Journal of Oceanology and Limnology
·
2024
·
IF 1.3
Ma Xiao,Wang Xueli,Zhou Shaoqi,Ma Jianrong,Wang Jingfu,Chen Jingan,Zeng Yan,Chen Qiao,Qin Boqiang,Li Ming
Genus Microcystis is the most common dominant species of bloom-forming cyanobacteria. Growth and colony formation are critical processes for the occurrence of Microcystis blooms. To identify allelochemicals that can inhibit the growth and colony formation of Microcystis, Microcystis aeruginosa was cultivated and treated with various concentrations of gramine, catechol, berberine, and sanguinarine in laboratory. Results indicate that the growth, colony formation, and extracellular polysaccharides (EPS) content of Microcystis were significantly inhibited when treated with gramine, catechol, and berberine in appropriate concentrations at high iron level (6.8 mg/L). Microcystis growth was inhibited and colony formation and EPS content were promoted when treated with sanguinarine at high iron level. These findings suggest that cyanobacterial blooms can be controlled by regulating the input of gramine, catechol, and berberine. Furthermore, this study revealed that plant allelochemicals could affect the growth and morphology of algae in aquatic ecological restoration, which may be potentially important for improving water quality.
研究领域:
水生生态学
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环境科学
✓ 已验证
DOI
高影响因子
Crop & Pasture Science
·
2019
·Vol. 70
·期 4
·359-366
·
IF 1.33
Xiaoli Tan,Xin Yang,Yinan Xie,Han Xiao,Mengjiao Liu,Lianghuan Wu
We studied the relative efficacy of different forms of foliar iron (Fe) fertilisation on leaf re-greening in Fe-deficient, purple-fleshed sweet potato (Ipomoea batatas (L.) Lam.) varieties xuzi8 and xuzi6. Activities of ferric chelate reductase (FCR) and concentrations of Fe were measured in the leaves and roots at intervals over 5 days to quantify recovery from leaf chlorosis. Freshly expanded and chlorotic leaves were immersed in one of three different fertiliser compounds containing 9 mm Fe: FeSO4, Fe2(SO4)3, Fe(III)-EDTA. An Fe-sufficient treatment and an Fe-deficient control were included. The experiment had a completely randomised block design with five replications per treatment and was conducted in a sunlit glasshouse. For variety xuzi8, leaf FCR activity in the Fe2(SO4)3 treatment was highest at 1 h after application, and higher than all other treatments, whereas FeSO4 and Fe(III)-EDTA treatments showed their highest FCR at day 5 after application, both significantly higher than the Fe2(SO4)3 and control treatments. Furthermore, leaf Fe concentration reached a maximum in the FeSO4 treatment at day 1, and in the Fe2(SO4)3 treatment at day 3. By contrast, root Fe concentration was relatively constant and lower in the foliar Fe treatments than the Fe-sufficient and -deficient treatments. For variety xuzi6, leaf SPAD was higher with the Fe2(SO4)3 than the FeSO4 treatment at day 5 after application. In general, FCR activity and Fe concentrations in roots and leaves of xuzi6 were higher than those of xuzi8. Variations in leaf Fe concentrations were similar for both the FeSO4 and Fe2(SO4)3 treatments of the two varieties. Maximum leaf Fe levels in xuzi6 were ~4-fold those in xuzi8. The results of the study suggest that foliar-applied Fe2(SO4)3 was the most effective compound at correcting Fe-deficiency symptoms. The higher leaf and root FCR activity and Fe concentration in xuzi6 might explain its higher tolerance to Fe deficiency and better re-greening than xuzi8.
研究领域:
植物学
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营养学
✓ 已验证
DOI
高影响因子
JOURNAL OF GEOPHYSICAL RESEARCH-PLANETS
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2020
·Vol. 125
·期 12
·e2020JE006590
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IF 3.717
Peixin Du,Peng Yuan,Jiacheng Liu,Yixuan Yang,Hongling Bu,Shun Wang,Junming Zhou,Hongzhe Song,Dong Liu,Joseph R. Michalski,Chengshuai Liu
Allophane, a common component on Earth and a probable constituent of the amorphous component on Mars, is closely associated with Fe in the form of structural Fe and/or iron (oxyhydr)oxide coatings. However, until now, the formation and evolution of allophane as products of environmental Fe concentrations have rarely been studied. We investigated allophane precipitation from gels with different Fe/(Fe + Al) molar ratios ( n , 0 ≤ n ≤ 1.0). X-ray diffraction patterns and Fourier transform infrared spectra of the products showed that allophane was nearly the only product at n ≤ 0.2 and that the crystallinity of Fe-rich allophane decreased with increasing n . Combined with the results of transmission electron microscopy and Mössbauer spectroscopy, Fe was found to be mainly incorporated into the gibbsite-like sheet of allophane, forming clusters; the highest Fe-for-Al substitution content was roughly estimated to be 20 mol.%. As n increased further, the formation of allophane was increasingly suppressed, and the Fe phases began to separate from the Al-Si phases, resulting in mixtures of incipient allophane and incipient akaganeite and finally akaganeite. The near-infrared spectroscopic data (1.2–2.6 μ m) of the products showed incapability in identifying poorly ordered minerals in Al-Si-Fe systems, while the features in the range of 0.4–1.2 μ m were powerful for studying iron occurrence in the products. These findings not only offer insights into the formation, evolution and geological role of allophane in Al-Si-Fe systems on Earth but also help constrain the paleoenvironment of locations where allophane and akaganeite co-occur on Mars. Key Points Environmental Fe concentrations control the formation and evolution of allophane in Al-Si-Fe systems Near-infrared spectra (1.2–2.6 μ m) show incapability in identifying short-range ordered minerals in Al-Si-Fe systems Co-occurrence of allophane and akaganeite would constrain the local palaeoenvironment on Mars
研究领域:
地质学
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行星科学
✓ 已验证
DOI
高影响因子
JOURNAL OF CEREAL SCIENCE
·
2023
·
IF 3.8
Wenzhe Gao,Zhiyu Zhou,Kai Wang,Xingshu Wang,Zikang Guo,Chao Li,Zhaohui Wang,Mei Shi
Information on the distribution and concentration of iron (Fe) in different grain tissues in staple crops is required to alleviate the problem of human iron deficiency . A new method was established to quantitatively assay the Fe concentration and distribution in wheat grain tissues by the chromatic values obtained from images of stained grain transections, and variations of grain tissue Fe concentration and distribution were analyzed for different wheat cultivars and over sites in the present study. Obtained results showed that concentration of Fe was higher in aleurone and scutellum, and lower in endosperm of wheat grains. The concentration of Fe in grain tissues had significant differences among cultivars and over sites, especially in the crease aleurone layer and endosperm between the two experimental regions. The Fe in the scutellum, crease aleurone layer, and endosperm were found to be the main contributors to the whole grain Fe in the North of China, and in the South that was the endosperm, scutellum, crease and the aleurone layer. In conclusion, this research established a new method for the quantitative determination of Fe concentration and distribution in wheat grain tissues and provided information for improving the agronomic measures and wheat breeding for biofortification of Fe.
研究领域:
作物生理学
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植物营养学
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农业生物技术
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食品科学
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微量元素代谢
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分子生物学
✓ 已验证
DOI
高影响因子
Pharmaceuticals
·
2022
·Vol. 15
·期 10
·1177
·
IF 5.215
Yini Dang,Qing He,Siyu Yang,Huaiqing Sun,Yin Liu,Wanting Li,Yi Tang,Yu Zheng,Ting Wu
Objectives: Despite significant advances in neuroscience, the mechanisms of AD are not fully understood. Single-cell RNA sequencing (scRNA-seq) techniques provide potential solutions to analyze cellular composition of complex brain tissue and explore cellular and molecular biological mechanisms of AD. Methods: We investigated cellular heterogeneity in AD via utilization of bioinformatic analysis of scRNA-seq in AD patients and healthy controls from the Gene Expression Omnibus (GEO) database. The “GOplot” package was applied to explore possible biological processes in oligodendrocytes, astrocytes, and oligodendrocyte progenitor cells (OPCs). Expression patterns and biological functions of differentially expressed genes (DEGs) from scRNA-seq data were validated in RNA sequencing data. DEGs in astrocytes interacted with ferroptosis-related genes in FerrDb. CCK-8 and EdU assays were performed to measure cell proliferation ability. ROS, Fe2+level, mitochondrial membrane potentials, iron concentrations, and total iron binding capacity (TIBC) in serum were evaluated. Y-maze and elevated maze were used to measure anxiety-like behavior. Autonomous and exploration behaviors or learning and memory ability in mice were analyzed using open field test and novel object recognition test. Results: Multiple clusters were identified, including oligodendrocytes, astrocytes, OPCs, neurons, microglia, doublets, and endothelial cells. Astrocytes were significantly decreased in AD, while oligodendrocytes and OPCs increased. Cell-to-cell ligand–receptor interaction analysis revealed that astrocytes, neurons, and OPCs mainly established contacts with other cells via the NRG3–ERBB4 ligand–receptor pair. GO and KEGG analyses found that astrocytes were enriched in the ferroptosis pathway. FTH1 and SAT1 in astrocytes were identified as hub mRNAs associated with ferroptosis. Serum iron concentration of 5xFAD mice was higher than that of WT, and emotional and cognitive function were significantly impaired as compared to WT. Serum iron concentration was negatively correlated with number of astrocytes and percentage of time spent entering the novelty arm in the Y-maze test, while it was positively correlated with percentage of time spent in the central area. Meanwhile, number of astrocytes was negatively correlated with percentage of time spent in the central area, while it was positively correlated with percentage of time spent entering the novelty arm. Conclusions: Through scRNA-seq analysis, we found that ferroptosis was activated in astrocytes and may contribute to the pathophysiological process in the entorhinal cortex. FTH1 and SAT1 were identified to impact astrocyte ferroptosis. Emotional and cognitive impairment in AD was associated with astrocyte ferroptosis. Our findings provide clues to reveal the pathophysiological processes following AD at the cellular level and highlight potential drug targets for the treatment of AD.Keywords:Alzheimer’s disease;single-cell RNA sequencing;astrocytes;ferroptosis
研究领域:
神经科学
·
分子生物学
·
生物信息学
·
神经退行性疾病
✓ 已验证
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Jing Jing,Zhijun Zhang,Zhiyong Hu,Xuemei Ma,Jianlan Cui,Hailin Zhu,Xueli Bai,Lijun Zhai
Iron deficiency anemia (IDA) is the most common nutritional disease worldwide. In this study, a low methoxyl pectin (LMP)‑iron(III) complex was prepared. The physicochemical and structural properties were characterized by HPSEC, HPIC, CV, FTIR, 1 H NMR, XRD, SEM and CD. The results showed that iron increased the molecular weight of the LMP‑iron(III) from 11.50 ± 0.32 to 12.70 ± 0.45 kDa and improved its crystallinity. Moreover, the findings demonstrated that -OH and -COOH groups in LMP coordinate with Fe 3+ to form β -FeOOH. The water-holding capacity, emulsion stability, and antioxidant activities of the LMP‑iron(III) were lower than those of LMP. Furthermore, the therapeutic effects of LMP‑iron(III) on IDA were investigated in rats. Following LMP‑iron(III) supplementation, compared with the model group, the administration of LMP‑iron(III) significantly increased the body weight, hemoglobin concentration, and serum iron concentration as well as decreased free erythrocyte protoporphyrin concentration. Therefore, the LMP‑iron(III) can potentially treat IDA in rats experiments, providing a theoretical basis for the development of a promising iron supplement.
研究领域:
营养学
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生物化学
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药学
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动物科学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）