INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
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2015
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IF 3.082
Dongyang Xu,Qing Nie,Wenyan Wang,Lei Shi,He Yan
In this study, we characterized a bcrABC cassette and its genetic environment harbored by a plasmid in Listeria monocytogenes ( L . monocytogenes ) 11GZL18, a strain isolated from raw meat in 2011. The bcrABC cassette and its genetic environment were characterized, with a total of 33,727 nt nucleotide sequence obtained. The nucleotide sequences of the bcrABC cassette in strain 11GZL18 exhibited 100% identity to that on plasmid pLM80, which is harbored by L . monocytogenes strain H7550and H7858, and the neighboring 21,678 nt nucleotide sequence of bcrABC cassette showed 99% identity with plasmid pLM80. The plasmid curing experiment demonstrated the role of the plasmid in conferring benzalkonium chloride (BC) and cadmium (Cd) tolerance in this strain. The bcrABC cassette and cadAC genes from the L . monocytogenes 11GZL18 were harbored by plasmid, functional and transmissible, and led to the acquired tolerance in Gram-negative Escherichia coli ( E . coli ) DH5α by chemical and natural transformation. Besides, the efflux pump activity that is conferring tolerance to BC and Cd was observed in strain 11GZL18, while not in a plasmid-cured strain 11GZL18-C, confirming that efflux pumps play a role in plasmid-mediated tolerance to BC and Cd in L . monocytogenes 11GZL18. In this study we characterized the genetic organization of a novel BC and Cd tolerance determinants-harboring plasmid in a L . monocytogenes strain isolated from raw meat of animal origin, and demonstrated the potential horizontal transferability of this bcrABC cassette-harboring plasmid to E . coli . The findings will further improve our understanding of the adaptations of this organism to disinfectants such as BC and may contribute to elucidating possible dissemination of BC tolerance in foodborne L . monocytogenes .
研究领域:
微生物学
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食品安全
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分子生物学
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产品关联
DOI
PubMed
高影响因子
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
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2025
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IF 3.3
Wang Yanyan,Peng Yu,Zhu Huan,Zhou Wenjun,Bi Yonghong,Chen Lin,Liu Tianzhong
High-ammonium wastewater can be simultaneously remediated and valorized through phototrophic cultivation of stress-resilient microalgae. This study evaluated the growth performance of 16 microalgae strains (specific growth rate μ = 0.108–0.217 day −1 ) from different terrestrial habitats, recognizing that there is a high diversity of terrestrial microalgae, which could harbor species with unique metabolites and properties, among which Tribonema arborum HJX, Cephaleuros virescens LST0102, and Cephaleuros virescens 42.85 exhibited high biomass productivity, reaching up to 190.6 mg L −1 day −1 . Tribonema arborum HJX demonstrated the highest production of lipids and carotenoid, with a total of 46.9 and 2.51 mg L −1 day −1 , respectively. The tolerance of these strains to ammonium and low pH, along with their performance in a 10-L panel PBR, was then assessed. The results revealed that filamentous algae isolated from terrestrial habitats have significant potential for the co-production of lipids and carotenoids, as well as robust growth capabilities under high-ammonium and low-pH environmental stress. This study highlights the potential of terrestrial filamentous algae in co-producing high-value chemicals with high-ammonium wastewater.
研究领域:
微生物学
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生物技术
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环境科学
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藻类学
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生物工程
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DOI
PubMed
高影响因子
Nature Plants
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2023
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IF 18
Xie Yuanming,Lv Yuanda,Jia Letian,Zheng Lulu,Li Yonghui,Zhu Ming,Tian Mengjun,Wang Ming,Qi Weicong,Luo Long,De Gernier Hugues,Pélissier Pierre-Mathieu,Motte Hans,Lin Shaoyan,Luo Le,Xu Guohua,Beeckman Tom,Xuan Wei
Ammonium toxicity affecting plant metabolism and development is a worldwide problem impeding crop production. Remarkably, rice ( Oryza sativa L.) favours ammonium as its major nitrogen source in paddy fields. We set up a forward-genetic screen to decipher the molecular mechanisms conferring rice ammonium tolerance and identified rohan showing root hypersensitivity to ammonium due to a missense mutation in an argininosuccinate lyase (ASL)-encoding gene. ASL localizes to plastids and its expression is induced by ammonium. ASL alleviates ammonium-inhibited root elongation by converting the excessive glutamine to arginine. Consequently, arginine leads to auxin accumulation in the root meristem, thereby stimulating root elongation under high ammonium. Furthermore, we identified natural variation in the ASL allele between japonica and indica subspecies explaining their different root sensitivity towards ammonium. Finally, we show that ASL expression positively correlates with root ammonium tolerance and that nitrogen use efficiency and yield can be improved through a gain-of-function approach.
研究领域:
植物科学
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分子生物学
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农业
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）