Wansu Zhang,Shangyu Chen,Pengfei Sun,Shuai Ye,Quli Fan,Jun Song,Pengju Zeng,Junle Qu,Wai-Yeung Wong
The luminescence of traditional phosphorescence-based hypoxia probes is limited to the visible and first near-infrared wavelength regions (<1000 nm), which has defects of higher light scattering and lower penetration depth in contrast with the second near-infrared wavelength window (NIR-II, 1000–1700 nm) for optical bioimaging. Herein, 5,15-bis(2,6-bis(dodecyloxy)phenyl)-porphyrin platinum(II) (PpyPt) with J-aggregation induced NIR-II phosphorescence is reported. J-aggregates of PpyPt are confirmed by the X-ray diffraction data in the crystalline state. Moreover, the emission and excitation spectra of PpyPt in the solid states reveal NIR-II luminescence feature of PpyPt in J-aggregates. More importantly, by preparation of water-soluble PpyPt nanoparticles (PpyPt NPs 4.76 ) with J-aggregates, it has NIR-II phosphorescent lifetime of microseconds and good oxygen-sensitivity in water. Moreover, the good biological hypoxia-sensing potential of PpyPt NPs 4.76 is demonstrated in cells and 4T1-tumor-bearing mice. This study provides an efficient strategy to design NIR-II phosphorescent probe for sensitive tumor-hypoxia detection through the construction of J-aggregates.
Ke Zhang,Lingling Luo,Wenjie Li,Honglin Zhang,Yi Zhang,Jiupeng Zhao,Yao Li
The preparation of polystyrene (PS) beads loaded with platinum octaethylporphyrin (PtOEP) via a swelling method has been well incorporated into dissolved oxygen (DO) sensing detection. The experimental results showed that the highest value of I0/I100 was 7.26 and the loading ratio of PtOEP was 51.33%; the luminescent intensity declined by only 2.23% under UV light for 1 hour. The response and recovery times were 16 s and 180 s, respectively. The peak position and intensity of the emission spectrum in buffer solutions with different pH values did not change. This indicates that this oxygen sensor has excellent sensitivity, light stability, reversibility, and pH resistance. Moreover, on this basis, we incorporated another oxygen insensitive dye, coumarin 6 (C6), to prepare a ratiometric oxygen sensor, which showed high sensitivity towards O2 with superior quenching efficiency. Finally, this oxygen sensor was used to monitor the content of DO in fish tank, tap water, and the Songhua River. All the measured results were well within the reference range.
A glassy carbon electrode (GCE) was modified with a film containing titanium dioxide and a Pt(II)-porphyrin complex, and its response to quercetin was investigated employing cyclic voltammetry and chronoamperometry. The oxidation current caused by quercetin is largely enhanced under UV illumination. The effects of pH value, mass of TiO 2 in the film, UV illumination time and applied potential were studied. Under optimized conditions, the peak current at a typically applied voltage of +0.4 V depends linearly on the concentration of quercetin in the 0.002 to 50 mg L −1 range. The detection limit (at an SNR of 3) is 0.8 μg L −1 . The method was successfully applied to the determination of quercetin in (spiked) samples of tea and apple juice.
Figure Cyclic voltammograms of the TiO 2 /PtP/GCE in the absence and presence of various concentrations of quercetin in 0.1 M NaOH. a-e: 0, 0.1, 0.2, 0.3 and 0.4 mg L -1 .
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