Qingling Nie,Peipei Xu,Jiajia Liu,Yanhanzhuo Wang,Qian Huang,Ruiyun You,Yunzhen Liu,Lizhi Li,Yudong Lu
To improve the sensitivity, accuracy and specificity of the assay, a two-dimensional silver substrate with EF=5.85×10 8 was first synthesized as a SERS substrate, on the surface of which DSP molecules were modified to form a DSP-antibody coupling through the activation of two N-hydroxy succinimide (NHS) esters to capture TNF-α. Subsequently, aptamerized silver-coated gold nanospheres (Au@TFMBA@Ag) were synthesized as Surface-Enhanced Raman Scattering (SERS) recognition probes. These probes were employed to create a sandwich structure for the quantitative detection of Tumor Necrosis Factor-alpha (TNF-α), utilizing the SERS signal intensity at 1374 cm −1 . Quantitative detection of TNF-α was successfully accomplished within the concentration range of 10 −4 to 10 −10 mg·mL −1 . Clinical serum samples were collected and subjected to testing. Significance analysis, conducted through the T-test (p < 0.0001), unequivocally showed the method's ability to differentiate between sera from normal individuals and those diagnosed with colon cancer.
The interfacial behaviour of ortho -positioned diaryl disulfide on activated silver substrates was characterized with surface-enhanced Raman scattering and electroreduction. After the fracture of the disulfide bond, the diaryl disulfides interacted with Ag to form self-assembled monolayers. The peak intensity of benzene ring deformation and pH-dependence presented two types of linear relationships. The probe molecule at pH 11.5 was used to estimate the Raman enhancement factor of silver substrates. Also, the alkaline media were favorable for the active intermediates of ortho -positioned diaryl disulfide to interact on the liquid-solid silver interfaces. Linear regression fitting between the reciprocals of surface coverage and those of solution concentration from reductive desorption was observed, and meaningful progress of spectroelectrochemical mechanisms was made for surface chemistry.
Graphical Silver linings : A large-area, self-assembling, octahedral aggregate composed of poly(methyl methacrylate) nanospheres (PMMA-NS) was constructed by a one-step electrodeposition approach using an aqueous solution of AgNO 3 and PMMA-NS as sacrificial electrolytes. The surface chain geometry of the product was revealed by Raman spectroscopy, and its excellent adsorptive properties were studied (see figures). A large-area, self-assembling, octahedral aggregate composed of poly(methyl methacrylate) nanospheres (PMMA-NS) was constructed by using a one-step electrodeposition approach from an aqueous solution containing AgNO 3 and PMMA-NS as sacrificial electrolytes. The surface chain geometry of the product was revealed by Raman spectroscopy. The results revealed that the orientation of chains of the PMMA-NS octahedron is different from that of the nonaggregated PMMA-NS because of the interfacial interactions between the polymer chain and Ag metal. This can be explained by the crystal surface free energy of the octahedral Ag substrate, which gives rise to diverse conformations of the PMMA molecular chain at different surface regions of the silver substrate. In addition, the adsorptive properties of PMMA-NS octahedra are such that the adsorbent–adsorbate system is cost-effective, efficient and fast for the removal of dyes from contaminated wastewater.
危险品化学品经营许可证（带存储）