Nature Communications
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2025
·Vol. 16
·期 1
·1-24
·
IF 15.7
Zheng Liu-Ting,Yan Zeng-Shuai,Li Xin-Yue,Chang Jia-Jia,Tan Xiao-Qi,Wang Yu-Xing,Ding Hong-Ming,Liu Qin,Ma Yu-Qiang,Huo Da
Membraneless organelles form by phase separation and regulate cell behavior. We show that cholesterol-patterned AuNPs program nanomaterial-induced stress granules (NSGs) by lowering G3BP1 condensation barriers through a solid–liquid–liquid triphasic sequence: nanomaterials recruit hnRNPC, which then engages G3BP1 to nucleate gel-like condensates. We map NSG microenvironments (temperature, polarity, pH, and proteasome activity), uncover dual disassembly—a slow VCP/19S-dependent route and a rapid SUMO/20S-dependent backup—and show that NSGs remodel chemo-plasticity: they mitigate doxorubicin/cisplatin toxicity in normal tissues yet sensitize tumors to nocodazole in vivo. Local induction and selective dissolution of NSGs thus offers a strategy to decouple efficacy from toxicity. Our results establish design rules linking nanomaterial surface chemistry to condensate programming and provide actionable levers to steer therapeutic outcomes.
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MICROCHEMICAL JOURNAL
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2025
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IF 5.1
Meiyun Shang,Juan Hu,Mengjia Shi,Yunpeng Fan,Yuan Zou,Yong Wang,Jinhong Guo,Cuiping Mao
Anti-cyclic citrullinated peptide (anti-CCP) antibodies are key biomarkers for rheumatoid arthritis and vital for its early diagnosis and monitoring. Conventional chemiluminescence immunoassays (CLIA) rely on streptavidin-functionalized magnetic beads (SA-MBs), which suffer from limited binding capacity and require labor-intensive chemical conjugation steps. Here, we report a MBs carrier modified with zirconium (Zr)-based metal-organic frameworks (MB@MOF), enabling rapid and efficient SA functionalization via one-step physicochemical adsorption. Compared to the conventional EDC/NHS method, our approach shortens the modification time by at least sixfold and eliminates the need for chemical crosslinkers. The MB@MOF carrier was constructed by anchoring Zr-MOF nanoparticles, synthesized with TCPE as the organic ligand, onto MBs. This carrier supports stable and dense SA loading while preserving native SA activity, thereby allowing highly sensitive detect anti-CCP antibodies. The developed MB@MOF carrier-based CLIA method achieved a detection limit of 0.1 RU/mL and a linear range of 0.5–200 RU/mL. Clinical validation using 36 clinical serum samples showed strong agreement with results from standard clinical analyzers, achieving an overall diagnostic accuracy of 91.7 %. This strategy significantly simplifies the assay workflow and holds strong potential for high-performance and rapid diagnostics of rheumatoid arthritis in clinical application.
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ACS Sensors
·
2025
·
IF 9.1
Baiqi Cui,Xiaoyin Liu,Jinbiao Ma,Jingyu Wu,Yunxiao Wang,Haiying Ding,Di Wang,Qingjun Liu,Fenni Zhang
Phase-sensitive surface plasmon resonance (P-SPR) techniques offer superior sensitivity compared to conventional intensity-based SPR methods. However, current implementations, such as interference-based and polarization-based phase measurements, often face trade-offs between optical complexity and phase resolution, and typically lack access to full polarization information. Here, we report a compact and versatile Stokes vector-based polarization SPR imaging (Sp-SPRi) system that addresses these limitations through full Stokes vector analysis of imaging light after polarization modulation. This analysis enables the simultaneous acquisition of phase and multiple polarization parameters for enhanced molecular measurement. The Sp-SPRi system achieves a high phase sensitivity of 1.80 × 10–7 refractive index units (RIU), supporting kinetic, label-free detection and quantification of biomolecular interactions. We demonstrate its performance through kinetic measurements of protein binding, small molecule interactions, and in situ glycoprotein measurement of single cells. With its simplified optical configuration, microfluidic integration, and high-throughput imaging capabilities, Sp-SPRi provides a powerful platform for multidimensional biosensing. This work broadens the analytical scope of P-SPR, offering a robust and accessible approach for applications in point-of-care diagnostics, early disease detection, and drug screening.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2026
·
IF 8.3
Tingling Lin,Shiqing Li,Yi Huang,Shuncong Zhong,Fuwei Sun,Yujie Zhong,Qiuming Zeng,Rongkun Yuan,Yonglin Huang
Plasmonic resonators represent an emerging toolkit in biosensing by focusing electromagnetic waves within strongly enhanced electric field hotspots to facilitate light-matter interaction. However, the limited efficiency of field enhancement and the low spatial overlap between analytes and plasmonic hotspots impose fundamental limitations on achieving high-performance molecular assays. In this work, a terahertz (THz) biosensor for specific detection that allows molecules in solution to readily access predefined strong hotspot regions is presented. We construct heterogeneous nanogap structures by introducing a 20 nm-thick SiO 2 nanofilm to efficiently localize THz waves into the gaps providing over 300-fold field enhancement and optimal spatial matching with molecules. The sulfhydryl compounds are utilized to form Au-S bonds to cover large areas of the gold surface, preventing the aggregation of target molecules in the non-hotspot regions. Furthermore, the exposed silica surfaces within the nanogaps are functionalized with a biotin monolayer via silane coupling, enabling targeted active capture trace amounts of free streptavidin molecules in solution for specific detection at low concentrations down to 150 fM. This approach provides a valuable toolkit for ultra-sensitive biochemical detection and opens new avenues for the development of photonic devices in multifunctional, real-time, and on-chip sensing.
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ACS Applied Nano Materials
·
2026
·
IF 5.5
Chuwen Song,Rong Huang,Peiyong Song,Ke Shi,Jianming Li,Yiyang Lin
Nucleic acids have long been recognized for their essential role in storing and transmitting genetic information. Inspired by the functional and recognition properties of DNA and RNA, we designed a class of nucleobase-appended bottlebrush polymers to serve as advanced surface ligands for stabilizing plasmonic nanomaterials. Leveraging multivalent metal–nucleobase interactions, these bottlebrush polymers provided robust protection to colloidal nanomaterials, preventing undesired aggregation under harsh conditions including high salt concentrations, broad pH ranges, freeze–thaw cycles, and elevated temperatures. Additionally, the surface attachment of polymer ligands enabled the phase transfer of nanoparticles from aqueous media to organic media. The customizable design of these polymeric ligands facilitates the further functionalization of ligand-protected nanoparticles. Compared to conventional thiol-based ligands, the nucleobase-functionalized bottlebrush ligands exhibited a superior ability to offer colloidal stability under harsh conditions, owing to their multivalent interactions. This unique stability will open opportunities for the development of advanced biosensing, imaging, and therapeutic applications.
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MICROCHEMICAL JOURNAL
·
2026
·
IF 5.1
Xiaoshuo Li,Xinyue Ma,Zhuolin Fu,Zongwen Jin,Chunyan Sun,Xue Qiu,Jiajia Guo
The global spread of viral infections, particularly highlighted by the COVID-19 pandemic, has underscored the critical demand for rapid, sensitive, and widely accessible diagnostic tools. While polymerase chain reaction (PCR) remains the gold standard for virus detection due to its high sensitivity and specificity, its reliance on complex instrumentation and lengthy processing times makes it unsuitable for point-of-care testing (POCT). In contrast, lateral flow immunoassays (LFIA) are commonly used in POCT for their simplicity and rapid results, yet they often exhibit low sensitivity and limited accuracy, particularly when detecting low concentrations of viral antigens. In this study, we reported an ultrasensitive duplex lateral flow immunoassay (UdLFIA) that integrated hybridization chain reaction (HCR) amplification and dual-functionalized gold nanoparticles (AuNPs) to achieve both high sensitivity and accuracy. To demonstrate its capabilities, we applied the UdLFIA for the simultaneous detection of two SARS-CoV-2 antigens-the nucleocapsid (N) protein and the receptor-binding domain (RBD) of the spike protein. This model confirmed UdLFIA's benefits, enhancing diagnostic accuracy by reducing false negatives when targeting a single antigen. The UdLFIA achieved a limit of detection (LOD) of 0.65 ng/mL for the N protein and 0.99 ng/mL for the RBD protein, representing a 10–20 folds improvement in sensitivity with HCR amplification. The good selectivity of the UdLFIA was demonstrated by comparing the measurement with closely related viruses. Additionally, the 20 min measurement time makes it well-suited for large-scale screening and POCT settings. The UdLFIA platform represents a step toward bridging between high-sensitivity laboratory diagnostics and accessible point-of-care testing. By combining the simplicity and speed of LFIA with enhanced sensitivity and accuracy, UdLFIA may support scalable and cost-effective testing after further validation in clinical and real-world settings for both pandemic response and routine viral screening, addressing the limitations of current POCT technologies.
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BIOSENSORS & BIOELECTRONICS
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2026
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IF 11.8
Feng Lin,Wanglong Li,Xinhuang Hou,Wei Lin,Xiaoling Lai,Xiaoqi Zheng,Luyao Li,Wenqian Sun,Jun Lin,Pinjie Lin,Feilin Lian,Fanggang Cai,Kun Wang,Yiquan Dai
Urinary extracellular vesicles (uEVs) provide noninvasive biomarkers for liquid biopsy owing to their ability to reflect disease-associated molecular alterations. However, the accurate analysis of low-abundance uEV surface proteins in complex biological matrices remains a major analytical challenge. Here, we report a proximity-activated dual-cascade (PADC) biosensing platform that integrates a target-responsive 3D DNA machine with a nanozyme-mediated ratiometric transduction circuit for high-fidelity multiplexed phenotyping of uEVs. Upon dual-recognition of target uEVs, the DNA machine undergoes proximity-induced spatial confinement on individual magnetic particles, creating a localized catalytic microenvironment that markedly enhances DNA walking kinetics and signal amplification efficiency. Coupled with an enzyme-nanozyme redox cascade, this confined catalytic process generates a robust ratiometric fluorescence output for sensitive and reliable molecular profiling. Benefiting from the spatially confined amplification architecture, the proposed platform enables ultrasensitive multiplexed analysis of CD63, EGFR, and PAK6 with detection limits down to 4.3 × 10 4 , 2.2 × 10 4 , and 1.7 × 10 3 particles/mL, respectively. Importantly, the target-uEV response was approximately six-fold higher than those generated by common urinary interferents or their mixture, demonstrating high analytical selectivity in complex urine matrices. To further evaluate its universality for multi-disease screening, multidimensional molecular profiling combined with machine learning algorithms was performed on clinical uEV samples collected from healthy controls, Diabetes Mellitus (DM) patients, and prostate cancer (PCa) patients. The optimal classification model achieved an overall multiclass diagnostic accuracy of 91% for distinguishing healthy, DM, and PCa groups. This work establishes a proximity-confined catalytic biosensing strategy for multiplexed uEV analysis and demonstrates its potential for uEV-based liquid biopsy and proof-of-concept disease stratification.
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MICROWAVE AND OPTICAL TECHNOLOGY LETTERS
·
2026
·Vol. 68
·期 7
·e70699
·
IF 1.1
Zhengqi Wang,Yangliu Zhou,Bo Qian,Mingya Yang,Xiaonan Zhang,Benli Yu,Sheng Zhou
Early diagnosis of cancer is the key to improving the cure rate, but traditional detection methods (such as tissue biopsy and imaging examinations) often have problems such as strong invasiveness, insufficient sensitivity, or high cost. In this work, an ultrasensitive microfiber interferometer biosensor is proposed for cancer cell detection. An ultra-high refractive index (RI) sensitivity of 15225.665 nm/RIU was achieved by cascading two taper-in-taper structured fiber (TITF). TITF that is functionalized with biotin was employed to identify the biotin receptor protein (streptavidin) and the cancer cell exhibiting overexpression of surface biotin receptors (Human Hepatocellular Carcinoma Cells, HepG2). The results of the experiments indicate that in the standard buffer sample, the detected concentrations of streptavidin (SA) were 11, 23, 46, and 92 pM, respectively, with a detection limit of 1.4 pM. The log-linear concentrations of HepG2 cells are 10, 10 2 , 10 3 , and 10 4 cells/mL, respectively, with a detection limit of 4 cells/mL. The specificity test revealed that TITF showed a lack of sensitivity to normal cells, particularly human acute monocytic leukemia cells (THP-1) and human colorectal adenocarcinoma epithelial cells (HCT116). The proposed biosensor has good selectivity and sensitivity, providing a feasible method for the early diagnosis of cancer.
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ACS Sensors
·
2026
·
IF 10.9
Wang Zilun,Zhao Zeyu,Jiang Xiangwei,Fu Muqing,Zheng Zhiwen,Duan Xuexin,Xue Qiannan,You Rui
The analysis of exhaled breath condensate (EBC) for respiratory pathogen detection is fundamentally limited by the extreme dilution of target analytes, leading to insufficient sensitivity in point-of-care platforms. Here, we present a paradigm-shifting solution inspired by the efficient multi-stage capture mechanism of the human respiratory system. We engineered a tri-phasic hierarchical interface that orchestrates a synergistic cascade for viral enrichment and detection: (1) microscale pillar arrays that mimic bronchial bifurcations to direct airflow and enhance particle-surface collisions via inertial impaction; (2) a TiO 2 nanowire forest that emulates the mucin network, providing a high-surface-area scaffold for analyte retention; and (3) a functionalized polymer coating for specific antibody-antigen recognition. This bio-inspired architecture, optimized through computational fluid dynamics and fabricated via wafer-level MEMS processes, achieved a 3.6-fold increase in condensate collection and a 500% enhancement in capture-site density. When integrated into a portable fluorescence detection system, the platform demonstrated high specificity (>95%) and detected SARS-CoV-2 pseudovirus down to 3 TU/mL under the tested simulated-breath workflow. Critically, the binding kinetics, analyzed using the Hill model, further supports a saturation-like, heterogeneous capture behavior of the hierarchical interface. This work not only delivers a powerful tool for non-invasive diagnostics but also establishes a generalizable biomimetic design paradigm for next-generation biosensors targeting ultra-dilute biomarkers.
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Journal of the American Chemical Society
·
2026
·
IF 16.6
Xinwei Zhang,Chonglu Li,Mingzhe Yan,Yuting Wang,You Dou,Tuotuo Zhang,Shuo Zheng,Hanying Wang,Junrong Li,Yao Sun
Precise profiling of multiple circulating tumor DNA (ctDNA) is hindered by the challenge of resolving single-nucleotide variants (SNVs) and their trace abundance. Herein, we present an integrated SERS platform that combines rationally molecularly engineered nanotags with a novel microturbulent electrofluidic interface for multiplexed SNV-level profiling of lung cancer ctDNAs. In particular, the SERS nanotags incorporate (1) a de novo isomeric Raman reporter library that systematically tailors vibrational signatures through molecular symmetry and electronic effects, (2) hollow hyperbranched Cu2O/CuO@Ag heterostructures engineered to suppress carrier recombination for maximized electromagnetic–chemical signal enhancement, and (3) rationally designed dCas9–sgRNA units that confer single-nucleotide discrimination. To overcome diffusion-limited mass transport and further boost the signal, we fabricated nanopebble-structured asymmetric microelectrodes on aluminum foil. Unlike a conventional smooth interface, this unique topography induces powerful microturbulence-enhanced electrohydrodynamics (mt-EHD) that disrupts the interfacial depletion layer to accelerate target delivery while simultaneously offering high-density “hot spots” within interpebble gaps for secondary Raman signal amplification. This integrated system achieved the simultaneous quantification of six ctDNA mutations in 131 clinical samples. By leveraging machine learning algorithms, the platform enables accurate diagnosis, prognostic stratification, and dynamic treatment monitoring, establishing an integrated chemical–electrofluidic strategy for precision liquid biopsy.
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JOURNAL OF HAZARDOUS MATERIALS
·
2025
·
IF 11.3
Kun Fu,Jia Zheng,Yichao Zhang,Chang Liu,Wenxiang Zhang,Siyu Chen
Current analytical methodologies for phthalic acid esters (PAEs) face significant challenges in achieving rapid on-site detection and the discriminative identification of individual congeners. This study utilizes Capture-SELEX technology to identify aptamers specifically recognizing dibutyl phthalate (DBP), di-n-octyl phthalate (DNOP), and diethyl phthalate (DEP). Fluorescence-based affinity assays revealed that these aptamers possess dissociation constants (Kd) of 1.577, 0.889, and 0.173 μM for their respective targets. Molecular docking simulations, coupled with base mutation analyses, were conducted to predict potential binding interfaces. By integrating these aptamers with the previously characterized di(2-ethylhexyl) phthalate (DEHP)-aptamer, a gold nanoparticle (AuNPs)-mediated visual detection platform was developed to validate the efficacy and potential of these aptamers for subsequent applications. The calculated limits of detection (LOD) for DBP, DEHP, DNOP, and DEP using this system are 17.64, 2.32, 25.68 and 22.01 ng/mL, respectively. Furthermore, a lateral flow strip was designed to enable the simultaneous and on-site multiplex detection of the four PAEs. This strip achieved LODs of 12.54, 14.95, 14.93 and 30.28 ng/mL for DBP, DEHP, DNOP, and DEP, respectively. A comparative evaluation using LC–MS on identical samples revealed quantitative concordance within a ratio range of 94.18 %–110.43 %, highlighting the robust analytical performance, detection efficiency, and translational potential of the strip for widespread application in various PAE testing scenarios.
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Small
·
2025
·
IF 12.1
Yixuan Yang,Xia Liu,Lanlan Jia,Jiabo Wang,Qianhui Wu,Minglei Zhang,Yusi Bu,Xiaoyu Xie
Enzyme-catalyzed micro/nanomotors exhibit significant promise in drug delivery and sensing due to the exceptional biocompatibility, adaptability, and capacity to employ endogenous fuels. Nevertheless, the flow field generated by the catalytic reaction of enzymes exposed to the outside may considerably impair the recognition ability of surface-connected functional components, such as aptamers. Here, advantage is taken of cell membrane coating technology to create biomimetic micromotors that selectively distributed glucose oxidase (GOx) and employ aggregation-induced emission sensing to quickly capture and visualize circulating tumor cells (CTCs). Once the cell membrane selectively filters glucose, the flow field created by GOx is restricted within the intramembrane cavity. This design produces the required driving force while reducing interference on the exterior surface recognition function. By spatially segregating functional components, these biomimetic micromotors achieve CTCs collection in less than a min, enable real-time in situ detection. The proposed strategy demonstrates the potential of spatially segregated biomimetic micromotors for rapid CTCs enrichment and real-time sensing, which may inspire further development of multifunctional platforms in liquid biopsy applications.
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