Analytical Methods
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2019
·Vol. 11
·期 15
·2033-2040
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IF 2.378
Yimei Ma,Xiaoyong Jin,Yonglei Xing,Gang Ni,Juan Peng
In this study, an NAND molecular logic gate based on molecularly imprinted functionalized quantum dot (QDs@MIPs) composites was constructed for the simultaneous detection of streptomycin sulphate (SS) and kanamycin sulfate (KS). To achieve this goal, two kinds of CdTe QDs with green (QDG) and red emission (QDR) colours were prepared. Then, QDG@MIPs were used for the detection of SS in the concentration range of 3.00–118 μM, and the limit of detection (LOD) was 0.22 μM. QDR@MIPs were used for the estimation of KS in the concentration range of 3.00–105 μM, and the limit of detection (LOD) was 0.24 μM. The molecularly imprinted dual-emission quantum dot composites (DEQDs@MIPs) exhibited two emission peaks at 525 nm and 625 nm with excitation at 360 nm. DEQDs@MIPs were used as fluorescent probes for the rapid and simultaneous detection of SS and KS. In this system, the input of SS and KS and the output of the fluorescence value coincided with the NAND logic gate; thus, the NAND molecular logic gate can be constructed using DEQDs@MIPs for the simultaneous detection of SS and KS.
研究领域:
分析化学
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生物医学工程
·
纳米技术
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GLYCOBIOLOGY
·
2017
·Vol. 27
·期 9
·861-867
·
IF 3.112
Qiu Yuanhao,Hou Yilin,Sun Feifei,Chen Peng,Wang Dongdong,Mu Haibo,Zhang Xiaoli,Ding Kan,Duan Jinyou
Antibiotics such as β-lactams and aminoglycosides are often subtherapeutic to intracellular infections due to their high hydrophilicity, resulting in low effectiveness against intracellular pathogens and the emergence of antibiotic resistance. Here we reported that an endogenous aminoglycan, hyaluronic acid could be an effective carbohydrate carrier of the aminoglycoside antibiotic, streptomycin against intracellular pathogens. This conjugation could enhance phagocytic activity, and facilitated the entry of streptomycin into host cells via a CD44-mediated pathway. It appeared that this conjugate could clear intracellular bacteria in phagocytic or nonphagocytic cells in a short-term therapy (4 h) at a lower effective dose. In addition, this conjugate was more efficient in reducing bacteria burden in an in vivo acute infection model than streptomycin did. Interestingly, subcutaneous injection of this conjugate at an excess amount had undetectable side effects such as nephrotoxicity. These results suggested that hyaluronic acid might be an efficient Trojan horse for the delivery of hydrophilic antibiotics to deal with intracellular infections.
研究领域:
药理学
·
微生物学
·
药物递送系统
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PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2022
·
IF 9.136
Yingying Liu,Jiao Du,Haowen Wu,Chang Cong,Haifeng Zhang,Jixiao Wang,Zhi Wang
Nanofiltration (NF) membrane with excellent dye/salt permselectivity and anti-fouling performance is highly desired for the separation of dye/salt mixture in dye wastewater treatment . In this work, the antibacterial NF membrane with high permselectivity and anti-fouling performance was prepared by using a hydrophilic antibiotic, streptomycin (SM) with abundant amino and hydroxyl groups, as the aqueous monomer via interfacial polymerization (IP) with trimesoyl chloride (TMC). The as-prepared antibiotic membrane had a hydrophilic and highly smooth surface, which was beneficial to the membrane anti-fouling performance. The membrane performance was optimized by tuning the concentration of SM, and the optimized membrane with desirable water permeance of 23.5 L·m −2 ·h −1 ·bar −1 had excellent dyes rejections (the rejections of Direct red 23 (DR 23), Congo red (CR), Acid red 66 (AR 66), Victoria blue B (VB), and Chrome black T (CBT) were 99.7%, 99.6%, 99.0%, 99.5%, 99.7%, respectively) and low salts rejection (the rejections of NaCl and Na 2 SO 4 were 1.9% and 4.5%, respectively). The optimized membrane also showed excellent separation performance to salt/dye mixed solutions and operational stability. This newly streptomycin-based NF membrane showed excellent application potential in the dye wastewater treatment.
研究领域:
环境科学
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材料科学
·
生物医学
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高影响因子
BIOSENSORS & BIOELECTRONICS
·
2017
·
IF 7.78
Bixia Lin,Ying Yu,Yujuan Cao,Manli Guo,Debin Zhu,Jiaxing Dai,Minshi Zheng
s The rapid detection of antibiotic residual in everyday life is very important for food safety. In order to realize the on-site and visual detection of antibiotic, a POCT method was established by using digital image colorimetry based on smartphone. Streptomycin was taken as the analyte model of antibiotics, streptomycin aptamer preferentially recognized analyte, and the excess aptamer hybridized with the complementary DNA to form the dsDNA . SYBR Green I combined with the dsDNA and then emitted obvious green fluorescence, thus the fluorescence intensity decreased with the increasing of streptomycin concentration. Then a smartphone-based device was constructed as the fluorescence readout. The smartphone camera acquired the images of the fluorescence derived from the samples, and the Touch Color APP installed in smartphone read out the RGB values of the images. There was a linear relationship between the G values and the streptomycin concentrations in the range of 0.1–100 µM. The detection limit was 94 nM, which was lower than the maximum residue limit defined by World Health Organization. The POCT method was applied for determining streptomycin in chicken and milk samples with recoveries in 94.1–110%. This method had the advantages of good selectivity , simple operation and on-site visualization.
研究领域:
食品安全
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生物医学检测
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分析化学
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PubMed
高影响因子
MICROCHIMICA ACTA
·
2017
·Vol. 184
·期 8
·2941-2948
·
IF 4.58
Han Cong,Li Rongguo,Li Hui,Liu Su,Xu Chenggong,Wang Jingfeng,Wang Yu,Huang Jiadong
The authors describe a label-free electrochemical aptasensor for ultrasensitive and highly specific detection of the antibiotic based on cascade enzymatic recycling couple with DNAzyme amplification. The assay involves two sequential reactions: the first reaction is a λ exonuclease-assisted cyclic digestion reaction triggered by target-aptamer binding. The second reaction is a nicking endonuclease-aided cyclic nicking reaction, which produces a large amount of G-rich nucleic acid segments. These form a G-quadruplex/hemin complex in the presence of K(I) ions and hemin. Because the G-quadruplex/hemin complex acts as a horseradish peroxidase-mimicking DNAzyme with excellent redox activity, the electrochemical signal transduction is accomplished due to the electroreduction of H 2 O 2 . It appears that this work is the first example that cascade enzymatic recycling coupled to DNAzyme amplification is used for antibiotic detection. The aptasensor was applied to the quantitation of kanamycin and gave a response that is linear in the 1 pM to 10 nM kanamycin concentration range, with a detection limit as low as 0.5 pM. The working voltage (vs. Ag/AgCl) at which data can be acquired best is −0.35 V. The assay offers the advantages of remarkably improved sensitivity, use of affordable instrumentation, and simplified operation without the need for electrochemical labeling or addition of labile reagents. Thus, cascade enzymatic recycling coupled to DNAzyme amplification represents a versatile platform for highly sensitive and specific antibiotics detection.
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研究领域:
分析化学
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生物医学工程
·
分子生物学
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高影响因子
NEW JOURNAL OF CHEMISTRY
·
2023
·Vol. 47
·期 14
·6739-6748
·
IF 3.3
Chuanyu Zhu,Zhongmin Feng,Yupu Meng,Min Wang,Zhuqing Wang
In this study, a novel sodium alginate–streptomycin sulfate composite aerogel (Alg–Stre) was synthesized by using chemical grafting and vacuum freeze-drying techniques to remove heavy metal ions from water. The experimental results show that the prepared Alg–Stre can effectively remove heavy metal ions such as Pb2+ and Cu2+ from water. Among these ions, Alg–Stre demonstrated the best removal effects for Pb2+ (99.8%, 0.1 mM) and Cu2+ (94.6%, 0.1 mM) in water. The utmost adsorption capacities of Alg–Stre for Pb2+ and Cu2+ are 280 mg g−1 and 160.2 mg g−1, respectively, which are competitive with those of most reported Pb2+- or Cu2+-sorbents. In addition, the adsorption abilities of Alg–Stre for Pb2+ and Cu2+ remained stable in solution pH values of 3.0 to 6.0 and at ambient temperatures of 20 °C to 50 °C. The simple preparation process, high removal efficiency, suitability for different environments and conditions, and recyclability of Alg–Stre confirm the broad application prospects of this material in the field of sewage disposal.
研究领域:
环境科学
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材料科学
·
污水处理
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高影响因子
ANALYTICA CHIMICA ACTA
·
2020
·
IF 5.977
Yanni Luo,Xuecai Tan,David J. Young,Quanyou Chen,Yuehui Huang,Defen Feng,Chenhao Ai,Yan Mi
In photoelectrochemical sensor (PEC sensor), sensitivity and selectivity are two essential factors which are determined by photosensitive of materials and identification of elements. Herein, a novel PEC aptamer sensor for streptomycin-specific detection was developed, with which the visible-light-active TiO 2 /BiOI/BiOBr heterostructure and aptamers were employed as photoactive material and bio-identification elements, separately. The combination of an appropriate amount of TiO 2 with BiOI/BiOBr enhanced the photocurrent response, and thus is beneficial to the construction of PEC sensors. In addition, the one-pot synthesis of TiO 2 /BiOI/BiOBr has the advantage of being environmentally-friendly. Under optimized conditions, the photocurrent response of aptamer/TiO 2 /BiOI/BiOBr/ITO is linear with SRT concentration from 0.05 to 150 nM, and the detection limit (S/N = 3) is as low as 0.04 nM. This novel PEC sensing strategy provided an ultra-sensitive sensor with high selectivity and stability for SRT detection.
研究领域:
生物传感技术
·
材料科学与工程
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PubMed
高影响因子
IEEE SENSORS JOURNAL
·
2024
·
IF 4.3
Jie Liu,Yinping Miao,Xiaolan Li,Mingpan Bi,Yanxi Wang,Yang Zhang
Streptomycin sulfate (STS) is one of the major sources of contamination in water. Excessive intake of STS can be a serious health hazard to humans, causing vestibular and auditory nerve damage, nephrotoxicity, myasthenia gravis or Parkinson’s disease. To address this, we propose an STS concentration sensor leveraging multiwalled carbon nanotubes (MWCNTs) and the vernier effect of a microfiber coupler (MFC). The vernier effect of MFC is caused by the interference of two supermodes in two orthogonal polarizations in a refringent optical fiber, and the high sensitivity can be achieved directly by monitoring the vernier envelope. MWCNTs can adsorb more biomolecules to enhance the light absorption of analytes to further improve the detection sensitivity. The influence of MFC structure parameters on sensor performance is analyzed by simulation and verified by experiment. Theoretical analyses and experimental results indicate that for specific diameter MFC, the sensor will have better performance. The sensor exhibits a remarkably high sensitivity of 1577.5 nm/(mg/ml) in the detection range of 0.02-0.1 mg/ml, namely sensitivity amplification factor nearly one order of magnitude, and the sensor achieves an ultralow limit of detection of 12.68 ng/ml. The vernier effect of MFC and the modification of MWCNTs effectively enhance the sensitivity of the STS sensor. The device does not require complex pretreatment or antibody preparation, making it highly promising for applications in food safety, environmental pollution, and health monitoring.
研究领域:
生物医学工程
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环境科学
·
光学传感
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高影响因子
JOURNAL OF DAIRY SCIENCE
·
2020
·
IF 3.333
Zile Wang,Yuanze Sun,Demei Liang,Yuyang Zeng,Shuang He,Ghulam Mujtaba Mari,Tao Peng,Haiyang Jiang
ABSTRACT Antibiotic residues are major contaminants in milk because of their use in agriculture and animal husbandry. In particular, streptomycin, an aminoglycoside antibiotic, is a potential risk to consumers because of its ototoxicity, anaphylaxis, and growth inhibition. Herein, monoclonal antibodies for streptomycin were conjugated with europium microspheres to serve as detection probes for the development of a chromatographic time-resolved fluoroimmunoassay to detect streptomycin residues in milk. The method had a low detection limit of 0.58 µg/kg, a linear range of 0.8 to 6.25 μg/kg, and substantial recovery, from 85.6 to 108.3%. It showed slight cross-reactivity with another aminoglycoside analog. Strong correlations between the results of established chromatographic time-resolved fluoroimmunoassay and ultra-performance liquid chromatography-tandem mass spectrometry indicated that the established fluoroimmunoassay is a reliable method for rapid onsite detection of streptomycin in milk and it has great potential in food safety monitoring.
研究领域:
食品安全
·
免疫学
·
分析化学
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Shaochi Wang,Han Wang,Ting Du,Tong Bu,Jingke Xu,Sijie Liu,Xuechi Yin,Yao Wang,Daohong Zhang,Jing Sun,Jianlong Wang
Antibiotic abuse has caused serious health risks for human beings for long. To address the problem, novel and facile detection techniques are highly desired. Here, an effective multiplex immunochromatographic platform (MICP) with synthesis-free and cost-effective merits is established for simultaneous detection of antibiotics on a single immunochromatographic assay (ICA) strip. Adopting crystal violet (CV) as a signal tag for multiplex ICA allows for direct coupling with multiple antibodies in several minutes. By combining CV and ICA perfectly, this convenient strategy offers improvements in convenience, speed, flexibility, and portability, eventually ensuring the optimized effectiveness of this approach. As a result, the established platform is successfully used to detect streptomycin (STR) and chloramphenicol (CAP) with visual detection mode, and the obtained total recoveries of milk and honey real samples changed from 83.82 to 113.38% with total RSD values of 0.48 to 4.15%.
研究领域:
分析化学
·
食品安全检测
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DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2019
·
IF 9.518
LeLe Li,Xiaoqiang Liu,Liwei Yang,Si Zhang,HeJie Zheng,Yunfei Tang,Danny K.Y. Wong
In this work, a metallic composite with strong electrocatalytic property was designed by uniformly decorating Pt and Sn nanoparticles on the surface of TiO 2 nanorods (Pt– [email protected] 2 ). A detection scheme was then developed based on a dual signal amplification strategy involving the Pt– [email protected] 2 composite and exonuclease assisted target recycling. The Pt– [email protected] 2 composite exhibited an enhanced oxygen reduction current owing to the synergistic effect between Pt and Sn, as well as high exposure of Pt (111) crystal face. Initially, a Pt– [email protected] 2 modified glassy carbon electrode produced an amplified electrochemical signal for the reduction of dissolved oxygen in the analyte solution. Next, a DNA with a complementary sequence to a streptomycin aptamer (cDNA) was immobilised on the Pt– [email protected] 2 modified electrode , followed by the streptomycin aptamer that hybridised with cDNA. The corresponding oxygen reduction current was diminished by 51% attributable to the hindrance from the biomolecules . After a mixture of streptomycin and RecJ f exonuclease was introduced, both the streptomycin-aptamer complex and the cDNA were cleaved from the electrode, making the Pt–Sn and Pt (111) surface available for oxygen reduction. RecJ f would also release streptomycin from the streptomycin-aptamer complex, allowing it to complex again with aptamers on the electrode. This has then promoted a cyclic amplification of the oxygen reduction current by 85%, which is quantitatively related to streptomycin. Under optimal conditions, the aptasensor exhibited a linear range of 0.05–1500 nM and a limit of detection of 0.02±0.0045 nM streptomycin. The sensor was then used in the real-life sample detection of streptomycin to demonstrate its potential applications to bioanalysis .
研究领域:
分析化学
·
生物传感
·
材料科学
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PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Haishuai Peng,Yuanyuan Hui,Lei Zhang,Fuxin Zhang,Yufang Liu,Jianbin Zheng,Rong Jia,Yuxuan Song,Bini Wang
Streptomycin (STR) residue excess in animal-derived foods can cause severe side-effects, such as ototoxicity and nephrotoxicity. Herein, a novel electrochemical aptasensor based on Ti 3 C 2 and metal–organic frameworks (MOFs) nanomaterials was developed for the rapid detection of STR in milk samples. The nanocomposites were synthesized using an impregnation method, and their morphology and structure were characterized via several characterization methods. The nanocomposites exhibited excellent electrochemical properties. Under the optimal conditions, the aptasensor offered a wide STR detection range from 0.01 to 200 nM with a lower limit of detection of 0.0033 nM. Furthermore, the aptasensor demonstrated remarkable reproducibility, stability, and selectivity and was successfully applied for the STR detection in milk samples with satisfactory recoveries ranging from 94.0% to 105.0%. The proposed method can be considered a new and effective method for the rapid detection of STR. Moreover, it provides a new sensing platform for the detection of other toxins.
研究领域:
分析化学
·
食品科学
·
生物医学工程
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高影响因子
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