By self-assembly of MnCl 2 and arginine under alkaline conditions, ultra-small MnArg nanoparticles were successfully constructed as oxidase (OXD) mimics for intelligent detection of the Ginkgo toxin 4′- O -methylpyridoxal (MPN). The obtained MnArg nanozymes possessed excellent OXD-like activity and thermal stability. Based on the inhibitory effect of MPN for the catalytic activity of MnArg, this system was utilized for the colorimetric sensing of MPN with a low limit of detection (LOD) of 2.16 µg mL −1 . The detection system exhibited good selectivity against other potential interferents. FTIR data showed that the presence of MPN binds with MnArg and shields the active sites, thereby interfering with the oxidase-like activity. Combined with a smartphone and the ColorMax software, this nanozyme-based intelligent detection platform could effectively detect MPN with a LOD of 2.1 µg mL −1 . Our MnArg nanozyme–based system was applied to detect real ginkgo nut samples with recoveries of 92.4–108.7%, and the relative standard deviations were less than 0.7%. This work may promote the development of novel nanozymes and expand their applications in the field of food safety detection. Graphical Abstract
Rong Wu,Minmin Zou,Jiarui Cao,Manman Shi,Guobin Jiang,Fuliang Cao,Erzheng Su
Ginkgo seeds are rich in nutrients and bioactive compounds, but they cause poisoning after excessive consumption, thus limiting their application. In this work, enzymatic degradation technology was employed to remove ginkgotoxins presented in ginkgo seeds, including 4’-O-methylpyridoxine (MPN) and MPN-5’-glucoside (MPNG). Through the screening of four oxidases and ten reaction mediators, the laccase-syringaldehyde system was successfully established. After optimizing the reaction conditions using response surface methodology (RSM), ginkgotoxins in both raw and boiled ginkgo seed powder was completely degraded at a temperature of 40 °C and a solvent to solid ratio (60 mL/g) of the ginkgo seed powder suspension within 3 h. Furthermore, the main nutritional components of detoxified ginkgo seed powder did not exhibit significant changes, but there was inevitably a decrease in the content of bioactive compounds. Finally, an acute oral toxicity assessment demonstrated that the detoxified ginkgo seed powder was virtually non-toxic. Therefore, enzymatic degradation using the laccase-syringaldehyde system has the potential to be a simple and effective approach for removing ginkgotoxins from ginkgo seed powder, thereby meeting the safety requirements for ginkgo products.
危险品化学品经营许可证（带存储）