Colloid and Interface Science Communications
·
2020
·
IF 2.831
Yan Fang Cheng,Yi Hong Mei,Gnanasekar Sathishkumar,Zhi Song Lu,Chang Ming Li,Feng Wang,Qing You Xia,Li Qun Xu
Silk sericin (SS), a by-product in the field of sericulture, is degummed from raw silk and mostly discarded in silk processing wastewater, causing water pollution. However, it could be interesting to explore the possible utilization of hydrophilic SS as a value-added product in the antifouling application. In this work, natural tannic acid (TA) and SS were conjugated via hydrogen bonding interactions, and the resulting TA/SS conjugates were deposited on the titanium (Ti) surfaces through surface adhesive trihydroxyphenyl groups in TA. The current coating strategy avoids the usage of toxic reagents and reactants and represents an eco-friendly way to fabricate antifouling coatings. The successful co-deposition of TA and SS on the Ti surfaces were confirmed by the static contact angle, X-ray photoelectron spectroscopy (XPS), and surface zeta potential measurements. The TA/SS co-deposited Ti (Ti-TA/SS) surfaces show good protein repellent as well as platelet and bacterial anti-adhesive properties. In addition, the Ti-TA/SS surfaces exhibit low cytotoxicity towards L929 mouse fibroblast cells.
研究领域:
生物材料
·
医学工程
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DOI
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
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2016
·
IF 3.902
Bin’en Nie,Haiyong Ao,Teng Long,Jianliang Zhou,Tingting Tang,Bing Yue
Bacitracin immobilized on the titanium (Ti) surface significantly improves anti-bacterial activity and biocompatibility in vitro . In the current study, we investigated the biologic performance (bactericidal effect and bone-implant integration) of bacitracin-modified Ti in vivo . A rat osteomyelitis model with femoral medullary cavity placement of Ti rods was employed to analyze the prophylactic effect of bacitracin-modified Ti (Ti-BC). Thirty-six female Sprague Dawley (SD) rats were used to establish the Ti implant-associated infection. The Ti and Ti-BC rods were incubated with and without Staphylococcus aureus to mimic the contaminated Ti rod and were implanted into the medullary cavity of the left femur, and sterile Ti rods were used as the blank control. After 3 weeks, the bone pathology was evaluated using X-ray and micro-computed tomography (micro-CT) analysis. For the investigation of the Ti-BC implant osseointegration in vivo , fifteen SD rats were divided into three groups (N = 5), namely Ti, Ti-dopamine immobilized (Ti-DOPA), and Ti-BC. Ti rods were implanted into the left femoral cavity and micro-CT and histological evaluation was conducted after 12 weeks. The in vivo study indicated that Ti-immobilized bacitracin owned the prophylaxis potential for the infection associated with the Ti implants and allowed for the osseointegration. Thus, the multiple biofunctionalized Ti implants could be realized via immobilization of bacitracin, making them promising candidates for preventing the Ti implant-associated infections while retaining the osseointegration effects.
研究领域:
生物材料学
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医学工程
·
微生物学
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产品关联
DOI
PubMed
高影响因子
REACTIVE & FUNCTIONAL POLYMERS
·
2019
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IF 3.074
Shuangshuang Wang,Jixuan Song,Yuchao Li,Xingchuan Zhao,Lin Chen,Guang Li,Liping Wang,Zhengfeng Jia,Xiangcai Ge
An environmentally benign and convenient approach to impart titanium (Ti) surfaces with antibacterial functionalities was described in this work. Polydopamine (PDA) was first deposited onto an alkali activated Ti substrates. Then 2-bromoisobutyryl bromide (BIBB) was immobilized on the surface of PDA coated Ti. Finally, the BIBB functionalized Ti initiated the polymerization of N, N -diethylaminoethyl methacrylate (DEAEMA) and N, N -dimethylaminoethyl methacrylate (DMAEMA) to obtain Ti@PDA-g-polymer brushes by activators regenerated by electron transfer atom transfer radical polymerization (ARGET-ATRP). The prepared PDEAEMA and PDMAEMA brushes on Ti surfaces would be subsequently quaternized by using 1-bromooctane, which would impart the obtained products with quaternary ammonium groups. The surface compositions of the functionalized Ti were characterized by X-ray photoelectron spectroscopy (XPS). The hydrophilic/hydrophobic properties of the functionalized Ti surfaces were determined by static water contact angle measurements, and morphologies were characterized by scanning electron microscope (SEM). Antibacterial abilities of quaternized PDEAEMA and PDMAEMA coated Ti were evaluated by the contact killing test, in which live/dead cell staining method was employed. The results showed that the quaternized polymer decorated Ti exhibited good bactericidal effects against Escherichi coli ( E. coli ), and its inhibition rate of E. coli were observed to be nearly 90%.
研究领域:
材料科学
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生物医学工程
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产品关联
DOI
高影响因子
MATERIALS TECHNOLOGY
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2015
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IF 1.227
T. He,W. Zhu,X. Wang,P. Yu,S. Wang,G. Tan,C. Ning
Copper(II) were immobilised onto medical titanium surface by polydopamine (PDA) chelating, to build intrinsic antibacterial implant materials for inhibition of implant infection. A scanning electrical microscope showed that the modified titanium surface morphology was a porous nanostructure. An energy dispersive spectrometer showed the existence of Cu, and X-ray photoelectron spectroscopy results further confirmed that the immobilised copper element was in the form of Cu(II). Bacteria plate colony counting results showed that modified titanium surface had decent antibacterial property, matching the ion release outcomes. It could be conclude that the PDA assisted Cu(II) immobilisation was an efficient and effective method for antibacterial modification of titanium.
研究领域:
生物医学工程
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材料科学
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产品关联
DOI
高影响因子
Journal of the Mechanical Behavior of Biomedical Materials
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2024
·
IF 3.3
Qiang Li,Shuaishuai Li,Hao Sun,Mitsuo Niinomi,Takayoshi Nakano
Iodine-containing coatings were prepared on pure Ti surfaces via electrochemical deposition to enhance their antibacterial properties . The factors influencing iodine content were analyzed using an orthogonal experiment. The electrochemically deposited samples were characterized using scanning electron microscopy with energy dispersive spectroscopy and X-ray photoelectron spectroscopy, and their antibacterial properties and cytotoxicity were evaluated. The results showed that changing the deposition time is an effective way to control the iodine content. The iodine content, coating thickness, and adhesion of the samples increased with deposition time. Iodine in the coatings mainly exists in three forms, which are I 2 , I 3 − , and pentavalent iodine. For samples with iodine-containing coatings, the antibacterial ratios against E. coli and S. aureus were greater than 90% and increased with increasing iodine content. Although the samples with iodine-containing coatings showed some inhibition of the proliferation of MC3T3-E1 cells, the cell viabilities were all higher than 80%, suggesting that iodine-containing coatings are biosafe.
研究领域:
材料科学
·
生物医学工程
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产品关联
DOI
PubMed
高影响因子
APPLIED SURFACE SCIENCE
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2018
·
IF 4.439
Conghui Deng,Xinkun Shen,Weihu Yang,Zhong Luo,Pingping Ma,Tingting Shen,Ju Liu,Kaiyong Cai
Surface topography had been identified as a crucial property that affects osseointegration; thus, topographical modification was the most frequently adopted technique in titanium-based implant research. In this study, ethyl cellulose was employed as an additive to construct a zinc-incorporated nano-network layer onto a titanium surface by the sequential treatments of spin-coating, high-temperature calcination , and alkali heat corrosion. SEM results showed that 20 mg/mL of ethyl cellulose was optimal to fabricate a relatively flat porous coating, and the ideal nano-network structures formed by only 4 h of corrosion. Other results of XPS and ICP further proved that zinc ions were successfully incorporated into the final samples (Ti-Zn0.1, Ti-Zn0.3, and Ti-Zn0.4). Moreover, the in vitro cellular ( e.g. , CCK-8, ALP , mineralization) and bacterial assays presented that Ti-Zn0.3 substrates not only had the greatest proliferation and differentiation capacities for osteoblasts but also possessed relatively strong antibacterial abilities for both Escherichia coli and Staphylococcus aureus . This study provided a new way to rapidly construct the pro-osteogenesis and antibacterial nano-network structures on titanium surfaces for orthopedic application.
研究领域:
生物材料学
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医学工程
·
细胞生物学
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产品关联
DOI
高影响因子
International Journal of Nanomedicine
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2018
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IF 4.37
Yong Huang,Xue Shen,Haixia Qiao,Hao Yang,Xuejiao Zhang,Yiyao Liu,Hejie Yang
Background Two frequent problems associated with titanium (Ti) surfaces of bone/dental implants are their corrosion and lack of native tissue integration.Methods Here, we present an anodization-hydrothermal method for coating Ti surfaces with a layer of silicon (Si)- and strontium (Sr)-loaded titania nanotubes (TNs). The Ti surfaces coated with such a layer (Si–Sr-TNs) were characterized with different techniques.Results The results indicate that the Si4+ and Sr2+ ions were evenly incorporated into the TNs and that the Si–Sr-TN layer provides good protection against corrosive media like simulated body fluid. The excellent cytocompatibility of the coating was confirmed in vitro by the significant growth and differentiation of MC3T3-E1 osteoblastic cells.Conclusion Being easily and economically fabricated, the Si–Sr-TN surfaces may find their niche in clinical applications, thanks to their excellent biological activity and corrosion resistance.
研究领域:
生物材料学
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医学工程
·
组织工程
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产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
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2018
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IF 3.997
Tingting Yang,Donghui Wang,Xuanyong Liu
Titanium and its alloys are widely used in many clinical applications, but implant-associated infection may lead to implant failure. Because of the increasing concern about antibiotic resistant pathogen, photothermal therapy (PTT) as a new treatment strategy has received considerable attention. In this work, gold nanorods (GNRs) photoexcited by the near-infrared (NIR) light were immobilized on Ti surface by electrostatic surface self-assembly technique. Field emission scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy (XPS) were used to explore the morphology and composition of the GNRs-modified surface. The photothermal temperature of the immobilized GNRs was measured by an infrared thermal imaging system in real time. In vitro study reveal that the prepared GNRs-modified surface exhibits antibacterial activity against four kinds of bacterial strains including both Gram-negative bacilli ( Escherichia coli and Pseudomonas aeruginosa ) and Gram-positive cocci ( Staphylococcus aureus and Staphylococcus epidermidis ) under the irradiation of 808 nm laser. Besides, the antibacterial efficiency of the GNRs-modified surface could keep stable after multiple laser exposure. It should be noted that the GNRs-modified surface shows better antibacterial effect against Gram-negative bacilli compared to Gram-positive cocci. Moreover, the GNRs-modified surface has no obvious adverse effect to the osteoblast precursor cells under NIR irradiation. These data demonstrate that the GNRs-modified surface with negligible cytotoxicity and recyclable antibacterial effect provides a favorable model for the translation of photothermal therapy to the clinical application.
研究领域:
生物医学工程
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材料科学
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产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2017
·Vol. 7
·期 53
·33606-33613
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IF 3.108
Liming Wang,Beibei Zhou,Xiaoxiao Huang,Lingqing Dong,Kui Cheng,Wenjian Weng
Alkali treatment has been widely used for the surface modification of Ti and Ti alloys for clinical applications. However, the mechanism underlying the effects of the alkali-treated Ti surface on the cell response still needs to be explored. In this study, we demonstrated that the cell responses on a H2Ti3O7 nanowire film, normally titanate generated on a Ti surface via alkali treatment and ion exchange, were sensitive to the surface hydroxyl groups of the H2Ti3O7 nanowire films, i.e. the total amount as well as comparative ratio of the distinct type of surface hydroxyl groups (bridging-OH and terminal-OH). The surface hydroxyl groups of H2Ti3O7 nanowires were further controlled via heat treatment to produce anatase TiO2 nanowire films. Although there was no difference in both topographies, cells on a H2Ti3O7 nanowire film showed more elongated shape than those on an anatase nanowire film. Moreover, the expression of ALP of cells on H2Ti3O7 upregulated as compared to that on the anatase nanowire film at day 4, suggesting enhanced osteogenic capacity at an early stage. These results could be attributed to the difference in the distinct ratio of the terminal hydroxyl groups to the bridging hydroxyl groups, which was 0.42 for the H2Ti3O7 nanowire film and 0.75 for the anatase nanowire film. It appeared that the bridging hydroxyl groups on H2Ti3O7 were efficient in attracting Ca2+, which influenced the cell morphology and further upregulated the early differentiation.
研究领域:
生物材料学
·
细胞生物学
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DOI
高影响因子
DENTAL MATERIALS JOURNAL
·
2025
·
IF 1.9
Ting YANG,Wenjie HU,Kwok-Hung CHUNG,Fuhua YANG,Hua YANG
Sandblasted with large grits and acid-etched (SLA) surface topography has been a common method of implant surface modification to promote bone-implant integration in titanium-based dental implant manufacturing. However, the acid etching protocols used are various. The aim of this investigation was to validate the effect of different acid-etched treatments of sandblasted titanium surfaces on enhancing osteogenic potential. Five different titanium surface groups were prepared and tested including: i) smooth (Control), ii) sandblasted (SL), iii) sandblasted and acid-etched with H2SO4/HCl mixed solution (SLA/SOCl), iv) sandblasted and acid-etched with H2SO4 solution (SLA/SO), and v) sandblasted and acid-etched with HF solution (SLA/HF). The surface characterization, protein adsorption, and cell behavior were examined to determine the osteogenic potential. Compared with the control group, other surface treated groups showed rougher surface, better wettability, more protein absorption, and cell viability, especially in the SLA/SOCl surface group. The results indicated that SLA surfaces prepared with H2SO4/HCl mixed acid solution provided significant potential to promote osteogenesis.
研究领域:
生物材料学
·
口腔医学
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产品关联
DOI
PubMed
高影响因子
Journal of Physical Chemistry C
·
2022
·
IF 4.177
Xin-Ran Li,Xian-Ze Meng,Qin-Hao Zhang,Hao-Ran Cai,Zhou-Zhou Yan,Lian-Kui Wu,Fa-He Cao
The apparent cathodic current on the Ti surface in acidic solution is composed of three reactions: the reductions of the oxide film, H+, and O2. However, classical electrochemical tests usually provide coupled multi-reaction currents. Furthermore, dissolved oxygen makes in situ measurements significant due to the thermodynamic instability of Ti. In this study, the effects of pre-reduction and dissolved oxygen on hydrogen evolution reaction (HER) kinetics on the Ti surface were investigated by electrochemical and composition characterizations. The HER current was quantitatively separated from the apparent cathodic current from Ti, including the reductions of the oxide film, H+, and O2 by scanning electrochemical microscopy. The HER transfer coefficient and standard rate constant for Ti spontaneously passivated in air (P-Ti) are both 0.48/4.9 × 10–12 cm/s in aerated and O2-saturated solutions, while those for Ti activated by electro-reduction (EA-Ti) are 0.25/3.6 × 10–7 cm/s and 0.25/3.1 × 10–7 cm/s, respectively. The variable HER behavior is caused by the changes in the film electronic structure and composition except for the competition reduction between O2 and H+ as indicated by Mott–Schottky and surface-enhanced Raman spectroscopy. The deviation from 0.5 in the transfer coefficient implies that the Tafel slope and HER mechanism should be analyzed with caution.
研究领域:
材料科学
·
电化学
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产品关联
DOI
高影响因子
SURFACE & COATINGS TECHNOLOGY
·
2024
·
IF 5.3
Qixiang Zhan,Shuhua Deng,Jiayi He,Jinhua Xu,Anfu Chen,Jiajun Luo,Wenjie Zhang,Caihong Lei
The elasticity mismatch between Ti and tissue limits the performance of Ti medical devices. How to create a coating with mimicking natural soft tissue stiffness and possessing strong mechanical bond is a challenge in implant manufacturing. Here, we developed a combined coating, that is, an anodized Ti surface (ATS) with nanostructures coated with a layer of PAAm hydrogel with tunable elasticity. Due to the nano-mechanical interlocking and hydrogen bonding synergy, the PAAm hydrogel layer was tightly anchored in nanostructures on the ATS. By regulating the oxidation voltage, nanostructures including nanopores, nanotubes, and punch-through nanotubes were fabricated on the ATS, and these three kinds of anodized nanostructures increase the porosity of the ATS sequentially. The lap shear test has shown that the shear strength increases linearly with increasing the porosity, and the shear strength of the punch-through nanotube structures with the PAAm hydrogel coating reaches 59.28 kPa. The adhesion mechanism between the anodized Ti nanostructures and the PAAm hydrogel coating is mainly due to the nano-mechanical interlocking and hydrogen bonding synergy, which was proven by morphology analysis, XRD, and ATR-FTIR characterization of the samples subjected to lap shear load. The hydrogel-nanostructures coating has demonstrated the potential to be applied in Ti medical devices.
研究领域:
生物材料
·
材料科学
·
组织工程
·
机械工程
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DOI
高影响因子
危险品化学品经营许可证（带存储）