-quality dentin bonding may benefit clinical dentistry.
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Frontiers in Bioengineering and Biotechnology
·
2022
·
IF 6.064
Xinhui Wang,Yuan Ma,Xingtang Niu,Ting Su,Xiaoqi Huang,Feng Lu,Qiang Chang
Current wound dressing based on hydrogel offers a promising way to accelerate the healing process, yet great challenges remain in the development of a highly integrated and efficient platform with the combination of therapeutic biomolecules and stem cells. Herein, a natural hydrogel wound dressing from egg white can be conveniently obtained by feasible physical crosslinking, the prepared hydrogel dressing features interconnected microporous channels, direct 3D printing, cytocompatibility, and intrinsic biomolecules to advance cell behavior. The 3D printed egg white hydrogels promote the adhesion and proliferation of adipose-derived stem cells (ASCs) without obvious cytotoxicity. In addition, this integrated hydrogel platform accompanied with adipose-derived stem cells accelerates wound healing through the enhancement of fibroblast proliferation, angiogenesis, and collagen rearrangement in the wound bed. The egg white hydrogel provides an effective wound caring product possessing low cost, easy availability along with ready manufacturing, and advanced therapeutic effect, which may be extended for the management of chronic or other complicated wounds.
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危险品化学品经营许可证（带存储）