Yun Wang,Puling Li,Xue Zhang,Lingyun Li,Mengjiao Liu,Xiaoqing Li,Yejia Dai,Cun Zhang,Shaojing Li
The processing method for Chinese traditional herbal medicine is “Pao Zhi” in Chinese. This study examined the efficacy of the Pao Zhi on the preparations of Gardeniae Fructus (GF) on a mitochondrial respiratory function in rats. To determine the efficacy of Pao Zhi, we investigated the effects of GF heat processing on mitochondrial respiratory function. To test the GF components, the rats were randomly divided into a geniposide-alone group, crocin-alone group, and combination groups and treated with geniposide and crocin at different ratios. The results showed that a high dose, raw GF was more effective in improving the neurological function, mitochondrial respiratory function, and activities of Na+-K+-ATPase and Ca2+-Mg2+-ATPase than the preparations that underwent heating. Moreover, mitochondrial ROS production was the lowest in the raw GF-treated group. In addition, treatments with crocin and GC3 were more effective than geniposide in improving the functional deficit in MCAO rats. In conclusion, our results suggest that raw GF is the most suitable preparation for the treatment of cerebral ischemia, and its underlying mechanisms may be associated with the improvement of mitochondrial respiratory function, increased activities of Na+-K+-ATPase and Ca2+-Mg2+-ATPase, and reduced oxidative stress in mitochondria. Our findings suggest that raw GF, especially crocin, could be an ideal therapeutic agent for ischemic stroke.
Wei Liang,Zhang Weimei,Yuan Chen,Jiachen Sun,Fengxia Guo,Jing Hu,Wenyuan Gao,Xia Li
In this work, we developed a novel HPLC method for the simultaneous determination of 16 bioactive components in rhubarb and used it to determine the contents. Through the determination of bioactive components and in vitro activity, we compared the differences between 18 batches of different varieties of rhubarb. The contents of 16 bioactive components in Rheum officinale Baill., Rheum tanguticum Maxim. ex Balf., and Rheum palmatum Linn. were 57.53–76.09, 98.88–130.61 and 36.18–48.66 mg/g, respectively. The average inhibition rates of alkaline phosphatase in R. tanguticum were the highest, reaching 79.53%. The average inhibition rates of thrombin and Na + /K + -ATPase in R. palmatum were the highest, reaching 80.93 and 62.42%, respectively. These results showed that there were significant differences in effective components and biological activities among different varieties of rhubarb. Through chemometric analysis, we preliminarily compared the differences in chemical composition of different varieties of rhubarb, and established a Fisher discriminant function which could accurately distinguish different varieties of rhubarb. Through correlation analysis and molecular docking, three potential components as quality markers of rhubarb were identified. The obtained results provide a comprehensive strategy for the quality control of multisource Chinese medicine and related products.
危险品化学品经营许可证（带存储）