Environmental Science-Processes & Impacts
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2021
·Vol. 23
·期 5
·735-744
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IF 4.238
Yanghui Xiong,Yuan-Bo Wang,De-Sheng Pei
There are two popular methods to measure reactive phosphorus (P) and total P: the GB11893-89 method in China and the 4500-P E method of the APHA in the US. However, their drawbacks, including long reaction time and narrow linear range, cannot satisfy the demand for modern analytic techniques. Here, based on reducing the acidity of the molybdenum blue (MB) reaction, and then stabilizing with EDTA, we have developed a novel method (WH-flags) to quickly measure both reactive P and total P. In the WH-flags method, “W” stands for Wide linear ranges (0–1.4 mg L−1 for reactive P & 0–1.8 mg L−1 for total P), “H” stands for High tolerance of different matrices (it can be adapted to a wide spectrum of samples), “f” is fast reaction time (2 min), “l” is long stabilization (40 min), “a” stands for appropriate recovery rates (89.1–103.2%), “g” is good precision and accuracy (CVs < 5% & Res < 5%) and “s” is small volume of samples (5–10 mL). The WH-flags method showed good linearity (R2 > 0.999). The WH-flags method should have broad application in monitoring P from different aqueous samples in the future.
研究领域:
环境科学
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分析化学
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产品关联
DOI
PubMed
高影响因子
INORGANIC CHEMISTRY
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2024
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IF 4.3
Hui-Min Zeng,Mengyuan Cheng,Weimin Xuan,Yongge Wei
Cyclic compounds are appealing owing to their intrinsic porous structures and facile accessibility as building blocks (BBs) for fabricating high-order assemblies. Nevertheless, the modular synthesis of such molecular entities and their subsequent controlled assembly are still very challenging. Herein, we report the synthesis of a gigantic molybdenum blue (MB) wheel {Mo158} (1), featuring a skirt-shaped structure dimerized from {Mo79}. {Mo79} exhibits an unprecedented octameric architecture built from eight sets of {Mo8} BBs, and the controlled assembly of this smallest member in the MB library is achieved by proper wheel contraction induced by {Mo1} and edge-sharing {Mo2} BBs. Moreover, {Mo158} can function as a 4-connected giant synthon, connecting with adjacent four clusters in a controlled manner via four Mo–O–Mo bridges, resulting in a high-order architecture with a 2D layer structure. In addition to single-crystal X-ray diffraction, {Mo158} is fully characterized by a variety of spectroscopies, allowing for the unambiguous determination of its structure and formula. This work not only enriches the family of gigantic clusters but also demonstrates their potential for constructing more complex assemblies.
研究领域:
无机化学
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材料科学
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产品关联
DOI
PubMed
高影响因子
Nanoscale Horizons
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2025
·
IF 6.6
Baoru Fang,Siqi Geng,Ke Wang,Fang Wang,Yiqing Zhou,Jiaying Qin,Shengnan Luo,Yanping Chen,Zhangsen Yu
Photothermal therapy (PTT) stands as an emerging and promising treatment modality and is being developed for the treatment of breast cancer, prostate cancer, and a series of superficial tumors. This innovative approach harnesses photothermal agents (PTAs) that convert near-infrared light (NIR) energy into heat, efficiently heating and ablating localized lesion tissue. Notably, the low scattering of NIR-II (1000–1500 nm) band light within biological tissue ensures superior penetration depth, surpassing that of NIR I (700–900 nm) band light. Consequently, developing PTAs with excellent absorption performance and biocompatibility in the NIR-II band has attracted significant attention in photothermal therapy research. We successfully synthesized phosphomolybdenum blue (PMB) nanoparticles using single-strand DNA (ssDNA) as a template in this innovative study. Subsequently, we delved into this material's absorption characteristics and photothermal properties across the NIR-I and NIR-II spectral regions. Furthermore, we evaluated the therapeutic efficacy of PMB on 4T1 cells and tumor-bearing mouse models of breast cancer. Our findings revealed that PMB not only exhibits remarkable biocompatibility but also possesses stellar photothermal performance. Specifically, under 808 nm and 1064 nm laser irradiation, PMB achieved photothermal conversion efficiencies of 21.37% and 28.84%, respectively. Notably, compared to 808 nm laser irradiation, even when transmitting through a 2 mm thick tumor tissue homogenate, the 1064 nm laser irradiation maintained a robust tumor ablation effect. What's more, PMB possesses critical pH-responsive degradation properties. For instance, PMB nanoparticles degrade rapidly under physiological conditions (pH 7.2–7.4) while degrading slower in the acidic tumor microenvironment (pH 6.0–6.9). This unique characteristic significantly mitigates the systemic toxicity of PMB and enhances the safety of photothermal therapy implementation. Moreover, our study represents the first instance of utilizing ssDNA as a template for synthesizing a PMB nano photothermal agent and demonstrating its exceptional tumor thermal ablation efficacy. This groundbreaking work offers novel insights into the development of safe, efficient, and pH-responsive photothermal agents for cancer therapy.
研究领域:
生物医学工程
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肿瘤学
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纳米医学
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DOI
PubMed
高影响因子
Journal of Environmental Sciences
·
2024
·
IF 5.9
Xiaobao Tuo,Yanhua Duan,Guanting Lin,Tianci Jiang,Wenhui Liu,Fangyi Chen,Xianjun Xie,Yan Zheng
Rapid screening of inorganic arsenic (iAs) in groundwater used for drinking by hundreds of millions of mostly rural residents worldwide is crucial for health protection. Most commercial field test kits are based on the Gutzeit reaction that uses mercury-based reagents for color development, an environmental concern that increasingly limits its utilization. This study further improves the molybdenum blue (MB) colorimetric method to allow for faster screening with more stable reagents. More importantly, a portable three-channel colorimeter is developed for screening iAs relative to the WHO drinking water guideline value (10 µg/L). Adding the reducing reagents in sequence not only prolongs the storage time to > 7 days, but also accelerates the color development time to 6 min in conjunction with lowering the H 2 SO 4 concentration in chromogenic reagents. The optimal pH ranges from 1.2 to 1.3 and is achieved by acidifying groundwater to 1% ( V/V ) HCl. With detection limits of 3.7 µg/L for inorganic arsenate (iAs(V)) and 3.8 µg/L for inorganic arsenite (iAs(III)), testing groundwater with ∼10 µg/L of As has a precision < 20%. The method works well for a range of phosphate concentrations of 48–950 µg/L (0.5–10 µmol/L). Concentrations of total_iAs (6–300 µg/L), iAs(V) (6–230 µg/L) and iAs(III) (0–170 µg/L) for 14 groundwater samples from Yinchuan Plain, Pearl River Delta, and Jianghan Plain, are in excellent agreements (linear regression slope: 0.969–1.029) with the benchmark methods. The improved chemistry here lays the foundation for the MB colorimetric method to become a commercially viable screening tool, with further engineering and design improvement of the colorimeter.
研究领域:
环境科学
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公共卫生
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL BIOCHEMISTRY
·
2023
·
IF 2.9
Jiawang Xiang,Bing Zhang,Yani Shi,Yanfei Wen,Yuan Yuan,Jianying Lin,Zhihuan Zhao,Jing Li,Yan Cheng
As the most well-known analytical tool, the thermometer has been extended to the field of biological analysis based on the photothermal effect. Herein, isoniazide modified Ag nanoparticles were prepared as nanolabels to build an immunoassay . The nanoparticles were characterized by transmission electron microscope (TEM), dynamic laser scattering (DLS), X-ray powder diffraction (XRD), and Fourier transform infrared (FT-IR). When the target protein was present, the sandwich immunoassay was developed and the photothermal reaction was triggered by isoniazide modified Ag nanoparticles. As a reducing agent, isoniazide is used to transform phosphomolybdic acid hydrate into molybdenum blue solution. And molybdenum blue had good photothermal stability and high photothermal conversion efficiency. The temperature variation of molybdenum blue solution showed a positive correlation with the concentration of carcinoembryonic antigen (CEA). Thus, the target protein of CEA was quantitative detection by thermometer. The linear response range is 0.1 ng mL −1 to 40 ng mL −1 , and the detection limit is 0.08 ng mL −1 . Moreover, the proposed protocol had satisfactory selectivity, accuracy, and reproducibility.
研究领域:
纳米技术
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生物医学工程
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分析化学
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癌症研究
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产品关联
DOI
PubMed
高影响因子
TALANTA
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2022
·
IF 6.556
Yanfei Wen,Yuan Yuan,Bing Zhang,Jianying Lin,Zhihuan Zhao,Jing Li,Yan Cheng
A photothermal immunoassay was built for tumor marker detection based on Ag 4 P 2 O 7 @Ag nanocomposites. Ag 4 P 2 O 7 @Ag nanomaterials were synthesized by precipitation-photoreduction reaction, and characterized by transmission electron microscope (TEM), scanning electron microscopy (SEM), atomic force microscopy (AFM), X-ray photoelectron spectra (XPS) and X-ray powder diffraction (XRD). Come about PO 4 3− derived from Ag 4 P 2 O 7 @Ag under acidic conditions react with ammonium molybdate in the action of reductant generating molybdenum blue. The photothermal change is due to molybdenum blue solution depending on the concentration of carcinoembryonic antigen (CEA) in immunoassay. Under optimal conditions, there is a linear relation between ΔT and CEA concentration in the range of 1 pg mL −1 –40 ng mL −1 with the detection limit of 0.33 pg mL −1 . Meanwhile, the developed photothermal immunoassay displays preferable selectivity , repeatability, and stability.
研究领域:
分析化学
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纳米技术
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癌症研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2025
·
IF 2.6
Zhaoyang Gu,Yongming Huang,Beichen Lin,Kunde Lin
Current methods for determining dissolved reactive phosphorus (DRP) in sediment porewater mainly rely on ex-situ analysis, which can be labor-intensive and introduces errors due to changes in the concentration and morphology during sampling and transportation. Therefore, there is a clear need for in-situ measurement methods for DRP in sediment porewater. In this study, a novel in-situ analytical method was developed by combining diffusive gradients in thin-films (DGT) and digital imaging techniques, based on the classic phosphomolybdenum blue (PMB) method. During deployment, DRP in sediment porewater diffuses into the DGT device, while reducing reagent (ascorbic acid) from the diffusive gel simultaneously diffuses into the binding gel and reacts with ammonium molybdate, resulting in the formation of PMB compounds. The color of the PMB compounds was recorded in-situ using a digital imaging technique, and the resulting images were analyzed to calculate the concentration of DRP in surface sediment porewater. By using the new method, analytical data of DRP in surface sediment porewater could be quickly obtained in the field near real-time, without water sampling and any further sample treatment, transportation and instrumental analysis in laboratories. The key parameters including the loading procedure for colorimetric reagents in the gels, the concentrations of ammonium molybdate and ascorbic acid, the amount of C18, and the thickness of the C18 binding gel, were systematically optimized. The optimized method demonstrated a wide linear range (4 ‒ 1000 μmol L-1, R2=0.993), with satisfactory accuracy (-6.23% ‒ 8.68%, n = 3) and good precision (6.60% ‒ 13.46%, n = 3) at an exposure time of 60 min. The method also performed well across different salinities (0 ‒ 35) and in the presence of interfering ions (SiO32‒, AsO33‒, F‒, and S2‒). The method was successfully applied to both freshwater and seawater sediments, with results comparable to those obtained using traditional analytical methods. Overall, the proposed method provides a new strategy for in-situ determination of DRP in surface sediment porewater.
研究领域:
环境分析化学
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海洋与淡水沉积物分析
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原位传感技术
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比色检测
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地球化学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）