INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
·
IF 8.5
Yuanhong Cai,Peipei Xu,Runbing Huang,Jiayi Huang,Junhua Zhan,Junpeng Su,Ruiyun You,Yudong Lu
This study presents the development of a novel polysaccharide biomolecule composite microsphere system based on sodium alginate (SA) and tremella fuciformis polysaccharide (TFP) for the encapsulation and controlled release of camellia oil (CO). The system leverages the synergistic properties of these two bioactive biopolymers to enhance the protection and bioavailability of CO during simulated digestion. The microspheres, characterized by their spherical structure and uniform particle size distribution, were comprehensively evaluated for stability, elemental composition, and thermal stability using techniques such as scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), Fourier transform infrared spectroscopy (FT-IR), and thermogravimetric analysis (TGA). The results confirmed a significant improvement in the elemental composition and surface properties of the microspheres due to the incorporation of CO, which enhanced their stability. In simulated digestion experiments, the microspheres demonstrated excellent gastric acid tolerance and intestinal release, achieving a high encapsulation efficiency of 99.59 % and an oil loading capacity of 80.67 %, effectively protecting and targeting the release of CO. Moreover, the microspheres exhibited significant antibacterial activity against Staphylococcus aureus ( S. aureus ) and Escherichia coli ( E. coli ). Overall, the SA/TFP/CO microspheres represent an ideal delivery system for CO, demonstrating considerable potential in the fields of food science and biomedicine.
研究领域:
食品科学
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生物医学
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产品关联
DOI
PubMed
高影响因子
PACKAGING TECHNOLOGY AND SCIENCE
·
2024
·
IF 2.8
Lin Lin,Xu Liu,Ce Shi,Xiaochen Chen,Tariq Aziz,Fahad Al-Asmari,Amal A. Mohamed,Haiying Cui
The objective of this research was to develop and analyse a chitosan- Tremella fuciformis polysaccharide (CS-TFP) edible film using chitosan (CS) and Tremella fuciformis polysaccharide (TFP) as the primary raw materials. The findings revealed significant improvements in the thickness, solubility, opacity, mechanical properties and barrier properties (water vapour and oxygen permeability) of the chitosan film upon the addition of TFP, but water content and hydrophobicity were reduced. Moreover, TFP addition markedly enhanced the antibacterial and antioxidant activities of the chitosan films. Structural analysis of the CS films and CS-TFP edible films indicated that the formation of hydrogen bonds and high compatibility between CS and TFP. Specifically, TFP increased crystallinity, reduced roughness and improved the compactness of the edible film. This increase in compactness was directly linked to enhanced physical properties, whereas increased crystallinity contributed to better thermal stability. Furthermore, the application of CS-TFP-3 edible films in chicken preservation proved that they could significantly delay the deterioration of chicken. Findings in this study suggested that CS-TFP edible film as a bioactive packaging material showed great potential to preserve chicken meat and extend the shelf life.
研究领域:
食品科学
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材料科学
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DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2023
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IF 8.2
Xiaokun Han,Tianyun Lu,Zuocai Zhang,He Wang,Shaorong Lu
The application of conductive hydrogels in flexible wearable devices has garnered significant attention. In this study, a self-healing, anti-freezing, and fire-resistant hydrogel strain sensor is successfully synthesized by incorporating sustainable natural biological materials, viz. Tremella polysaccharide and silk fiber, into a polyvinyl alcohol matrix with borax cross-linking. The resulting hydrogel exhibits excellent transparency, thermoplasticity, and remarkable mechanical properties, including a notable elongation (1107.3 %) and high self-healing rate (91.11 %) within 5 min, attributed to the dynamic cross-linking effect of hydrogen bonds and borax. A strain sensor based on the prepared hydrogel sensor can be used to accurately monitor diverse human movements, while maintaining exceptional sensing stability and durability under repeated strain cycles. Additionally, a hydrogel touch component is designed that can successfully interact with intelligent electronic devices, encompassing functions like clicking, writing, and drawing. These inherent advantages make the prepared hydrogel a promising candidate for applications in human health monitoring and intelligent electronic device interaction.
研究领域:
材料科学
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
Foods
·
2023
·Vol. 12
·期 8
·1609
·
IF 5.2
Xiaofang Zhao,Zhiyu Wang,Yingxu Liu,Zhaolian Han,Tingting Liu,Zhiqiang Cheng
In this paper, the effects of different proteins (soybean protein isolate, wheat protein hydrolysate, tremella protein) on the activity of tremella polysaccharide under different conditions were studied. The optimal protein–polysaccharide complex was determined by grafting degree and activity screening, and the microstructure and rheological properties were studied. The results showed that when the ratio of soybean protein isolate to tremella polysaccharide was 2:1 and the solution pH was 7, the optimal complex was obtained by heating at 90 °C for 4 h, and its grafting degree and antioxidant activity were the best. Studies have shown that tremella polysaccharide and soybean protein isolate complex (TFP-SPI) solution is pseudoplastic fluids. At the same time, tremella polysaccharide (TFP) and TFP-SPI were used for electrospinning to observe its spinnability. When the ratio of PVA/TFP-SPI/PL was 8:1:1, nanofibers with uniform diameter and good morphology were obtained. This paper provides a theoretical basis for the comprehensive utilization of tremella polysaccharide and its electrospun fiber can be used as active film for food packaging.Keywords:tremella polysaccharide;protein complex;antioxidant activity;rheological property;electrostatic spinning
研究领域:
食品科学
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生物化学
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
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2024
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IF 13.3
Zhijia Liao,Ninghao Chu,Zhenyu Yuan,Yanbai Shen,Fanli Meng
This study successfully synthesized “silver ear-like” Co(OH)F using 15 nm SiO 2 spheres as seeds. Gas sensitivity of Co(OH)F with and without SiO 2 spheres to toluene was compared at different annealing temperatures. The results demonstrate that the Co(OH)F-Si-350 sensor exhibits optimal gas sensitivity to toluene, with a response of 35 (R g /R a ) to 100 ppm of toluene at 220℃, with response and recovery times of 50 s and 80 s, respectively. Notably, the sensor detected toluene concentrations as low as 50 ppb, indicating good sensitivity to low concentrations. These findings suggest that the structural transformation resulting from the introduction of SiO 2 nanospheres, forming ultra-thin nanosheet clusters (∼12 nm), provides abundant active sites, effectively promoting substance diffusion and adsorption/desorption processes. Additionally, the incorporation of fluorine enhances the Lewis acidity of the material, facilitating the formation of stable adsorption states for gas molecules on the surface. These results reveal the significant impact of the structural transformation caused by the introduction of SiO 2 nanospheres on gas sensitivity, providing a strong theoretical foundation for further optimizing gas sensor design.
研究领域:
材料科学
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传感器技术
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化学工程
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产品关联
DOI
高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
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2024
·
IF 3.3
Haizhao Song,Jing Lu,Qiang Chu,Rou Deng,Xinchun Shen
BACKGROUND Because of their diverse biological activities, polysaccharides derived from Tremella fuciformis have received growing attention. This study aimed to investigate the structural characterization of a purified polysaccharide (designated as PTP-3a) derived from T. fuciformis and explore its interaction with gut microbiota in vitro . RESULTS The findings revealed that PTP-3a had a molecular weight of 1.22 × 10 3 kDa and consisted of fucose, glucose, xylose, mannose and glucuronic acid in a molar ratio of 0.271:0.016:0.275:0.400:0.038. The primary linkage types identified in PTP-3a were 1,3-linked-manp, 1,4-linked-xylp and 1,2,3-linked-fucp, with corresponding ratios of 0.215:0.161:0.15. In addition, PTP-3a demonstrated notable thermal stability and exhibited a triple-helical structure. Moreover, following in vitro fermentation for 48 h, PTP-3a was efficiently utilized, resulting in a reduction in carbohydrate levels, the production of short-chain fatty acids (SCFAs) and pH adjustment. Furthermore, during in vitro fecal microbial fermentation, PTP-3a decreased the relative abundance of Firmicutes while increasing the proportions of Bacteroidetes and Proteobacteria, resulting in a significantly reduced Firmicutes/Bacteroidete s ratio. Additionally, PTP-3a stimulated the growth of beneficial bacteria such as Parabacteroides merdae , Gordonibacter pamelaeae , Bifidobacterium pseudolongum and Parabacteroides distasonis . Importantly, a strong correlation was observed between the production of SCFAs and specific microorganisms. CONCLUSION These findings suggested that PTP-3a has potential as a prebiotic for modulating the gut microbiota. © 2024 Society of Chemical Industry.
研究领域:
真菌学
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生物化学
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微生物学
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营养学
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产品关联
DOI
PubMed
高影响因子
MOLECULAR NEUROBIOLOGY
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2025
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IF 4.3
Wan Yu-hui,Cheng Zeng-jing,Fan Ling-xiang,Yang De-hong,Chen Bing-lin,Chen Xiang-xin,Zhu Qing
Subarachnoid hemorrhage (SAH) is associated with high mortality and morbidity rates. In its early stages, a substantial influx of blood into the subarachnoid space triggers excessive activation of microglia, which markedly contributes to early brain injury (EBI), a pivotal determinant of poor prognosis. Tremella fuciformis polysaccharides (TFPSs), as acidic heteropolysaccharides from the fruiting bodies of Tremella, exhibit robust anti-inflammatory characteristics and many biological properties. Nonetheless, the impact of TFPSs on EBI after SAH has yet to be reported, and the molecular mechanisms underlying these effects remain elusive. We used in vivo and in vitro models to study the effects of TFPSs on microglia post-SAH. Network pharmacology analysis was used to predict the targets of TFPSs and the pathways through which it exerts its therapeutic effects. These predictions were subsequently corroborated through flow cytometry, Western blotting, immunofluorescence staining, enzyme-linked immunosorbent assay (ELISA), and quantitative real‑time polymerase chain reaction, both in vivo and in vitro. After 24 h post-SAH, TFPS-treated mice presented improved neurological function and reduced cerebral edema. TFPSs reversed microglial activation, enhanced phagocytic ability, and reduced neuronal apoptosis. Network pharmacology identified KDR as a potential target of TFPSs, with the P38 MAPK pathway as the downstream pathway. TFPSs attenuated KDR expression, inhibited the P38 MAPK/NF-κB pathway, reduced inflammatory cytokine expression, and increased microglial phagocytic capacity post-SAH. This investigation revealed that TFPSs may ameliorate EBI after SAH, potentially via the regulation of the KDR-mediated P38 MAPK/NF-κB pathway and phagocytic function.
研究领域:
神经科学
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药理学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2025
·
IF 7.7
Jing-Lin He,Bing Li,Yingying Dong,Shudan Zou,Dan Li,Zhong Cao
A green and biocompatible strategy for synthesizing MnO 2 nanoflowers is reported using Tremella fuciformis as a natural reductant and structural template. The resulting MnO 2 was employed as a redox-responsive carrier for constructing a multifunctional DNA nanodevice (X-DMNF) integrating tumor-cell imaging, dual-gene regulation, and photodynamic activity. The X-DNA scaffold incorporated miR-21 recognition elements, Mn 2+ -activated DNAzymes targeting EGR-1 and Survivin mRNA, and a Ce6 photosensitizer. Upon cellular internalization, endogenous glutathione (GSH) triggered MnO 2 degradation, releasing Mn 2+ and restoring fluorescence for miRNA detection. The released DNAzymes catalyzed selective mRNA cleavage, while Ce6 generated singlet oxygen under laser irradiation. In vitro experiments demonstrated efficient miRNA recognition, gene silencing, reactive oxygen species generation, and synergistic cytotoxicity in MCF-7 cells, with minimal off-target effects on normal cells. This work establishes a green and modular approach for constructing GSH-responsive DNA-MnO 2 nanodevices and provides a promising platform for cell-level cancer diagnostics and mechanistic therapeutic studies, pending future in vivo validation.
✓ 已验证
产品关联
DOI
高影响因子
Foods
·
2024
·Vol. 13
·期 10
·1545
·
IF 4.7
Fangwei Liu,Weiwei He,Xiaojun Huang,Junyi Yin,Shaoping Nie
The objective of this study was to investigate the feasibility of the mixture of tremella polysaccharide (TP) and citrus pectin (CP) as an emulsifier by evaluating its emulsifying ability/stability. The results showed that the TP:CP ratio of 5:5 (w/w) could effectively act as an emulsifier. CP, owing its lower molecular weight and highly methyl esterification, facilitated the emulsification of oil droplets, thereby promoting the dispersion of droplets. Meanwhile, the presence of TP enhanced the viscosity of emulsion system and increased the electrostatic interactions and steric hindrance, therefore hindering the migration of emulsion droplets, reducing emulsion droplets coalesce, and enhancing emulsion stability. The emulsification and stabilization performances were influenced by the molecular weight, esterified carboxyl groups content, and electric charge of TP and CP, and the potential mechanism involved their impact on the buoyant force of droplet size, viscosity, and steric hindrance of emulsion system. The emulsions stabilized by TP-CP exhibited robust environmental tolerance, but demonstrated sensitivity to Ca2+. Conclusively, the study demonstrated the potential application of the mixture of TP and CP as a natural polysaccharide emulsifier.
研究领域:
食品科学
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胶体与表面化学
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生物高分子科学
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产品关联
DOI
PubMed
高影响因子
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
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2022
·
IF 3.141
You-wei Ji,Gui-wei Rao,Guang-fa Xie
Here, the extraction of total flavonoids from Tremella fuciformis (TF) by aqueous two-phase extraction combined with ultrasound is presented. The extraction was optimized via a single-variable approach using the variables of mass fraction of ethanol and NaH2PO4, ultrasonication time, and TF sample amount. Response surface methodology (RSM) was then applied to determine the best conditions for extraction using the following three key parameters: ultrasonication time, ethanol mass fraction, and NaH2PO4 mass fraction. The optimum conditions were as follows: an aqueous two-phase system of ethanol/NaH2PO4, an ethanol mass fraction of 23%, a NaH2PO4 mass fraction of 27.18%, and an extraction time of 8.25 min. Under these conditions, the maximum extraction rate of total flavonoids was 0.158 mg/g. The scavenging rates for superoxide anion and hydroxyl radical, as well as the antioxidant activity of total flavonoids in TF, were studied. Total flavonoids from TF showed some scavenging ability, but the scavenging effects were lower than those of vitamin C (Vc) at the same concentrations. Nevertheless, ultrasound-assisted two-phase extraction was considered an efficient and rapid method that can be used to extract total flavonoids from TF.
研究领域:
药学
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食品科学
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生物技术
·
分析化学
✓ 已验证
DOI
高影响因子
MOLECULES
·
2023
·Vol. 28
·期 10
·4018
·
IF 4.6
Lingna Xie,Guangrong Liu,Zebin Huang,Zhenyuan Zhu,Kaiye Yang,Yiheng Liang,Yani Xu,Lanyue Zhang,Zhiyun Du
Anti-tumor activity ofTremella fuciformispolysaccharides (TFPS) has been widely reported, but its mechanism remains poorly understood. In this study, we established an in vitro co-culture system (B16 melanoma cells and RAW 264.7 macrophage-like cells) to explore the potential anti-tumor mechanism of TFPS. Based on our results, TFPS exhibited no inhibition on the cell viability of B16 cells. However, significant apoptosis was observed when B16 cells were co-cultured with TFPS-treated RAW 264.7 cells. We further found that mRNA levels of M1 macrophage markers including iNOS and CD80 were significantly upregulated in TFPS-treated RAW 264.7 cells, while M2 macrophage markers such as Arg-1 and CD 206 remained unchanged. Besides, the migration, phagocytosis, production of inflammatory mediators (NO, IL-6 and TNF-α), and protein expression of iNOS and COX-2 were markedly enhanced in TFPS-treated RAW 264.7 cells. Network pharmacology analysis indicated that MAPK and NF-κB signaling pathways may be involved in M1 polarization of macrophages, and this hypothesis was verified by Western blot. In conclusion, our research demonstrated that TFPS induced apoptosis of melanoma cells by promoting M1 polarization of macrophages, and suggested TFPS may be applied as an immunomodulatory for cancer therapy.Keywords:Tremella fuciformispolysaccharide;immunoregulation;co-culture;macrophages;B16
研究领域:
免疫学
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肿瘤学
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药理学
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细胞生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PROCESS BIOCHEMISTRY
·
2023
·
IF 4.4
Xi-ge Li,Feng-yi Zhang,Chao-xian Jiang,Jun Jiang,Yan-hui Hou,Jia-wei Zhang
To investigate the composition, structure, and physical and chemical properties of Tremella fuciformis polysaccharide (TFPS), four new polysaccharide fractions (TFPS1, TFPS2, TFPS3, and TFPS4) were successfully isolated from hot water-extracted crude polysaccharides using a DEAE-52 cellulose column. Their structures were analyzed using FT-IR, SEM, HPGPC, monosaccharide composition, Congo red and I 2 -KI experiments. TFPS1 was found to be a heteropolysaccharide with a molecular weight of 742 Da for the major polysaccharide fragment. TFPS2, TFPS3, and TFPS4 were identified as single-component homogeneous polysaccharides with molecular weights of 350774 Da, 622675 Da, and 624724 Da, respectively. Glucose was the dominant monosaccharide of TFPS1 and TFPS2, while mannose was the major monosaccharide of TFPS3 and TFPS4. The results showed that TFPS1 and TFPS2 displayed a stable triple helix structure in aqueous solution, while TFPS3 and TFPS4 had longer side chains and more branched chains. Additionally, all four TFPSs showed in vitro antioxidant activity, as well as significant cholesterol binding and sodium taurocholate binding abilities.
研究领域:
生物化学
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药理学
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食品科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）