Nanomaterials
·
2020
·Vol. 10
·期 8
·1531
·
IF 4.324
Qiang Li,Yunhao Li,Heguo Li,Xiaoshan Yan,Guolin Han,Feng Chen,Zhengwei Song,Jianqiao Zhang,Wen Fan,Changfeng Yi,Zushun Xu,Bien Tan,Wei Yan
As one of the widely studied metal nanoclusters, the preparation of copper nanoclusters (Cu NCs) by a facile method with high fluorescence performance has been the interest of researchers. In this paper, a simple, green, clean, and time-saving chemical etching method was used to synthesize water-soluble Cu NCs using ascorbic acid (AA) as the reducing agent. The as-prepared Cu NCs showed strong green fluorescence (with a quantum yield as high as 33.6%) and high ion stability, and good antioxidant activity as well. The resultant Cu NCs were used for the detection of 4-aminoazobenzene (one of 24 kinds of prohibited textile compounds) in water with a minimum detection limit of 1.44 μM, which has good potential for fabric safety monitoring.Keywords:copper nanoclusters;fluorescence;detection;ascorbic acid
研究领域:
化学
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环境科学
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材料科学
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DOI
PubMed
高影响因子
Biosensors-Basel
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2023
·Vol. 13
·期 9
·854
·
IF 5.4
Yang Qing,Haobin Fang,Yuxing Yang,Yazhen Liao,Haiyu Li,Zhencui Wang,Jie Du
At present, a large number of studies have demonstrated that miRNAs can be used as biological indicators for the diagnosis and treatment of diseases such as tumours and cancer, so it is important to develop a new miRNA detection platform. In this work, miRNA-122 is used as the basis for targeting detection agents. We have designed an unlabelled DNA1 that undergoes partial hybridisation and has a 20 T base long strand. The fluorescent signal in this experiment is derived from copper nanoclusters (CuNCs) generated on the circular T-long strand of DNA1. At the same time, DNA1 is able to react with miRNA-122 and achieve hydrolysis of the part bound to miRNA-122 via the action of nucleic acid exonuclease III (Exo III), leaving a part of the DNA, called DNA3, while releasing miRNA-122 to participate in the next reaction, thus achieving circular amplification. DNA3 is able to react with DNA2, which is bound to streptavidin magnetic beads (SIBs) and separated from the reaction solution via the application of a magnetic field. Overall, this is a fluorescence signal reduction experiment, and the strength of the fluorescence signal from the copper nanoclusters can determine whether the target miRNA-122 is present or not. The degree of fluorescence reduction indicates how much DNA1, and thus the amount of target miRNA-122, has been hydrolysed. By evaluating the variations in the fluorescence signal under optimised conditions, we discovered that this method has good sensitivity, with a detection limit as low as 0.46 nM, better than many other previous works on fluorescence signal-based biosensors for miRNA detection. This technique offers high discrimination and selectivity and can serve as a persuasive reference for early diagnosis.Keywords:exonuclease III;miRNA-122;CuNCs;fluorescence reduction
研究领域:
分子生物学
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生物医学工程
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PubMed
高影响因子
Analytical Methods
·
2019
·Vol. 11
·期 28
·3584-3589
·
IF 2.378
Yanqiong Lai,Xia Teng,Yanli Zhang,Hongbin Wang,Pengfei Pang,Chun Yang,Colin J. Barrow,Wenrong Yang
In this study, we developed a simple, sensitive, low-cost and label-free method to detect rutin by using double-stranded DNA-templated copper nanoclusters (dsDNA-CuNCs) as a fluorescent probe. The dsDNA-template can facilitate the formation of highly fluorescent CuNCs with a maximum emission wavelength of 575 nm. In the dsDNA-CuNC fluorescent probe, rutin was intercalated into the structure of dsDNA via hydrogen bonding and electrostatic interactions. The fluorescence of dsDNA-CuNCs was quenched considerably due to the electron transfer from CuNCs to rutin. This decrease in the fluorescence intensity of dsDNA-CuNCs can be used to monitor the concentration of rutin from 0.6 to 400 μM with a detection limit of 0.12 μM (S/N = 3). Compared with the previous reports, this method does not require any complex DNA sequence design, fluorescent dye label and sophisticated experimental techniques. Moreover, the proposed sensing system could be applicable for the detection of rutin in pharmaceutical samples.
研究领域:
生物化学
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分析化学
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药物分析
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DOI
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2025
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IF 5.4
Chong Liu,Zhicheng Wang,Na Zhang,Yue Li,Shulin Li,Xia Xin
The precise synthesis and self-assembly of metal nanoclusters (NCs) featuring customized morphologies and structures, represent a crucial step in the exploration of structure-property relationships and the discovery of new functionalities. This study presents a strategy to induce the self-assembly of copper nanoclusters ( Cu-NCs , Cu 4 I 4 (PPh 3 ) 4 ) into stable and highly fluorescent regular micron level hexagonal plates by nonionic surfactant Tyloxapol and mixed solvent (DMSO/H₂O). The aggregation-induced emission (AIE) behavior markedly enhanced the fluorescence quantum yield and lifetime of Ty-Cu-NCs , from 0.57 % and 68.6 ns to 22.60 % and 2.8 μs, representing a ∼40-fold improvement in both. Kinetic studies of the assembly process have revealed a sequence involving nucleation, aggregation, oriented growth and subsequent size enlargement. When varying the types of surfactants, the formation of Ty-Cu-NCs assembly with different morphologies was observed which indicated the unique role of Tyloxapol in directing the formation of hexagonal plates. It is noteworthy that the Ty-Cu-NCs assembly exhibits high selectivity and sensitivity towards Fe³⁺ ions, with a detection limit of 1.51 μM, which is considerably lower than the maximum permissible concentration of Fe³⁺ in tap water as per health standards. The combination of high fluorescence properties and selective ion detection capabilities renders these Ty-Cu-NCs assembly promising candidates for applications in ion sensing and anti-counterfeiting technologies.
研究领域:
材料科学
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化学
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分析化学
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高影响因子
MICROCHIMICA ACTA
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2019
·Vol. 187
·期 1
·1-9
·
IF 5.479
Shi Yu-e,Han Fei,Xie Luoyuan,Zhang Chuanchuan,Li Tianzi,Wang Henggang,Lai Wing-Fu,Luo Shaojuan,Wei Wei,Wang Zhenguang,Huang Yang
Luminescent copper nanoclusters (Cu NCs) are chosen to functionalize Ti 3 C 2 T x MXene flakes to form a new kind of nanohybrid. It was applied to the determination of glutathione (GSH) via photoluminescence (PL). The Cu NCs and MXene flakes are in close contact, and the blue PL of the Cu NCs (with excitation/emission peaks at 380/425 nm) is quenched. The addition of GSH triggers the separation of the nanohybrid. This results in the recovery of PL. GSH also promotes the PL of Cu NCs via host-guest interactions. Thus, target recognition, corresponding signal output and further magnification are accomplished in a single step. Under optimum conditions, the nanohybrid can detect GSH in the 5.0 to 100 μM concentration range and with a 3.0 μM detection limit. The assay is very specific and shows high selectivity towards metal ions, small biomolecules, amino acids, and thiol containing molecules.
Luminescent copper nanoclusters are used to functionalize Ti 3 C 2 T x MXene flakes, forming a nanohybrid, which is applied to detect glutathione. Target recognition, signal output and magnification are accomplished in a single step, resulting in high selectivity.
研究领域:
生物化学
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分析化学
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DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2024
·
IF 4.3
Zhifeng Cai,Yixuan Zhang,Manlin Zhao,Jinjia Bao,Ling Lv,Haoyang Li
Copper nanoclusters (FA@CuNCs) emitting blue fluorescence were successfully developed via a one-pot technique. In this method, the copper chloride, folic acid and hydrazine hydrate were applied as a precursor, protective agent and reducing agent, respectively. The absorption, fluorescence excitation and emission spectra of FA@CuNCs were carried out by using ultraviolet–visible and fluorescence spectrometry, respectively. The morphology, particle size, functional groups, oxidation states of elements of FA@CuNCs were discussed via using transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FT-IR) and X-ray photoelectron spectroscopy (XPS). The stability of FA@CuNCs was studied under various conditions, such as storage time at 25 ℃, ultraviolet radiation time, sodium chloride solutione and pH. The FA@CuNCs displayed blue fluorescence under the excitation wavelength of 361 nm, and the fluorescence quantum yield was 7.45 %. As a result of the inner filter effect, the alizarin red could significantly weaken the blue fluorescence of FA@CuNCs. Thus, the as-prepared FA@CuNCs could be utilized as fluorescence nanosensors for the trace determination of alizarin red. This platform suggested an excellent linear range for alizarin red varying from 0.5 to 200 μM with a fitting coefficient of 0.9955. The detection limit was calculated to be 0.064 μM in the light of the 3b/k (b and k refer to the standard deviation and slope of fitted curve, respectively). Furthermore, the as-developed FA@CuNCs could be used to detect the alizarin red in real samples and for the sensing of temperature.
研究领域:
化学
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材料科学
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分析化学
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PubMed
高影响因子
Analytical Methods
·
2019
·Vol. 11
·期 36
·4580-4585
·
IF 2.378
Xuan Wang,Caicheng Long,Zixin Jiang,Taiping Qing,Kaiwu Zhang,Peng Zhang,Bo Feng
Ascorbic acid (AA) plays an important role in many physiological and biochemical processes. Herein, a novel, rapid and label-free fluorescence strategy was proposed to detect ascorbic acid based on the in situ formation of copper nanoclusters (CuNCs). When reducing ascorbic acid was present, the Cu(II) ions in 3-(N-morpholino) propanesulfonic acid (MOPS) buffer were transformed into CuNCs and showed high fluorescence. The excitation and emission peaks of CuNCs were 340 nm and 580 nm, respectively. The fluorescence intensity was proportional to the concentration of ascorbic acid. Under optimized conditions, the linear range of ascorbic acid detection is 50 to 1000 μM, and the detection limit is 41.94 μM. In addition, this method is successfully applied to the determination of ascorbic acid in real samples (serum samples and vitamin C tablets) and has shown satisfactory results. Besides the simple operation and good detection capability, the signal response of this method to ascorbic acid is fast and can reach maximum saturation in a few minutes. There is no background interference due to the in situ formation of the signal unit (CuNCs). Therefore, the proposed strategy has a strong potential to detect ascorbic acid in medical diagnosis and food safety due to its excellent analytical sensitivity and high selectivity.
研究领域:
分析化学
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医学诊断
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食品安全
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DOI
高影响因子
JOURNAL OF LUMINESCENCE
·
2023
·
IF 3.6
Zhifeng Cai,Yi Zhang,Yiming Zhang,Yongjie Bai,Jiayue Huang,Zijing Guo,Huangjing Bao,Jiayi Zhang
Chrysin has carried out intensive interest in medicine areas due to its excellent influences for human health. Precise determination of chrysin is fairly necessary for pharmaceutical quality control and clinical study. Herein, water soluble fluorescent copper nanoclusters (Cu NCs) were developed through one-pot itinerary by utilizing polyvinylpyrrolidone as the stabilizer and ascorbic acid as the reducing agent. The as-obtained Cu NCs emitted bright blue fluorescence and suggested good optical features and outstanding photostability with the quantum yield of 9.27%. The inner filter effect (IFE) should be the main cause of the fluorescence quenching of Cu NCs. For this, a fluorescent system for chrysin determination was carried out by utilizing the Cu NCs as a nanoprobe. The fluorescence weakening efficiency of Cu NCs had a linear relationship to chrysin amounts in the range of 0.5–300 μM, and detection limit of 0.093 μM was obtained. This as-established system was effectively utilized to measure chrysin in real samples.
研究领域:
分析化学
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医学
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DOI
高影响因子
LUMINESCENCE
·
2024
·Vol. 39
·期 3
·e4702
·
IF 3.2
Yuyu Guo,Jiancheng Wang,Lili Zhang,Junkai Wang
Tannic acid (TA), as a stabilizing agent, was successfully utilized to establish blue-emitting copper nanoclusters (TA-Cu NCs) on the basis of a facile chemical reduction preparation method. Characterization results proved successful synthesis of TA-Cu NCs with uniform size and excellent stability. TA-Cu NCs exhibited a blue emission wavelength at 431 nm when excited at 364 nm. Interestingly, the as-prepared TA-Cu NCs were selectively quenched by furazolidone based on static quenching. In addition, this analysis platform for furazolidone detection had an excellent linear range from 0.5 to 120 μM with a detection limit of 0.074 μM ( S/N = 3). Furthermore, the accuracy of this sensing method was successfully confirmed by detecting furazolidone in bovine serum samples, indicating that TA-Cu NCs had bright application prospects.
研究领域:
材料科学
·
分析化学
·
食品科学
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DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2022
·
IF 5.518
Xian-song Wang,Shen Zhang
Herein, a platform for sensitive fluorescence sensing of morin was developed based on the copper nanoclusters , which was carried out by using a one-pot method. The copper nanoclusters (Trypsin@Cu NCs) were fabricated through CuCl 2 as a metal precursor, trypsin as a protective agent and hydrazine hydrate as a reducing agent. The Trypsin@Cu NCs exhibited blue fluorescence under the excitation wavelength of 378 nm. Using Trypsin@Cu NCs as the patterned nanosensors , the fluorescent “turn off” phenomenon was developed for the measurement of morin with the linear decline of emissive intensity at 459 nm. The fluorescence quenching reason was subjected to the static quenching and inner filter effect . Under the optimal conditions, an excellent linear response between ln(F 0 /F) of Trypsin@Cu NCs and morin amounts in the light of the range of 0.5–150 μM, and the limit of detection was 0.083 μM. Moreover, this proposed nanosensor could be applied for the sensing of temperature. This sensing method was displayed to be a practical determination system for the measurement of morin in real samples, which could open new horizons to develop novel detection models for morin.
研究领域:
分析化学
·
生物传感
·
材料科学
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DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Yidan Liang,Menglei Sun,Mao Wang,Haoting Hou,Shujie Pang,Zhe Zhang,Xudong Yang
In this work, a copper nanocluster (His-Cu NCs) with green fluorescence, which uses histidine (His) as the template agent, was developed. This nanomaterial was characterized using methods such as transmission electron microscopy (TEM). Based on this nanocluster, a fluorescence sensing platform was developed for the linear detection of Cr(VI) ions in solution. The surface of His-Cu NCs contains a large number of oxygen-containing and nitrogen-containing functional groups, which can react with Cr(VI), leading to charge transfer between ligands and metal ions, thereby quenching the fluorescence of His-Cu NCs. The sensor exhibits excellent sensitivity and selectivity for detecting Cr(VI), with a 0.31 μM detection limit for Cr(VI). Additionally, a fluorescent filter paper was created to detect Cr(VI). The fluorescence color of the fluorescent filter paper changed from green to blue with the increase in Cr(VI) concentration under the irradiation of a UV lamp at 365 nm. Furthermore, this His-Cu NCs’ fluorescence exhibits good stability, making it possible to employ the NCs as fluorescent ink for writing and drawing anti-counterfeiting icons on paper.
研究领域:
材料科学
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环境科学
·
分析化学
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DOI
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2021
·Vol. 413
·期 8
·2135-2146
·
IF 4.157
Mei He,Ma Yange,Wu Huimin,Wang Xuedong
Herein, we synthesized and characterized glutathione-capped copper nanoclusters (CuNCs) using a convenient one-pot chemical reduction approach based on glutathione as capping and reducing agents. The Ce(III) induced aggregation-induced emission of CuNCs to form a CuNCs-Ce 3+ fluoroprobe due to electrostatic and coordination interactions between Ce 3+ and CuNCs. In contrast to CuNCs, the fluorescent intensities (FLs) of CuNCs-Ce 3+ were enhanced by ~ 40-fold concomitant with 20-nm blue-shift of the maximum emission, and a 3.45-fold lengthening of the average fluorescent lifetime. The FLs of CuNCs-Ce 3+ were selectively quenched at 650 nm by hydrogen peroxide (H 2 O 2 ) via the redox reaction. Based on this phenomenon, the sensitive assay of H 2 O 2 was realized, and the linear range spanned over the range of 14–140 μM. Notably, the visualization of the fluorescence quenched effect of H 2 O 2 could be easily attained. Additionally, glucose could be specifically oxidized by glucose oxidase to produce H 2 O 2 , and thus the detection of glucose was achieved according to changes in the concentrations of H 2 O 2 . Under optimized conditions, the fluorescent assay of glucose based on the CuNCs-Ce 3+ system offered the linear range of 8–48 μM with detection limit of 2.4 μM. Meanwhile, high selectivity of the as-constructed fluorescent assay allows the sensitive detection of H 2 O 2 and glucose in real-world care products and human serum samples, showing a great application potential in their conventional monitoring.
研究领域:
分析化学
·
生物医学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）