TRANSITION METAL CHEMISTRY
·
2025
·
IF 1.7
Feng Qiying,Zhou Lin,Tian Shuang,Yang Jiawan,Liu Yunjun
Herein, we reported the synthesis and characterization of two new iridium(III) complexes [Ir(ppy) 2 (fpp)](PF 6 ) (Ir1a, ppy = 2-phenylpyridine, fpp = 2-(2,2-difluorobenzo[1,3]dioxol-5-yl-1H-imidazo[4,5-f][1,10]phenanthroline) and [Ir(bzq) 2 (fpp)](PF 6 ) (Ir1b, bzq = benzo[h]quinoline) through high resolution mass spectrometry (HRMS), 1 H NMR and 13 C NMR. The cytotoxicity in vitro of Ir1a and Ir1b on normal NIH3T3 cells and cancer SGC-7901, A549, SK-Hep1 cells was tested using MTT (3-(4,5-dimethylthiazole-2-yl)-diphenyltetrazolium bromide) method. Ir1a exhibits high cytotoxicity on SGC-7901 cells (IC 50 = 2.7 ± 0.7 µM), whereas Ir1b shows moderate cytotoxicity toward the selected cancer cells. The ROS content was investigated using a fluorescence probe of 2′,7′-dichlorodihydrofluorescein diacetate (DCHF-DA), the results show that Ir1a and Ir1b elevate ROS content. The co-localization and the change of mitochondrial membrane potential were explored. Apoptotic studies using Annex V/PI double staining method demonstrate that Ir1a and Ir1b can efficiently cause apoptosis. Ir1a and Ir1b inhibit the cell proliferation at the G2/M period. Additionally, lipid peroxidation and downregulation of ferritin protein suggest that Ir1a and Ir1b can trigger ferroptosis.
研究领域:
药理学
·
肿瘤学
·
生物化学
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产品关联
DOI
高影响因子
DYES AND PIGMENTS
·
2023
·
IF 4.5
Yi-Chuan Huang,Xi Chu,Wen-Hao Li,Shi-Sheng Zhao,Jia-Xin Zhang,Zhen-Qiang Qin,Hong-Yan Li,Wei Xue
Excessive thiourea(TU) was demonstrated to be harmful to both environmental system and human body, so it is essential to create selective and sensitive sensors to detect TU. Herein, Four iridium(III) complexes, [(fppy) 2 Ir(PD)]PF 6 ( Ir-fppy , fppy = 2-(4-fluorophenyl)pyridine), [(fpiq) 2 Ir(PD)]PF 6 ( Ir-fpiq , fpiq = 1-(4-fluorophenyl)isoquinoline), [(dfppy) 2 Ir(PD)]PF 6 ( Ir-dfppy , dfppy = 2-(3,5-difluorophenyl)pyridine) and [(dfpiq) 2 Ir(PD)]PF 6 ( Ir-dfpiq , dfpiq = 1-(3,5-difluorophenyl)isoquinoline) bearing 1,10-phenanthroline-5,6-dione (PD) as ancillary ligands, were designed and synthesized, and employed as efficient chemosensors to identify thiourea . These iridium(III) complexes exhibited emission peaks between 541 nm and 583 nm with PLQY ranging from 0.12 to 0.26 and lifetime in the range of 2.65–2.74 μs. All the four iridium(III) complexes showed significant turn-on response to TU with low limit of detect on the order of 10 −8 M. Furthermore, these iridium(III) complexes-based chemosensors exhibited excellent recoveries toward TU in various juice samples including orange, kiwi fruit and lemon juice. Four complexes-composite test strips showed marker color change after adding TU.
研究领域:
化学传感器
·
分析化学
·
环境科学
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产品关联
DOI
高影响因子
JOURNAL OF MOLECULAR STRUCTURE
·
2025
·
IF 4.7
Jinshan Lin,Shengjie Wang,Pengchong Jiao,Mei-Jin Li
A highly charged water-soluble iridium(III) (Ir(III)) complex [(ppy) 2 Ir(bpy-N)] 3+ was designed, synthesized and characterized, and the crystal structure of it was determined by X-ray crystallography. The photophysical properties of complex in absence or presence of sodium heparin were studied by UV–vis absorption and photoluminescent spectroscopies. After addition of sodium heparin, the luminescent intensity of complex at ca. 656 nm was increased about 4.3-fold, without any observable color change in the solution. The new Ir(III) complex was also assembled with gold nanoparticles (AuNPs) for the dual-mode detection of sodium heparin, combined with colorimetric and luminescent methods. In this system, the luminescence of complex was quenched by AuNPs firstly with the red color of gold nano colloids transition into blue due to the aggregation. The color of the assembled system changed from blue to purple to red accompanying with the luminescent intensity of system restoring after increasing the concentrations of added sodium heparin. The sodium heparin was sensitively detected with LOD of 0.09 μg/mL. This work demonstrates a novel bifunctional sensing platform for sodium heparin, leveraging the unique photophysical properties of the Ir(III) complex and the plasmonic behavior of AuNPs, offering significant potential for sensitive and visual detection applications.
研究领域:
分析化学
·
纳米医学
·
药物分析
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产品关联
DOI
高影响因子
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
·
2024
·
IF 4.1
Yuxiang Jiao,Yifei Li,Huaijun Tang,Shanglan Xian,Yonghui He,Long Wang,Jinghao Zhang
Herein, a cationic iridium(III) complex [(ppy) 2 Ir(IPP)]PF 6 (ppy: 2-phenylpyridine; IPP: 2-(1 H -imidazo[4,5- f ][1,10]phenanthrolin-2-yl)phenol) was synthesized. Under the excitation of 328 nm (λ ex = 328 nm) UV light, this complex emitted bright yellow orange light (500 ∼ 700 nm, λ em,max = 582 nm), the Stokes shift was up to 254 nm, mainly due to excited-state intramolecular proton transfer (ESIPT) occurring in its ancillary ligand IPP. [(ppy) 2 Ir(IPP)]PF 6 was very sensitive to Ni 2+ , which was caused by the ESIPT being hindered by the coordination of [(ppy) 2 Ir(IPP)]PF 6 with Ni 2+ . Its bright yellow orange light disappeared within 1 min when Ni 2+ (1.0 equiv.) was added in its solution (in DMSO/H 2 O, v/v = 999/1), therefore, it can be used for “turn-off” chemiluminescent detection of Ni 2+ . The recognition of [(ppy) 2 Ir(IPP)]PF 6 to Ni 2+ was not interfered by many common metal ions (such as Na + , K + , Li + , Mg 2+ , Al 3+ , Ca 2+ , Fe 3+ , Ag + , Zn 2+ , Hg 2+ , Co 2+ , Pb 2+ , Mn 2+ , Ba 2+ or Cr 3+ ), however, Cu 2+ was an interfering metal ion, but its interference can be completely masked by thiourea. The detection limit was as low as 3.31 × 10 −7 mol∙L -1 (0.331 μM). The Job’s plot analysis revealed that the stoichiometry of [(ppy) 2 Ir(IPP)]PF 6 with Ni 2+ was 1:1, its detecting mechanism based on ESIPT effect was demonstrated by 1 H NMR spectra and density functional theory (DFT) calculation.
研究领域:
化学
·
分析化学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
ANALYST
·
2012
·Vol. 137
·期 22
·5398-5402
·
IF 4.23
Qun-bo Mei,Yuan-hui Guo,Bi-hai Tong,Jie-Na Weng,Bin Zhang,Wei Huang
A new phosphorescent chemosensor for Hg2+ and acetonitrile (MeCN) based on iridium(III) complex Ir(dpp)2(dtc) (Ir1, dppH = 4,6-diphenylpyrimidine, dtcH = diethyl dithiocarbamic acid) was realized. Upon addition of a tetrahydrofuran (THF) solution of Hg2+, the dichloromethane (DCM) solution of Ir1 gave a visual color change and significant fluorescent quenching. When MeCN was added, a new fluorescent emission emerged, which constituted a selective MeCN phosphorescent chemosensor. Complex Ir1 has been developed as an AND logic gate with Hg2+ and MeCN as inputs.
研究领域:
分析化学
·
配位化学
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DOI
PubMed
高影响因子
PLoS One
·
2015
·Vol. 10
·期 3
·e0121293
·
IF 3.234
Yun Zeng,Yang Liu,Jin Shang,Jingwen Ma,Rong Wang,Lei Deng,Youmin Guo,Fan Zhong,Mingfeng Bai,Shaojuan Zhang,Daocheng Wu
Objectives
To utilize phosphorescence to monitor hypoxic microenvironment in solid-tumors and investigate cancer chemotherapeutic effects in vivo .
Methods
A hypoxia-sensitive probe named BTP was used to monitor hypoxic microenvironment in solid-tumors. The low-dose metronomic treatment with cisplatin was used in anti-angiogenetic chemotherapeutic programs. The phosphorescence properties of BTP were detected by a spectrofluorometer. BTP cytotoxicity utilized cell necrosis and apoptosis, which were evaluated by trypan blue dye exclusion and Hoechst33342 plus propidium iodide assays. Tumor-bearing mouse models of colon adenocarcinoma were used for tumor imaging in vivo . Monitoring of the hypoxic microenvironment in tumors was performed with a Maestro 2 fluorescence imaging system. Tumor tissues in each group were harvested regularly and treated with pathological hematoxylin and eosin and immunohistochemical staining to confirm imaging results.
Results
BTP did not feature obvious cytotoxicity for cells, and tumor growth in low-dose metronomic cisplatin treated mice was significantly inhibited by chemotherapy. Hypoxic levels significantly increased due to cisplatin, as proven by the expression level of related proteins. Phosphorescence intensity in the tumors of mice in the cisplatin group was stronger and showed higher contrast than that in tumors of saline treated mice.
We develop a useful phosphorescence method to evaluate the chemotherapeutic effects of cisplatin. The proposed method shows potential as a phosphorescence imaging approach for evaluating chemotherapeutic effects in vivo , especially anti-angiogenesis.
研究领域:
肿瘤学
·
医学影像学
·
药理学
✓ 已验证
DOI
PubMed
高影响因子
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
·
2024
·
IF 6
Huiyan Hu,Fan Zhang,Zhujun Sheng,Shuang Tian,Gechang Li,Shuanghui Tang,Yajie Niu,Jiawan Yang,Yunjun Liu
This paper introduces a new ligand, 4,6-dichloro-5-(1 H -imidazo [4,5-f]phenanthroline-2-yl)pyrimidin-2-amine (DPPA), and its corresponding new iridium(III) complexes: [Ir(ppy) 2 (DPPA)](PF 6 ) (2a) (where ppy represents deprotonated 2-phenylpyridine), [Ir(bzq) 2 (DPPA)](PF 6 ) (2b) (with bzq indicating deprotonated benzo[h]quinoline), and [Ir(piq) 2 (DPPA)](PF 6 ) (2c) (piq denoting deprotonated 1-phenylisoquinoline). The cytotoxic effects of both DPPA and 2a, 2b, and 2c were evaluated against human lung carcinoma A549, melanoma B16 , colorectal cancer HCT116, human hepatocellular carcinoma HepG2 cancer cell lines, as well as the non-cancerous LO2 cell line using the 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method. While DPPA exhibited moderate anticancer activity toward A549, B16, HCT116 and HepG2 cells, complexes 2a, 2b, and 2c displayed remarkable efficacy against A549, B16, and HCT116 cells. The cell colonies and wound healing were investigated. Moreover, various aspects of the anticancer mechanisms were explored. The cell cycle analyses revealed that the complexes block cell proliferation of A549 cells during the S phase. Complex 2c induce an early apoptosis, while 2a and 2b cause a late apoptosis. The interaction of 2a, 2b and 2c with endoplasmic reticulum and mitochondria was identified, leading to elevated ROS and Ca 2+ amounts. This resulted in a reduced mitochondrial membrane potential , mitochondrial permeability transition pore opening, and an increase of cytochrome c . Also, ferroptosis was investigated through measurements of intracellular glutathione (GSH), malondialdehyde (MDA), and recombinant glutathione peroxidase (GPX4) protein expression . The pyroptosis was explored via cell morphology, release of lactate dehydrogenase (LDH) and expression of pyroptosis-related proteins. RNA sequencing was applied to examine the signaling pathways . Western blot analyses illuminated that the complexes regulate the expression of Bcl-2 family proteins. Additionally, an in vivo antitumor study demonstrated that complex 2c exhibited a remarkable inhibitory rate of 58.58% in restraining tumor growth. In summary, the findings collectively suggest that the iridium(III) complexes induce cell death via ferroptosis , apoptosis by a ROS-mediated mitochondrial dysfunction pathway and GSDMD-mediated pyroptosis .
研究领域:
肿瘤学
·
药理学
·
细胞生物学
✓ 已验证
DOI
高影响因子
INORGANIC CHEMISTRY COMMUNICATIONS
·
2025
·
IF 5.4
Yi-Heng Wang,Yang Liu,Jin Tong,Shu-Yan Yu
The cyclometalated Ir(III) complex, Ir(ppy) 2 (phen-dipzH 2 ) ( Ir-1 ), was synthesized using the ligand 3,8-di(1H-pyrazol-4-yl)-1,10-phenanthroline (phen-dipzH 2 ) and the auxiliary ligand 2-phenylpyridine (ppy). Crystal structure analysis reveals that each {Ir(ppy) 2 } + unit is coordinated by a neutral phen-dipzH 2 ligand in Ir-1 . The packing arrangement of Ir-1 displays aromatic stacking interactions and [C–H…N] hydrogen bonding interactions between the [Ir(ppy) 2 (phen-dipzH 2 )] + cations. In solution, Ir-1 exhibits emission switching induced by acid/base changes, with the emission wavelength shifting from 562 nm (yellow color) in the neutral state to 618 nm (pink color) under acidic conditions and to 528 nm (gray color) under basic conditions. These shifts are attributed to the protonation and deprotonation of the pyrazole units, which is confirmed by 1 H NMR spectra. Additionally, Ir-1 demonstrates high selectivity and sensitivity toward Cu 2+ ions, with a detection limit as low as 9.47 μM compared to other metal ions. The acid/base-responsive phosphorescence of Ir-1 has also been successfully utilized in the development of paper strip-based devices for urea detection.
研究领域:
化学
·
材料科学
·
分析化学
✓ 已验证
DOI
高影响因子
Advanced Science
·
2021
·Vol. 8
·期 8
·2004044
·
IF 16.806
Peng Xiao,Chunyan Liu,Tiancong Ma,Xiuhong Lu,Lihong Jing,Yi Hou,Peisen Zhang,Gang Huang,Mingyuan Gao
Hypoxia is considered as a key microenvironmental feature of solid tumors. Luminescent transition metal complexes particularly those based on iridium and ruthenium have shown remarkable potentials for constructing sensitive oxygen-sensing probes due to their unique oxygen quenching pathway. However, the low aqueous solubility of these complexes largely retards their sensing applications in biological media. Moreover, it remains difficult so far to use the existing complexes typically possessing only one luminescent domain to quantitatively detect the intratumoral hypoxia degree. Herein, an Ir(III) complex showing red emissions is designed and synthesized, and innovatively encapsulated within a hydrophobic pocket of Cyanine7-modified cyclodextrin. The Ir(III) complex enables the oxygen detection, while the cyclodextrin is used not only for improving the water solubility and suppressing the luminescence quenching effect of the surrounding aqueous media, but also for carrying Cyanine7 to establish a ratiometric oxygen fluorescence probe. 2D nuclear magnetic resonance is carried out to confirm the host–guest structure. The oxygen-responsive ability of the resulting ratiometric probe is evaluated through in vitro cell and multicellular experiments. Further animal studies about tumor oxygen level mapping demonstrate that the probe can be successfully used for quantitatively visualizing tumor hypoxia in vivo.
研究领域:
生物医学工程
·
肿瘤学
·
分析化学
✓ 已验证
DOI
高影响因子
ANALYST
·
2010
·Vol. 136
·期 1
·205-210
·
IF 3.272
Mei-Jin Li,Pengchong Jiao,Min Lin,Weiwen He,Guo-Nan Chen,Xi Chen
A new water-soluble iridium(III) diimine complex with appended sugar was synthesized and characterized. The electrochemiluminescent behavior of the new complex in aqueous buffer was first studied and the ECL signal was found to be much higher than that of [Ru(bpy)3]2+ at a Pt working electrode. Tri-n-propylamine (TPA) and antibiotics were determined by the ECL of the iridium(III) complex in aqueous buffer at the Pt electrode and the method was found to show good sensitivity and reproducibility. The new iridium(III) complex was found to display good solubility in aqueous solution and a strong ECL signal at the Pt electrode, which might open up the possibility of its application in analysis.
研究领域:
分析化学
·
电化学
·
材料科学
✓ 已验证
DOI
高影响因子
Journal of Materials Chemistry B
·
2019
·Vol. 7
·期 47
·7612-7618
·
IF 5.047
Yuanyan Li,Yongquan Wu,Luyan Chen,Hong Zeng,Xiaoyong Chen,Weican Lun,Xiaolin Fan,Wai-Yeung Wong
Peroxynitrite (ONOO−), one of the reactive oxygen/nitrogen species (ROS/RNS) found in vivo, plays crucial roles in many physiological and pathological processes. The ability to selectively and sensitively determine ONOO−in vivo is important for the understanding of its biological roles. Thus, by utilizing the excellent chemical stability and photostability, high luminescence efficiency, and long luminescence lifetime of iridium complexes, we developed a novel near-infrared (NIR) phosphorescent iridium(III) complex (FNO2) probe to detect ONOO− within seconds. The probe FNO2 showed better selectivity towards ONOO− over other interfering biomolecules, including O2− and ClO−. Moreover, it possessed a long luminescence lifetime, which enabled successful elimination of the interference from background fluorescence in vitro (simulated by Rhodamine B) in time-resolved emission spectra. Finally, in addition to its low cytotoxicity, the probe FNO2 showed emission wavelength in the NIR region and was able to specifically sense ONOO− induced in living cells and inflamed mouse models.
研究领域:
生物化学
·
医学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2022
·Vol. 414
·期 13
·3755-3763
·
IF 4.478
Wu Shujie,Wang Songen,Li Zongbing,Wu Chun,Ma Dik-Lung,Miao Xiangmin
In this work, we synthesized an iridium(III) complex and studied its selective ability to interact with a specific G-quadruplex DNA sequence (GTGGGTAGGGCGGGTTGG). Results showed that the iridium(III) complex exhibits high selectivity for the G-quadruplex DNA and could be used as an efficient electrochemiluminescence (ECL) probe in a switch-on assay format for the detection of double-stranded DNA (dsDNA). To construct the assay, a hairpin-structured capture probe (CP) which was modified by thiol at its 3′ end and contained the G-quadruplex sequence at its 5′ end was firstly immobilized on a gold electrode. Upon the specific recognition of the dsDNA sequence with the corresponding CP, the hairpin structure of the CP was opened to free G-quadruplex sequence, forming the G-quadruplex structure with the assistance of K + . Then, the iridium(III) complex was able to specifically interact with the G-quadruplex to produce an obvious ECL signal that was proportional to the dsDNA concentration. Notably, this iridium(III) complex/G-quadruplex-based strategy was universal and was not limited to the analysis of DNA using specific sequences, thus opening a new avenue for the application of the G-quadruplex-selective iridium(III) complex in the field of ECL.
研究领域:
生物化学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
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