JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2018
·
IF 5.091
Xiaoli Huang,Di Lu,Yongmin Ma,Limei Zhang,Lina Wang,Jian Deng,Zheng Wang,Yanjun Zhao
We report a self-assembly-based approach for improved iron chelation of small chelators. A model chelator, deferiprone (DFP) is covalently linked to amphiphilic poly(ethylene glycol)-polypeptide block copolymer that can assemble into micelles . The free DFP generally coordinates with iron in a ratiometric manner (3:1). However, the assembly induces DFP packing in the micelle cores, which restricts its conformational freedom. Hence DFP/iron coordination ratio in micelles is reduced (< 3:1), which leads to an enhanced chelation performance of DFP. In addition, the micellar nanocarrier usually exhibits an extended systemic circulation in contrast to the free DFP. The current work opens new avenues for developing novel nanomedicines for iron overload diseases.
研究领域:
生物化学
·
药学
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2018
·
IF 4.946
Leqi Cui,Jing Fan,Yifei Sun,Zhenbao Zhu,Jianhua Yi
Salts reduction/substitution have gained a lot interest from food industry since the US Food and Drug Administration (FDA) has issued a draft guidance for salt reduction. However how changes of salts in food formulation could influence lipid oxidation is still not fully understood. Using oil-in-water emulsions stabilized by a natural emulsifier – lecithin at pH 7.0 as a model system, this study evaluated how salts affect the physical parameters of the emulsion, the chelating activity of lecithin and thus the lipid oxidation of these emulsions. Results showed that salts increased the particle size, the negative charge of the oil droplets, and the amount of iron chelated by lecithin. Lipid oxidation lag phases were shortened by addition of salts, by 1 day and 2 days for lipid hydroperoxides and thiobarbituric acid reactive substances measurements respectively. These results provide some new insights on the mechanisms of how salts could affect the lipid oxidation of food emulsions.
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
·
2024
·
IF 2.6
Shuaishuai Wei,Jiaying Huo,Jia Lin,Ziwei Zhang,Shijian Dong,Lulu Ma,Shugang Li
Summary In this study, a method to produce iron-chelating peptides derived from egg yolk proteins was developed and optimised. The egg yolk proteins were enzymolysed and ultrafiltered to prepare egg yolk peptide (EYP), and then the EYP was chelated with Fe to produce egg yolk peptide-Fe (EYP-Fe). The optimised mass ratio of peptide to iron, pH, temperature and reaction time were found at 8:1, pH 8.0, 40 °C and 30 min, respectively. The mean particle size of EYP-Fe was 389.2 ± 2.61 nm, and the chelating ratio was 95.38 ± 1.93%. The measurements of FTIR, X-ray photoelectron spectroscopy (XPS) and NMR showed that ferrous ions mainly combined with the amino and carboxyl of EYP to form EYP-Fe. In vitro stability studies have shown that the iron release rate of the EYP-Fe is maintained at around 5% under alkaline conditions, and there is no significant difference in iron release rate under conditions of 30 °C–80 °C; besides, it has a lower iron release rate (18%) in gastrointestinal digestion compared to FeSO 4 (97.93%), indicating that the EYP-Fe has good heat tolerance, alkalinity and digestion tolerance. The above results provide a scientific basis for the use of EYP-Fe derived from egg yolk protein as a potential alternative to iron supplements.
研究领域:
食品科学
·
营养学
·
化学
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产品关联
DOI
高影响因子
Advanced Healthcare Materials
·
2024
·
IF 10
Tian-Qiu Xie,Xiao Yan,Jian-Hua Yan,Yun-Jian Yu,Xin-Hua Liu,Jun Feng,Chuan-Jun Liu,Xian-Zheng Zhang
Bacteria, especially drug-resistant strains, can quickly cause wound infections, leading to delayed healing and fatal risk in clinics. With the growing need for alternative antibacterial approaches that rely less on antibiotics or eliminate their use altogether, a novel antibacterial hydrogel named Ovtgel is developed. Ovtgel is formulated by chemically crosslinking thiol-modified ovotransferrin (Ovt), a member of the transferrin family found in egg white, with olefin-modified agarose through thiol–ene click chemistry. Ovt is designed to sequester ferric ions essential for bacterial survival and protect wound tissues from damages caused by the reactive oxygen species (ROS) generated in Fenton reactions. Experimental data have shown that Ovtgel significantly enhances wound healing by inhibiting bacterial growth and shielding tissues from ROS-induced harms. Unlike traditional antibiotics, Ovtgel targets essential trace elements required for bacterial survival in the host environment, preventing the development of drug resistance in pathogenic bacteria. Ovtgel exhibits excellent biocompatibility due to the homology of Ovt to mammalian transferrin. This hydrogel has the potential to serve as an effective antibiotic-free solution for combating bacterial infections.
研究领域:
生物材料学
·
微生物学
·
医学工程
✓ 已验证
DOI
PubMed
高影响因子
Advanced Healthcare Materials
·
2025
·
IF 9.6
Yong Xu,Xin Gu,Xingchen Li,Yicheng Chen,Zhenyuan Wei,Jielin Wang,Yi Liu,Yunhan Ji,Qian Cheng,Shuai Jiang,Jiangming Yu,Xiaoxiao Li,Wenguo Cui,XiaoJian Ye
Excessive intracellular iron accumulation can induce mitochondrial dysfunction, leading to chondrocyte ferroptosis, a key contributor to cartilage damage in osteoarthritis (OA). Here, micelle-microfluidic hydrogel microspheres, featuring keto-enol-thiol bridged nano-sized secondary structures that disintegrate within the intracellular peroxidative environment to reveal β-diketone groups with metal chelation capabilities, are utilized for the in situ removal of reactive iron, thereby facilitating cartilage repair through the restoration of mitochondrial homeostasis. The relevant experiments demonstrate that the microspheres reduce iron influx by downregulating transferrin receptor (TfR1) expression and decrease mitochondrial iron uptake by upregulating mitochondrial outer membrane iron-sulfur cluster protein (CISD1), thus restoring intracellular mitochondrial iron homeostasis. Furthermore, the antioxidant properties of the ketone-thioether segments synergistically mitigate chondrocyte phospholipid peroxidation via Nrf2/SLC7A11/GPX4 axis, inhibiting ferroptosis and slowing OA progression. In summary, this system that in situ sustainably chelates reactive iron via metal coordination exhibits great potential in the minimally invasive treatment of OA and other ferroptosis-mediated diseases.
研究领域:
生物材料学
·
医学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2021
·
IF 4.952
Shanting Lin,Xiao Hu,Laihao Li,Xianqing Yang,Shengjun Chen,Yanyan Wu,Shaoling Yang
Tilapia skin collagen was hydrolyzed by five proteases (trypsin, pepsin, neutral protease, alkaline protease and protamex). The results showed that the tilapia skin collagen hydrolysate (TSCH) obtained by 2 h hydrolysis with trypsin exhibited the highest iron chelating rate. The TSCH was then separated by immobilized metal affinity chromatography (IMAC-Fe 2+ ) and obtained the tilapia skin collagen iron-chelating peptides (TSCICP). The iron chelating sites of TSCICP were corresponding to carboxylic groups of Asp/Glu and guanidine nitrogen of Arg/Lys. After chelated with iron ions , TSCICP was folded and aggregated to form spherical particles with increased particle size. The TSCICP-iron complexes could maintain high iron solubility at various pH and in simulated gastrointestinal digestion. The iron bio-accessibility of TSCICP-iron complexes was high than that of ferrous glycinate and ferrous sulfate. Finally, TSCICP was purified by RP-HPLC and identified by LC-MS/MS. Four iron-chelating peptides were identified as GPAGPAGEK (782.39 Da), DGPSGPKGDR (984.46 Da), GLPGPSGEEGKR (1198.59 Da) and DGPSGPKGDRGETGL (1441.68 Da). These results indicating that the iron-chelating peptides derived from tilapia skin collagen could be used as potential dietary iron supplement.
研究领域:
食品科学
·
营养学
✓ 已验证
产品关联
DOI
高影响因子
Food Bioscience
·
2023
·
IF 5.2
Xiang-Rong Cheng,Yu-Yao Chen,Cong-Cong Cao,Jia-Hui Ma,Chen-Xi Zhang,Zi-Wei Zhao,Qian Wu,Yuan-Yuan Li,Mitchell N. Muskat,Guo-Qing Wu
Colla Corii Asini (Ejiao) peptide-iron (EPI) chelates formed during digestion have been revealed as potential hematopoietic components. However, it remains unclear how EPI regulates endogenous metabolism in vivo. Iron deficiency anemia (IDA) mice were gavaged with EPI chelates in a gradient dosage, while Ejiao hydrolysate-iron (EJI) chelates and FeSO 4 were used as positive controls. The effect of EPI on hematopoietic recovery was evaluated. For the plasma untargeted metabolomic analysis, liquid chromatography-tandem mass spectrometry (LC-MS/MS) were applied to identify metabolites in the samples. EPI significantly increased the levels of hemogram in IDA mice in a dose-dependent manner. The relative iron bioavailability in EPI was 123.45% of that in FeSO 4 in vivo . After high dose of EPI (EPI-H) supplementation, the levels of 22 potential metabolic markers in the IDA group returned to normal, including sphingomyelin and arachidonic acid . EPI-H affected endogenous metabolites by regulating the metabolism of energy, lipids , and amino acids , promoted hematopoiesis , regulated metabolic pathways, and improved iron homeostasis . It’s a comprehensive nutritional intervention of IDA by EPI supplementation.
研究领域:
营养学
·
代谢组学
·
血液学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Bin Jiang,Hongshen Yue,Xinhao Fu,Jiaming Wang,Yu Feng,Dongmei Li,Chunhong Liu,Zhibiao Feng
Prolyl endopeptidase from Aspergillus niger (An-PEP) is an enzyme that recognizes C-terminal peptide bonds of amino acid chains and cleaves them by hydrolysis . An aqueous two-phase system (ATPS) was used to separate An-PEP from fermentation broth. Through single factor experiments, the ATPS containing 16 % ( w/w ) PEG2000 and 15 % ( w/w ) (NH 4 ) 2 SO 4 at pH 6.0 obtained the recovery of 79.74 ± 0.16 % and the purification coefficient of 7.64 ± 0.08. It was then used to produce soy protein isolate peptide (SPIP) by hydrolysis of soy protein isolate (SPI), and SPIP-Ferrous chelate (SPIP-Fe) was prepared with SPIP and Fe 2+ . The chelation conditions were optimized by RSM , as the chelation time was 30 min, chelation temperature was 25 °C, SPIP mass to VC mass was two to one and pH was 6.0. The obtained chelation rate was 82.56 ± 2.30 %. The change in the structures and functional features of SPIP before and after chelation were investigated. The FTIR and UV–Vis results indicated that the chelation of Fe 2+ and SPIP depended mainly on the formation of amide bonds. The fluorescence, SEM and amino acid composition analysis results indicated that Fe 2+ could induce and stabilize the surface conformation and change the amino acid distribution on the surfaces of SPIP. The chelation of SPIP and Fe 2+ resulted in the enhancement of radical scavenging activities and ACE inhibitory activities. This work provided a new perspective for the further development of peptide-Fe chelates for iron supplement.
研究领域:
生物化学
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOORGANIC CHEMISTRY
·
2020
·
IF 4.831
Jianan Guo,Zhisheng Mi,Xiaoying Jiang,Changjun Zhang,Zili Guo,Linzi Li,Jinping Gu,Tao Zhou,Renren Bai,Yuanyuan Xie
A series of active hybrids combining 3-hydroxypyridin-4(1 H )-one and coumarin pharmacophores were designed and synthesized as potential agents for the treatment of Alzheimer's disease (AD). All the compounds exhibited excellent iron-chelating activities (pFe 3+ = 14.8–19.2) and showed favorable monoamine oxidase B (MAO-B) inhibitory effects compared to the reference drug Pargyline (IC 50 = 86.9 nM). Among them, compound 11 g displayed the best MAO-B inhibitory activity with an IC 50 value of 99.3 nM. Molecular docking analysis showed that compound 11 g could enter the entrance cavity and substrate cavity of MAO-B. Furthermore, the compound 11 g had an excellent antioxidant effect and was capable of protecting from the amyloid- β 1-42 (A β 1-42 ) induced PC12 cell damage. In silico tools were applied for predicting the blood–brain barrier (BBB) penetration and compound 11 g was proved to overcome the brain exposure challenge. In the mice behavioral study, compound 11 g significantly ameliorated cognitive impairment induced by Scopolamine. More importantly, compound 11 g displayed favorable pharmacokinetic profiles in a rat model. In summary, compound 11 g , with both anti-MAO-B and iron-chelating ability, was proved to be a promising potential anti-AD agent for further optimization.
研究领域:
神经科学
·
药物化学
·
生物化学
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产品关联
DOI
PubMed
高影响因子
CHEMISTRY OF NATURAL COMPOUNDS
·
2017
·Vol. 53
·期 2
·248-253
·
IF 0.46
Jing Linlin,Ma Huiping,Fan Pengcheng,Jia Zhengping
Flavones are a group of plant secondary metabolites with multiple biological properties. In the present study, 5,6,7,8-tetrahydroxyflavone (5,6,7,8-THF) was synthesized and characterized. In vitro antioxidant study, the effect of 5,6,7,8-THF on total antioxidant activity, reducing power, DPPH radical scavenging, ABTS radical scavenging, superoxide radical scavenging, hydroxyl radical scavenging, nitric oxide radical scavenging, and ferrous chelating activities was examined. According to the results, 5,6,7,8-THF showed excellent free radical scavenging effect and reducing power but weak the ferrous chelating activity. In conclusion, 5,6,7,8-THF can be regarded as an excellent source of antioxidants.
研究领域:
植物化学
·
药理学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
International Journal of Bioprinting
·
2023
·
IF 8.4
Jianpeng Gao,Hufei Wang,Ming Li,Zhongyang Liu,Junyao Cheng,Xiao Liu,Jianheng Liu,Xing Wang,Licheng Zhang
Intramembranous ossification (IMO) and endochondral ossification (ECO) are two pathways of bone regeneration. The regeneration of most bone, such as limb bone, trunk bone, and skull base bone, mainly occurs in the form of endochondral ossification, which has also become one of the effective ways for bone tissue engineering. In this work, we prepared a well-structured and biocompatible methacrylated gelatin/polymethacrylic acid (GelMA/PMAA) hydrogel by digital light processing (DLP) printing technology, which could effectively chelate iron ions and continuously activate the hypoxia-inducible factor-1 alpha (HIF-1α) signaling pathway to promote the process of endochondral ossification and angiogenesis. The incorporation of PMAA endowed the hydrogel with remarkable viscoelasticity and high efficacy in chelation of iron ions, giving rise to the activation of HIF-1α signaling pathway, improving chondrogenic differentiation in the early stage, and facilitating vascularization in the later stage and bone remodeling. Therefore, the findings have significant implications on DLP printing technology of endochondral osteogenesis induced by the iron-chelating property of biological scaffold, which will provide an effective way in the development of novel bone regeneration.
研究领域:
组织工程
·
生物材料
·
再生医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF INORGANIC BIOCHEMISTRY
·
2017
·
IF 3.348
Qingchun Zhang,Bo Jin,Zhaotao Shi,Xiaofang Wang,Shan Lei,Xingyan Tang,Hua Liang,Qiangqiang Liu,Mei Gong,Rufang Peng
A new tris(dopamine) derivative, containing three dopamine chelate moieties which were attached to a trimesic acid molecular scaffold , has been prepared and fully characterized by NMR , FTIR and HRMS . The solution thermodynamic stability of the chelator with Fe(III), Mg(II), Zn(II) and Fe(II) ions was investigated. Results demonstrated that the chelator exhibited effective binding ability and improved selectivity to Fe(III) ion. The chelator possessed affinity similar to that of diethylenetriaminepentaacetic acid chelator for Fe(III) ion. The high affinity could be attributed to the favorable geometric arrangement between the chelator and Fe(III) ion coordination preference. The chelator also exhibited high antioxidant activity and nontoxicity to neuron-like rat pheochromocytoma cells. Hence, the chelator could be used as chelating agent for iron overload situations without depleting essential metal ions , such as Mg(II) and Zn(II) ions.
研究领域:
药物化学
·
生物无机化学
·
金属离子螯合研究
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）