Nanoscale Research Letters
·
2018
·Vol. 13
·期 1
·1-10
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IF 3.125
Wu Fengshou,Yue Liangliang,Su Huifang,Wang Kai,Yang Lixia,Zhu Xunjin
Photosensitizers are light-sensitive molecules that are highly hydrophobic, which poses a challenge to their use for photodynamic therapy. Hence, considerable efforts have been made to develop carriers for the delivery of PSs. Herein, we synthesized a new theranostic nanoagent (CQDs@PtPor) through the electrostatic interaction between the tetraplatinated porphyrin complex (PtPor) and the negatively charged CQDs. The size and morphology of as-prepared CQDs and CQDs@PtPor were characterized by a series of methods, such as XRD, TEM, XPS, and FTIR spectroscopy. The CQDs@PtPor composite integrates the optical properties of CQDs and the anticancer function of porphyrin into a single unit. The spectral results suggested the effective resonance energy transfer from CQDs to PtPor in the CQDs@PtPor composite. Impressively, the CQDs@PtPor composite showed the stronger PDT effect than that of organic molecular PtPor, suggesting that CQDs@PtPor is advantageous over the conventional formulation, attributable to the enhanced efficiency of 1 O 2 production of PtPor by CQDs. Thus, this CQDs-based drug nanocarrier exhibited enhanced tumor-inhibition efficacy as well as low side effects in vitro, showing significant application potential in the cancer therapy.
研究领域:
生物医学
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材料科学
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癌症治疗
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DOI
PubMed
高影响因子
AICHE JOURNAL
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2013
·Vol. 59
·期 8
·2743-2752
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IF 2.493
Pengwei Jin,Ju Chu,Yu Miao,Jun Tan,Siliang Zhang,Weihong Zhu
Microbioreactors with multioptical sensors have become increasingly important because of their small working volumes, high degree of parallelization and the available robotics. A novel hydrophobic luminescent copolymer P(Pt-TPP-TFEMA) along with reference P(Pt-TPP-EMA) containing the pendant group of 5,10,15,20-tetraphenylporphyrin (TPP) moiety as low-cost dissolved oxygen (DO) chemosensor film for high-throughput microbioreactors is designed. Its sensor film exhibits fast response to DO with good stability and fatigue resistance, being capable of applying as a low-cost DO indicator for high-throughput bioprocess measurement. Results show that the quenching response of DO increases with the enhancement in the copolymeric hydrophobicity using the presented hybrid fluorinated ethyl methacrylate. Furthermore, the long emission band at 650 nm of chromophore TPP with large Stoke's shift about 250 nm brings several advantages, such as low scattering, deep penetration, and minimal interferences of absorption and fluorescence from the fermentation system, which shows high-promising application in bioprocess monitoring. © 2013 American Institute of Chemical Engineers AIChE J , 59: 2743–2752, 2013
研究领域:
生物工程
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化学工程
·
光学传感
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DOI
高影响因子
JOURNAL OF LUMINESCENCE
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2019
·
IF 2.961
Si Yao,Li Chen,Fang Jia,Xinzhi Sun,Huifang Su,Hongjian Liu,Lixia Yang,Zejiang Wang,Fengshou Wu,Kai Wang
For a precisely targeted photosensitizer , we designed and prepared a new platinated porphyrin ( Pt-Por-FA ) by Pt-Por tailed with folic acid ( FA ). The intercalation mode of Pt-Por-FA binding to ct-DNA was confirmed by UV–vis and fluorescent spectra. The singlet oxygen generation of Pt-Por-FA was evaluated through 2′, 7′-dichlorofluorescin (DCFH) method. The cellular uptake and the intracellular localization of Pt-Por-FA were studied upon A549 and HeLa cells through confocal laser scanning microscope. The results showed that the red fluorescence of porphyrin conjugate observed in HeLa cells [folate receptor (FR)–positive] was significantly stronger than that of A549 cells (FR-negative), indicating that Pt-Por-FA could target specifically the cancer cells with overexpression of FR via FR-mediated endocytosis. Pt-Por-FA successfully localized in the lysosomes of cancer cells, but Pt-Por without FA did not. Moreover, the cytotoxicity against HeLa and MDA-MB-231 cells was measured by MTT assay . The results demonstrated that Pt-Por-FA had minor cytotoxicity in the dark, but showed a obviously improving toxicity in presence of light. At the concentration of 20 μM Pt-Por-FA , the cell viabilities were only 12.0% and 26.4% with the IC 50 values down to 5 μm and 12 μm against HeLa and MDA-MB-231 cells, respectively. It suggests that Pt-Por-FA could be a precisely targeted photosensitizer with over-expression of a folate receptor.
研究领域:
药理学
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肿瘤学
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生物化学
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DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
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2017
·Vol. 41
·期 13
·5429-5435
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IF 3.269
Yongyun Mao,Qian Zhao,Tingting Pan,Jiayan Shi,Shimei Jiang,Meiwan Chen,Bingpu Zhou,Yanqing Tian
Integrating an oxygen-sensitive probe (OSP) onto a three-dimensional (3D) microstructure substrate is a plausible technique to significantly improve the sensitivity of oxygen sensors. Despite the fact that polydimethylsiloxane (PDMS) matrices are regarded as the ideal sensory support, there are still no reports on an OSP functionalized flexible PDMS-based micropillar array (PDMS-MPAs) film as an optical oxygen sensor with capabilities to efficiently improve the sensor's overall performance, e.g. sensitivity, detection limit and stability against photo-bleaching. Herein, we reported the covalent bonding of a functional copolymer of platinum porphyrin (PtTPP)/3-(trimethoxysily)propylmethacrylate (PtTPP/TPMA) as the OSP on the surface of hydroxyl-modified PDMS-MPAs through a “grafting reaction” between PtTPP/TPMA and PDMS-MPAs. The homogeneous dispersion and well-isolated arrangement of the PtTPP/TPMA OSP on the 3D PDMS-MPA surface can ensure that the grafted OSP efficiently quenches the oxygen molecules. Consequently, the 3D PtTPP/TPMA-PDMS-MPA sensor film gives rise to a high fluorescence quenching efficiency of about 95% at 1 atm O2 (I0/I100 = 23) and 96% in aqueous solution (I0/IO2satu = 16) with an excellent linear Stern–Volmer plot (ksv = 0.1 h Pa−1, R2 = 0.997 in gas atmosphere and ksv = 12.8 mmol−1 L, R2 = 0.999 in aqueous solution, respectively), excellent reversibility and an ultra-low detection limit (4.7 μmol L−1) when compared with state-of-the-art PDMS-based oxygen sensors. Apart from the competitive sensitivity, the highly durable, reusable superiorities of the 3D PtTPP/TPMA-PDMS-MPA film can further facilitate the exploration and realization of the presented strategy for future oxygen sensor applications, such as the characterization of nearly anoxic systems in harsh environments.
研究领域:
材料科学
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生物医学工程
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化学传感
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产品关联
DOI
高影响因子
Journal of Materials Chemistry C
·
2017
·Vol. 5
·期 44
·11395-11402
·
IF 5.256
Yongyun Mao,Qian Zhao,Jianchang Wu,Tingting Pan,Bingpu Zhou,Yanqing Tian
Organic–inorganic hybrid 3-(trimethoxysily)propylmethacrylate-co-platinum porphyrin-co-methacrylolsobutyl-polyhedral oligomeric silsesquioxane (TPMA-PtTPP-POSS) copolymer films were synthesized and applied as high-performance oxygen sensors. High sensitivity and fast response characteristics originate from the POSS-containing copolymer composition and wormlike structures. The wormlike structures composed of the POSS-containing copolymers self-assembled on the film surface can ensure the homogeneous dispersion of PtTPP and can be perturbed by a trace oxygen environment. Compared with the state-of-the-art PtTPP-TPMA film, the TPMA-PtTPP-POSS copolymer sensors exhibit a swift response (approx. 0.6 s), long-term stability, higher sensitivity (increased by 4-fold), and a relatively large dynamic range, etc. We believe that the POSS-containing hybrid films should provide a new strategy for designing sensitive and fast responding optical oxygen sensors.
研究领域:
材料科学
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化学传感器
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产品关联
DOI
高影响因子
ORGANIC & BIOMOLECULAR CHEMISTRY
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2017
·Vol. 15
·期 27
·5764-5771
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IF 3.564
Sizhe Zhu,Si Yao,Fengshou Wu,Lijun Jiang,Ka-Leung Wong,Ji Zhou,Kai Wang
Organelle and nucleus dual-targeted anticancer drugs are being increasingly used for efficient cancer therapy as they can attack the double vital sites of tumor cells. In this work, we synthesized and characterized two new porphyrin compounds Pt-Por-RB and Me-Por-RB. The spectral titration results suggest that both Pt-Por-RB and Me-Por-RB bind to DNA efficiently in an intercalation binding mode. Upon irradiation, Pt-Por-RB with low dark-cytotoxicity can rapidly generate singlet oxygen to damage the tumor cells through the process of photodynamic therapy. Compared with Me-Por-RB, Pt-Por-RB was not only internalized in the organelles, but also in the nuclei of HeLa cells, probably due to the presence of platinum complexes, as analyzed using the confocal laser scanning microscope. Thus, with the combination of organelle and nucleus dual-targeting property and high efficiency of singlet oxygen generation, Pt-Por-RB showed a significant therapeutic activity against tumor cells.
研究领域:
药理学
·
肿瘤学
·
生物化学
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Yongyun Mao,Muhammad Akram,Jiayan Shi,Jiaxing Wen,Cheng Yang,Jiapei Jiang,Zhouguang Lu,Bingpu Zhou,Yanqing Tian
Polymeric microfibers, particularly the fluorinated copolymers’ fibers, are promising functional materials for photoelectric devices, sensing, and energy storage due to their various surface characteristics. However, to obtain the high fluorine content copolymer-based fibers with considerable uniformity is likely to be especially challenging, which seriously debilitates their applications in sensor devices. Herein, for the first time, we presented a high fluorine content platinum porphyrin-grafted poly(isobutyl methacrylate-co-dodecafluoroheptyl methacrylate) copolymers (PtTFPP-p(IBM-co-DFHMA)) microfibrous thin-films used as optical oxygen sensors. The porous thin-film frameworks were formed of uniform microfibers, which afforded an exceptional improvement in sensitivity and exhibited 584% higher sensitivity than the solid sensing film owing to the large specific surface area, porous structures and oxygen diffusion enhancement by fluorine elements. Additionally, the remarkable emission intensity-changing characteristic of the microfiber sensing film under various air pressures facilitates convenient visualization of pressure distributions on film surface. The characteristics are particularly important for the computational fluid dynamics simulations in various sensing fields such as unsteady flow visualization and unsteady pressure measurements, etc. Owing to its attractive advantages and versatile performance, fluorine-containing copolymers fibers are expected to provide a new strategy for the rational design of high performance gas sensor devices.
研究领域:
材料科学
·
传感技术
·
光学工程
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产品关联
DOI
高影响因子
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