Yinping Li,Qing He,Xiaoqing Zhu,Zhufeng Geng,Yi Wang,Jin Li
Daphne altaica Pall. is an important plant in traditional Chinese medicine. Mineral elements play an important role in the formation of active ingredients in medicinal plants. In this study, 14 mineral elements were quantitatively determined by inductively coupled plasma emission spectrometry using the roots, stems and leaves of D. altaica. The distribution variance of mineral elements between different organs were analyzed by statistical analysis methods such as single factor analysis of variance (ANOVA), correlation analysis (CA), principal component analysis (PCA) and cluster heat map. The results show that the mineral element contents in corresponding parts of D. altaica exhibited significant variation, with the order from highest to lowest being K > Ca > Mg > Fe > Al > Mn > Na > Zn > Sr > Cr > Ni > Cu > Pb > Mo. Among them, the highest content of mineral elements are K, Ca, Mg, Mn, Cu and Mo in leaves, and the stems contains more Zn, Sr, Cr and Ni, while the content of mineral elements Fe, Al, Na and Pb in roots is much higher than the other two organs. The ANOVA revealed significant variations in the mineral element content across different regions of D. altaica (p < 0.05). Based on single factor analysis of variance, different parts of D. altaica had different fingerprint of mineral elements, and the concentration of the same mineral elements in different parts had significant difference (p < 0.05). The principal component analysis (PCA) model showed that the cumulative variance contribution rate of first two principal components was 99.10%, in which Mn, Mg and Ca have higher load values, which is consistent with the results of bi-plot and cluster analysis. These results indicated that there were great differences in absorption and utilization of mineral nutrient elements between roots, stems and leaves of D. altaica, and there was significant correlation among mineral elements, indicating that there was a complex synergistic and antagonistic effect among the elements. At the same time, the discriminant analysis of the characteristic elements provided a scientific basis for the quality control of D. altaica.
Liu Qing,Xiao Xu-Hui,Hu Liu-Bing,Jie Hui-Yang,Wang Ying,Ye Wen-Cai,Li Man-Mei,Liu Zhong
Anemone flaccida Fr. Schmidt (Ranunculaceae) (Di Wu in Chinese) is used to treat punch injuries and rheumatoid arthritis (RA). Our previous report has shown that crude triterpenoid saponins from Anemone flaccida exhibited anti-arthritic effects on type II collagen-induced arthritis in rats. Furthermore, anhuienoside C (AC), a saponin compound isolated from A. flaccida, was observed to suppress the nitric oxide production in lipopolysaccharide (LPS)-treated macrophage RAW 264.7 cells. In this study, we examined the effects of AC on the prevention and treatment of collagen-induced arthritis in a mouse model and evaluated the potential mechanisms involved. We observed that oral administration of AC significantly suppressed the paw swelling and arthritic score, decreased the body weight loss, and decreased the spleen index. Improvement in the disease severity was accompanied by the reduction of cluster of differentiation 68 (CD68)-positive cells in the ankle joint and inhibition of the pro-inflammatory cytokine tumor necrosis factor alpha (TNF-α) in the synovium of the joint. Mechanistic studies indicated that AC exerted its anti-inflammatory activity by inhibiting the mRNA expression levels of inducible nitric oxide synthase, cyclooxygenase-2, TNF-α, interleukin (IL)-1β, and IL-6 and by suppressing the production of inflammatory cytokines such as TNF-α, IL-1β, and IL-6 in LPS-treated RAW 264.7 cells. AC also blocked the LPS-induced activation of the extracellular signal-regulated kinase, c-Jun N-terminal kinase, and p38 mitogen-activated protein kinase pathways. Additionally, the LPS-induced activation of nuclear factor kappa-B (NF-κB) was significantly suppressed by AC treatment, as indicated by down-regulation of TLR4 and inhibition of the nuclear translocation of NF-κB p65 and by activation and degradation of the inhibitor of kappa B. These findings indicated that AC has a great potential to be developed as a therapeutic agent for human RA.
危险品化学品经营许可证（带存储）