INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2022
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IF 8.025
Shuo Shi,Xi Lan,Jianxing Feng,Yongsheng Ni,Mingqiang Zhu,JingSun,Jianlong Wang
Designing wound dressing materials with hemocompatibility, suitable mechanical properties, outstanding hemostatic effects and anti-inflammatory activity is of great practical significance for wound management. Herein, a hemostatic hydrogel loaded with Lycium barbarum L. polysaccharide (LBP)-functionalized ultrathin MMT nanosheets (L-MMT NSs) was fabricated for efficient hemostasis and wound healing . Loading the L-MMT NSs into polyvinyl alcohol (PVA), the obtained P-L-MMT hydrogel exhibited a 3D porous structure with good swelling properties, cytocompatibility, hemocompatibility, and anti-inflammatory activity. Importantly, in vivo investigations demonstrated that the P-L-MMT hydrogel exerts outstanding hemostasis activity in the hemorrhaging mouse liver model and reduces tissue damage caused by inflammation to shorten wound healing time. Altogether, the convenient exfoliation and functionalization of bulk MMT using LBPs make this inexpensive and rising nanostructure more attractive in the application of nanomedicine. Moreover, due to the synergy between hemostasis and anti-inflammation, this newly developed multifunctional P-L-MMT hydrogel represents a promising material in biomedical fields.
研究领域:
生物材料学
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医学
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纳米医学
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DOI
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
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2023
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IF 6
Zhonghai Tang,Yuan Xu,Changyong Cai,Zhijian Tan
Due to the nonvolatility of deep eutectic solvents (DESs), the recycling and reuse of them is encountering a lot of problems in the extraction and separation field. In this study, temperature-switchable DESs (TS-DESs) were prepared and their temperature-responsive characteristics and phase diagram were studied. TS-DESs were further used as the recyclable extractants for extracting Lycium barbarum polysaccharides (LBP) below their lower critical solution temperature (LCST), and then they can be recovered from the extract above their LCST. The main factors influencing the extraction process were investigated in the single-factor experiments. A maximal extraction yield of 465 mg/g was obtained. The TS-DES was recovered and reused in recycling experiments. The recovery of TS-DES was 80.2 % after the fifth cyclic test. The obtained LBP was studied by determining its molecular weight, assessing its radical scavenging activity, and analyzing its monosaccharide constituents. This study presents a novel method for polysaccharide extraction, which can also provide guidance for bioactive compounds extraction using recyclable extractants.
研究领域:
植物化学
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食品科学
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环境科学
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产品关联
DOI
高影响因子
Natural Products and Bioprospecting
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2025
·Vol. 15
·期 1
·1-8
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IF 4.9
Wang Zi-Jiao,Tan Bang-Yin,Zhao Yun,Wang Chang-Bin,Zhao Yun-Li,Luo Xiao-Dong
Two new together with 32 known compounds were isolated from the leaves of Lycium barbarum . Their structures were elucidated using 1D and 2D NMR, HRESIMS, and ECD spectroscopic techniques. Compounds 1–34 were evaluated for their anti-rheumatoid arthritis activities in a lipopolysaccharide (LPS)-induced MH7A cells inflammatory model. As a result, compounds 1– 3 , 6 , 8 , 10 , 14 , 17–19 , 29 and 31 inhibited the activity of lactate dehydrogenase (LDH) and nitric oxide (NO) at concentrations 20 μM. Among them, compound 1 showed the best effectiveness, with inhibition rates of 46.7% for NO and 32.8% for LDH. Graphical
研究领域:
药理学
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植物化学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Ya-Fang Shang,Bi-Ming Ding,Zhi-Jing Ni,Kiran Thakur,Jian-Guo Zhang,Zhao-Jun Wei
Present study aimed to identify the flavour substances and key precursor peptides of meaty flavour prepared from Maillard reaction products (MRPs) of Lycium barbarum seed meal. The MRPs from L. barbarum seed meal exhibited strong meat flavour, were produced at 120 °C for 120 min after addition of 0.15 g cysteine. A total of 69 volatile flavour compounds were identified, with the PF2-MRPs group containing the highest levels of strong meaty flavour compounds,including sulphur-containing compounds such as 3-Methyl-2-thiophene carboxaldehyde, 2-Furfurylthiol, and 2-Acetylfuran, up to 1110.0 ng/g. The peptide fractions were also analysed by LC-MS/MS before and after the Maillard reaction, and the 10 key precursor peptides with the most significant differences were identified..Among them, EPNLALPLP and QAGPQ were the most consumed in the Maillard reaction. This study revealed the role of key precursor peptides in the Maillard reaction, providing a scientific basis for the development of new natural flavour enhancers.
研究领域:
食品科学
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生物化学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2022
·
IF 8.025
Jixian Zhang,Tao Ji,Xue Yang,Guoyan Liu,Li Liang,Xiaofang Liu,Chaoting Wen,Zhiqiang Ye,Maowei Wu,Xin Xu
Selenium nanoparticles (SeNPs) in an aqueous solution have poor stability and tend to aggregate when stored for a long time. In the present study, SeNPs were stabilized by using Lycium barbarum polysaccharide (LBP) and Lycium barbarum protein (LBPr) conjugates (LBPP) as a stabilizer and dispersing agent. Particularly, the LBPP 1 was obtained with subcritical water treatment. In addition, the physical stability, re-dispersity and antitumor activity of LBPP 1 -SeNPs were investigated. The results showed the particle size of LBPP 1 -SeNPs was maintained at 111.5–117 nm, which was stable at PH 6, 4 °C and darkness for at least 40 days. Besides, the result of TEM showed that the dispersion of LBPP 1 -SeNPs had more clear layers and smoother surfaces. Moreover, LBPP 1 -SeNPs had excellent re-dispersibility and exhibited a significant inhibitory effect on HepG-2 cells and Caco-2 cells, respectively ( p < 0.05). Therefore, LBPP 1 -SeNPs can be used as potential selenium nutritional supplements for food and medical applications.
研究领域:
食品科学
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生物医学工程
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材料科学
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产品关联
DOI
PubMed
高影响因子
Antioxidants
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2024
·Vol. 13
·期 7
·859
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IF 6
Ruifeng Wang,Anping Ding,Jiaye Wang,Jiaxue Wang,Yujie Zhou,Miao Chen,Shuang Ju,Mingpu Tan,Zengxu Xiang
Astragalin (AG), a typical flavonoid found inThesium chinenseTurcz (T. chinense), is abundant in various edible plants and possesses high nutritional value, as well as antioxidant and antibacterial effects. In this study, we initially predicted the mechanism of action of AG with two anti-aging and antioxidant-related protein targets (CD38 and IGFR) by molecular docking and molecular dynamics simulation techniques. Subsequently, we examined the anti-aging effects of AG inCaenorhabditis elegans(C. elegans), the antioxidant effects in zebrafish, and verified the related molecular mechanisms. InC. elegans, AG synergistically extended the lifespan ofC. elegansby up-regulating the expression ofdaf-16through inhibiting the expression ofdaf-2/IGFRand also activating the AMPK and MAPK pathways to up-regulate the expression ofsir-2.1,sir-2.4, andskn-1. In oxidatively damaged zebrafish embryos, AG demonstrated a synergistic effect in augmenting the resistance of zebrafish embryos to oxidative stress by up-regulating the expression levels ofSIRT1andSIRT6within the zebrafish embryos system via the suppression of CD38 enzymatic activity and then inhibiting the expression ofIGFRthrough high levels ofSIRT6. These findings highlight the antioxidant and anti-aging properties of AG and indicate its potential application as a supplementary ingredient in aquaculture for enhancing fish health and growth.
研究领域:
分子生物学
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老年医学
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营养科学
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水产养殖
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产品关联
DOI
PubMed
高影响因子
Chemistry Central Journal
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2017
·Vol. 11
·期 1
·1-8
·
IF 2.442
Xie Yulu,Li Xican,Xu Jieying,Jiang Qian,Xie Hong,He Jianfeng,Chen Dongfeng
Background
Suoyang originates from a psammophyte named Cynomorium songaricum Rupr and has been known as a phenolic-antioxidant-enriched traditional Chinese herbal medicine. The present study attempted to investigate the protective effect of phenolic antioxidants in Suoyang towards •OH-mediated MSCs and then further discusses the chemical mechanisms.Methods The lyophilized aqueous extract of Suoyang (LAS) was prepared and characterized using HPLC. Then, two phenolic antioxidant references, epicatechin and luteolin-7-O-β-D-glucoside, along with LAS, were investigated for their effects on the viability of •OH-treated MSCs using the 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyl (MTT) assay. The comparison and mechanistic chemistry of epicatechin and luteolin-7-O-β-D-glucoside were further explored using various antioxidant assays, including PTIO•-scavenging, FRAP (ferric ion reducing antioxidant power), ABTS+•-scavenging, and DPPH•-scavenging. Their Fe2+-binding capacities were also compared using ultraviolet (UV) spectra.Results The HPLC analysis indicated that there are 8 phenolic antioxidants in LAS, including epicatechin, luteolin-7-O-β-D-glucoside, gallic acid, protocatechuic acid, catechin, isoquercitrin, phlorizin, and naringenin. The MTT assay revealed that epicatechin could more effectively increase the survival of •OH-treated MSCs than luteolin-7-O-β-D-glucoside. Similarly, epicatechin exhibited higher antioxidant abilities than luteolin-7-O-β-D-glucoside in the DPPH•-scavenging, ABTS+•-scavenging, FRAP, and PTIO•-scavenging assays. In the Fe2+-binding assay, luteolin-7-O-β-D-glucoside gave a stronger UV peak at 600 nm, with ε = 2.62 × 106 M−1 cm−1, while epicatechin produced two peaks at 450 nm (ε = 8.47 × 105 M−1 cm−1) and 750 nm (ε = 9.68 × 105 M−1 cm−1).Conclusion As two reference antioxidants in Suoyang, epicatechin and luteolin-7-O-β-D-glucoside can enhance the viability of •OH-damaged MSCs. Such a beneficial effect may be from their antioxidant effects, including direct-antioxidant and indirect-antioxidant (i.e., Fe2+-binding) processes. In the direct-antioxidant process, proton (H+), one electron (e), or even hydrogen-atom (•H) transfer may occur to fulfill radical-scavenging (especially •OH-scavenging); in this aspect, epicatechin is superior to luteolin-7-O-β-D-glucoside due to the presence of more phenolic –OHs. The additional –OHs can also be responsible for the better cytoprotective effect. In terms of indirect-antioxidant potential, however, epicatechin is inferior to luteolin-7-O-β-D-glucoside due to the absence of a hydroxyl-keto moiety. These findings will provide new information about medicinal psammophytes for MSC transplantation.
研究领域:
药理学
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药物化学
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细胞生物学
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中医药
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产品关联
DOI
PubMed
高影响因子
Frontiers in Plant Science
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2025
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IF 4.8
Jiacheng Ji,Xinxin Wei,Huan Guan,Zikang Jin,Xin Yue,Zhuoran Jiang,Youla Su,Shuying Sun,Guilin Chen
The efficacy of traditional Chinese medicine is determined by its bioactive components, which exhibit variability depending on environmental conditions and hereditary influences. In this study, we focus on Cynomorium songaricum Rupr., a medicinally significant species facing sustainability challenges. However, the ecological drivers governing its distribution, as well as the relationship between environmental factors and bioactive components, remain unclear. Thus, we sampled 28 representative distribution areas of C. songaricum in China. Employing Maximum Entropy (MaxEnt) modeling, we projected current and future (2050s-2090s) habitat suitability under four emission scenarios. Notably, species distribution exhibited expansion (8.03%-29.06% range increase across scenarios) with precipitation of the wettest month (BIO13) and soil pH emerging as key drivers (combined contribution >49%). Ultra-performance liquid chromatography (UPLC) fingerprinting combined with machine learning regression was applied to quantify six key bioactive components in C. songaricum, 3,4-dihydroxybenzaldehyde, catechin, epicatechin, ursolic acid, total phenolics, and crude polysaccharides—revealing significant concentration variations among geographically distinct populations. Slope gradient (slope), min temperature of coldest month (BIO6), precipitation of coldest quarter (BIO19), sunshine duration in growing season(hsdgs), and isothermality (BIO3) were identified as key regulatory factors influencing the accumulation of multiple components. Specifically, slope acted as a key shared negative regulator for 3,4-dihydroxybenzaldehyde, catechin, and crude polysaccharides. BIO6 served as a key shared positive regulator for catechin and total phenolics, while functioning as a key negative regulator for ursolic acid. BIO19 was identified as a key shared negative regulator for catechin and epicatechin. Hsdgs acted as a key positive regulator for ursolic acid while negatively regulating crude polysaccharides. Additionally, BIO3 served as a key shared positive regulator for both ursolic acid and total phenolics. This study provides the scientific basis for enabling targeted cultivation zones that balance therapeutic compound yield with arid ecosystem conservation.
研究领域:
生态学
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植物学
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药用植物学
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环境科学
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机器学习
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生物地理学
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植物化学
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气候变化影响
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保护生物学
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产品关联
DOI
PubMed
高影响因子
PHYTOMEDICINE
·
2024
·
IF 6.7
Zhihao Liu,Qixiang Shang,Huali Zuo,Haimeng Li,Daozheng Fang,Jiayu Zhang,Hsien-Da Huang,Daniel Granato,Jianping Chen,Jihang Chen
Background Type 2 diabetes mellitus (T2DM) is a chronic metabolic disorder characterized by elevated blood glucose levels, posing a significant global health concern due to its increasing prevalence. Insulin resistance (IR) plays a major role in the development of T2DM and is often linked to factors such as obesity, physical inactivity, and a sedentary lifestyle. Recently, there has been growing interest in exploring the potential of natural products for improving insulin sensitivity and glucose metabolism. Among these, Cynomorium songaricum Rupr., an edible parasitic plant, has shown promising antidiabetic effects. However, research on its beneficial effects on IR is still nascent. Therefore, this study aims to investigate the application of a Cynomorium songaricum flavonoid-enriched fraction (CSF) in the treatment of IR in T2DM, along with elucidating the chemical and biochemical mechanisms involved. Method First, UHPLC/ESI-LTQ-Orbitrap-MS was utilized to perform a chemical profiling of Cynomorium songaricum extract (CSF). Subsequently, glycogen synthesis, gluconeogenesis and glucose consumption assays were conducted on HepG2 cells with a high glucose high insulin -induced IR model to illustrate the favorable impacts of CSF on IR. Then, an innovative network pharmacology analysis was executed to predict the potential chemical components and hub genes contributing to CSF's protective effect against IR. To further elucidate molecular interactions, molecular docking studies were performed, focusing on the binding interactions between active constituents of CSF and crucial targets. Additionally, an RNA-sequencing assay was employed to uncover the underlying biochemical signaling pathway responsible for CSF's beneficial effects. To validate these findings, western blot and qPCR assays were employed to verify the pathways related to IR and the potential signaling cascades leading to the amelioration of IR. Results The UHPLC/ESI-LTQ-Orbitrap-MS analysis successfully identified a total of thirty-six flavonoids derived from CSF. Moreover, CSF was shown to significantly improve glycogen synthesis and glucose consumption as well as inhibit gluconeogenesis in HepG2 cells of IR. An innovative network pharmacology analysis unveiled key hub genes—AKT1 and PI3K—integral to metabolic syndrome-related signaling pathways, which contribute to the favorable impact of CSF against IR. Noteworthy active ingredients including quercetin, ellagic acid and naringenin were identified as potential contributors to these effects. The results of western blot and qPCR assays provided compelling evidence that CSF improved insulin sensitivity by modulating the PI3K-Akt signaling pathway. Subsequent RNA-sequencing analysis, in tandem with western blot assays, delved deeper into the potential mechanisms underlying CSF's advantageous effects against IR, potentially associated with the enhancement of endoplasmic reticulum (ER) proteostasis. Conclusion CSF exhibited a remarkable ability to enhance insulin sensitivity in the IR model of HepG2 cells. This was evident through enhancements in glycogen synthesis and glucose consumption, along with its inhibitory impact on gluconeogenesis. Furthermore, CSF demonstrated an improvement in the insulin-mediated PI3K-Akt signaling pathway. The potential active constituents were identified as quercetin, ellagic acid and naringenin. The underlying biochemical mechanisms responsible for CSF's beneficial effects against IR were closely linked to its capacity to mitigate ER stress, thereby offering a comprehensive understanding of its protective action.
研究领域:
药理学
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生物化学
·
分子生物学
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糖尿病研究
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细胞生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）