JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2024
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IF 5.7
Junwei Huang,Zilin Zhu,Ruomu Chen,Dandan Pan,Qing X. Li,Xiangwei Wu
Ibuprofen, a widely used nonsteroidal anti-inflammatory drug, contaminates agricultural products and potentially threatens human health due to its frequent detection and poor biodegradability. Microbial metabolism dominates the elimination of residual ibuprofen in the environment. In mineral salt medium at pH 6 with 5 mM glucose, Streptomyces sp. D218 transformed ibuprofen concentrations ranging from 0.05 to 0.40 mM in 24 h. The optimal temperature, pH, and initial OD600 nm for ibuprofen transformation by strain D218 were 25–37 °C, 5.0–6.0, and 1.0–1.5, respectively. Strain D218 could simultaneously transform ibuprofen into the intermediates 2-hydroxyibuprofen and ibuprofen amide (IBUA). The two intermediates were further metabolized to 2-hydroxyibuprofen amide (2HIBUA), thus relieving the growth inhibition of ibuprofen in Scenedesmus obliquus. This is the first complete pathway reported for the detoxification of ibuprofen transformation by a Gram-positive strain. These findings further our understanding of the microbial catabolism of the IBU.
研究领域:
环境微生物学
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药物代谢
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生态毒理学
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DOI
PubMed
高影响因子
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
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2020
·Vol. 86
·期 16
·
IF 4.016
Han Li,Jiaxiu Wei,Jianxin Dong,Yudong Li,Yongquan Li,Yinghu Chen,Wenjun Guan
Streptomyces is well known for biosynthesis of secondary metabolites with diverse bioactivities. Although oils have been employed as carbon sources to produce polyketide antibiotics for several industrial Streptomyces strains, the intrinsic correlation between oil utilization and high production of antibiotics still remains unclear. In this study, we investigated the correlation between oil metabolism and salinomycin biosynthesis in Streptomyces albus ZD11, which employs soybean oil as the main carbon source. Comparative genomic analysis revealed the enrichment of genes related to triacylglycerol (TAG) metabolism in S. albus ZD11. Transcriptomic profiling further confirmed the enhancement of TAG metabolism and acyl coenzyme A biosynthesis in S. albus ZD11. Multiple secreted lipases, which catalyze TAG hydrolysis, were seen to be working in a synergistic and complementary manner in aiding the efficient and stable hydrolyzation of TAGs. Together, our results suggest that enhanced TAG hydrolysis and fatty acid degradation contribute to the high efficiency of oil utilization in S. albus ZD11 in order to provide abundant carbon precursors for cell growth and salinomycin biosynthesis.IMPORTANCE In order to obtain high-level production of antibiotics, oils have been used as the main carbon source for some Streptomyces strains. Based on multiomics analysis, this study provides insight into the relationship between triacylglycerol (TAG) metabolism and antibiotic biosynthesis in S. albus ZD11, an oil-preferring industrial Streptomyces strain. Our investigation into TAG hydrolysis yielded further evidence that this strain utilizes complicated strategies enabling an efficient TAG metabolism. In addition, a novel secreted lipase was identified that exhibited highly hydrolytic activity for medium- and long-chain TAGs. Our findings represent a good start toward clarifying the complicated relationship between TAG catabolism and high-level antibiotic production in the industrial strains.
研究领域:
微生物学
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生物化学
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DOI
PubMed
高影响因子
MOLECULES
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2016
·Vol. 21
·期 8
·970
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IF 2.465
Ying Han,Erli Tian,Dongbo Xu,Min Ma,Zixin Deng,Kui Hong
During our search for interesting bioactive secondary metabolites from mangrove actinomycetes, the strainStreptomycessp. 219807 which produced a high elaiophylin yield of 4486 mg/L was obtained. A new elaiophylin derivative, halichoblelide D (1), along with seven known analogues2–8was isolated and identified from the culture broth. Their chemical structures were determined by detailed analysis of 1D and 2D NMR and HRMS data. The absolute configuration of halichoblelide D (1) was confirmed by comparing the CD spectrum with those of the reported analogues. Compounds1–7exhibited potent cytotoxic activities against HeLa and MCF-7 cells with IC50values ranging from 0.19 to 2.12 μM.Keywords:mangrove actinomycetes;Streptomycessp. 219807;elaiophylin;cytotoxic
研究领域:
微生物学
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药物化学
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肿瘤学
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DOI
PubMed
高影响因子
Microorganisms
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2020
·Vol. 8
·期 3
·319
·
IF 4.152
Xuewei Li,Beibei Li,Shurui Cai,Yu Zhang,Mingjie Xu,Chunmei Zhang,Bo Yuan,Ke Xing,Sheng Qin
Black spot disease, which is caused by the pathogenic fungalCeratocystis fimbriata, seriously affects the production of sweet potato and its quality during postharvest storage. In this study, the preliminary identification of the rhizosphere actinomycete strain SPS-33, and its antifungal activity of volatilesin vitroandin vivowas investigated. Based on morphological identification and phylogenetic analysis of the 16S rRNA gene sequence, strain SPS-33 was identified asStreptomyces lavendulae.Volatile organic compounds (VOCs) emitted by SPS-33 inhibited mycelial growth and sporulation ofC. fimbriatain vitroand also induced a series of observable hyphae morphological changes. In anin vivopathogenicity assay, exposure to SPS-33 significantly decreased the lesion diameter and water loss rate in sweet potato tuberous roots (TRs) inoculated withC. fimbriata. It increased the antioxidant enzymes’ activities of peroxidase, catalase, and superoxide dismutase as well as decreased malondialdehyde and increased total soluble sugar. In the VOC profile of SPS-33 detected by a headspace solid-phase micro extraction (HS-SPME) and gas chromatography-mass spectrometry (GC-MS), heptadecane, tetradecane, and 3-methyl-1-butanol were the most abundant compounds. 2-Methyl-1-butanol, 3-methyl-1-butanol, pyridine, and phenylethyl alcohol showed strong antifungal effects againstC. fimbriata. These findings suggest that VOCs fromS. lavendulaeSPS-33 have the potential for pathogenC. fimbriatacontrol in sweet potato postharvest storage by fumigant action.Keywords:Ipomoea batatas(L.) Lam.;Streptomyces lavendulae;volatile organic compound;fumigant;antimicrobial
研究领域:
植物病理学
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微生物学
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DOI
PubMed
高影响因子
Phytopathology Research
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2025
·Vol. 7
·期 1
·1-16
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IF 3.5
He Ya-Wen,Law Jodi Woan-Fei,Azad Sepideh Mazhari,Hu Wen-Da,Song Kai,Chua Kah-Ooi,Jiang Lian,Jin Yu,Lee Learn-Han,Zhou Lian
Bacterial leaf blight (BLB), caused by Xanthomonas oryzae pv. oryzae (Xoo), is one of the most severe bacterial diseases in rice. The current BLB-prevention strategy depends on chemical antimicrobials. The biological control methods have gained considerable attention. Among them, Streptomyces are particularly promising candidates due to their ability to produce diverse natural antimicrobial and plant-growth-promoting metabolites. In this study, we isolated a Streptomyces strain HSW2009 from the rice rhizosphere. This strain displayed significant antagonistic activity against Xoo. The antagonistic metabolite was extracted and purified from the HSW2009 culture. High-performance liquid chromatography-mass chromatography and nuclear magnetic resonance analyses revealed that the active compound is Piericidin A1, a member of the piericidin family metabolites containing a 4-pyridinol core linked with a methylated polyketide side chain. Piericidin A1 was shown to protect rice from Xoo infection in the microclimate chamber. The strain HSW2009 produced pale yellow aerial mycelia on the agar plate of the International Streptomyces Project-2 medium. Its cellular morphology conformed to that typically observed in the genus Streptomyces . Phylogenetic analysis of 16S rRNA gene sequences showed that HSW2009 was closely related to Streptomyces zagrosensis , S. youssoufiensis , and S. varsoviensis . HSW2009 displayed a unique DNA profile in BOX-PCR fingerprinting analysis and had a genome size of 8,806,972 bp, with a 72.83% G + C content. Average nucleotide identity analysis and digital DNA–DNA hybridization using the Type Strain Genome Server supported HSW2009 as a novel Streptomyces sp. It was therefore proposed as Streptomyces shaowuensis sp. nov., type strain HSW2009.
研究领域:
微生物学
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植物病理学
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基因组学
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DOI
高影响因子
CHINESE SCIENCE BULLETIN
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2013
·Vol. 58
·期 26
·3266-3273
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IF 1.319
Wu Qiong,Bai LinQuan,Liu WeiCheng,Li YingYing,Lu CaiGe,Li YaQian,Lin ZhenYa,Wang Meng,Xue ChunSheng,Chen Jie
Streptomyces lydicus A01 resists many plant pathogens (including Fusarium spp.) by producing the antifungal agent natamycin, which binds to the ergosterol of fungal cell membranes and inhibits the growth of pathogens. Trichoderma harzianum CECT2413 is a widely-distributed soil fungus that antagonizes several plant fungal pathogens (including Fusarium spp.) by producing chitinase and degrading chitin, a major component of the fungal cell wall. This study attempted to enhance the biocontrol effect of S. lydicus A01 on Fusarium spp. by transforming the chitinase gene of Trichoderma . Chitinase and natamycin could act synergistically on both the cell walls and cell membranes of pathogens. The 33-kD chitinase-encoding gene ( chit33 ) was cloned and conjugal-transformed from T. harzianum CECT2413 to S. lydicus A01, and then confirmed via polymerase chain reaction (PCR) assays. Subsequent analyses using the 3,5-dinitrosalicylic acid (DNS) method and ultraviolet spectrophotometry showed that compared with its wild type strain (WT), the S. lydicus A01 conjugal transformant (CT) with chit33 gene exhibited substantially higher chitinase activity and natamycin production. The resistance of S. lydicus A01- chit33 CT and WT to four Fusaria in crops and vegetables was tested via the cup-plate method. Compared with the WT, the conjugal transformant of S. lydicus A01 with chit33 gene from T. harzianum CECT2413 showed greatly increased biocontrol effect on fusarium disease. This study would be beneficial to the development of high-quality antifungal bio-agents for agricultural applications via the synergy between the previously non-existent and pre-existing functions achieved through heterogeneous gene transformation.
研究领域:
微生物学
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植物病理学
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分子生物学
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DOI
高影响因子
JOURNAL OF ANTIBIOTICS
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2016
·Vol. 70
·期 2
·216-218
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IF 2.173
Gao Mei-yue,Qi Huan,Li Jian-song,Zhang Hui,Zhang Ji,Wang Ji-dong,Xiang Wen-sheng
Accessions GenBank/EMBL/DDBJ AF389344 KX118440
研究领域:
微生物学
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天然产物化学
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产品关联
DOI
PubMed
高影响因子
Genes
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2017
·Vol. 8
·期 12
·346
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IF 3.6
Guoqiang Zhang,Dailin Yu,Bu Sang,Juntao Feng,Lirong Han,Xing Zhang
Streptomyces kanasensisZX01 produces some antibiotics and a glycoprotein with antiviral activity. To further evaluate its biosynthetic potential, here we sequenced the 7,026,279 bp draft genome ofS. kanasensisZX01 and analyzed all identifiable secondary gene clusters for controlling natural products. More than 60 putative clusters were found inS. kanasensisZX01, the majority of these biosynthetic loci are novel. In addition, the regulators for secondary metabolism inS. kanasensisZX01 were abundant. The global regulatornsdAnot only controls biosynthesis of some antibiotics, but also enhances production of glycoprotein GP-1 with antiviral activity. This study importantly reveals the powerful interplay between genomic analysis and studies of traditional natural product purification/production increasing.Keywords:genome mining;secondary metabolism;nsdA;regulator;glycoprotein GP-1
研究领域:
微生物学
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基因组学
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药理学
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DOI
PubMed
高影响因子
Bioresources and Bioprocessing
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2017
·Vol. 4
·期 1
·1-7
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IF 0
Jiang Ying,Shang Yue-Peng,Li Hao,Zhang Chao,Pan Jiang,Bai Yun-Peng,Li Chun-Xiu,Xu Jian-He
To improve the fermentation production of transglutaminase (TGase) from Streptomyces mobaraensis for applications in the food industry, the atmospheric and room-temperature plasma (ARTP) mutagenesis was applied to breed S. mobaraensis mutants with increased TGase production.After eight rounds of iterative ARTP mutagenesis, four genetically stable mutants, Sm5-V1, Sm6-V13, Sm2-V10, and Sm7-V12, were identified, which showed increased TGase production by 27, 24, 24, and 19%, respectively. The best mutant Sm5-V1 exhibited a maximum TGase activity of 5.85 U/mL during flask fermentation. Compared to the wild-type strain, the transcription levels of the zymogen TGase genes in the mutants increased significantly as indicated by quantitative real-time PCR, while the gene nucleotide sequences of the mutants did not change at all. It was shown that the overexpression of TGase zymogen gene in the mutants contributes to the increase in TGase production.ARTP is a potentially efficient tool for microbial mutation breeding to bring some significant changes required for the industrial applications.
研究领域:
微生物学
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食品科学
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工业生物技术
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DOI
PubMed
ACS Omega
·
2023
·
IF 4.1
Kunyu Xia,Jiaxu Shang,Jiwen Sun,Weiming Zhu,Peng Fu
To expand the chemical diversity of secondary metabolites produced by two marine-derived enterocin- and wailupemycin-producing Streptomyces strains, OUCMDZ-3434 and OUCMDZ-2599, precursor feeding and solid fermentation strategies were used. Two new compounds, wailupemycins Q (1) and R (2), were isolated from the extracts of liquid and solid fermentation of OUCMDZ-3434. Furthermore, during the fermentation of OUCMDZ-3434, p-fluorobenzoic acid was added as the key biosynthetic precursor, which resulted in the isolation of eight new fluorinated enterocin and wailupemycin derivatives (3–10) and 10 previously reported analogues (11–20). From the solid fermentation extract of OUCMDZ-2599, a new sulfur-containing compound thiotetromycin B (21) and its known analogue thiotetromycin (22) were identified. Moreover, the solid fermentation strategy effectively activated the biosynthesis of siderophores (23–25) of strain OUCMDZ-2599. Compound 3 showed moderate antibacterial activity against Staphylococcus aureus and methicillin-resistant Staphylococcus aureus subsp. aureus with MIC values of 4 μg/mL. Compounds 23–25 were significantly capable of binding Fe(III).
研究领域:
微生物学
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药物化学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
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2022
·Vol. 24
·期 1
·275
·
IF 6.208
Fei Li,Honglu Zhao,Yuxin Liu,Jiaqi Zhang,Hongbo Yu
Lytic polysaccharide monooxygenases (LPMOs) have the potential to improve recalcitrant polysaccharide hydrolysis by the oxidizing cleavage of glycosidic bond.Streptomycesspecies are major chitin decomposers in soil ecological environments and encode multiplelpmogenes. In this study, we demonstrated that transcription of thelpmogene,Sclpmo10G, in theStreptomyces coelicolorA3(2) (ScA3(2)) strain is strongly induced by chitin. TheScLPMO10G protein was further expressed inEscherichia coliand characterized in vitro. TheScLPMO10G protein showed oxidation activity towards chitin. Chitinase synergy experiments demonstrated that the addition ofScLPMO10G resulted in a substantial in vitro increase in the reducing sugar levels. Moreover, in vivo the LPMO-overexpressing strainScΔLPMO10G(+) showed stronger chitin-degrading ability than the wild-type, leading to a 2.97-fold increase in reducing sugar level following chitin degradation. The total chitinase activity ofScΔLPMO10G(+) was 1.5-fold higher than that ofScA3(2). In summary,ScLPMO10G may play a role in chitin biodegradation inS. coelicolor, which could have potential applications in biorefineries.Keywords:lytic polysaccharide monooxygenases;transcription;expression;Streptomyces coelicolorA3(2);chitin biodegradation
研究领域:
微生物学
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生物化学
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DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
·
2021
·
IF 9.642
Cheng Wang,Xi Chen,Yingying Jiang,Na Li,Ping Zhu,Hong Xu
Facile and green fabrication of reduced graphene oxide on loofah sponge (rGOLS) carrier was applied for cell immobilization of ε-Poly- l -lysine (ε-PL) production. Due to surface properties including large specific surface area, high polarity, and low interaction energy, rGOLS-1 was employed as the optimum rGOLS to enhance immobilization of Streptomyces albulus . Compared with raw LS, batch experiments showed rGOLS-1 facilitated superior cell vitality for ε-PL production due to the presence of reduced graphene oxide. In the sequential fed-batch cultivation of Streptomyces albulus using rGOLS-1 with an aerobic plant fibrous-bed bioreactor (rGOLS-1-AFPB), the maximum ε-PL concentration and productivity reached to 39.2 ± 0.63 g/L and 0.48 g/L/h. The cells immobilized in rGOLS-1 with high vitality and ε-PL production efficiency were reused six times over a period of 624 h. This research afforded an effective approach to enhance the fermentation performance of immobilized cells with the design of an advanced immobilization carrier.
研究领域:
生物工程
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微生物学
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产品关联
DOI
PubMed
高影响因子
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