SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2025
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IF 4.6
Zongtao Li,Shaodui Ye,Leshou Zhang,Zhenjuan Duan,Shengchun Yang,Yong Liu,Xiaodong Wen
Heavy metal residues in Chinese herbal medicines (CHMs) have raised significant concerns due to their inherent toxicological implications for human health. Therefore, there is an imperative requirement to establish an effective and sensitive method for detecting heavy metal ions. In this study, sulfur quantum dots (SQDs) were fabricated through a sonication-assisted H 2 O 2 etching approach, and the spectral characteristics of SQDs in deep eutectic solvent (DES) micelles were studied. This study found that SQDs significantly aggregate in the long alkyl chains of surfactant-like DES, thereby enhancing the luminescent intensity of SQDs. A fluorescent probe that acts as a “turn-off” was developed using DES-sensitized SQDs to determine Pb 2+ . The detection mechanism of this probe for Pb b+ was further analyzed. Under optimized experimental conditions, the linear range of Pb 2+ concentrations was determined to be 20–100 μM, with a detection limit of 1.61 μM. Validation studies of the method demonstrated that the sensitivity of the established sensor was 1.7-fold greater than that of the bare SQDs probe. Furthermore, the established method was verified using Pb 2+ in Paris polyphylla as the research subject, yielding a satisfactory recovery rate. In short, the DES-sensitized SQDs fluorescent platform is a sensitive, simple, and effective method for detecting Pb 2+ in complex samples.
研究领域:
分析化学
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环境科学
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纳米技术
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药物分析
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生物传感
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产品关联
DOI
PubMed
高影响因子
iScience
·
2023
·
IF 5.8
Han Wang,Dongtian Miao,Yongjiang Yu,Zhihan Zhang,Youlong Zhu,Qing Wang
Lead (Pb(II)) contamination is common in liquid foods and can result from Pb(II) being present in the raw materials or during handling processes. However, due to the complexity of food matrices, there is limited data available concerning Pb(II) ion removal from food sources. This study focused on fabricating a PVA/PAA/DMTD electrospun nanofibrous membrane (ENFM) to efficiently and selectively remove Pb(II) ions from liquid foods. The PVA/PAA/DMTD ENFM had a maximum adsorption capacity of 138.3 mg/g for Pb(II) ions and demonstrated high selectivity toward the removal of Pb(II) ions. Negative values of the Gibbs free energy (Δ G °) showed that the spontaneous nature of the adsorption process was feasible at different temperatures. Moreover, it successfully removed Pb(II) ions from selected samples of commercially available drinks. Therefore, this adsorbent exhibits significant potential for removing Pb(II) ions from liquid food products, thereby reducing daily dietary exposure to Pb(II).
研究领域:
材料科学
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环境科学
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食品科学
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DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2022
·Vol. 189
·期 9
·1-11
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IF 6.408
Zhai Hongguo,Wang Yue,Yin Jiaqi,Zhang Yuhao,Guo Qi,Sun Xia,Guo Yemin,Yang Qingqing,Li Falan,Zhang Yanyan
MXene@Au as the base and Au@SiO 2 as signal amplification factor were used for constructing an ultrasensitive “on–off” electrochemiluminescence (ECL) biosensor for the detection of Pb 2+ in water. The use of MXene@Au composite provided a good interface environment for the loading of tris(2,2-bipyridyl)ruthenium(II) (Ru(bpy) 3 2+ ) on the electrode. Based on resonance energy transfer, the Au (core) SiO 2 (shell) (Au@SiO 2 ) nanoparticles stimulate electron transport and promote tripropylamine (TPrA) oxidation. The luminescence effect of Au@SiO 2 was five times that of AuNPs and SiO 2 nanomaterials alone, and the ECL intensity was greatly improved. In addition, Pb 2+ activated the aptamer to exert its endonuclease activity, which realized the signal cycle amplification in the process of Pb 2+ detection. When Pb 2+ was added, the ECL signal weakened, and the Pb 2+ concentration was detected according to the decreased ECL intensity. Under optimized experimental conditions, this aptamer sensor for Pb 2+ has a wide detection range (0.1 to 1 × 10 6 ng L −1 ) and a low detection limit (0.059 ng L −1 ). The relative standard deviation (RSD) of the sensor is 0.39–0.99%, and the recovery of spiked standard is between 90.00 and 125.70%. The sensor shows good selectivity and high sensitivity in actual water sample analysis. This signal amplification strategy possibly provides a new method for the detection of other heavy metal ions and small molecules. Graphical abstract
研究领域:
分析化学
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环境科学
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材料科学
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生物化学
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DOI
PubMed
高影响因子
Catalysts
·
2020
·Vol. 10
·期 6
·600
·
IF 3.52
Zui Tao,You Zhou,Nuo Duan,Zhouping Wang
Lead (II) is regarded as one of the most hazardous heavy metals, and lead contamination has a serious impact on food chains, human health, and the environment. Herein, a colorimetric aptasensor based on the graphene/Fe3O4-AuNPs composites with enhanced peroxidase-like activity has been developed to monitor lead ions (Pb2+). In short, graphene/Fe3O4-AuNPs were fabricated and acted as an enzyme mimetic, so the color change could be observed by chromogenic reaction. The aptamer of Pb2+was decorated on the surface of the amine magnetic beads by streptavidin–biotin interaction, and the complementary strands of the aptamer and target Pb2+competed for the binding Pb2+aptamer. In the presence of Pb2+, aptamers bonded the metal ions and were removed from the system by magnetic separation; the free cDNA was adsorbed onto the surface of the graphene/Fe3O4-AuNPs composites, thus inhibiting the catalytic activity and the color reaction. The absorbance of the reaction solution at 652 nm had a clear linear correlation with the Pb2+concentration in the range of 1–300 ng/mL, and the limit of detection was 0.63 ng/mL. This assay is simple and convenient in operation, has good selectivity, and has been used to test tap water samples, which proves that it is capable for the routine monitoring of Pb2+.Keywords:peroxidase;aptamer;graphene;enzyme mimetic;colorimetric method;food safety;rapid detection
研究领域:
环境科学
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分析化学
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材料科学
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生物技术
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公共卫生
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产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 8
·1-8
·
IF 5.479
Song Xiaolei,Wang Yu,Liu Su,Zhang Xue,Wang Jingfeng,Wang Haiwang,Zhang Fenfen,Yu Jinghua,Huang Jiadong
An amplified electrochemical biosensing scheme is described for lead(II) ions. It is making use of DNAzyme-assisted target recycling and catalytic hairpin assembly (CHA). The hairpin strand (substrate probe for the Pb 2+ -based DNAzyme; referred to as SP) is composed of trigger probe (TP) and a capture probe 1 attached to gold nanoparticles (AuNP). In the presence of the enzyme probe that partially hybridizes with SP, the introduction of Pb 2+ triggers target recycling and drives the highly amplified translation of target Pb(II) to TP. The CHA reaction is further initiated by TP. The modified AuNP act as the connecting unit, and this leads to the formation of a 3D DNA-AuNP network on the electrode (which is the third amplification step). It can bind the positively charged redox mediator RuHex via electrostatic interaction for electrochemical detection. This biosensor has a low detection limit (95 pM) and any analytical range that covers the 100 pM to 5 μM Pb(II) concentration range. It is selective over other divalent metal ions. It was applied to the determination of Pb 2+ in spiked real-world samples.
Schematic presentation of the electrochemical biosensor. The triply amplified electrochemical assay is based on the use of DNAzyme-assisted target recycling with catalytic hairpin assembly (CHA) reaction for sensitive and selective determination of lead ion (Pb 2+ ). AuNP: gold nanoparticles; SP: substrate probe; EP: enzyme probe.
研究领域:
分析化学
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生物化学
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纳米技术
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环境科学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2021
·
IF 6.953
Yingbi Chen,Jiali Tang,Shixing Wang,Libo Zhang
A chitosan-based adsorbents (CS-Ninhydrin) was prepared by grafting ninhydrin for Pb(II) ions adsorption. SEM-EDS, XRD and FTIR analysis were used to characterize the synthesized CS-Ninhydrin. The static adsorption experiments showed that CS-Ninhydrin had a good removal rate for Pb(II) ions in a wide range of pH 3 to 7, quickly reached equilibrium (120 min) and had a higher adsorption capacity (196 mg/g). Pseudo second-order and Langmuir models showed that the adsorption process of Pb(II) by CS-Ninhydrin was a single-layer chemical adsorption. Temperature experiments showed that the reaction was a spontaneous exothermic process. In the wastewater experiment, CS-Ninhydrin showed an excellent selectivity to Pb(II) ions. The reusability of CS-Ninhydrin was perfect after five adsorption-desorption cycles. The main adsorption mechanism was the chelating and electrostatic action between N and O groups in CS-Ninhydrin and Pb(II) ions. Therefore, the new adsorbent CS-Ninhydrin was expected to promote the wide application of chitosan in Pb(II) adsorption.
研究领域:
环境科学
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材料科学
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化学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）