BIOLOGICAL TRACE ELEMENT RESEARCH
·
2018
·Vol. 186
·期 2
·467-473
·
IF 2.361
Cao Changyu,Luo Junchong,Li Xiaowen,Zhang Mengdan,Zhang Haoji,Zhang Jipei,Wang Kai
The aim of this study was to evaluate the protective effects of SeY (selenium-rich yeast) against Al (aluminum)-induced inflammation and ionic imbalances. Male Kunming mice were treated with Al (10 mg/kg) and/or SeY (0.1 mg/kg) by oral gavage for 28 days. The degree of inflammation was assessed by mRNA expression of inflammatory biomarkers. Ionic disorders were assessed by determining the Na + , K + , and Ca 2+ content, as well as the alteration in ATP-modifying enzymes (ATPases), including Na + K + -ATPase, Ca 2+ -ATPase, Mg 2+ -ATPase, Ca 2+ Mg 2+ -ATPase, and the mRNA levels of ATPase’s subunits in kidney. It was observed here that SeY exhibited a significant protective effect on the kidney against the Al-induced upregulation of pro-inflammatory and downregulation of anti-inflammatory cytokines. Furthermore, a significant effect of Al on the Na + , K + , Ca 2+ , and Mg 2+ levels in kidney was observed, and Al was observed to decrease the activities of Na + K + -ATPase, Mg 2+ -ATPase, and Ca 2+ Mg 2+ -ATPase. The mRNA expression of the Na + K + -ATPase subunits and Ca 2+ -ATPase subunits was regulated significantly by Al. Notably, SeY modulated the Al-induced alterations of ion concentrations, ATPase activity, and mRNA expression of their subunits. These results suggest that SeY prevents renal toxicity caused by Al via regulation of inflammatory responses, ATPase activities, and transcription of their subunits.
研究领域:
毒理学
·
营养学
·
分子生物学
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产品关联
DOI
PubMed
高影响因子
BIOLOGICAL TRACE ELEMENT RESEARCH
·
2016
·Vol. 174
·期 1
·142-149
·
IF 1.798
Feng Tong,Liu Ping,Zhang Zhen,Hu Jinyu,Kong Zhengqiao
The study investigated the combined effect of 1,2-dimethyl-3-hydroxypyrid-4-one (DFP) and taurine on aluminum (Al) toxicity in cortex and blood of rats. The control group received 1 ml/kg/day saline solution for 8 weeks. Other animals were exposed to Al at a dose of 281.40 mg/kg/day orally for 4 weeks. Then, they were administered with 1 ml/kg/day saline solution, 400 mg/(kg·day) taurine, 13.82 mg/(kg·day) DFP, 27.44 mg/(kg·day) DFP, 400 mg/(kg·day) taurine +13.82 mg/(kg·day) DFP, and 400 mg/(kg·day) taurine +27.44 mg/(kg·day) DFP for 4 weeks. The changes in markers of oxidative stress, activities of antioxidant enzymes, and triphosphatase (ATPase) in the cortex and blood were determined. Administration of Al led to significant increase in the malondialdehyde (MDA) level and decrease in the activities of antioxidant enzymes, Na + K + -ATPase, Mg 2+ -ATPase, and Ca 2+ -ATPase in the cortex and blood, compared with the control group. DFP was observed to reverse alteration of these parameters except for Ca 2+ -ATPase activity. Treatment with taurine caused significant increase of GSH-Px activity and decrease of the MDA level in the cortex and serum and rise of Na + K + -ATPase in the blood. Effects of DFP combined with taurine were investigated and found to provide a more significant benefit than either drug alone. Combined intake of taurine and DFP could achieve an optimum effect of therapy for Al exposure.
研究领域:
药理学
·
毒理学
·
神经科学
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DOI
PubMed
高影响因子
TALANTA
·
2019
·
IF 4.916
Peng Luo,Youkun Zheng,Zhaojian Qin,Chunmei Li,Hui Jiang,Xuemei Wang
In this paper, a new strategy was presented for fluorescence labeling and imaging Al 3+ in live cells with excess aluminum ions using thiolated fluorescence gold nanoclusters (Au NCs). The glutathione (GSH)-capped Au NCs were prepared via a green, facile one-pot method in aqueous solution and displayed excellent stability, ultrasmall size, monodispersity, and larger Stokes shift, which exhibits a relatively weak fluorescence at 650 nm Al 3+ -induced fluorescence enhancement of the GSH-Au NCs can be observed due to Al 3+ -triggered aggregation-induced emission (AIE) effect, which allows the role of GSH-Au NCs as a fluorescence light-up probe for detection of Al 3+ . Moreover, it was demonstrated that the fluorescence probe for Al 3+ showed a wide detection range from 100 to 600 μM and good selectivity against other metal ions and common biomolecule. Furthermore, due to the advantages of excellent biocompatibility, low toxicity, red emission and high specificity, the proposed GSH-Au NCs fluorescence probes are suitable for the imaging of high concentrations of aluminum ions in cells, which can be applied to the diagnosis of cellular aluminum poisoning.
研究领域:
材料科学
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纳米技术
·
分析化学
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细胞生物学
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生物医学工程
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DOI
PubMed
高影响因子
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